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The map label for this gene is truB

Identifier: 147675140

GI number: 147675140

Start: 179320

End: 180258

Strand: Direct

Name: truB

Synonym: VC0395_A0176

Alternate gene names: 147675140

Gene position: 179320-180258 (Clockwise)

Preceding gene: 147673152

Following gene: 147675292

Centisome position: 5.93

GC content: 51.76

Gene sequence:

>939_bases
ATGGTTCGTCGACGTAAAGGTCGGGTTATCCACGGTGTGATTCTGTTGGATAAGCCGACAGGTATCTCTTCCAACGACGC
TTTGCAAAAGGTTAAACGCCTCTATGGCGCTGAAAAAGCGGGACATACTGGCGCGCTTGATCCTCTGGCGACGGGCATGT
TGCCAATCTGCCTCGGTGAAGCCACTAAGTTTTCCCAATTTCTGCTCGATTCTGATAAACGTTATCGTGTGATCGCCAAA
TTGGGTGAACGTACCGACACCTCAGATTCTGATGGTCAAGTGGTACAAACGCGTCCTGTTCACGTGGATTACGATACGCT
GCTGGCCTGTATCGCCAAGTTTCGCGGTGAAACGGATCAAGTTCCATCCATGTTTTCAGCGTTGAAATACCAAGGTCGTC
CACTCTACGAATACGCCCGTCAGGGTATTGAAGTGCCGCGTGAAGCGCGCAAGATCACTGTGTATGAGATTGAGCTGCAT
CGCTTTGAAGGTGATGAAGTGGAGATGGAAGTACACTGCTCGAAAGGCACTTATATTCGTACCATAGTCGATGATCTGGG
TGAAATGCTGGGCTGTGGAGCGCATGTGACCATGCTGCGCCGTGTGGGTGTCGCTAACTATCCGTATGAGCGAATGGTGA
CGCTAGAGCAACTCAACGCTTTGGTTGAACAAGCCCATCGTGACGAAAAAGCGGTGGCGGACGTTCTCGATCCGCTATTA
CTGCCGATGGATACTGCCGTGGAAGCTTTACCCGAAGTCAACGTGATCCCAGAACTGATGACACTTATTCAGCATGGGCA
AGCAGTACAGGTTTCGGGGGCACCATCCGACGGTATGGTGCGCATTACTGGCGGCGAGCAGAAGCTATTCCTTGGCGTTG
GCGAGATTGACGATAACGGTAAGGTCGCGCCGAAACGCTTGGTGGTGTACGGCGAATAA

Upstream 100 bases:

>100_bases
CCTTGGTTGAAGGTATGCGTATGTCAAACCTCGTTACCGAAGTGATCAATAGTGACAAGCGCCGCAAGCATGAAGCGGGT
CGTGATGAGGACGAAGCGTA

Downstream 100 bases:

>100_bases
ATTCGTGGAAGGGCTCGCTGTATGGCGAGACATTCCGCTTGTGCGGATTAGGAATATTCCCTATAATCCGCGCTTCTCGT
GTCGGCTGAATCAGAGATTG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MVRRRKGRVIHGVILLDKPTGISSNDALQKVKRLYGAEKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVIAK
LGERTDTSDSDGQVVQTRPVHVDYDTLLACIAKFRGETDQVPSMFSALKYQGRPLYEYARQGIEVPREARKITVYEIELH
RFEGDEVEMEVHCSKGTYIRTIVDDLGEMLGCGAHVTMLRRVGVANYPYERMVTLEQLNALVEQAHRDEKAVADVLDPLL
LPMDTAVEALPEVNVIPELMTLIQHGQAVQVSGAPSDGMVRITGGEQKLFLGVGEIDDNGKVAPKRLVVYGE

Sequences:

>Translated_312_residues
MVRRRKGRVIHGVILLDKPTGISSNDALQKVKRLYGAEKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVIAK
LGERTDTSDSDGQVVQTRPVHVDYDTLLACIAKFRGETDQVPSMFSALKYQGRPLYEYARQGIEVPREARKITVYEIELH
RFEGDEVEMEVHCSKGTYIRTIVDDLGEMLGCGAHVTMLRRVGVANYPYERMVTLEQLNALVEQAHRDEKAVADVLDPLL
LPMDTAVEALPEVNVIPELMTLIQHGQAVQVSGAPSDGMVRITGGEQKLFLGVGEIDDNGKVAPKRLVVYGE
>Mature_312_residues
MVRRRKGRVIHGVILLDKPTGISSNDALQKVKRLYGAEKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVIAK
LGERTDTSDSDGQVVQTRPVHVDYDTLLACIAKFRGETDQVPSMFSALKYQGRPLYEYARQGIEVPREARKITVYEIELH
RFEGDEVEMEVHCSKGTYIRTIVDDLGEMLGCGAHVTMLRRVGVANYPYERMVTLEQLNALVEQAHRDEKAVADVLDPLL
LPMDTAVEALPEVNVIPELMTLIQHGQAVQVSGAPSDGMVRITGGEQKLFLGVGEIDDNGKVAPKRLVVYGE

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=269, Percent_Identity=32.7137546468402, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4503337, Length=252, Percent_Identity=29.7619047619048, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI215599015, Length=252, Percent_Identity=29.7619047619048, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI2367200, Length=306, Percent_Identity=66.6666666666667, Blast_Score=427, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17553978, Length=238, Percent_Identity=29.8319327731092, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=191, Percent_Identity=30.3664921465969, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=242, Percent_Identity=29.7520661157025, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281364189, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364187, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364185, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364183, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI62471759, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17975520, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_VIBC3 (A5F928)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F928
- SMR:   A5F928
- STRING:   A5F928
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0176
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK05033
- BioCyc:   VCHO345073:VC0395_A0176-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 34516; Mature: 34516

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRRRKGRVIHGVILLDKPTGISSNDALQKVKRLYGAEKAGHTGALDPLATGMLPICLGE
CCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCC
ATKFSQFLLDSDKRYRVIAKLGERTDTSDSDGQVVQTRPVHVDYDTLLACIAKFRGETDQ
HHHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCCEEEECCCEECHHHHHHHHHHHCCCCCH
VPSMFSALKYQGRPLYEYARQGIEVPREARKITVYEIELHRFEGDEVEMEVHCSKGTYIR
HHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCEEEEEEEECCCCCHH
TIVDDLGEMLGCGAHVTMLRRVGVANYPYERMVTLEQLNALVEQAHRDEKAVADVLDPLL
HHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LPMDTAVEALPEVNVIPELMTLIQHGQAVQVSGAPSDGMVRITGGEQKLFLGVGEIDDNG
CCHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCEEEEEEECEECCCC
KVAPKRLVVYGE
CCCCEEEEEECC
>Mature Secondary Structure
MVRRRKGRVIHGVILLDKPTGISSNDALQKVKRLYGAEKAGHTGALDPLATGMLPICLGE
CCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHCC
ATKFSQFLLDSDKRYRVIAKLGERTDTSDSDGQVVQTRPVHVDYDTLLACIAKFRGETDQ
HHHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCCEEEECCCEECHHHHHHHHHHHCCCCCH
VPSMFSALKYQGRPLYEYARQGIEVPREARKITVYEIELHRFEGDEVEMEVHCSKGTYIR
HHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCEEEEEEEECCCCCHH
TIVDDLGEMLGCGAHVTMLRRVGVANYPYERMVTLEQLNALVEQAHRDEKAVADVLDPLL
HHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LPMDTAVEALPEVNVIPELMTLIQHGQAVQVSGAPSDGMVRITGGEQKLFLGVGEIDDNG
CCHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCEEEEEEECEECCCC
KVAPKRLVVYGE
CCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA