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The map label for this gene is prfB [H]

Identifier: 147673826

GI number: 147673826

Start: 200437

End: 201336

Strand: Direct

Name: prfB [H]

Synonym: VC0395_A0194

Alternate gene names: 147673826

Gene position: 200437-201336 (Clockwise)

Preceding gene: 147673070

Following gene: 147674731

Centisome position: 6.63

GC content: 48.78

Gene sequence:

>900_bases
GTGGATACCATCGATCAACTGGATCGTGGTGTCGATGATGTCGAAGGTCTACTAGAACTGGCCATCGAAGCGGAAGATCA
AGAAACGTTTGATGAAATTGGTCCTGAATTGGATGAGCTAGAAGCGAAGCTTGAACAGCTCGAATTCCGCCGCATGTTCT
CTGGCGATCACGATTCTTCCGATTGCTACCTTGACCTACAAGCCGGCTCTGGCGGTACAGAAGCGCAAGATTGGACGTCG
ATGCTGCTGCGCATGTACTTGCGCTGGGCAGATGCTAAAGGCTTTAAAACCGAAGTGATTGAAGTGTCGGAAGGCGACGT
AGCAGGATTGAAATCTGCGACAGTGCGTATCATTGGTGATTACGCTTATGGTTGGCTACGTACTGAAACCGGTGTACACC
GTCTAGTGCGTAAATCACCGTTCGATTCAGGTGGCCGCCGTCATACGTCGTTTGCTTCGGCGTTTATCTACCCTGAAGTA
GATGAAAACATAGATATCGAAATTAACCCTGCCGATCTGCGTATAGACGTGTATCGTGCTTCTGGTGCCGGTGGTCAGCA
CGTTAACACCACCGAATCGGCGGTACGTATCACTCACGTACCGACCAATACGGTCGTGCAGTGTCAAAACGATCGCTCAC
AGCACAAAAACAAAGACCAAGCGATGAAGCAACTTCGCGCCAAACTGTTTGAATTGGAGCTGCAAAAGCAGAACGCCGAG
AAACAGGCTAACGAAGATGCTAAATCCGACATTGGTTGGGGTAGCCAAATCCGTTCTTACGTGTTGGATGATTCACGAAT
CAAGGATTTACGTACAGGTATTGAAAACCGCAACACGCAAGCGGTACTCGATGGTGACTTAGACAAATTTATCGAAGCTA
GCCTGAAATCAGGCCTGTAA

Upstream 100 bases:

>100_bases
TCTTGAAGAAGTCAATGCCGAATTAGAACAACCTGATGTCTGGACCCAACCTGAGCGTGCGCAAGCCCTCGGTAAAGAGC
GTTCAGCACTTGAAGCGGTT

Downstream 100 bases:

>100_bases
GCTTTTTACCGACAAACAAGGTACATGCAAATGACTGATGCTGTTCAAAACGAGATTAATCAAGAGCAAATCGCGCAAGA
AGAAAACAAACTGATTGCAG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDTIDQLDRGVDDVEGLLELAIEAEDQETFDEIGPELDELEAKLEQLEFRRMFSGDHDSSDCYLDLQAGSGGTEAQDWTS
MLLRMYLRWADAKGFKTEVIEVSEGDVAGLKSATVRIIGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFASAFIYPEV
DENIDIEINPADLRIDVYRASGAGGQHVNTTESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELELQKQNAE
KQANEDAKSDIGWGSQIRSYVLDDSRIKDLRTGIENRNTQAVLDGDLDKFIEASLKSGL

Sequences:

>Translated_299_residues
MDTIDQLDRGVDDVEGLLELAIEAEDQETFDEIGPELDELEAKLEQLEFRRMFSGDHDSSDCYLDLQAGSGGTEAQDWTS
MLLRMYLRWADAKGFKTEVIEVSEGDVAGLKSATVRIIGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFASAFIYPEV
DENIDIEINPADLRIDVYRASGAGGQHVNTTESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELELQKQNAE
KQANEDAKSDIGWGSQIRSYVLDDSRIKDLRTGIENRNTQAVLDGDLDKFIEASLKSGL
>Mature_299_residues
MDTIDQLDRGVDDVEGLLELAIEAEDQETFDEIGPELDELEAKLEQLEFRRMFSGDHDSSDCYLDLQAGSGGTEAQDWTS
MLLRMYLRWADAKGFKTEVIEVSEGDVAGLKSATVRIIGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFASAFIYPEV
DENIDIEINPADLRIDVYRASGAGGQHVNTTESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELELQKQNAE
KQANEDAKSDIGWGSQIRSYVLDDSRIKDLRTGIENRNTQAVLDGDLDKFIEASLKSGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=243, Percent_Identity=40.7407407407407, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI34577120, Length=311, Percent_Identity=33.1189710610932, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI166795305, Length=150, Percent_Identity=46, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI2367172, Length=299, Percent_Identity=80.9364548494983, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI1787462, Length=240, Percent_Identity=41.25, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=257, Percent_Identity=34.2412451361868, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321295, Length=310, Percent_Identity=34.5161290322581, Blast_Score=154, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19921226, Length=220, Percent_Identity=40, Blast_Score=157, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 33533; Mature: 33533

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTIDQLDRGVDDVEGLLELAIEAEDQETFDEIGPELDELEAKLEQLEFRRMFSGDHDSS
CCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
DCYLDLQAGSGGTEAQDWTSMLLRMYLRWADAKGFKTEVIEVSEGDVAGLKSATVRIIGD
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEEEC
YAYGWLRTETGVHRLVRKSPFDSGGRRHTSFASAFIYPEVDENIDIEINPADLRIDVYRA
HHHHEEECHHHHHHHHHCCCCCCCCCCHHHHHHEEECCCCCCCEEEEECCCEEEEEEEEE
SGAGGQHVNTTESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELELQKQNAE
CCCCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
KQANEDAKSDIGWGSQIRSYVLDDSRIKDLRTGIENRNTQAVLDGDLDKFIEASLKSGL
HHHCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MDTIDQLDRGVDDVEGLLELAIEAEDQETFDEIGPELDELEAKLEQLEFRRMFSGDHDSS
CCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
DCYLDLQAGSGGTEAQDWTSMLLRMYLRWADAKGFKTEVIEVSEGDVAGLKSATVRIIGD
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEEEC
YAYGWLRTETGVHRLVRKSPFDSGGRRHTSFASAFIYPEVDENIDIEINPADLRIDVYRA
HHHHEEECHHHHHHHHHCCCCCCCCCCHHHHHHEEECCCCCCCEEEEECCCEEEEEEEEE
SGAGGQHVNTTESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELELQKQNAE
CCCCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
KQANEDAKSDIGWGSQIRSYVLDDSRIKDLRTGIENRNTQAVLDGDLDKFIEASLKSGL
HHHCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA