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The map label for this gene is ogt [H]

Identifier: 146343220

GI number: 146343220

Start: 6682857

End: 6683444

Strand: Reverse

Name: ogt [H]

Synonym: BRADO6430

Alternate gene names: 146343220

Gene position: 6683444-6682857 (Counterclockwise)

Preceding gene: 146343224

Following gene: 146343215

Centisome position: 89.63

GC content: 67.18

Gene sequence:

>588_bases
ATGACAGACCACTTCATGACAGACCAGTTGATGTCAGACCAGTTCATGACAGACCAGAGCTACGCCATGTTCGAGACCGC
GATCGGCACCTGCGCGATCGCCTGGGGCCCGCGCGGCATCAACGCGGTGCAGCTGCCGATGGGCGGCGTGGCGCAGCTCC
GCACGCGGATGCAGCAGCGCTCCGCCGGCATTGACGAGGCTGAGCCGCCGCAGCATGTGCAGCGCGTCATCAGCCGCATC
ACGGCGTTGCTGGCCGGAGAGCCCGATGACCTCACCGACATCGATCTCGACCTCGACGGTGTCAGCGCCTTCCAGCGCGG
CGTCTACGCCATTGCGCGCCGCATCCCGCCCGGCCGGACCTTGACCTATGGCGACATCGCCAAGCAGCTTGGCGGCGTCG
AGCTGTCGCGCGAGGTCGGCCAGGCGCTCGGGCGCAACCCGTGCCCGATCGTGGTGCCCTGTCATCGCGTGCTGGCGGCA
GGCAACAAGCCGGGCGGCTTCTCCGCCAATGGCGGCGTCGATACCAAGCTGAAGATGCTGGCGATCGAGGGCGCGGCGGT
GAACCACCAGCCCAGCCTATTCGATTAA

Upstream 100 bases:

>100_bases
CGGCGGCTGTCGACATTGGCAGAATCCGGGTGGATCGCAACATTCTCCAAGCCTATTTTGACCGGCAGATGATACGGGGC
AGAGCGACGAGGAGCCCGCC

Downstream 100 bases:

>100_bases
AGGCGCAGCTCGGAGGCTCACTTAGCTGTCGTCACCCGCGAAAGCGGGTGACCCAGTATTCCACCGGCGGTCGTTTTCTG
CGGAAAGTTCACGGCGTACT

Product: putative methylated-DNA-protein-cystein methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MTDHFMTDQLMSDQFMTDQSYAMFETAIGTCAIAWGPRGINAVQLPMGGVAQLRTRMQQRSAGIDEAEPPQHVQRVISRI
TALLAGEPDDLTDIDLDLDGVSAFQRGVYAIARRIPPGRTLTYGDIAKQLGGVELSREVGQALGRNPCPIVVPCHRVLAA
GNKPGGFSANGGVDTKLKMLAIEGAAVNHQPSLFD

Sequences:

>Translated_195_residues
MTDHFMTDQLMSDQFMTDQSYAMFETAIGTCAIAWGPRGINAVQLPMGGVAQLRTRMQQRSAGIDEAEPPQHVQRVISRI
TALLAGEPDDLTDIDLDLDGVSAFQRGVYAIARRIPPGRTLTYGDIAKQLGGVELSREVGQALGRNPCPIVVPCHRVLAA
GNKPGGFSANGGVDTKLKMLAIEGAAVNHQPSLFD
>Mature_194_residues
TDHFMTDQLMSDQFMTDQSYAMFETAIGTCAIAWGPRGINAVQLPMGGVAQLRTRMQQRSAGIDEAEPPQHVQRVISRIT
ALLAGEPDDLTDIDLDLDGVSAFQRGVYAIARRIPPGRTLTYGDIAKQLGGVELSREVGQALGRNPCPIVVPCHRVLAAG
NKPGGFSANGGVDTKLKMLAIEGAAVNHQPSLFD

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=168, Percent_Identity=31.547619047619, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787596, Length=85, Percent_Identity=44.7058823529412, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20901; Mature: 20770

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHFMTDQLMSDQFMTDQSYAMFETAIGTCAIAWGPRGINAVQLPMGGVAQLRTRMQQR
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEECCCCCCEEECCCCCHHHHHHHHHHH
SAGIDEAEPPQHVQRVISRITALLAGEPDDLTDIDLDLDGVSAFQRGVYAIARRIPPGRT
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCE
LTYGDIAKQLGGVELSREVGQALGRNPCPIVVPCHRVLAAGNKPGGFSANGGVDTKLKML
EEHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCCCCCCCCCCCCEEEEE
AIEGAAVNHQPSLFD
EEECCCCCCCCCCCC
>Mature Secondary Structure 
TDHFMTDQLMSDQFMTDQSYAMFETAIGTCAIAWGPRGINAVQLPMGGVAQLRTRMQQR
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEECCCCCCEEECCCCCHHHHHHHHHHH
SAGIDEAEPPQHVQRVISRITALLAGEPDDLTDIDLDLDGVSAFQRGVYAIARRIPPGRT
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCE
LTYGDIAKQLGGVELSREVGQALGRNPCPIVVPCHRVLAAGNKPGGFSANGGVDTKLKML
EEHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCCCCCCCCCCCCEEEEE
AIEGAAVNHQPSLFD
EEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]