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The map label for this gene is ptrB [C]

Identifier: 146343606

GI number: 146343606

Start: 7106301

End: 7108367

Strand: Direct

Name: ptrB [C]

Synonym: BRADO6844

Alternate gene names: 146343606

Gene position: 7106301-7108367 (Clockwise)

Preceding gene: 146343604

Following gene: 146343608

Centisome position: 95.3

GC content: 68.7

Gene sequence:

>2067_bases
ATGTCAATCGACGATCGTCCGACCCTCGCCGCGCCCGACGACGATCCGTATCTGTGGCTCGAGGATATCGAAGGCGAGCG
CCCGCTCGCCTTCGTCGCGGCGCAGAATGAGCGCACGCTTGCCGCGTTCGGCGGCGCCGCCTTCGCGCGGGATCGCGACG
CGCTGGCCGCGATCTTCGATCGCCCCGACAACATCCCCTTCGTCAGCCGCCGCGGTGGGCTGCTGTACAATCAATGGAAA
GACGCGACTCATCCGCGCGGCTTGTGGCGGCGCACGACAATGGACGAGTTCCGCAAAGCGCAACCCAAATGGGAGCTGCT
GCTCGACATCGATCGCCTCGCCGCCGAGGAAGGCGAGGACTGGGCGCTGAACTGGGTCTCGACCCTGCCGCCGGCGCATG
AGCGCGCGATCCTGGCGCTGTCGCGCGGCGGCAGCGACGCGGTGGTGCTGCGCGAGTTCGATCTCACCACGAAGCGCTTC
GTCGAGGGCGGCTTCACGTTGAGCGAGGCCAAGGGCGGCGTCGACTGGGTTGACGCCGATACGCTGCTGCTGTCGAGCGC
TTACGGCGAGGGCATGGCGACGCCCTCAGGCTATGCGCGCACGGTGCGGCTGTGGCGACGCGGCGCGCCGGTCGACGCGG
CGCCGGTCATCTTTGCCGCTGCGAATGAGCAGTCGGTGTGGGTGCGCGCTGGTGTCGATCGCAGCGTCACGCCGTTCAGG
GTGTGGTTTTCGGAAGGGCTCGACTTCTTCAACCATCATCTGTGGCTCGGCGATGCCGGCGGCGCGCGGACGCGGCTCGA
CCTGCCGACCGACAGCTGGGCCATACCGGACGCGGACTGGCTGGCGATCAAGCTGCGCCAGCCCTGGACCGTGGGCGGGA
TCACGCATGCGGCGGATGTCGTGCTGGGGATCTCCTTGTCGGCATGTCTCGCCGGCTCGCCCGCGTTCGCTAAACTCTTC
GAGCCGGGCCCGCGCCGCGCGCTGCAGGGGCTGTACTGGGCCGGCGGCCGGCTGGTGCTGTCGATCCTCGATGAGCTCAA
GCCGCAATACGAGGTGTGGACGCCGGCGGCGCAGGGCTGGAGCCGTCGGCAGCTGCCGGGATTGCCCGAGCTGGCCACCG
TCAACGTCTGGCCGCTCGACAGCGACGAGCTGACCGGCAATGGCGATCTGCTCGCCAGCGTCCAGGACCCGCTGACGCCG
CCGTCGCTGCAGTTAATTGAGAGTTTCGGCGCGCCCGCCGTGCTGAAGCAGGCGCCGCGCACCTTCACCGCCGACGGATA
CGTGGTCACGCGATACGAGGCGGCCTCGGTGGATGGCGTCGGCATTCCCTACACGCTGACCGGCCCCGCGAAGCTCACCG
GCGATGCGCCGGTGTATATGAGTGCCTATGGCGGCTTCGGCCACAGCATCCGTCCGGCCTACAATGCCGCGCTCGGCAAG
CTGTGGCTGGAGCGCGGCGGCAGCCTGGTCGAAGCGAACATCCGCGGCGGCGGCGAGTTCGGCACCGCCTGGCACGACGC
CGGACGCTACGCCGGCAAGCGGCTCGCGCATGATGATTTCGCCGCCGTGGCCGCCGATCTCGTCCGCCGCGGCATCACGG
TGCCGAAGCGCATCGCCGCGCAAGGCGGCTCCAATGGCGGCATCCTGATCACCAACATGCTGGTGCGCTATCCCGAGCGC
TTCGGCGCGCTGTTCTGCACCATCCCGCTGATCGACATGCGCCGTTATACGAAGCTTCTGGCGGGCGCGAGCTGGATCGC
CGAGTATGGCGATCCGGACAAGGCCGAGGACTGGGGCTGGCTGCAGACCTACTCCGCCTATCACACCGCAAAGCCCGGCC
AGCCTTATCCGCCCATCCTGATCGCCACGACGCGGCGCGACGACCGCGTGCATCCGGGACATGCGCGCAAGATGACGGCG
AAGCTGCAGGCGATGGGCTATGACAGCTATTTCTACGAGCCCGCCGCCGGCGGCCACGGCTACGGCACCGACAACCGCGA
GCGCGCCAGCTTCATCACCCTCGGCACGCGGTTTCTGGCGCGGCATGTGGGGCTGGAGATGGGATAG

Upstream 100 bases:

>100_bases
GAGGCAAGCCATGCACGACGGTTGATTCGACAGCTCCGGCCGGCCCGGCTAATCCAGCCAGCGGTATTGTCGCGTCAACG
GCCGCTGCGGAGCTTGAGCC

Downstream 100 bases:

>100_bases
CGACTGCGCTTCTTTGTAAGGCGCGCTGCCGGCCTCACTTACCACCGTCATCCCGGCGAACACCGGGATCCATACTCCGC
AGCAGGAGTGTGTTGCGACG

Product: putative prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MSIDDRPTLAAPDDDPYLWLEDIEGERPLAFVAAQNERTLAAFGGAAFARDRDALAAIFDRPDNIPFVSRRGGLLYNQWK
DATHPRGLWRRTTMDEFRKAQPKWELLLDIDRLAAEEGEDWALNWVSTLPPAHERAILALSRGGSDAVVLREFDLTTKRF
VEGGFTLSEAKGGVDWVDADTLLLSSAYGEGMATPSGYARTVRLWRRGAPVDAAPVIFAAANEQSVWVRAGVDRSVTPFR
VWFSEGLDFFNHHLWLGDAGGARTRLDLPTDSWAIPDADWLAIKLRQPWTVGGITHAADVVLGISLSACLAGSPAFAKLF
EPGPRRALQGLYWAGGRLVLSILDELKPQYEVWTPAAQGWSRRQLPGLPELATVNVWPLDSDELTGNGDLLASVQDPLTP
PSLQLIESFGAPAVLKQAPRTFTADGYVVTRYEAASVDGVGIPYTLTGPAKLTGDAPVYMSAYGGFGHSIRPAYNAALGK
LWLERGGSLVEANIRGGGEFGTAWHDAGRYAGKRLAHDDFAAVAADLVRRGITVPKRIAAQGGSNGGILITNMLVRYPER
FGALFCTIPLIDMRRYTKLLAGASWIAEYGDPDKAEDWGWLQTYSAYHTAKPGQPYPPILIATTRRDDRVHPGHARKMTA
KLQAMGYDSYFYEPAAGGHGYGTDNRERASFITLGTRFLARHVGLEMG

Sequences:

>Translated_688_residues
MSIDDRPTLAAPDDDPYLWLEDIEGERPLAFVAAQNERTLAAFGGAAFARDRDALAAIFDRPDNIPFVSRRGGLLYNQWK
DATHPRGLWRRTTMDEFRKAQPKWELLLDIDRLAAEEGEDWALNWVSTLPPAHERAILALSRGGSDAVVLREFDLTTKRF
VEGGFTLSEAKGGVDWVDADTLLLSSAYGEGMATPSGYARTVRLWRRGAPVDAAPVIFAAANEQSVWVRAGVDRSVTPFR
VWFSEGLDFFNHHLWLGDAGGARTRLDLPTDSWAIPDADWLAIKLRQPWTVGGITHAADVVLGISLSACLAGSPAFAKLF
EPGPRRALQGLYWAGGRLVLSILDELKPQYEVWTPAAQGWSRRQLPGLPELATVNVWPLDSDELTGNGDLLASVQDPLTP
PSLQLIESFGAPAVLKQAPRTFTADGYVVTRYEAASVDGVGIPYTLTGPAKLTGDAPVYMSAYGGFGHSIRPAYNAALGK
LWLERGGSLVEANIRGGGEFGTAWHDAGRYAGKRLAHDDFAAVAADLVRRGITVPKRIAAQGGSNGGILITNMLVRYPER
FGALFCTIPLIDMRRYTKLLAGASWIAEYGDPDKAEDWGWLQTYSAYHTAKPGQPYPPILIATTRRDDRVHPGHARKMTA
KLQAMGYDSYFYEPAAGGHGYGTDNRERASFITLGTRFLARHVGLEMG
>Mature_687_residues
SIDDRPTLAAPDDDPYLWLEDIEGERPLAFVAAQNERTLAAFGGAAFARDRDALAAIFDRPDNIPFVSRRGGLLYNQWKD
ATHPRGLWRRTTMDEFRKAQPKWELLLDIDRLAAEEGEDWALNWVSTLPPAHERAILALSRGGSDAVVLREFDLTTKRFV
EGGFTLSEAKGGVDWVDADTLLLSSAYGEGMATPSGYARTVRLWRRGAPVDAAPVIFAAANEQSVWVRAGVDRSVTPFRV
WFSEGLDFFNHHLWLGDAGGARTRLDLPTDSWAIPDADWLAIKLRQPWTVGGITHAADVVLGISLSACLAGSPAFAKLFE
PGPRRALQGLYWAGGRLVLSILDELKPQYEVWTPAAQGWSRRQLPGLPELATVNVWPLDSDELTGNGDLLASVQDPLTPP
SLQLIESFGAPAVLKQAPRTFTADGYVVTRYEAASVDGVGIPYTLTGPAKLTGDAPVYMSAYGGFGHSIRPAYNAALGKL
WLERGGSLVEANIRGGGEFGTAWHDAGRYAGKRLAHDDFAAVAADLVRRGITVPKRIAAQGGSNGGILITNMLVRYPERF
GALFCTIPLIDMRRYTKLLAGASWIAEYGDPDKAEDWGWLQTYSAYHTAKPGQPYPPILIATTRRDDRVHPGHARKMTAK
LQAMGYDSYFYEPAAGGHGYGTDNRERASFITLGTRFLARHVGLEMG

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=304, Percent_Identity=33.5526315789474, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI284172438, Length=276, Percent_Identity=25.7246376811594, Blast_Score=71, Evalue=4e-12,
Organism=Homo sapiens, GI284172431, Length=276, Percent_Identity=25.7246376811594, Blast_Score=71, Evalue=4e-12,
Organism=Homo sapiens, GI284172420, Length=276, Percent_Identity=25.7246376811594, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI284172413, Length=276, Percent_Identity=25.7246376811594, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI70778815, Length=276, Percent_Identity=25.7246376811594, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI108860686, Length=277, Percent_Identity=25.6317689530686, Blast_Score=70, Evalue=8e-12,
Organism=Escherichia coli, GI1788150, Length=262, Percent_Identity=28.2442748091603, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI221510989, Length=293, Percent_Identity=32.0819112627986, Blast_Score=154, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24583414, Length=293, Percent_Identity=31.0580204778157, Blast_Score=148, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 75248; Mature: 75116

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDDRPTLAAPDDDPYLWLEDIEGERPLAFVAAQNERTLAAFGGAAFARDRDALAAIFD
CCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEECCHHHHCCHHHHHHHHC
RPDNIPFVSRRGGLLYNQWKDATHPRGLWRRTTMDEFRKAQPKWELLLDIDRLAAEEGED
CCCCCCEEECCCCEEEECCCCCCCCCCHHHHCCHHHHHHCCCCEEEEEEHHHHHHCCCCC
WALNWVSTLPPAHERAILALSRGGSDAVVLREFDLTTKRFVEGGFTLSEAKGGVDWVDAD
EEEEEHHHCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHCCCCEEECCCCCCCEECHH
TLLLSSAYGEGMATPSGYARTVRLWRRGAPVDAAPVIFAAANEQSVWVRAGVDRSVTPFR
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCCCCHHH
VWFSEGLDFFNHHLWLGDAGGARTRLDLPTDSWAIPDADWLAIKLRQPWTVGGITHAADV
HHHHHCHHHHHCEEEEECCCCCCEEEECCCCCCCCCCCCEEEEEEECCEEECCHHHHHHH
VLGISLSACLAGSPAFAKLFEPGPRRALQGLYWAGGRLVLSILDELKPQYEVWTPAAQGW
HHHCCHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCHHCCC
SRRQLPGLPELATVNVWPLDSDELTGNGDLLASVQDPLTPPSLQLIESFGAPAVLKQAPR
CCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCHHHHHCCC
TFTADGYVVTRYEAASVDGVGIPYTLTGPAKLTGDAPVYMSAYGGFGHSIRPAYNAALGK
EEECCCEEEEEEECCCCCCCCCCEEECCCCEECCCCCEEEEECCCCCCCCCHHHHHHHHH
LWLERGGSLVEANIRGGGEFGTAWHDAGRYAGKRLAHDDFAAVAADLVRRGITVPKRIAA
HHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHH
QGGSNGGILITNMLVRYPERFGALFCTIPLIDMRRYTKLLAGASWIAEYGDPDKAEDWGW
CCCCCCCEEHHHHHHHCHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
LQTYSAYHTAKPGQPYPPILIATTRRDDRVHPGHARKMTAKLQAMGYDSYFYEPAAGGHG
HHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCC
YGTDNRERASFITLGTRFLARHVGLEMG
CCCCCCCHHHEEEHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIDDRPTLAAPDDDPYLWLEDIEGERPLAFVAAQNERTLAAFGGAAFARDRDALAAIFD
CCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEECCHHHHCCHHHHHHHHC
RPDNIPFVSRRGGLLYNQWKDATHPRGLWRRTTMDEFRKAQPKWELLLDIDRLAAEEGED
CCCCCCEEECCCCEEEECCCCCCCCCCHHHHCCHHHHHHCCCCEEEEEEHHHHHHCCCCC
WALNWVSTLPPAHERAILALSRGGSDAVVLREFDLTTKRFVEGGFTLSEAKGGVDWVDAD
EEEEEHHHCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHCCCCEEECCCCCCCEECHH
TLLLSSAYGEGMATPSGYARTVRLWRRGAPVDAAPVIFAAANEQSVWVRAGVDRSVTPFR
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCCCCHHH
VWFSEGLDFFNHHLWLGDAGGARTRLDLPTDSWAIPDADWLAIKLRQPWTVGGITHAADV
HHHHHCHHHHHCEEEEECCCCCCEEEECCCCCCCCCCCCEEEEEEECCEEECCHHHHHHH
VLGISLSACLAGSPAFAKLFEPGPRRALQGLYWAGGRLVLSILDELKPQYEVWTPAAQGW
HHHCCHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCHHCCC
SRRQLPGLPELATVNVWPLDSDELTGNGDLLASVQDPLTPPSLQLIESFGAPAVLKQAPR
CCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCHHHHHCCC
TFTADGYVVTRYEAASVDGVGIPYTLTGPAKLTGDAPVYMSAYGGFGHSIRPAYNAALGK
EEECCCEEEEEEECCCCCCCCCCEEECCCCEECCCCCEEEEECCCCCCCCCHHHHHHHHH
LWLERGGSLVEANIRGGGEFGTAWHDAGRYAGKRLAHDDFAAVAADLVRRGITVPKRIAA
HHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHH
QGGSNGGILITNMLVRYPERFGALFCTIPLIDMRRYTKLLAGASWIAEYGDPDKAEDWGW
CCCCCCCEEHHHHHHHCHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
LQTYSAYHTAKPGQPYPPILIATTRRDDRVHPGHARKMTAKLQAMGYDSYFYEPAAGGHG
HHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCC
YGTDNRERASFITLGTRFLARHVGLEMG
CCCCCCCHHHEEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]