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The map label for this gene is nqrB

Identifier: 147674588

GI number: 147674588

Start: 2001521

End: 2002768

Strand: Reverse

Name: nqrB

Synonym: VC0395_A1883

Alternate gene names: 147674588

Gene position: 2002768-2001521 (Counterclockwise)

Preceding gene: 147674300

Following gene: 147673911

Centisome position: 66.23

GC content: 49.92

Gene sequence:

>1248_bases
ATGGGCCTTAAAAAGTTTCTTGAAGACATCGAGCATCATTTTGAGCCAGGCGGCAAACACGAGAAGTGGTTTGCCCTGTA
TGAAGCAGCGGCGACGCTGTTCTATACACCAGGTCTGGTAACCAAAAGAAGCTCGCACGTTCGTGATAGCGTTGACCTAA
AACGTATCATGATCATGGTTTGGCTTGCCGTATTCCCAGCGATGTTCTGGGGTATGTACAACGCTGGCGGCCAAGCTATT
GCAGCACTTAACCATCTGTATTCAGGTGATCAACTCGCGGCGATTGTGGCGGGTAACTGGCACTACTGGCTAACCGAAAT
GCTTGGCGGAACCATGTCAAGCGATGCTGGTTGGGGCAGTAAAATGCTGTTGGGTGCAACCTACTTCCTACCTATCTACG
CTACGGTGTTCATCGTCGGTGGTTTCTGGGAAGTGTTGTTCTGTATGGTGCGCAAGCATGAAGTCAACGAAGGTTTCTTC
GTTACCTCTATTCTGTTTGCTCTGATTGTTCCGCCAACTCTGCCTCTGTGGCAAGCTGCACTGGGTATTACCTTTGGTGT
TGTGGTTGCCAAAGAAGTGTTTGGTGGTACAGGCCGTAACTTCCTTAACCCAGCGCTGGCTGGCCGTGCTTTCCTGTTCT
TTGCTTACCCAGCACAGATCTCAGGTGACCTAGTATGGACTGCGGCAGATGGCTACTCAGGCGCAACTGCGCTGAGCCAA
TGGGCACAAGGTGGTGCAGGTGCACTGATTAACAACGCGACTGGTCAAACGATCACTTGGATGGATGCTTTCATCGGTAA
CATCCCAGGTTCAATTGGTGAAGTGTCTACTCTGGCACTCATGATTGGTGCAGCGTTCATTGTTTATATGGGCATTGCTT
CATGGCGCATTATTGGCGGCGTGATGATTGGTATGATCCTGCTGTCGACACTGTTTAACGTGATTGGCTCAGATACCAAC
GCAATGTTCAACATGCCTTGGCATTGGCACCTAGTATTGGGTGGTTTCGCATTCGGTATGTTCTTCATGGCGACTGACCC
AGTTTCTGCGTCCTTCACCAATAGCGGTAAATGGGCTTACGGTATTCTGATCGGTGTTATGTGTGTGCTGATCCGTGTTG
TGAACCCGGCTTACCCAGAAGGCATGATGCTGGCGATCCTATTCGCGAACCTATTTGCGCCACTGTTTGACCATGTGGTT
GTAGAGAGAAATATCAAGCGGAGATTAGCGCGCTATGGCAAGCAATAA

Upstream 100 bases:

>100_bases
AAGATCTAGCACTGTGTACGTTCGTTTGCCCAGGCAAATACGAGTACGGTCAGCTCCTGCGTGAGTGCTTAGACAAGATC
GAGAAAGAAGGGTAATTTCC

Downstream 100 bases:

>100_bases
CGATAGCATTAAAAAGACGCTGTTTGTTGTTATCGCGTTGAGCCTAGTGTGCTCAATCATTGTATCGGCAGCGGCTGTCG
GTCTGCGTGACAAGCAAAAA

Product: Na(+)-translocating NADH-quinone reductase subunit B

Products: NA

Alternate protein names: Na(+)-NQR subunit B; Na(+)-translocating NQR subunit B; NQR complex subunit B; NQR-1 subunit B

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MGLKKFLEDIEHHFEPGGKHEKWFALYEAAATLFYTPGLVTKRSSHVRDSVDLKRIMIMVWLAVFPAMFWGMYNAGGQAI
AALNHLYSGDQLAAIVAGNWHYWLTEMLGGTMSSDAGWGSKMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFF
VTSILFALIVPPTLPLWQAALGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAYPAQISGDLVWTAADGYSGATALSQ
WAQGGAGALINNATGQTITWMDAFIGNIPGSIGEVSTLALMIGAAFIVYMGIASWRIIGGVMIGMILLSTLFNVIGSDTN
AMFNMPWHWHLVLGGFAFGMFFMATDPVSASFTNSGKWAYGILIGVMCVLIRVVNPAYPEGMMLAILFANLFAPLFDHVV
VERNIKRRLARYGKQ

Sequences:

>Translated_415_residues
MGLKKFLEDIEHHFEPGGKHEKWFALYEAAATLFYTPGLVTKRSSHVRDSVDLKRIMIMVWLAVFPAMFWGMYNAGGQAI
AALNHLYSGDQLAAIVAGNWHYWLTEMLGGTMSSDAGWGSKMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFF
VTSILFALIVPPTLPLWQAALGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAYPAQISGDLVWTAADGYSGATALSQ
WAQGGAGALINNATGQTITWMDAFIGNIPGSIGEVSTLALMIGAAFIVYMGIASWRIIGGVMIGMILLSTLFNVIGSDTN
AMFNMPWHWHLVLGGFAFGMFFMATDPVSASFTNSGKWAYGILIGVMCVLIRVVNPAYPEGMMLAILFANLFAPLFDHVV
VERNIKRRLARYGKQ
>Mature_414_residues
GLKKFLEDIEHHFEPGGKHEKWFALYEAAATLFYTPGLVTKRSSHVRDSVDLKRIMIMVWLAVFPAMFWGMYNAGGQAIA
ALNHLYSGDQLAAIVAGNWHYWLTEMLGGTMSSDAGWGSKMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFFV
TSILFALIVPPTLPLWQAALGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAYPAQISGDLVWTAADGYSGATALSQW
AQGGAGALINNATGQTITWMDAFIGNIPGSIGEVSTLALMIGAAFIVYMGIASWRIIGGVMIGMILLSTLFNVIGSDTNA
MFNMPWHWHLVLGGFAFGMFFMATDPVSASFTNSGKWAYGILIGVMCVLIRVVNPAYPEGMMLAILFANLFAPLFDHVVV
ERNIKRRLARYGKQ

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. NqrA to nqrE are probably involved in the second step, the conversion of ubisemiqui

COG id: COG1805

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrB/rnfD family

Homologues:

Organism=Escherichia coli, GI1787917, Length=302, Percent_Identity=27.1523178807947, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NQRB_VIBCH (Q9KPS2)

Other databases:

- EMBL:   AF117331
- EMBL:   AE003852
- PIR:   F82094
- RefSeq:   NP_231925.1
- GeneID:   2613216
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2294
- TIGR:   VC_2294
- HOGENOM:   HBG454412
- OMA:   TVFYTPG
- ProtClustDB:   PRK05349
- HAMAP:   MF_00426
- InterPro:   IPR010966
- InterPro:   IPR004338
- PIRSF:   PIRSF016055
- TIGRFAMs:   TIGR01937

Pfam domain/function: PF03116 NQR2_RnfD_RnfE

EC number: NA

Molecular weight: Translated: 45358; Mature: 45227

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1aa11bb8)-; HASH(0x17134b7c)-; HASH(0x1a4a2ee0)-; HASH(0x1b006a28)-; HASH(0x1b0c1404)-; HASH(0x17034108)-; HASH(0x1af04148)-; HASH(0x183c5100)-; HASH(0x1a9bab2c)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKKFLEDIEHHFEPGGKHEKWFALYEAAATLFYTPGLVTKRSSHVRDSVDLKRIMIMV
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
WLAVFPAMFWGMYNAGGQAIAALNHLYSGDQLAAIVAGNWHYWLTEMLGGTMSSDAGWGS
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCC
KMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFFVTSILFALIVPPTLPLWQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHH
LGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAYPAQISGDLVWTAADGYSGATALSQ
HHHHHHHHHHHHHHCCCCCHHCCHHHHCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHH
WAQGGAGALINNATGQTITWMDAFIGNIPGSIGEVSTLALMIGAAFIVYMGIASWRIIGG
HHCCCCCCEEECCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMIGMILLSTLFNVIGSDTNAMFNMPWHWHLVLGGFAFGMFFMATDPVSASFTNSGKWAY
HHHHHHHHHHHHHHHCCCCCCEEECCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
GILIGVMCVLIRVVNPAYPEGMMLAILFANLFAPLFDHVVVERNIKRRLARYGKQ
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
GLKKFLEDIEHHFEPGGKHEKWFALYEAAATLFYTPGLVTKRSSHVRDSVDLKRIMIMV
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
WLAVFPAMFWGMYNAGGQAIAALNHLYSGDQLAAIVAGNWHYWLTEMLGGTMSSDAGWGS
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCC
KMLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFFVTSILFALIVPPTLPLWQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHH
LGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAYPAQISGDLVWTAADGYSGATALSQ
HHHHHHHHHHHHHHCCCCCHHCCHHHHCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHH
WAQGGAGALINNATGQTITWMDAFIGNIPGSIGEVSTLALMIGAAFIVYMGIASWRIIGG
HHCCCCCCEEECCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMIGMILLSTLFNVIGSDTNAMFNMPWHWHLVLGGFAFGMFFMATDPVSASFTNSGKWAY
HHHHHHHHHHHHHHHCCCCCCEEECCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
GILIGVMCVLIRVVNPAYPEGMMLAILFANLFAPLFDHVVVERNIKRRLARYGKQ
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10077658; 10952301