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The map label for this gene is boxB [H]

Identifier: 146343551

GI number: 146343551

Start: 7046511

End: 7047947

Strand: Reverse

Name: boxB [H]

Synonym: BRADO6788

Alternate gene names: 146343551

Gene position: 7047947-7046511 (Counterclockwise)

Preceding gene: 146343552

Following gene: 146343548

Centisome position: 94.52

GC content: 63.74

Gene sequence:

>1437_bases
ATGAACGTCGACTACTCGACCAAGATTCCCAACAATGTGAATCTCGCCGAGGACCGTCAGGTCCTGAAGGCGCTCGAAGG
CTGGCACCCCGGCTATGTCGACTGGTGGAACGATATGGGCCCAGAGGGCTTCCAGCAGTCACTGGTTTACCTGCGCACGG
CCTATTCGGTCGATCCGCGCGGCTGGGCCAAGTTCGACTATGTCAAGATGCCCGACTATCGCTGGGGCATCCTGCTGGCT
CCCCAGGAAGAGGACCGCAAGATCCCGTTCGGCGAGCATTATGGCGAGCCGGCGTGGCAGGAGGTTCCCGGTGAATATCG
CGCGCTGATGCGCCGGCTGATCGTGATCCAGGGCGACACCGAACCGGCCTCGGTCGAGCAGCAGCGCCATCTCGGCAAGA
CCGCGCCGTCGCTCTACGACATGCGCAATTTGTTCCAGGTCAATGTCGAGGAGGGCCGGCACCTCTGGGCGATGGTCTAT
CTGCTGCAGAAGTACTTCGGCCGCGACGGCCGCGAGGAGGCGGATGAGCTGCTGCGCCGCCGTTCGGGCGATGCCGACTC
TCCGCGCATGCTCGGCGCCTTCAACGAGAAGACGCCGGACTGGCTGTCGTTCTTCATGTTCACCTACTTCACCGACCGCG
ACGGCAAGATGCAGTTGCACTCGCTCGCGCAGTCCGGCTTCGATCCGCTATCGCGCACCTGCCGCTTCATGCTGACCGAA
GAGGCGCACCACATGTTCGTCGGCGAGACCGGCGTCGGCCGCGTCATCCAGCGCACCGTCGATGCGATGCGGGAGGCCGG
CATCGACGATCCCACCGACATCGCCAAGGTCCGCGCGCTCGGCGTCATCGACCTGCCGACCATCCAGAAGAAGCTCAACC
TGCACTACACGCTGTCGCTGGATCTGTTCGGCTCCGAGGTCTCGACCAACGCGGCCAACGCCTTCAATGCCGGCATCAAG
GGCCGCTATCACGAGACCCAGATCCAGGACGACCACCAGCTCAAGAACTCCACCTATCCGGTGCTCAAGCTGGTCGATGG
CGAGATCAAGCGCGTCGACGAGCCGGCGCTGACCGCGCTCAACATGCGCCTGCGCGACGACTACTCGCAGGACTGCGTCA
AGGGCCTGTTGCGCTGGAACAAGATCATCAGCACCGCCGGCTACGACTTCCAGCTCAAGCTGCCGCACACCGCCTTCCAC
CGCGCGATCGGCGAGTTCAAGGATGTGCATGCCACGCCCGACGGCATCCTGATCGACGAGGCCACCTGGCAGAAGCGCAA
GGGCGACTGGCTGCCGTCGACCGATGACGGCGCCTACATCACCTCGCTGATGAAACCGGTGACGGAGATCGGCGAGTACG
CCTCCTGGATCGCCCCGCCGAAGGTCGGCATCGACAACAAGCCGGGCGACTTCGAGTACGTGAAGATCGAGACCTGA

Upstream 100 bases:

>100_bases
ATCCACGAGCCAAGCAGCTCGCACGACGTGGATGCCCGGAACAAGGCCGGGCATGACCACCGGAAAGGTTTCAGGGAGGC
ACGCCATGAACATGAACATC

Downstream 100 bases:

>100_bases
GCTCGATCACCTCGCGCAGTCCTAAAACCGGTGTCGTCCCGGCCTTGAGCCGGGAGGCATACCGCGTGATGTGGCGGTGA
GAGCAAAGTGGCCGCCGCCG

Product: benzoyl-CoA oxygenase BoxB

Products: NA

Alternate protein names: Benzoyl-CoA 2,3-dioxygenase subunit B; Benzoyl-CoA dioxygenase oxygenase component [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNVDYSTKIPNNVNLAEDRQVLKALEGWHPGYVDWWNDMGPEGFQQSLVYLRTAYSVDPRGWAKFDYVKMPDYRWGILLA
PQEEDRKIPFGEHYGEPAWQEVPGEYRALMRRLIVIQGDTEPASVEQQRHLGKTAPSLYDMRNLFQVNVEEGRHLWAMVY
LLQKYFGRDGREEADELLRRRSGDADSPRMLGAFNEKTPDWLSFFMFTYFTDRDGKMQLHSLAQSGFDPLSRTCRFMLTE
EAHHMFVGETGVGRVIQRTVDAMREAGIDDPTDIAKVRALGVIDLPTIQKKLNLHYTLSLDLFGSEVSTNAANAFNAGIK
GRYHETQIQDDHQLKNSTYPVLKLVDGEIKRVDEPALTALNMRLRDDYSQDCVKGLLRWNKIISTAGYDFQLKLPHTAFH
RAIGEFKDVHATPDGILIDEATWQKRKGDWLPSTDDGAYITSLMKPVTEIGEYASWIAPPKVGIDNKPGDFEYVKIET

Sequences:

>Translated_478_residues
MNVDYSTKIPNNVNLAEDRQVLKALEGWHPGYVDWWNDMGPEGFQQSLVYLRTAYSVDPRGWAKFDYVKMPDYRWGILLA
PQEEDRKIPFGEHYGEPAWQEVPGEYRALMRRLIVIQGDTEPASVEQQRHLGKTAPSLYDMRNLFQVNVEEGRHLWAMVY
LLQKYFGRDGREEADELLRRRSGDADSPRMLGAFNEKTPDWLSFFMFTYFTDRDGKMQLHSLAQSGFDPLSRTCRFMLTE
EAHHMFVGETGVGRVIQRTVDAMREAGIDDPTDIAKVRALGVIDLPTIQKKLNLHYTLSLDLFGSEVSTNAANAFNAGIK
GRYHETQIQDDHQLKNSTYPVLKLVDGEIKRVDEPALTALNMRLRDDYSQDCVKGLLRWNKIISTAGYDFQLKLPHTAFH
RAIGEFKDVHATPDGILIDEATWQKRKGDWLPSTDDGAYITSLMKPVTEIGEYASWIAPPKVGIDNKPGDFEYVKIET
>Mature_478_residues
MNVDYSTKIPNNVNLAEDRQVLKALEGWHPGYVDWWNDMGPEGFQQSLVYLRTAYSVDPRGWAKFDYVKMPDYRWGILLA
PQEEDRKIPFGEHYGEPAWQEVPGEYRALMRRLIVIQGDTEPASVEQQRHLGKTAPSLYDMRNLFQVNVEEGRHLWAMVY
LLQKYFGRDGREEADELLRRRSGDADSPRMLGAFNEKTPDWLSFFMFTYFTDRDGKMQLHSLAQSGFDPLSRTCRFMLTE
EAHHMFVGETGVGRVIQRTVDAMREAGIDDPTDIAKVRALGVIDLPTIQKKLNLHYTLSLDLFGSEVSTNAANAFNAGIK
GRYHETQIQDDHQLKNSTYPVLKLVDGEIKRVDEPALTALNMRLRDDYSQDCVKGLLRWNKIISTAGYDFQLKLPHTAFH
RAIGEFKDVHATPDGILIDEATWQKRKGDWLPSTDDGAYITSLMKPVTEIGEYASWIAPPKVGIDNKPGDFEYVKIET

Specific function: The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-dihydro-2,3-dihydroxybenzoyl-CoA. BoxB acts as the benzoyl-CoA oxygenase, after being reduced by the reductase component BoxA. BoxAB does not

COG id: COG3396

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the benzoyl-CoA oxygenase component B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017635
- InterPro:   IPR012347
- InterPro:   IPR009078
- InterPro:   IPR007814 [H]

Pfam domain/function: PF05138 PaaA_PaaC [H]

EC number: =1.14.12.21 [H]

Molecular weight: Translated: 54749; Mature: 54749

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVDYSTKIPNNVNLAEDRQVLKALEGWHPGYVDWWNDMGPEGFQQSLVYLRTAYSVDPR
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
GWAKFDYVKMPDYRWGILLAPQEEDRKIPFGEHYGEPAWQEVPGEYRALMRRLIVIQGDT
CCCEECEEECCCCCEEEEECCCCCCCCCCCHHHCCCCHHHHCCHHHHHHHHHHEEEECCC
EPASVEQQRHLGKTAPSLYDMRNLFQVNVEEGRHLWAMVYLLQKYFGRDGREEADELLRR
CCCHHHHHHHCCCCCCHHHHHHHHHEEEHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RSGDADSPRMLGAFNEKTPDWLSFFMFTYFTDRDGKMQLHSLAQSGFDPLSRTCRFMLTE
HCCCCCCCCEECCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHCCCCHHHHHHHHHHHH
EAHHMFVGETGVGRVIQRTVDAMREAGIDDPTDIAKVRALGVIDLPTIQKKLNLHYTLSL
CCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCCEEEEEEE
DLFGSEVSTNAANAFNAGIKGRYHETQIQDDHQLKNSTYPVLKLVDGEIKRVDEPALTAL
EECCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHCCCCCCEEEECCCHHHHCCCHHHHHH
NMRLRDDYSQDCVKGLLRWNKIISTAGYDFQLKLPHTAFHRAIGEFKDVHATPDGILIDE
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEEC
ATWQKRKGDWLPSTDDGAYITSLMKPVTEIGEYASWIAPPKVGIDNKPGDFEYVKIET
CHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MNVDYSTKIPNNVNLAEDRQVLKALEGWHPGYVDWWNDMGPEGFQQSLVYLRTAYSVDPR
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
GWAKFDYVKMPDYRWGILLAPQEEDRKIPFGEHYGEPAWQEVPGEYRALMRRLIVIQGDT
CCCEECEEECCCCCEEEEECCCCCCCCCCCHHHCCCCHHHHCCHHHHHHHHHHEEEECCC
EPASVEQQRHLGKTAPSLYDMRNLFQVNVEEGRHLWAMVYLLQKYFGRDGREEADELLRR
CCCHHHHHHHCCCCCCHHHHHHHHHEEEHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RSGDADSPRMLGAFNEKTPDWLSFFMFTYFTDRDGKMQLHSLAQSGFDPLSRTCRFMLTE
HCCCCCCCCEECCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHCCCCHHHHHHHHHHHH
EAHHMFVGETGVGRVIQRTVDAMREAGIDDPTDIAKVRALGVIDLPTIQKKLNLHYTLSL
CCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCCEEEEEEE
DLFGSEVSTNAANAFNAGIKGRYHETQIQDDHQLKNSTYPVLKLVDGEIKRVDEPALTAL
EECCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHCCCCCCEEEECCCHHHHCCCHHHHHH
NMRLRDDYSQDCVKGLLRWNKIISTAGYDFQLKLPHTAFHRAIGEFKDVHATPDGILIDE
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCEEEEC
ATWQKRKGDWLPSTDDGAYITSLMKPVTEIGEYASWIAPPKVGIDNKPGDFEYVKIET
CHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11222587; 12399500 [H]