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The map label for this gene is gatA [H]

Identifier: 146343580

GI number: 146343580

Start: 7075590

End: 7077017

Strand: Reverse

Name: gatA [H]

Synonym: BRADO6818

Alternate gene names: 146343580

Gene position: 7077017-7075590 (Counterclockwise)

Preceding gene: 146343581

Following gene: 146343579

Centisome position: 94.91

GC content: 67.3

Gene sequence:

>1428_bases
GTGTCTTCACCGCTTGATTCTTCCGAGATGACCGCAGGCGCCATTGCCGCCGCGGTCGCCGCCCGCAAGCTCACCGCTGT
CGAGGCCACCGACGCCGCGCTGGCGCGGATCGCGGCCCGCGATGGTGTGCTCAATTCCTTTACCGACATCACCGCCGAGC
GGGCGCGGGCGAAGGCACGCGCCGTCGATGCCGATATCGCGGCCGGAAAACCTGTCGGGCCGCTCGCCGGGGTGCCGTTC
GCGGTAAAGAACCTGTTCGACGTCGCTGATCTGCCGACCCGCGCCGGCTCCAAGATCAATCGTGATCGCGCACCCGCGCC
GCGCGACTCGACGCTGATCGAGCGAATGGAAGCCGCGGGCGCCGTTCTCGTTGGCGCGCTCAACATGGGCGAATACGCCT
ATGACTTCACCGGCGAGAACATCCATGATGGCCCGTCGCGCAATCCGCATGATCCGACCCGGATGAGCGGCGGCTCGTCG
GGCGGCTCGGGCAGCGCCGTCGGCGGCGGCCTGGTGCCGATCGCGCTCGGCTCCGACACCAATGGCTCGATCCGCGTGCC
CTCGTCGTTCTGCGGCATCTTCGGTCTGAAGCCGACCTATGGTCGGCTGTCGCGCGCGCGCTCGTTTCCGTTCGTGATGA
GCCTCGATCATCTCGGTCCGTTCGCCCGCTCGGTCGAGGATCTCGCGCTTGCCTATGACGTGATGCAGGGGCCGGATTCC
GACGATGCCGCTTGCACCTCGCGTGCTCCGGAGCCGGTGTCTGGCCAGCTCTCGCAGGGGCTCGACGGTCTGCGCATCGC
GATCGCCGGCGGTCACTTTCAGAAGAACCTGTTTCCCGAAGCGGCCGAAGCCGTCAGTCGCGTTGTCGCCGCTCTGAAGG
TGACGCAGGTCATCGAGGTGCCCGAAGCGGCGCGCGCCCGCGCTGCGGCCTACGTCATCAGCACGACCGAAGGTGCATCG
CTGCATCTCGACCGGCTGCGCACGCGTCCCGATGATTTCGATCCCGCGGTGCGCGATCGTTTGATCGCCGGCGCGATGAT
CCCGGCGCCGATGGTCGACCGCGCGCAGAAATTCCGCCGCTGGTATCGGGCGAAGGTGCTGGAGCTGTTCAGATCGGTCG
ACGTGCTGATCGCGCCCGCGACGCCATGCGTCGCGCCGAAACTGGGGCAGGCAACCTTCGTGCTGGATGGCGTCGAGCTA
CCGGTGCGCGCCAATATCGGCATCCATACCCAGCCGATCTCGTTCATCGGACTTCCCGTCGTCGCCGTGCCGATTCCGCT
CGCGCCGATGCCCATCGGCGTGCAGATCATCGCCGCGCCCTGGTGTGAACATGTCGCATTGCGTGTGGCCCACGCCTTGC
AACAGCTTGGCGTGGCCGCCGCGCCGGCCCCGAGAGGAGTTCTGGAGAATGGAAGTCGATCTGCCTGA

Upstream 100 bases:

>100_bases
GACGCATGGCGGCCGGCGATCCGCGCCAATCTCGAGGTCTCGCTGAAAATGGCGAAGCTGGTCGATGAATTCTCCCTGCC
TGACGAAGCCGAGCCTGCCA

Downstream 100 bases:

>100_bases
CGTCGTCGCGGAGGTCACCGCCCAGTTCCAGCGGTATGAACAGGCCCTGGTCAGCAACGACGTCGCCGTGCTCGACGAGC
TGTTTCGCAACGACGCGCGA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSSPLDSSEMTAGAIAAAVAARKLTAVEATDAALARIAARDGVLNSFTDITAERARAKARAVDADIAAGKPVGPLAGVPF
AVKNLFDVADLPTRAGSKINRDRAPAPRDSTLIERMEAAGAVLVGALNMGEYAYDFTGENIHDGPSRNPHDPTRMSGGSS
GGSGSAVGGGLVPIALGSDTNGSIRVPSSFCGIFGLKPTYGRLSRARSFPFVMSLDHLGPFARSVEDLALAYDVMQGPDS
DDAACTSRAPEPVSGQLSQGLDGLRIAIAGGHFQKNLFPEAAEAVSRVVAALKVTQVIEVPEAARARAAAYVISTTEGAS
LHLDRLRTRPDDFDPAVRDRLIAGAMIPAPMVDRAQKFRRWYRAKVLELFRSVDVLIAPATPCVAPKLGQATFVLDGVEL
PVRANIGIHTQPISFIGLPVVAVPIPLAPMPIGVQIIAAPWCEHVALRVAHALQQLGVAAAPAPRGVLENGSRSA

Sequences:

>Translated_475_residues
MSSPLDSSEMTAGAIAAAVAARKLTAVEATDAALARIAARDGVLNSFTDITAERARAKARAVDADIAAGKPVGPLAGVPF
AVKNLFDVADLPTRAGSKINRDRAPAPRDSTLIERMEAAGAVLVGALNMGEYAYDFTGENIHDGPSRNPHDPTRMSGGSS
GGSGSAVGGGLVPIALGSDTNGSIRVPSSFCGIFGLKPTYGRLSRARSFPFVMSLDHLGPFARSVEDLALAYDVMQGPDS
DDAACTSRAPEPVSGQLSQGLDGLRIAIAGGHFQKNLFPEAAEAVSRVVAALKVTQVIEVPEAARARAAAYVISTTEGAS
LHLDRLRTRPDDFDPAVRDRLIAGAMIPAPMVDRAQKFRRWYRAKVLELFRSVDVLIAPATPCVAPKLGQATFVLDGVEL
PVRANIGIHTQPISFIGLPVVAVPIPLAPMPIGVQIIAAPWCEHVALRVAHALQQLGVAAAPAPRGVLENGSRSA
>Mature_474_residues
SSPLDSSEMTAGAIAAAVAARKLTAVEATDAALARIAARDGVLNSFTDITAERARAKARAVDADIAAGKPVGPLAGVPFA
VKNLFDVADLPTRAGSKINRDRAPAPRDSTLIERMEAAGAVLVGALNMGEYAYDFTGENIHDGPSRNPHDPTRMSGGSSG
GSGSAVGGGLVPIALGSDTNGSIRVPSSFCGIFGLKPTYGRLSRARSFPFVMSLDHLGPFARSVEDLALAYDVMQGPDSD
DAACTSRAPEPVSGQLSQGLDGLRIAIAGGHFQKNLFPEAAEAVSRVVAALKVTQVIEVPEAARARAAAYVISTTEGASL
HLDRLRTRPDDFDPAVRDRLIAGAMIPAPMVDRAQKFRRWYRAKVLELFRSVDVLIAPATPCVAPKLGQATFVLDGVELP
VRANIGIHTQPISFIGLPVVAVPIPLAPMPIGVQIIAAPWCEHVALRVAHALQQLGVAAAPAPRGVLENGSRSA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=27.1457085828343, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI195972892, Length=240, Percent_Identity=32.9166666666667, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI166795287, Length=461, Percent_Identity=25.5965292841649, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=448, Percent_Identity=29.6875, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17537465, Length=496, Percent_Identity=26.4112903225806, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=223, Percent_Identity=29.1479820627803, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=185, Percent_Identity=30.8108108108108, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=174, Percent_Identity=32.7586206896552, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=300, Percent_Identity=25.6666666666667, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=300, Percent_Identity=26, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=413, Percent_Identity=30.2663438256659, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323950, Length=416, Percent_Identity=24.0384615384615, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320448, Length=240, Percent_Identity=27.5, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648113, Length=497, Percent_Identity=30.1810865191147, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24644968, Length=477, Percent_Identity=26.2054507337526, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24652985, Length=497, Percent_Identity=26.9617706237425, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922090, Length=497, Percent_Identity=26.9617706237425, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652981, Length=497, Percent_Identity=26.9617706237425, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652983, Length=497, Percent_Identity=26.9617706237425, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=26.8, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648435, Length=491, Percent_Identity=22.1995926680244, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648437, Length=491, Percent_Identity=22.1995926680244, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45550774, Length=491, Percent_Identity=22.1995926680244, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356731, Length=238, Percent_Identity=28.9915966386555, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648441, Length=419, Percent_Identity=21.9570405727924, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24648439, Length=419, Percent_Identity=21.9570405727924, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49509; Mature: 49378

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPLDSSEMTAGAIAAAVAARKLTAVEATDAALARIAARDGVLNSFTDITAERARAKAR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AVDADIAAGKPVGPLAGVPFAVKNLFDVADLPTRAGSKINRDRAPAPRDSTLIERMEAAG
HCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
AVLVGALNMGEYAYDFTGENIHDGPSRNPHDPTRMSGGSSGGSGSAVGGGLVPIALGSDT
EEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
NGSIRVPSSFCGIFGLKPTYGRLSRARSFPFVMSLDHLGPFARSVEDLALAYDVMQGPDS
CCCEECCHHHHHHCCCCCCHHHHHHHCCCCEEEEHHHCCHHHHHHHHHHHHHHHHCCCCC
DDAACTSRAPEPVSGQLSQGLDGLRIAIAGGHFQKNLFPEAAEAVSRVVAALKVTQVIEV
CCCHHHCCCCCCCCCHHHCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHC
PEAARARAAAYVISTTEGASLHLDRLRTRPDDFDPAVRDRLIAGAMIPAPMVDRAQKFRR
CHHHHHHHEEEEEEECCCCEEEHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
WYRAKVLELFRSVDVLIAPATPCVAPKLGQATFVLDGVELPVRANIGIHTQPISFIGLPV
HHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEECCCCCEECCCCCEECCHHHHCCCH
VAVPIPLAPMPIGVQIIAAPWCEHVALRVAHALQQLGVAAAPAPRGVLENGSRSA
HEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
SSPLDSSEMTAGAIAAAVAARKLTAVEATDAALARIAARDGVLNSFTDITAERARAKAR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AVDADIAAGKPVGPLAGVPFAVKNLFDVADLPTRAGSKINRDRAPAPRDSTLIERMEAAG
HCCCHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
AVLVGALNMGEYAYDFTGENIHDGPSRNPHDPTRMSGGSSGGSGSAVGGGLVPIALGSDT
EEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
NGSIRVPSSFCGIFGLKPTYGRLSRARSFPFVMSLDHLGPFARSVEDLALAYDVMQGPDS
CCCEECCHHHHHHCCCCCCHHHHHHHCCCCEEEEHHHCCHHHHHHHHHHHHHHHHCCCCC
DDAACTSRAPEPVSGQLSQGLDGLRIAIAGGHFQKNLFPEAAEAVSRVVAALKVTQVIEV
CCCHHHCCCCCCCCCHHHCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHC
PEAARARAAAYVISTTEGASLHLDRLRTRPDDFDPAVRDRLIAGAMIPAPMVDRAQKFRR
CHHHHHHHEEEEEEECCCCEEEHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
WYRAKVLELFRSVDVLIAPATPCVAPKLGQATFVLDGVELPVRANIGIHTQPISFIGLPV
HHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEECCCCCEECCCCCEECCHHHHCCCH
VAVPIPLAPMPIGVQIIAAPWCEHVALRVAHALQQLGVAAAPAPRGVLENGSRSA
HEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA