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The map label for this gene is wcaJ [H]

Identifier: 146343815

GI number: 146343815

Start: 7376723

End: 7378171

Strand: Reverse

Name: wcaJ [H]

Synonym: BRADO7064

Alternate gene names: 146343815

Gene position: 7378171-7376723 (Counterclockwise)

Preceding gene: 146343816

Following gene: 146343810

Centisome position: 98.95

GC content: 59.77

Gene sequence:

>1449_bases
GTGACCGAGCTATCGGAATCAAGGGCGGAGCGGCGAACAGCGATATATTTCGGCGCAGCGTCGGCAAACGTAGGGCTGCT
CGAGCTCCTTGCAATCGTTTACGAAATGCTGGTGGTGTTCGGCGCAAGCCTGATCGCCGGCTATGCCTATCATATTACCT
ATTACGGCGTATTCCAGAAGCTCGAGAGCTTCGTGGGGATCGGCATCTTCGCGGCCGTTCTTCACATGTTCATTGCTAAA
TCCCAGGGATTGTATGATGCGCCGGTGTTGGCGGGCCTGGACCGGCGTTGGAGCCGGCTGATCTGCGGCTGGCTTCTGGT
CGTCCTGTTCCTGACGCTCATCCTGTTTCTGCTGAAGGCCGGCGCGGAGGTGTCGCGCGGCTCGATGATCGTGTTCGGTT
TCGTCGGCTGCACCGCCTTGGTGGTGGGACGAGCCGTTTTCGAGCGACCTCTCCGCAAGGCGATTGATCGCGGTGTGCTC
GCGACCCGAAGGGCGATCGTTGTCGGATCGCCGGACGAACTGTCTCGTCTGCGCCCGGTGGATCTGTTGAAGAACTATGG
CCTCTCGGAGGTTCATCGAATCTTCTTGTCGGCGAAGCCTGACAGCGACGCTGCAAGTGAAGACGGTGCCGTATCTCAGG
CCGTCATTCAGAGGGCTCGGGAACTGAGGGTCGACGAAATCGTGCTTGCGCTGCGATGGAGTGATGAGGCGCGCATCTAT
CGGATTTGCGAACTGTTCCGCGCCTCTCCGCTGCCGATTCATCTCCTTCCGGATCAGACCGCAGAGCAGTTCCTGTCGCT
GCCGATCCTGGCGACGGGACCGCTTCCAACGGTCGAAATGCAGCGTGCGCCGTTATCCAGACTTGAACGGACCTGCAAGC
GAGCATTCGACATCCTGGCCGCATCGGCTCTGTTGATTGTCTTCTTCCCGGTGATGGTGGCGGCCGCGTTGTCGGTCAAG
CTCGACTCGGCCGGACCGATCATTTTTCGGCAGCGGAGAAACGGGTTCGACGGAAGAACATTCCGGATCTTCAAATTCCG
TACGATGCTCGTCCAGGAGGATGGGCCGAAGGTCGAGCAGGCGCAGCCGAATGACCCGCGTGTCACACGGGTTGGGCGCA
TCCTGCGGCGGCGAAGCATCGACGAATTGCCGCAGCTGCTCAACGTTCTCCGCGGCGAGATGTCGCTTGTCGGGCCAAGG
CCGCATGCCATTGCTCATGATAGCGAGTTTTCGAAGCTCATTGCGAGCTACGCCTACCGGCAACATGTCAAACCGGGCAT
CACCGGTTTGGCGCAGGTTCGCGGTCTGCGGGGGCCCACTCCGGCCGTAGAGGACATGCAGAAAAGGGTCGAGCACGATC
TGGCCTACATTTCGAGCTGGAGCTTCACGCTCGATCTACACATCCTGCTGCGGACGGTCCGCGTTTTCTTTCTGAGCAAT
GCTGATTAG

Upstream 100 bases:

>100_bases
GCAGGTCGAGTTTGACCCATTGGCGCCGCTCGGAGCAGCTTGCCTCGTCGCTAACGGACTGATAACCATCCGCTGAAACG
TTAATAGGTGTTAACTCTCC

Downstream 100 bases:

>100_bases
TTGCCGCGTATCAGAGTCTGAATGTCGGTTCCGGCTGCCTTTGTCGTCTGGGCAATGATCCGGCGATTGGCCTCCCCTGC
CGCGAGATATGCGCGACCAA

Product: putative sugar transferase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTELSESRAERRTAIYFGAASANVGLLELLAIVYEMLVVFGASLIAGYAYHITYYGVFQKLESFVGIGIFAAVLHMFIAK
SQGLYDAPVLAGLDRRWSRLICGWLLVVLFLTLILFLLKAGAEVSRGSMIVFGFVGCTALVVGRAVFERPLRKAIDRGVL
ATRRAIVVGSPDELSRLRPVDLLKNYGLSEVHRIFLSAKPDSDAASEDGAVSQAVIQRARELRVDEIVLALRWSDEARIY
RICELFRASPLPIHLLPDQTAEQFLSLPILATGPLPTVEMQRAPLSRLERTCKRAFDILAASALLIVFFPVMVAAALSVK
LDSAGPIIFRQRRNGFDGRTFRIFKFRTMLVQEDGPKVEQAQPNDPRVTRVGRILRRRSIDELPQLLNVLRGEMSLVGPR
PHAIAHDSEFSKLIASYAYRQHVKPGITGLAQVRGLRGPTPAVEDMQKRVEHDLAYISSWSFTLDLHILLRTVRVFFLSN
AD

Sequences:

>Translated_482_residues
MTELSESRAERRTAIYFGAASANVGLLELLAIVYEMLVVFGASLIAGYAYHITYYGVFQKLESFVGIGIFAAVLHMFIAK
SQGLYDAPVLAGLDRRWSRLICGWLLVVLFLTLILFLLKAGAEVSRGSMIVFGFVGCTALVVGRAVFERPLRKAIDRGVL
ATRRAIVVGSPDELSRLRPVDLLKNYGLSEVHRIFLSAKPDSDAASEDGAVSQAVIQRARELRVDEIVLALRWSDEARIY
RICELFRASPLPIHLLPDQTAEQFLSLPILATGPLPTVEMQRAPLSRLERTCKRAFDILAASALLIVFFPVMVAAALSVK
LDSAGPIIFRQRRNGFDGRTFRIFKFRTMLVQEDGPKVEQAQPNDPRVTRVGRILRRRSIDELPQLLNVLRGEMSLVGPR
PHAIAHDSEFSKLIASYAYRQHVKPGITGLAQVRGLRGPTPAVEDMQKRVEHDLAYISSWSFTLDLHILLRTVRVFFLSN
AD
>Mature_481_residues
TELSESRAERRTAIYFGAASANVGLLELLAIVYEMLVVFGASLIAGYAYHITYYGVFQKLESFVGIGIFAAVLHMFIAKS
QGLYDAPVLAGLDRRWSRLICGWLLVVLFLTLILFLLKAGAEVSRGSMIVFGFVGCTALVVGRAVFERPLRKAIDRGVLA
TRRAIVVGSPDELSRLRPVDLLKNYGLSEVHRIFLSAKPDSDAASEDGAVSQAVIQRARELRVDEIVLALRWSDEARIYR
ICELFRASPLPIHLLPDQTAEQFLSLPILATGPLPTVEMQRAPLSRLERTCKRAFDILAASALLIVFFPVMVAAALSVKL
DSAGPIIFRQRRNGFDGRTFRIFKFRTMLVQEDGPKVEQAQPNDPRVTRVGRILRRRSIDELPQLLNVLRGEMSLVGPRP
HAIAHDSEFSKLIASYAYRQHVKPGITGLAQVRGLRGPTPAVEDMQKRVEHDLAYISSWSFTLDLHILLRTVRVFFLSNA
D

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=292, Percent_Identity=41.7808219178082, Blast_Score=242, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 53725; Mature: 53593

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELSESRAERRTAIYFGAASANVGLLELLAIVYEMLVVFGASLIAGYAYHITYYGVFQK
CCCCHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LESFVGIGIFAAVLHMFIAKSQGLYDAPVLAGLDRRWSRLICGWLLVVLFLTLILFLLKA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAEVSRGSMIVFGFVGCTALVVGRAVFERPLRKAIDRGVLATRRAIVVGSPDELSRLRPV
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEECCCHHHHHCCHH
DLLKNYGLSEVHRIFLSAKPDSDAASEDGAVSQAVIQRARELRVDEIVLALRWSDEARIY
HHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEEEEEECCCHHHH
RICELFRASPLPIHLLPDQTAEQFLSLPILATGPLPTVEMQRAPLSRLERTCKRAFDILA
HHHHHHCCCCCCEEECCCHHHHHHHCCCCEECCCCCCHHHHHCHHHHHHHHHHHHHHHHH
ASALLIVFFPVMVAAALSVKLDSAGPIIFRQRRNGFDGRTFRIFKFRTMLVQEDGPKVEQ
HHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCEEEEEEHHHHHHCCCCCCCCC
AQPNDPRVTRVGRILRRRSIDELPQLLNVLRGEMSLVGPRPHAIAHDSEFSKLIASYAYR
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHH
QHVKPGITGLAQVRGLRGPTPAVEDMQKRVEHDLAYISSWSFTLDLHILLRTVRVFFLSN
HHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
AD
CC
>Mature Secondary Structure 
TELSESRAERRTAIYFGAASANVGLLELLAIVYEMLVVFGASLIAGYAYHITYYGVFQK
CCCHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LESFVGIGIFAAVLHMFIAKSQGLYDAPVLAGLDRRWSRLICGWLLVVLFLTLILFLLKA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAEVSRGSMIVFGFVGCTALVVGRAVFERPLRKAIDRGVLATRRAIVVGSPDELSRLRPV
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEECCCHHHHHCCHH
DLLKNYGLSEVHRIFLSAKPDSDAASEDGAVSQAVIQRARELRVDEIVLALRWSDEARIY
HHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEEEEEECCCHHHH
RICELFRASPLPIHLLPDQTAEQFLSLPILATGPLPTVEMQRAPLSRLERTCKRAFDILA
HHHHHHCCCCCCEEECCCHHHHHHHCCCCEECCCCCCHHHHHCHHHHHHHHHHHHHHHHH
ASALLIVFFPVMVAAALSVKLDSAGPIIFRQRRNGFDGRTFRIFKFRTMLVQEDGPKVEQ
HHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCEEEEEEHHHHHHCCCCCCCCC
AQPNDPRVTRVGRILRRRSIDELPQLLNVLRGEMSLVGPRPHAIAHDSEFSKLIASYAYR
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHH
QHVKPGITGLAQVRGLRGPTPAVEDMQKRVEHDLAYISSWSFTLDLHILLRTVRVFFLSN
HHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
AD
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]