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The map label for this gene is lysK [H]

Identifier: 146343513

GI number: 146343513

Start: 7006474

End: 7008120

Strand: Reverse

Name: lysK [H]

Synonym: BRADO6745

Alternate gene names: 146343513

Gene position: 7008120-7006474 (Counterclockwise)

Preceding gene: 146343514

Following gene: 146343511

Centisome position: 93.99

GC content: 64.97

Gene sequence:

>1647_bases
ATGTCCACGATCGATCTCGCACCGACCAGCCCGAGCGACCTGCGCGCGCTCGCCGAGAATTCAAACGCCTGGCCGTTCGA
GCAGGCGAAGGCGATCGTCAACCGGCTGAAGAAGGCGCCGAAGGACGAGGTGCTGTTCGAGACCGGCTACGGACCGTCCG
GCCTGCCGCATATCGGCACCTTCGGCGAGGTCGCGCGCACCACCATGGTGCGCCACGCCTTCCGCGTGCTCACCGAGGAC
AAGATCAAGACGCGCCTCATCGCCTTCTCCGACGACATGGACGGCCTGCGCAAGGTGCCGGACAACGTGCCGAACAAGGA
GATGCTGGCGAGCCATCTGGGCAAGCCGCTGTCGCGCGTCCCGGATCCGTTCTCCAACGAATATCCGTCGTTTGCCGCGC
ACAACAATGCCCGGCTGCGCGCGTTCCTCGACACGTTCGGCTTCGACTACGAGTTCGCCTCCTCGACCGAGTACTACACC
TCCGGCAAGTTCGACGCGGCGCTGCTGCGCATGCTGGAGCGGCTGGAGAAGGTGATGGCGATCATGCTGCCGTCGCTCCG
CGAGGAGCGTGCGGCCAGCTACTCGCCGTTCCTGCCCATCTGCCCGCGCACCGGCCTCGTGCTGTACGTGCCGATCGTCG
AGCACGACGCCAAGGCCGGCACGATCTCCTATGACGATCCCGAGACCAAGGAGCGCATGACCGTTCCGGTCACCGGCGGC
CATTGCAAGCTGCAATGGAAGCCGGACTGGGCGATGCGCTGGTTCGCGCTCGGCGTCGACTATGAAATGGCCGGCAAGGA
CCTGATCGATTCGGTGAAGCTGTCGGGCAAGATCTGCGCGGCGCTCGGCGGCACGCCGCCGGAGGGCTTCAACTACGAAC
TGTTCCTCGACGAGAAGGGCCAGAAGATCTCGAAGTCGAAGGGCAACGGCCTGACCATCGACGAATGGCTGCGCTACGCC
TCGCCGGAATCGCTGTCGCTGTTCATGTATCGCGAGCCCAAGGCGGCGAAGCGGCTGTATTTCGACGTCATCCCGCGCAA
CGTCGACGACTATCAGCAGTTCCTGGAAGGTTTTCCGCGGCAGGATGCCAAGCAGCAGCTCGGCAATCCCGTCTGGCACA
TCCATTCCGGCAAGCCGCCGAAGGCTGACATGCCGGTGACGTTCCAGTTGCTGCTGACCTTGGTGTCGTCGTCGAACGCG
GAGAATGCCGAGACGCTGTGGGGCTTCATCGGCCGCTACCGGCCGGGCGTGACGCCGCAGACGCATCCGAAGCTCGATGC
GATGGTCGGCTACGCCATCAACTACTATCGCGACTTCGTCGCGCCGACCAAGACGTTCCGCGAGCCGACCGAGGGCGAAC
GCGTGGCGCTGCAGGATCTGCGCGACGCATTGTCCAACCTGCCGGCAGAGGCAGCGGCCGAGGACATCCAGAACGTGGTC
TACGAGATCGGCCGGCGCGAGCCGTTCCTCGACCACGTCAAGAAGGGCAAGGATGGCCGGCCCGGCGTGTCGCTGGATTG
GTTCAACATGCTCTACCAGGTGCTGCTCGGCCAGGAGAAGGGCCCGCGCTTCGGCTCCTTCGTCGCGGTGTATGGCATCG
GCAATGCGGTCAGCATGATCGACGGCGCGCTGGCGCGTTCGGCGTGA

