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The map label for this gene is lcfB [H]

Identifier: 146343630

GI number: 146343630

Start: 7135674

End: 7137224

Strand: Direct

Name: lcfB [H]

Synonym: BRADO6869

Alternate gene names: 146343630

Gene position: 7135674-7137224 (Clockwise)

Preceding gene: 146343628

Following gene: 146343631

Centisome position: 95.7

GC content: 65.76

Gene sequence:

>1551_bases
ATGTACACCGGCAAGCACGCAAGGCTGCGCCCGCTGCAGCCCGCCTTCATCATGGCCTCCACCGGCGAAGCGGTGAACTA
TCGCGAGCTGGAGGCGCGCTCGAACCGCCTAGCGCATCTCTTTCGCAAGCGTGGCCTGAAGCGGCTGGACCATTATTCGA
TCTTCATGGAGAACAACAGCCGCTATCTCGAAGCCTGCGGCGCCGGCGAGCGCTCCGGCCTGTATTTCACCTGCGTGAAC
TCGTACCTCACGCCGGGCGAGCTCGCCTACATCCTCAATAACAGCCAGTCCCGGCTCCTGATCACCTCGGTCGCGAAGCT
CGACGTCGCGCGCGAGGCGCTCAAGGAGGCGCCCGGCATCGAGCTGTGCATGGTCGCCGACGGTCCGGGAGAGGGCGATC
GCATCGTCGGCCTGCAGGAGGCGATCGCCGGCCTCCCGGCGACGCCGATCGCCGACGAATGCATCGGCACGGCGATGCTG
TACTCCTCCGGCACAACCGGCCGGCCCAAGGGCATCCTGCGGCCGCTGCCGGAGCAGCCGCCGGTGCAGCAGTTGCCGAT
CTTCGACTTCCTCGAGAAGCTGTGGCGCTACCGCGAGGGCATGATCTATCTGTCGCCGGCGCCGCTGTATCACTCCGCGC
CACAAGCCGCCGTCAATCTCACGATCCGCGCTGGCGGCACCGCCATCATCATGGAGAATTTCGATCCGGAGCGCTACCTG
CAGCTCGTCGAGCAATGGGGCATCACCCATACCCAGCTGGTGCCGACGATGTTCTCGCGCATGCTGAAGCTGCCGGAGGA
GATCCGCACCCGCTACGATCTGTCCTCGCTGGAGATCGCGATCCATGCCGCGGCGCCCTGCCCCGCGCAGGTCAAGGAGG
ACATGATCCGCTGGTGGGGGCCGATCATCCACGAATATTACGGCGCCACCGAAGGCCTCGGCTTCACCGCCTGCGACAGC
GAGCAATGGCTCGCGCATCGCGGCACCGTCGGCAAGGTGCTGTTCGGCGACCTGCACATCCTCGACGAACACATGCAGCC
TTGCCCGGCCGGCACCGCCGGCACGGTGTGGTTCAAGACCGGTTCGCCGTTCGAGTATTTCAACGACCCCGAGCGCACCC
GGGAGGCGCGCTCGGCCGACGGCACGATGAGCACGGTCGGCGATGTCGGCTATGTCGATGCCGACGGCTATCTGTATCTC
ACCGACCGCGCCACCTTCATGATCATCTCGGGCGGCGTGAACATCTATCCGCAGGAATGCGAGAACCTGCTGATCACGCA
TCCGAAGGTCGCCGACGCCGCGGTGTTCGGCGTGCCCAATACCGATCTCGGCGAGGAGGTGAAGGCGGTGATCCAGCCGA
TGTCCGGCATCGTGCCCAGTCCTGCGCTCGAGGAGGAGCTGATCGCGTTCTGCCGGCAGTCGCTGTCGCGCCAGAAGGTG
CCGCGCTCGATCGACTTCGAGACGGAGCTGCCGCGGCTGCCGACCGGCAAGCTCTACAAGCGTCTGCTGCGCGACCGCTA
TTGGGGCAACAAGACCTCGCGGATCGTGTAG

Upstream 100 bases:

>100_bases
GTCGTGACGCGAGCCATCGCCCGCGCCCCGTCATCGTGCTCCCTCCTGCGCTCGCGCCGCCCTGCGGAATATGCGGCAAT
CGCTGCCCGAGGGAGGACAC

Downstream 100 bases:

>100_bases
GACGCCTGACCGGAGGTTGATCCTGGTCAAGCGGGCGCGGCGCGACGTCGCTATGCTTCGCCGATTGCGACCGCAGCGAT
TTCGCGAGACGACGACATGC

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MYTGKHARLRPLQPAFIMASTGEAVNYRELEARSNRLAHLFRKRGLKRLDHYSIFMENNSRYLEACGAGERSGLYFTCVN
SYLTPGELAYILNNSQSRLLITSVAKLDVAREALKEAPGIELCMVADGPGEGDRIVGLQEAIAGLPATPIADECIGTAML
YSSGTTGRPKGILRPLPEQPPVQQLPIFDFLEKLWRYREGMIYLSPAPLYHSAPQAAVNLTIRAGGTAIIMENFDPERYL
QLVEQWGITHTQLVPTMFSRMLKLPEEIRTRYDLSSLEIAIHAAAPCPAQVKEDMIRWWGPIIHEYYGATEGLGFTACDS
EQWLAHRGTVGKVLFGDLHILDEHMQPCPAGTAGTVWFKTGSPFEYFNDPERTREARSADGTMSTVGDVGYVDADGYLYL
TDRATFMIISGGVNIYPQECENLLITHPKVADAAVFGVPNTDLGEEVKAVIQPMSGIVPSPALEEELIAFCRQSLSRQKV
PRSIDFETELPRLPTGKLYKRLLRDRYWGNKTSRIV

Sequences:

>Translated_516_residues
MYTGKHARLRPLQPAFIMASTGEAVNYRELEARSNRLAHLFRKRGLKRLDHYSIFMENNSRYLEACGAGERSGLYFTCVN
SYLTPGELAYILNNSQSRLLITSVAKLDVAREALKEAPGIELCMVADGPGEGDRIVGLQEAIAGLPATPIADECIGTAML
YSSGTTGRPKGILRPLPEQPPVQQLPIFDFLEKLWRYREGMIYLSPAPLYHSAPQAAVNLTIRAGGTAIIMENFDPERYL
QLVEQWGITHTQLVPTMFSRMLKLPEEIRTRYDLSSLEIAIHAAAPCPAQVKEDMIRWWGPIIHEYYGATEGLGFTACDS
EQWLAHRGTVGKVLFGDLHILDEHMQPCPAGTAGTVWFKTGSPFEYFNDPERTREARSADGTMSTVGDVGYVDADGYLYL
TDRATFMIISGGVNIYPQECENLLITHPKVADAAVFGVPNTDLGEEVKAVIQPMSGIVPSPALEEELIAFCRQSLSRQKV
PRSIDFETELPRLPTGKLYKRLLRDRYWGNKTSRIV
>Mature_516_residues
MYTGKHARLRPLQPAFIMASTGEAVNYRELEARSNRLAHLFRKRGLKRLDHYSIFMENNSRYLEACGAGERSGLYFTCVN
SYLTPGELAYILNNSQSRLLITSVAKLDVAREALKEAPGIELCMVADGPGEGDRIVGLQEAIAGLPATPIADECIGTAML
YSSGTTGRPKGILRPLPEQPPVQQLPIFDFLEKLWRYREGMIYLSPAPLYHSAPQAAVNLTIRAGGTAIIMENFDPERYL
QLVEQWGITHTQLVPTMFSRMLKLPEEIRTRYDLSSLEIAIHAAAPCPAQVKEDMIRWWGPIIHEYYGATEGLGFTACDS
EQWLAHRGTVGKVLFGDLHILDEHMQPCPAGTAGTVWFKTGSPFEYFNDPERTREARSADGTMSTVGDVGYVDADGYLYL
TDRATFMIISGGVNIYPQECENLLITHPKVADAAVFGVPNTDLGEEVKAVIQPMSGIVPSPALEEELIAFCRQSLSRQKV
PRSIDFETELPRLPTGKLYKRLLRDRYWGNKTSRIV

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI122937307, Length=527, Percent_Identity=27.5142314990512, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI156151445, Length=546, Percent_Identity=25.4578754578755, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI42544132, Length=530, Percent_Identity=26.4150943396226, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI38505220, Length=532, Percent_Identity=26.3157894736842, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI187761345, Length=521, Percent_Identity=27.2552783109405, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI187761343, Length=521, Percent_Identity=27.2552783109405, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI115511026, Length=517, Percent_Identity=24.7582205029014, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI157311624, Length=503, Percent_Identity=23.2604373757455, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI157311622, Length=503, Percent_Identity=23.2604373757455, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI58082049, Length=509, Percent_Identity=23.3791748526523, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI13375727, Length=536, Percent_Identity=22.5746268656716, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI221142682, Length=519, Percent_Identity=27.7456647398844, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1788107, Length=535, Percent_Identity=27.8504672897196, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI145693145, Length=528, Percent_Identity=25, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790505, Length=520, Percent_Identity=24.0384615384615, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1786810, Length=495, Percent_Identity=23.6363636363636, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786529, Length=538, Percent_Identity=21.3754646840149, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17531443, Length=534, Percent_Identity=26.5917602996255, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17558820, Length=509, Percent_Identity=28.0943025540275, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17559526, Length=511, Percent_Identity=25.440313111546, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32563687, Length=450, Percent_Identity=26.6666666666667, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17538037, Length=533, Percent_Identity=26.6416510318949, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=544, Percent_Identity=25.3676470588235, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=354, Percent_Identity=28.2485875706215, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=354, Percent_Identity=28.2485875706215, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=354, Percent_Identity=28.2485875706215, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=351, Percent_Identity=27.9202279202279, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=357, Percent_Identity=26.0504201680672, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115534440, Length=545, Percent_Identity=22.9357798165138, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17551278, Length=563, Percent_Identity=22.9129662522202, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=363, Percent_Identity=27.2727272727273, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21355181, Length=501, Percent_Identity=25.9481037924152, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24581924, Length=541, Percent_Identity=23.6598890942699, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19922652, Length=437, Percent_Identity=23.5697940503432, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648253, Length=460, Percent_Identity=24.3478260869565, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI18859661, Length=499, Percent_Identity=23.4468937875752, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648260, Length=521, Percent_Identity=23.8003838771593, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648255, Length=460, Percent_Identity=24.3478260869565, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356441, Length=513, Percent_Identity=23.7816764132554, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356947, Length=521, Percent_Identity=23.9923224568138, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281365686, Length=323, Percent_Identity=24.7678018575851, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21358303, Length=451, Percent_Identity=21.0643015521064, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24653035, Length=503, Percent_Identity=21.4711729622266, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI161076582, Length=516, Percent_Identity=21.3178294573643, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24656500, Length=431, Percent_Identity=22.0417633410673, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648257, Length=498, Percent_Identity=22.8915662650602, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21355225, Length=367, Percent_Identity=23.9782016348774, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45552801, Length=484, Percent_Identity=21.0743801652893, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550496, Length=484, Percent_Identity=21.0743801652893, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 57508; Mature: 57508

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTGKHARLRPLQPAFIMASTGEAVNYRELEARSNRLAHLFRKRGLKRLDHYSIFMENNS
CCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEECCC
RYLEACGAGERSGLYFTCVNSYLTPGELAYILNNSQSRLLITSVAKLDVAREALKEAPGI
EEEEECCCCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCC
ELCMVADGPGEGDRIVGLQEAIAGLPATPIADECIGTAMLYSSGTTGRPKGILRPLPEQP
EEEEEECCCCCCCEEEEHHHHHCCCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCCC
PVQQLPIFDFLEKLWRYREGMIYLSPAPLYHSAPQAAVNLTIRAGGTAIIMENFDPERYL
CCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEECCCEEEEEECCCHHHHH
QLVEQWGITHTQLVPTMFSRMLKLPEEIRTRYDLSSLEIAIHAAAPCPAQVKEDMIRWWG
HHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHH
PIIHEYYGATEGLGFTACDSEQWLAHRGTVGKVLFGDLHILDEHMQPCPAGTAGTVWFKT
HHHHHHHCCCCCCCEEECCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEC
GSPFEYFNDPERTREARSADGTMSTVGDVGYVDADGYLYLTDRATFMIISGGVNIYPQEC
CCCHHHCCCHHHHHHHHCCCCCHHHHCCCCEEECCCEEEEECCEEEEEEECCCEECCHHH
ENLLITHPKVADAAVFGVPNTDLGEEVKAVIQPMSGIVPSPALEEELIAFCRQSLSRQKV
CCEEECCCCHHCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
PRSIDFETELPRLPTGKLYKRLLRDRYWGNKTSRIV
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MYTGKHARLRPLQPAFIMASTGEAVNYRELEARSNRLAHLFRKRGLKRLDHYSIFMENNS
CCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEECCC
RYLEACGAGERSGLYFTCVNSYLTPGELAYILNNSQSRLLITSVAKLDVAREALKEAPGI
EEEEECCCCCCCCEEEEEECCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCC
ELCMVADGPGEGDRIVGLQEAIAGLPATPIADECIGTAMLYSSGTTGRPKGILRPLPEQP
EEEEEECCCCCCCEEEEHHHHHCCCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCCC
PVQQLPIFDFLEKLWRYREGMIYLSPAPLYHSAPQAAVNLTIRAGGTAIIMENFDPERYL
CCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEECCCEEEEEECCCHHHHH
QLVEQWGITHTQLVPTMFSRMLKLPEEIRTRYDLSSLEIAIHAAAPCPAQVKEDMIRWWG
HHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHH
PIIHEYYGATEGLGFTACDSEQWLAHRGTVGKVLFGDLHILDEHMQPCPAGTAGTVWFKT
HHHHHHHCCCCCCCEEECCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEC
GSPFEYFNDPERTREARSADGTMSTVGDVGYVDADGYLYLTDRATFMIISGGVNIYPQEC
CCCHHHCCCHHHHHHHHCCCCCHHHHCCCCEEECCCEEEEECCEEEEEEECCCEECCHHH
ENLLITHPKVADAAVFGVPNTDLGEEVKAVIQPMSGIVPSPALEEELIAFCRQSLSRQKV
CCEEECCCCHHCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
PRSIDFETELPRLPTGKLYKRLLRDRYWGNKTSRIV
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]