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The map label for this gene is lpdA [H]

Identifier: 147674273

GI number: 147674273

Start: 2134524

End: 2135951

Strand: Reverse

Name: lpdA [H]

Synonym: VC0395_A1988

Alternate gene names: 147674273

Gene position: 2135951-2134524 (Counterclockwise)

Preceding gene: 147674758

Following gene: 147675217

Centisome position: 70.63

GC content: 49.09

Gene sequence:

>1428_bases
ATGAGCAAAGAAATTAAAGCCCAAGTCGTGGTGCTTGGTGCCGGTCCTGCTGGTTACTCCGCCGCATTCCGCTGTGCAGA
CTTAGGTCTGGATACCGTCATCATCGAACGTTACAACACGCTAGGTGGTGTGTGTTTGAACGTTGGTTGTATTCCTTCTA
AAGCACTGCTGCACGTGGCAAAAGTGATTGAAGAAGCCAAAGCGCTGACTGAGCACGGTATCGTGTTTGGCGAGCCAAAG
ACAGACATCGACAAAGTTCGTCTGTGGAAAGAAAAAGTGATCAATCAGCTGACCGGTGGTCTGGCTGGTATGGCAAAAAT
GCGTAAAGTGAACGTAGTGAACGGCTACGGTAAATTCACCGGCCCGAACACTATTGAAGTGGAAGGCGAAGAGGGCAAAA
CTGTCGTAACTTTCGATAACGCGATCGTTGCTGCAGGTTCTCGTCCAATCAAACTGCCATTCATTCCGCATGAAGACCCA
CGTATTTGGGATTCAACTGATGCGCTGGAACTGAAAGAAGTGCCTGGAAAACTGCTGATCATGGGCGGCGGTATCATCGG
CCTGGAAATGGCGACCGTTTACCATTCACTGGGTTCTAAAATTGATGTAGTTGAGATGTTTGACCAACTTATCCCTGCTG
CGGATAAAGACATGGTTAAAGTCTATACCAAACGCATCAAAGATAAGTTCAACCTGATGCTGGAAACCAAAGTTACCGCC
GTTGAAGCGAAAGAAGATGGTATCTACGTTTCAATGGAAGGTAAGAGCGCACCAGCACAAGCTGAGCGTTACGATGCGGT
TCTGGTTGCAATTGGCCGTGTACCAAACGGTAAACTACTGGATGCTGAGAAAGCGGGTCTGGAAGTGGACGAGCGTGGCT
TTATCCGCGTGGATAAGCAAATGCGCACTAACGTTCCGCACATCTTCGCGATCGGTGACATCGTGGGTCAACCAATGCTA
GCACATAAAGGCGTGCATGAAGGCCACGTAGCGGCAGAAGTGATCTCTGGTAAGAAGCACTACTTCGATCCAAAAGTGAT
CCCATCCATCGCTTACACTGAGCCAGAAGTGGCTTGGGTTGGTAAGACAGAAAAAGAAGCAAAAGCAGAAGGCATCAACT
ATGAAGTTGCTACTTTCCCATGGGCAGCCTCTGGCCGTGCGATTGCTTCTGACTGTGCAGACGGTATGACTAAGCTTATC
TTCGATAAAGAGACTCATCGTGTGATCGGTGGTGCTATCGTAGGTACTAACGGTGGTGAACTGTTGGGTGAAATCGGTCT
GGCGATAGAGATGGGCTGTGATGCAGAAGACATCGCACTGACCATTCATGCTCACCCAACGCTGCATGAGTCTGTGGGTC
TGGCCGCTGAAGTCTTCGAAGGCACCATCACTGACCTGCCAAACGCCAAAGCGAAAAAGAAAAAATAA

Upstream 100 bases:

>100_bases
ATGCTTGATTTGAAACATTCGTTTTTGATTCGTTTGTAAATAAAACAACTAGATAGAACACCCATCAGCCTGTTAGGGAT
ATTGACTACAAGAGGTCGAA

Downstream 100 bases:

>100_bases
TTTCTGATTCACTGGTTTGGTTAAACAGTGTTTGATTGAAGAAACCGCTGCTTGCAGCGGTTTTTTTCGTCTTTGATTCC
CAATTGAATGCAAAGTAAGC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSKEIKAQVVVLGAGPAGYSAAFRCADLGLDTVIIERYNTLGGVCLNVGCIPSKALLHVAKVIEEAKALTEHGIVFGEPK
TDIDKVRLWKEKVINQLTGGLAGMAKMRKVNVVNGYGKFTGPNTIEVEGEEGKTVVTFDNAIVAAGSRPIKLPFIPHEDP
RIWDSTDALELKEVPGKLLIMGGGIIGLEMATVYHSLGSKIDVVEMFDQLIPAADKDMVKVYTKRIKDKFNLMLETKVTA
VEAKEDGIYVSMEGKSAPAQAERYDAVLVAIGRVPNGKLLDAEKAGLEVDERGFIRVDKQMRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVGKTEKEAKAEGINYEVATFPWAASGRAIASDCADGMTKLI
FDKETHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGTITDLPNAKAKKKK

Sequences:

>Translated_475_residues
MSKEIKAQVVVLGAGPAGYSAAFRCADLGLDTVIIERYNTLGGVCLNVGCIPSKALLHVAKVIEEAKALTEHGIVFGEPK
TDIDKVRLWKEKVINQLTGGLAGMAKMRKVNVVNGYGKFTGPNTIEVEGEEGKTVVTFDNAIVAAGSRPIKLPFIPHEDP
RIWDSTDALELKEVPGKLLIMGGGIIGLEMATVYHSLGSKIDVVEMFDQLIPAADKDMVKVYTKRIKDKFNLMLETKVTA
VEAKEDGIYVSMEGKSAPAQAERYDAVLVAIGRVPNGKLLDAEKAGLEVDERGFIRVDKQMRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVGKTEKEAKAEGINYEVATFPWAASGRAIASDCADGMTKLI
FDKETHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGTITDLPNAKAKKKK
>Mature_474_residues
SKEIKAQVVVLGAGPAGYSAAFRCADLGLDTVIIERYNTLGGVCLNVGCIPSKALLHVAKVIEEAKALTEHGIVFGEPKT
DIDKVRLWKEKVINQLTGGLAGMAKMRKVNVVNGYGKFTGPNTIEVEGEEGKTVVTFDNAIVAAGSRPIKLPFIPHEDPR
IWDSTDALELKEVPGKLLIMGGGIIGLEMATVYHSLGSKIDVVEMFDQLIPAADKDMVKVYTKRIKDKFNLMLETKVTAV
EAKEDGIYVSMEGKSAPAQAERYDAVLVAIGRVPNGKLLDAEKAGLEVDERGFIRVDKQMRTNVPHIFAIGDIVGQPMLA
HKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVGKTEKEAKAEGINYEVATFPWAASGRAIASDCADGMTKLIF
DKETHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGTITDLPNAKAKKKK

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=456, Percent_Identity=43.2017543859649, Blast_Score=342, Evalue=6e-94,
Organism=Homo sapiens, GI50301238, Length=451, Percent_Identity=27.2727272727273, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI148277071, Length=436, Percent_Identity=29.3577981651376, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI33519430, Length=436, Percent_Identity=29.3577981651376, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI33519428, Length=436, Percent_Identity=29.3577981651376, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI33519426, Length=436, Percent_Identity=29.3577981651376, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI148277065, Length=436, Percent_Identity=29.3577981651376, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI22035672, Length=433, Percent_Identity=29.0993071593533, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI291045266, Length=436, Percent_Identity=29.1284403669725, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI291045268, Length=427, Percent_Identity=28.1030444964871, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=90.084388185654, Blast_Score=876, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=29.5503211991435, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=29.4505494505495, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1789915, Length=444, Percent_Identity=27.9279279279279, Blast_Score=141, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=454, Percent_Identity=38.1057268722467, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=28.3613445378151, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=25.6880733944954, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=25.6880733944954, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=439, Percent_Identity=27.5626423690205, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=41.2280701754386, Blast_Score=311, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=27.5641025641026, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6325166, Length=455, Percent_Identity=26.8131868131868, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21358499, Length=453, Percent_Identity=40.3973509933775, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24640553, Length=462, Percent_Identity=28.3549783549784, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640549, Length=462, Percent_Identity=28.3549783549784, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640551, Length=462, Percent_Identity=28.3549783549784, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17737741, Length=382, Percent_Identity=28.2722513089005, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51010; Mature: 50878

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEIKAQVVVLGAGPAGYSAAFRCADLGLDTVIIERYNTLGGVCLNVGCIPSKALLHVA
CCCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEHHCCCCCEEEEECCCCHHHHHHHH
KVIEEAKALTEHGIVFGEPKTDIDKVRLWKEKVINQLTGGLAGMAKMRKVNVVNGYGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEEEEEEECCCCCC
GPNTIEVEGEEGKTVVTFDNAIVAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPGKLLI
CCCEEEEECCCCCEEEEECCEEEECCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEEE
MGGGIIGLEMATVYHSLGSKIDVVEMFDQLIPAADKDMVKVYTKRIKDKFNLMLETKVTA
EECCEEEHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVSMEGKSAPAQAERYDAVLVAIGRVPNGKLLDAEKAGLEVDERGFIRVDKQ
EEECCCCEEEEECCCCCCCHHHHCCEEEEEEECCCCCCEECHHHCCCCCCCCCCEEECHH
MRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEE
GKTEKEAKAEGINYEVATFPWAASGRAIASDCADGMTKLIFDKETHRVIGGAIVGTNGGE
CCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHEEECEEEEECCCHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGTITDLPNAKAKKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCHHHHHHCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKEIKAQVVVLGAGPAGYSAAFRCADLGLDTVIIERYNTLGGVCLNVGCIPSKALLHVA
CCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEHHCCCCCEEEEECCCCHHHHHHHH
KVIEEAKALTEHGIVFGEPKTDIDKVRLWKEKVINQLTGGLAGMAKMRKVNVVNGYGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEEEEEEECCCCCC
GPNTIEVEGEEGKTVVTFDNAIVAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPGKLLI
CCCEEEEECCCCCEEEEECCEEEECCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEEE
MGGGIIGLEMATVYHSLGSKIDVVEMFDQLIPAADKDMVKVYTKRIKDKFNLMLETKVTA
EECCEEEHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVSMEGKSAPAQAERYDAVLVAIGRVPNGKLLDAEKAGLEVDERGFIRVDKQ
EEECCCCEEEEECCCCCCCHHHHCCEEEEEEECCCCCCEECHHHCCCCCCCCCCEEECHH
MRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEE
GKTEKEAKAEGINYEVATFPWAASGRAIASDCADGMTKLIFDKETHRVIGGAIVGTNGGE
CCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHEEECEEEEECCCHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGTITDLPNAKAKKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCHHHHHHCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]