Upstream 100 bases:

>100_bases
GACGAAGCAATCCAGAGCCGCGCGTGAGACTCTGGATCGCTTCGCTTCGCTCGCGATGACGCCTTGTGTCAATTCTGCTG
CTTTGAACCGGTACTGTCAG

Downstream 100 bases:

>100_bases
GGCCCACGCTGGTCATTACACAAACGGTGTCGTCCCGGCCTCCGAGCCGGGACCCATGACCACCGGATCGAGTTTGGCGG
AGGATCTCCGCCACCTCGCA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSTIDLAPTSPSDLRALAENSNAWPFEQAKAIVNRLKKAPKDEVLFETGYGPSGLPHIGTFGEVARTTMVRHAFRVLTED
KIKTRLIAFSDDMDGLRKVPDNVPNKEMLASHLGKPLSRVPDPFSNEYPSFAAHNNARLRAFLDTFGFDYEFASSTEYYT
SGKFDAALLRMLERLEKVMAIMLPSLREERAASYSPFLPICPRTGLVLYVPIVEHDAKAGTISYDDPETKERMTVPVTGG
HCKLQWKPDWAMRWFALGVDYEMAGKDLIDSVKLSGKICAALGGTPPEGFNYELFLDEKGQKISKSKGNGLTIDEWLRYA
SPESLSLFMYREPKAAKRLYFDVIPRNVDDYQQFLEGFPRQDAKQQLGNPVWHIHSGKPPKADMPVTFQLLLTLVSSSNA
ENAETLWGFIGRYRPGVTPQTHPKLDAMVGYAINYYRDFVAPTKTFREPTEGERVALQDLRDALSNLPAEAAAEDIQNVV
YEIGRREPFLDHVKKGKDGRPGVSLDWFNMLYQVLLGQEKGPRFGSFVAVYGIGNAVSMIDGALARSA

Sequences:

>Translated_548_residues
MSTIDLAPTSPSDLRALAENSNAWPFEQAKAIVNRLKKAPKDEVLFETGYGPSGLPHIGTFGEVARTTMVRHAFRVLTED
KIKTRLIAFSDDMDGLRKVPDNVPNKEMLASHLGKPLSRVPDPFSNEYPSFAAHNNARLRAFLDTFGFDYEFASSTEYYT
SGKFDAALLRMLERLEKVMAIMLPSLREERAASYSPFLPICPRTGLVLYVPIVEHDAKAGTISYDDPETKERMTVPVTGG
HCKLQWKPDWAMRWFALGVDYEMAGKDLIDSVKLSGKICAALGGTPPEGFNYELFLDEKGQKISKSKGNGLTIDEWLRYA
SPESLSLFMYREPKAAKRLYFDVIPRNVDDYQQFLEGFPRQDAKQQLGNPVWHIHSGKPPKADMPVTFQLLLTLVSSSNA
ENAETLWGFIGRYRPGVTPQTHPKLDAMVGYAINYYRDFVAPTKTFREPTEGERVALQDLRDALSNLPAEAAAEDIQNVV
YEIGRREPFLDHVKKGKDGRPGVSLDWFNMLYQVLLGQEKGPRFGSFVAVYGIGNAVSMIDGALARSA
>Mature_547_residues
STIDLAPTSPSDLRALAENSNAWPFEQAKAIVNRLKKAPKDEVLFETGYGPSGLPHIGTFGEVARTTMVRHAFRVLTEDK
IKTRLIAFSDDMDGLRKVPDNVPNKEMLASHLGKPLSRVPDPFSNEYPSFAAHNNARLRAFLDTFGFDYEFASSTEYYTS
GKFDAALLRMLERLEKVMAIMLPSLREERAASYSPFLPICPRTGLVLYVPIVEHDAKAGTISYDDPETKERMTVPVTGGH
CKLQWKPDWAMRWFALGVDYEMAGKDLIDSVKLSGKICAALGGTPPEGFNYELFLDEKGQKISKSKGNGLTIDEWLRYAS
PESLSLFMYREPKAAKRLYFDVIPRNVDDYQQFLEGFPRQDAKQQLGNPVWHIHSGKPPKADMPVTFQLLLTLVSSSNAE
NAETLWGFIGRYRPGVTPQTHPKLDAMVGYAINYYRDFVAPTKTFREPTEGERVALQDLRDALSNLPAEAAAEDIQNVVY
EIGRREPFLDHVKKGKDGRPGVSLDWFNMLYQVLLGQEKGPRFGSFVAVYGIGNAVSMIDGALARSA

Specific function: Unknown

COG id: COG1384

COG function: function code J; Lysyl-tRNA synthetase (class I)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR002904
- InterPro:   IPR020754
- InterPro:   IPR020756
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01921 tRNA-synt_1f [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 61297; Mature: 61166

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIDLAPTSPSDLRALAENSNAWPFEQAKAIVNRLKKAPKDEVLFETGYGPSGLPHIGT
CCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
FGEVARTTMVRHAFRVLTEDKIKTRLIAFSDDMDGLRKVPDNVPNKEMLASHLGKPLSRV
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHCCCCCCCHHHHHHHHCCHHHHC
PDPFSNEYPSFAAHNNARLRAFLDTFGFDYEFASSTEYYTSGKFDAALLRMLERLEKVMA
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
IMLPSLREERAASYSPFLPICPRTGLVLYVPIVEHDAKAGTISYDDPETKERMTVPVTGG
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECC
HCKLQWKPDWAMRWFALGVDYEMAGKDLIDSVKLSGKICAALGGTPPEGFNYELFLDEKG
EEEEEECCCHHEEEEEECCCHHHCCHHHHHHHHHCCHHHEECCCCCCCCCCEEEEECCCC
QKISKSKGNGLTIDEWLRYASPESLSLFMYREPKAAKRLYFDVIPRNVDDYQQFLEGFPR
CHHHCCCCCCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHCCCC
QDAKQQLGNPVWHIHSGKPPKADMPVTFQLLLTLVSSSNAENAETLWGFIGRYRPGVTPQ
CHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCC
THPKLDAMVGYAINYYRDFVAPTKTFREPTEGERVALQDLRDALSNLPAEAAAEDIQNVV
CCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
YEIGRREPFLDHVKKGKDGRPGVSLDWFNMLYQVLLGQEKGPRFGSFVAVYGIGNAVSMI
HHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHH
DGALARSA
HHHHHCCC
>Mature Secondary Structure 
STIDLAPTSPSDLRALAENSNAWPFEQAKAIVNRLKKAPKDEVLFETGYGPSGLPHIGT
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
FGEVARTTMVRHAFRVLTEDKIKTRLIAFSDDMDGLRKVPDNVPNKEMLASHLGKPLSRV
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHCCCCCCCHHHHHHHHCCHHHHC
PDPFSNEYPSFAAHNNARLRAFLDTFGFDYEFASSTEYYTSGKFDAALLRMLERLEKVMA
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
IMLPSLREERAASYSPFLPICPRTGLVLYVPIVEHDAKAGTISYDDPETKERMTVPVTGG
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECC
HCKLQWKPDWAMRWFALGVDYEMAGKDLIDSVKLSGKICAALGGTPPEGFNYELFLDEKG
EEEEEECCCHHEEEEEECCCHHHCCHHHHHHHHHCCHHHEECCCCCCCCCCEEEEECCCC
QKISKSKGNGLTIDEWLRYASPESLSLFMYREPKAAKRLYFDVIPRNVDDYQQFLEGFPR
CHHHCCCCCCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHCCCC
QDAKQQLGNPVWHIHSGKPPKADMPVTFQLLLTLVSSSNAENAETLWGFIGRYRPGVTPQ
CHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCC
THPKLDAMVGYAINYYRDFVAPTKTFREPTEGERVALQDLRDALSNLPAEAAAEDIQNVV
CCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
YEIGRREPFLDHVKKGKDGRPGVSLDWFNMLYQVLLGQEKGPRFGSFVAVYGIGNAVSMI
HHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHH
DGALARSA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]