Click here to switch to the map view.

The map label for this gene is aceF [H]

Identifier: 147674758

GI number: 147674758

Start: 2136219

End: 2138132

Strand: Reverse

Name: aceF [H]

Synonym: VC0395_A1989

Alternate gene names: 147674758

Gene position: 2138132-2136219 (Counterclockwise)

Preceding gene: 147675572

Following gene: 147674273

Centisome position: 70.7

GC content: 52.19

Gene sequence:

>1914_bases
TTGAAGGTAGGAAAAGTAATGGCAATCGAAATTTATGTACCTGACATCGGTGCGGATGAGGTTGAAGTCACTGAGATTCT
CGTCAAAGTCGGCGACAAAGTGGCTGAAGAGCAATCTCTGATCACGGTTGAAGGCGACAAAGCTTCTATGGAAGTTCCTG
CGTCTCAAGCGGGTATCGTTAAAGAGATCAAAGTAGTGGCAGGTGACAAAGTGTCTACTGGCTCTCTGATCATGGTCTTT
GAAGCGGAAGGTGCAGCAGCGGCAGCACCAGCTCCTGCGCCTCAAGCGGCAGCACCAGTTGCTGCGGCTCCAGCGGCCGC
TGCACTGAAAGAAGTGCAAGTACCAGACATTGGCGGTGATGAAGTTGAAGTGACTGAAATCATGGTCAAAGTGGGCGATG
TGGTTGCGGAAGAGCAATCTCTGATCACCGTTGAAGGCGACAAAGCGTCAATGGAAGTACCTGCACCTTTCGCTGGTACG
GTTAAAGAGATCAAAATCGCTGCGGGCGACAAAGTATCAACAGGTTCACTGATCATGGTGTTTGAAGTAGCCGGTGCTGC
GCCAGTTGCAGCACCAGTTCAAGCTGCGGCACCAGCGGCAGCTGCTGCTCCAGCTGTTGCAGCACTGAAAGAAGTTCAAG
TACCTGATATCGGCGGTGACGAAGTGACCGTAACGGAAATCATGGTTAACGTGGGCGACAGCATCAGCGAAGAGCAATCT
CTGATCACCGTAGAAGGCGATAAGGCTTCTATGGAAGTACCAGCACCTTTTGCTGGAACTCTCAAAGAGATCAAAGTGGC
TGCAGGCGATAAAGTGAAAACAGGTTCGCTGATCATGGTGTTTGAAGTTGCTGGCGCAGCGCCAGTCGCGGCACCAGTTC
AAGCAGCACCAGCACCAGCACCTGCGGCAGCGCCTGCTCAAGCAGCAGCTCCTGCGGCGGCTGCACCTGCAACAAGTGGT
GAATTCCAAGAAAACCATGAGTACTCACACGCTTCTCCAGTCGTGCGTCGTCTGGCTCGTGAATTTGGTGTTAACCTAGC
CAAAGTCAAAGGCTCTGGCCGCAAGAACCGTATCCTGAAAGAAGACGTACAGAACTACGTGAAAGAAGCGCTGAAACGTC
TGGAATCTGGCGCGCAAGCGGCGGCTTCAGGTAAAGGTGATGGCGCAGCGCTGGGCTTACTGCCTTGGCCAAAAGTGGAC
TTCAGCAAGTTCGGTGATACCGAAGTGCAACCACTGTCTCGCATCAAGAAAATCTCGGGTGCTAACCTGCACCGTAACTG
GGTGATGATCCCGCACGTGACCCAATGGGACAATGCAGACATCACTGAGCTAGAGAAATTCCGTCAAGAGCAAAACGCGA
TGGAAGCGAAGCGCGACACTGGCATGAAGATCACTCCGCTGGTGTTCATCATGAAAGCCGCTGCGAAAGCGCTGGAAGCT
TTCCCTGCGTTCAACTCTTCTCTGTCTGACGATGGCGAAAGCTTGATCCTGAAGAAATACGTGAACATCGGTATTGCAGT
GGATACACCAAATGGTCTGGTGGTTCCTGTGTTTAAAGATGTGAACAAGAAAGGCATCTACGAGCTGTCAAAAGAGCTGG
CTGAAGTCTCGAAGAAAGCCCGTGGCGGTAAGCTGACTGCCGCTGACATGCAAGGTGGCTGTTTCACTATCTCTAGCCTA
GGCGGTATTGGCGGTACAGCGTTTACCCCAATCGTTAACGCACCTGAAGTGGCTATTTTGGGTGTGTCTAAGTCTGAGAT
GAAGCCAGTGTGGAACGGCAAAGAGTTCGCGCCACGTCTGCAACTGCCACTGTCACTGTCTTACGATCACCGCGTGATCG
ACGGTGCAGAAGGTGCACGCTTCATTACTTACCTGAACGAGTGTCTAAGCGACATCCGTCGTCTGGTACTGTAA

Upstream 100 bases:

>100_bases
TGAACTGGCAAAACGTGGTGAAGTTGAGAAGTCTGTGGTTGCAGCAGCAATCAAGAAATTCGACATCGATACTGAAAAAA
CCAACCCGCTGTACGCTTAA

Downstream 100 bases:

>100_bases
GCGCATTCTCGGGTGGCGAAAGCCACCCGATTTAATGGTTATCTCCCAATTTGACGTTGAAACTGATTTTCCGTTGAATT
GTTGTCTAGCTCACAGGCTA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MKVGKVMAIEIYVPDIGADEVEVTEILVKVGDKVAEEQSLITVEGDKASMEVPASQAGIVKEIKVVAGDKVSTGSLIMVF
EAEGAAAAAPAPAPQAAAPVAAAPAAAALKEVQVPDIGGDEVEVTEIMVKVGDVVAEEQSLITVEGDKASMEVPAPFAGT
VKEIKIAAGDKVSTGSLIMVFEVAGAAPVAAPVQAAAPAAAAAPAVAALKEVQVPDIGGDEVTVTEIMVNVGDSISEEQS
LITVEGDKASMEVPAPFAGTLKEIKVAAGDKVKTGSLIMVFEVAGAAPVAAPVQAAPAPAPAAAPAQAAAPAAAAPATSG
EFQENHEYSHASPVVRRLAREFGVNLAKVKGSGRKNRILKEDVQNYVKEALKRLESGAQAAASGKGDGAALGLLPWPKVD
FSKFGDTEVQPLSRIKKISGANLHRNWVMIPHVTQWDNADITELEKFRQEQNAMEAKRDTGMKITPLVFIMKAAAKALEA
FPAFNSSLSDDGESLILKKYVNIGIAVDTPNGLVVPVFKDVNKKGIYELSKELAEVSKKARGGKLTAADMQGGCFTISSL
GGIGGTAFTPIVNAPEVAILGVSKSEMKPVWNGKEFAPRLQLPLSLSYDHRVIDGAEGARFITYLNECLSDIRRLVL

Sequences:

>Translated_637_residues
MKVGKVMAIEIYVPDIGADEVEVTEILVKVGDKVAEEQSLITVEGDKASMEVPASQAGIVKEIKVVAGDKVSTGSLIMVF
EAEGAAAAAPAPAPQAAAPVAAAPAAAALKEVQVPDIGGDEVEVTEIMVKVGDVVAEEQSLITVEGDKASMEVPAPFAGT
VKEIKIAAGDKVSTGSLIMVFEVAGAAPVAAPVQAAAPAAAAAPAVAALKEVQVPDIGGDEVTVTEIMVNVGDSISEEQS
LITVEGDKASMEVPAPFAGTLKEIKVAAGDKVKTGSLIMVFEVAGAAPVAAPVQAAPAPAPAAAPAQAAAPAAAAPATSG
EFQENHEYSHASPVVRRLAREFGVNLAKVKGSGRKNRILKEDVQNYVKEALKRLESGAQAAASGKGDGAALGLLPWPKVD
FSKFGDTEVQPLSRIKKISGANLHRNWVMIPHVTQWDNADITELEKFRQEQNAMEAKRDTGMKITPLVFIMKAAAKALEA
FPAFNSSLSDDGESLILKKYVNIGIAVDTPNGLVVPVFKDVNKKGIYELSKELAEVSKKARGGKLTAADMQGGCFTISSL
GGIGGTAFTPIVNAPEVAILGVSKSEMKPVWNGKEFAPRLQLPLSLSYDHRVIDGAEGARFITYLNECLSDIRRLVL
>Mature_637_residues
MKVGKVMAIEIYVPDIGADEVEVTEILVKVGDKVAEEQSLITVEGDKASMEVPASQAGIVKEIKVVAGDKVSTGSLIMVF
EAEGAAAAAPAPAPQAAAPVAAAPAAAALKEVQVPDIGGDEVEVTEIMVKVGDVVAEEQSLITVEGDKASMEVPAPFAGT
VKEIKIAAGDKVSTGSLIMVFEVAGAAPVAAPVQAAAPAAAAAPAVAALKEVQVPDIGGDEVTVTEIMVNVGDSISEEQS
LITVEGDKASMEVPAPFAGTLKEIKVAAGDKVKTGSLIMVFEVAGAAPVAAPVQAAPAPAPAAAPAQAAAPAAAAPATSG
EFQENHEYSHASPVVRRLAREFGVNLAKVKGSGRKNRILKEDVQNYVKEALKRLESGAQAAASGKGDGAALGLLPWPKVD
FSKFGDTEVQPLSRIKKISGANLHRNWVMIPHVTQWDNADITELEKFRQEQNAMEAKRDTGMKITPLVFIMKAAAKALEA
FPAFNSSLSDDGESLILKKYVNIGIAVDTPNGLVVPVFKDVNKKGIYELSKELAEVSKKARGGKLTAADMQGGCFTISSL
GGIGGTAFTPIVNAPEVAILGVSKSEMKPVWNGKEFAPRLQLPLSLSYDHRVIDGAEGARFITYLNECLSDIRRLVL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=433, Percent_Identity=31.8706697459584, Blast_Score=180, Evalue=4e-45,
Organism=Homo sapiens, GI31711992, Length=429, Percent_Identity=29.8368298368298, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI19923748, Length=229, Percent_Identity=31.4410480349345, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI203098816, Length=444, Percent_Identity=27.7027027027027, Blast_Score=114, Evalue=4e-25,
Organism=Homo sapiens, GI203098753, Length=456, Percent_Identity=27.6315789473684, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=35.2564102564103, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1786305, Length=635, Percent_Identity=71.8110236220472, Blast_Score=803, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=408, Percent_Identity=30.1470588235294, Blast_Score=165, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=30.4761904761905, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=444, Percent_Identity=31.3063063063063, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=217, Percent_Identity=35.9447004608295, Blast_Score=129, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=314, Percent_Identity=30.2547770700637, Blast_Score=115, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=419, Percent_Identity=29.1169451073986, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6324258, Length=440, Percent_Identity=29.0909090909091, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI18859875, Length=423, Percent_Identity=30.7328605200946, Blast_Score=172, Evalue=8e-43,
Organism=Drosophila melanogaster, GI24645909, Length=190, Percent_Identity=36.3157894736842, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=30.1724137931034, Blast_Score=113, Evalue=5e-25,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=30.1724137931034, Blast_Score=112, Evalue=1e-24,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 66197; Mature: 66197

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGKVMAIEIYVPDIGADEVEVTEILVKVGDKVAEEQSLITVEGDKASMEVPASQAGIV
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCHH
KEIKVVAGDKVSTGSLIMVFEAEGAAAAAPAPAPQAAAPVAAAPAAAALKEVQVPDIGGD
HHEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHCCHHHHHHHCCCCCCCCC
EVEVTEIMVKVGDVVAEEQSLITVEGDKASMEVPAPFAGTVKEIKIAAGDKVSTGSLIMV
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCCCEEEEEECCCCCCCCCEEEE
FEVAGAAPVAAPVQAAAPAAAAAPAVAALKEVQVPDIGGDEVTVTEIMVNVGDSISEEQS
EEECCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHCCCCCCCCCC
LITVEGDKASMEVPAPFAGTLKEIKVAAGDKVKTGSLIMVFEVAGAAPVAAPVQAAPAPA
EEEEECCCCCEECCCCCHHHHHHHEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
PAAAPAQAAAPAAAAPATSGEFQENHEYSHASPVVRRLAREFGVNLAKVKGSGRKNRILK
CCCCCCHHCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHH
EDVQNYVKEALKRLESGAQAAASGKGDGAALGLLPWPKVDFSKFGDTEVQPLSRIKKISG
HHHHHHHHHHHHHHHCCCHHHCCCCCCCEEECCCCCCCCCHHHCCCCCHHHHHHHHHHCC
ANLHRNWVMIPHVTQWDNADITELEKFRQEQNAMEAKRDTGMKITPLVFIMKAAAKALEA
CCEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHH
FPAFNSSLSDDGESLILKKYVNIGIAVDTPNGLVVPVFKDVNKKGIYELSKELAEVSKKA
HHCCCCCCCCCCHHHHHEECCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHC
RGGKLTAADMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSEMKPVWNGKEFAPRL
CCCCEEEECCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCC
QLPLSLSYDHRVIDGAEGARFITYLNECLSDIRRLVL
CCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKVGKVMAIEIYVPDIGADEVEVTEILVKVGDKVAEEQSLITVEGDKASMEVPASQAGIV
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCHH
KEIKVVAGDKVSTGSLIMVFEAEGAAAAAPAPAPQAAAPVAAAPAAAALKEVQVPDIGGD
HHEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHCCHHHHHHHCCCCCCCCC
EVEVTEIMVKVGDVVAEEQSLITVEGDKASMEVPAPFAGTVKEIKIAAGDKVSTGSLIMV
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCCCEEEEEECCCCCCCCCEEEE
FEVAGAAPVAAPVQAAAPAAAAAPAVAALKEVQVPDIGGDEVTVTEIMVNVGDSISEEQS
EEECCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHCCCCCCCCCC
LITVEGDKASMEVPAPFAGTLKEIKVAAGDKVKTGSLIMVFEVAGAAPVAAPVQAAPAPA
EEEEECCCCCEECCCCCHHHHHHHEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
PAAAPAQAAAPAAAAPATSGEFQENHEYSHASPVVRRLAREFGVNLAKVKGSGRKNRILK
CCCCCCHHCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHH
EDVQNYVKEALKRLESGAQAAASGKGDGAALGLLPWPKVDFSKFGDTEVQPLSRIKKISG
HHHHHHHHHHHHHHHCCCHHHCCCCCCCEEECCCCCCCCCHHHCCCCCHHHHHHHHHHCC
ANLHRNWVMIPHVTQWDNADITELEKFRQEQNAMEAKRDTGMKITPLVFIMKAAAKALEA
CCEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHH
FPAFNSSLSDDGESLILKKYVNIGIAVDTPNGLVVPVFKDVNKKGIYELSKELAEVSKKA
HHCCCCCCCCCCHHHHHEECCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHC
RGGKLTAADMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSEMKPVWNGKEFAPRL
CCCCEEEECCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCC
QLPLSLSYDHRVIDGAEGARFITYLNECLSDIRRLVL
CCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]