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The map label for this gene is rpfC [H]

Identifier: 146343276

GI number: 146343276

Start: 6744404

End: 6745558

Strand: Reverse

Name: rpfC [H]

Synonym: BRADO6496

Alternate gene names: 146343276

Gene position: 6745558-6744404 (Counterclockwise)

Preceding gene: 146343277

Following gene: 146343274

Centisome position: 90.46

GC content: 69.52

Gene sequence:

>1155_bases
TTGGCGGTGTTCGCCCACGAGGTGCGGACGCCCCTGACAGGCATTCTCGCGATCAGCGATCTGCTCGCCACCTCCGAGCT
CGGCGAGCGCGAGCGGCGCTGGGTCGATACGATCAAGGCGGGTGCCGAGCATCTGTCGAGCCTGGCGACGCTGTTCGTCG
ACGCCGCCAAGCACGAGAGCACTGGTGCGATCCTGCGCGAGGATCTGTTCGACCTGCGCGTGCTGGCGCGCGCAGCTGGC
GATTCATTGTCTGGACGGGCGGCGGCGAAGGGGCTGGAGGCGCGTGTCGAGGTCTCCGACGCATTGCCGGGGCTTGTGGT
CGGCGACCCCGTGCGCCTGCGTGCCGCGCTCGAAAACCTGATCGACAATGCGGTGAAGTTCACCGAGCAGGGCGGCGTGG
CGCTTGAGATCGCCCTGGCGGGCAAGGCGGGCCGCGGCAAGGCTGGCAGAGCGGCGCCGAAGGGAACGCCAAAGGGCCGG
GTCGAGGTCGCGTTCCACGTCTCGGATTCCGGCATCGGGTTGAGCCTGCCGGAGATCAAGCGGCTGTTCCGCCCGTTCAG
CCAGGCCAATGTTTCGATCGCCTCGCGTTTCGGCGGCGCTGGTCTCGGCTTGTCCTCGGTGCGGCAACTGGCGCGTGCGA
TGGGGGGAGACGTGACGGTCACCCCGTATGAGGGTGGCGGGACGACCTTCACGCTCACCGTGGCGCTGACGGTGGCGGCT
GCGCCGCACACGACGGCGGCAGGAGGCGAGGATGGCGTGACGCTCGCGCCGGTCAGGCCGCTGCGCCTGCTGGGCGTCGA
GGACAATCCGTTTGGCCGTGTCGTGCTCAACACCATCCTGAACGAGCTCGGTCACCAGGCCGAGTTCATCGGCCGCGGCG
AAGCGGCGCCGGAGCGCATCGCGTCGGGCAGCTACGATGCCGTGCTGATGGACATGGTGCTCCCGGGCATCAACGGCGTG
GAGGCGATCGCGCGGATTCGCGCCCTCGGTGCGCCTCATGGCGACATCCCGATCATCGGCGTCTCCGGGCGGGGCGAGGA
CGAAGCGGCGTCGCGCACCGCCGGCGCGGATGCGTTCCTGCTCAAGCCCGTTTCGCCGAGGGCTCTAGCGACTGCGCTTC
TTGCAGCGACACACCGCGCGGCAGCCGCGACTTGA

Upstream 100 bases:

>100_bases
GCGAGTCGGGGCCAAGCCCCGCGCGGCGAAGGCCGTGGCAGGTCAGCGGGCGCGAAGGAATGACGCGGGGGCGCCAGGAC
CAGGCGTCGTCGAAGCCGCG

Downstream 100 bases:

>100_bases
TGATCGACGCGTTCAACTCGCCGCCGTAGACGAAGATCGCCGCGATGAAATACAGGAACACCAGCGCGATGATCACCGAG
GCGAGCCCTGCATACATCGT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MAVFAHEVRTPLTGILAISDLLATSELGERERRWVDTIKAGAEHLSSLATLFVDAAKHESTGAILREDLFDLRVLARAAG
DSLSGRAAAKGLEARVEVSDALPGLVVGDPVRLRAALENLIDNAVKFTEQGGVALEIALAGKAGRGKAGRAAPKGTPKGR
VEVAFHVSDSGIGLSLPEIKRLFRPFSQANVSIASRFGGAGLGLSSVRQLARAMGGDVTVTPYEGGGTTFTLTVALTVAA
APHTTAAGGEDGVTLAPVRPLRLLGVEDNPFGRVVLNTILNELGHQAEFIGRGEAAPERIASGSYDAVLMDMVLPGINGV
EAIARIRALGAPHGDIPIIGVSGRGEDEAASRTAGADAFLLKPVSPRALATALLAATHRAAAAT

Sequences:

>Translated_384_residues
MAVFAHEVRTPLTGILAISDLLATSELGERERRWVDTIKAGAEHLSSLATLFVDAAKHESTGAILREDLFDLRVLARAAG
DSLSGRAAAKGLEARVEVSDALPGLVVGDPVRLRAALENLIDNAVKFTEQGGVALEIALAGKAGRGKAGRAAPKGTPKGR
VEVAFHVSDSGIGLSLPEIKRLFRPFSQANVSIASRFGGAGLGLSSVRQLARAMGGDVTVTPYEGGGTTFTLTVALTVAA
APHTTAAGGEDGVTLAPVRPLRLLGVEDNPFGRVVLNTILNELGHQAEFIGRGEAAPERIASGSYDAVLMDMVLPGINGV
EAIARIRALGAPHGDIPIIGVSGRGEDEAASRTAGADAFLLKPVSPRALATALLAATHRAAAAT
>Mature_383_residues
AVFAHEVRTPLTGILAISDLLATSELGERERRWVDTIKAGAEHLSSLATLFVDAAKHESTGAILREDLFDLRVLARAAGD
SLSGRAAAKGLEARVEVSDALPGLVVGDPVRLRAALENLIDNAVKFTEQGGVALEIALAGKAGRGKAGRAAPKGTPKGRV
EVAFHVSDSGIGLSLPEIKRLFRPFSQANVSIASRFGGAGLGLSSVRQLARAMGGDVTVTPYEGGGTTFTLTVALTVAAA
PHTTAAGGEDGVTLAPVRPLRLLGVEDNPFGRVVLNTILNELGHQAEFIGRGEAAPERIASGSYDAVLMDMVLPGINGVE
AIARIRALGAPHGDIPIIGVSGRGEDEAASRTAGADAFLLKPVSPRALATALLAATHRAAAAT

Specific function: Involved in the positive regulation of synthesis of extracellular enzymes and polysaccharide, and hence in pathogenicity. Can restore function to the non-pathogenic mutant 8237 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=378, Percent_Identity=33.5978835978836, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788713, Length=388, Percent_Identity=29.1237113402062, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789149, Length=389, Percent_Identity=27.2493573264781, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87081816, Length=386, Percent_Identity=29.7927461139896, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI145693157, Length=252, Percent_Identity=28.968253968254, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=26.1603375527426, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39476; Mature: 39344

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFAHEVRTPLTGILAISDLLATSELGERERRWVDTIKAGAEHLSSLATLFVDAAKHES
CCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TGAILREDLFDLRVLARAAGDSLSGRAAAKGLEARVEVSDALPGLVVGDPVRLRAALENL
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHEEEHHHCCCCEEECCHHHHHHHHHHH
IDNAVKFTEQGGVALEIALAGKAGRGKAGRAAPKGTPKGRVEVAFHVSDSGIGLSLPEIK
HHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHH
RLFRPFSQANVSIASRFGGAGLGLSSVRQLARAMGGDVTVTPYEGGGTTFTLTVALTVAA
HHHCCHHHCCHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEEEC
APHTTAAGGEDGVTLAPVRPLRLLGVEDNPFGRVVLNTILNELGHQAEFIGRGEAAPERI
CCCCCCCCCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHH
ASGSYDAVLMDMVLPGINGVEAIARIRALGAPHGDIPIIGVSGRGEDEAASRTAGADAFL
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCEEE
LKPVSPRALATALLAATHRAAAAT
ECCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVFAHEVRTPLTGILAISDLLATSELGERERRWVDTIKAGAEHLSSLATLFVDAAKHES
CEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TGAILREDLFDLRVLARAAGDSLSGRAAAKGLEARVEVSDALPGLVVGDPVRLRAALENL
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHEEEHHHCCCCEEECCHHHHHHHHHHH
IDNAVKFTEQGGVALEIALAGKAGRGKAGRAAPKGTPKGRVEVAFHVSDSGIGLSLPEIK
HHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHH
RLFRPFSQANVSIASRFGGAGLGLSSVRQLARAMGGDVTVTPYEGGGTTFTLTVALTVAA
HHHCCHHHCCHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEEEC
APHTTAAGGEDGVTLAPVRPLRLLGVEDNPFGRVVLNTILNELGHQAEFIGRGEAAPERI
CCCCCCCCCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHH
ASGSYDAVLMDMVLPGINGVEAIARIRALGAPHGDIPIIGVSGRGEDEAASRTAGADAFL
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCEEE
LKPVSPRALATALLAATHRAAAAT
ECCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1645442 [H]