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The map label for this gene is rep [H]

Identifier: 146343178

GI number: 146343178

Start: 6638713

End: 6639930

Strand: Direct

Name: rep [H]

Synonym: BRADO6379

Alternate gene names: 146343178

Gene position: 6638713-6639930 (Clockwise)

Preceding gene: 146343177

Following gene: 146343179

Centisome position: 89.03

GC content: 62.73

Gene sequence:

>1218_bases
ATGCTCTCCGAGCCCAGCATCGACCCGCTCCAGCACGAATGGATGGGAGCTGCGAGCAGCCAAGTTCTGCTCGGCCTCGA
TGCTCGACCGGATTTCATCTGTGAGCAGATCGACCCGCCCGTAGATTTACGGGATCGGGACAATCTGCCCGACGTCGGCA
AACGACTTGGCAAGGTCGGACGGCGCGACTTCCAACGCCTTCAGCAGGAAAAGCTCGCAATCGCCGAGAGCCTGAAGGAA
GCCGGCCGCGACGAAGACGCCGGCCTGCTCCGAAACTGCTGCACCAACCTCATCATCGATAAGTACGAGAAGGGTCGCCG
GGTACGCGGCCCCAATGAATGCAAGCACCCTCTGTGCCCGACCGCGCAATTGGCACGCCAGCGGCGGACCTATCGACGCG
TCGCACAGGCATTGGAGCGCTACCTGCGACGCAAAGAGGGAATGGTGGGCATCATGCTTACCACCACCATGGAGAGCTGC
CCGTCGCATGAACTGCGCTACCGCACGGGCCAGCTGATCGCAGCCTGTTCACGGCTCATGACCTTTGCCCCGGTCAAGCG
GGCGGTCGCCGCCTACGTCAGGGTCATTGAATTCACCCGCAACGCGGTGACACAGCTGTGGCACCCTCATGCCCATTGGC
TTCTGCTGGTGCCGAAGTCCTACTTTGCCAAGGGCAGCAAGACCTTCATCGACCATGACCGATGGAGCAAGCTGTTCCGC
AAAGCGCTGCGCTGCGCGTACACGCCTATCGTTGATGTCCGTGTCGCGAAAGGGGTGTCGTCCCCGCTTTCCGAGGCGGG
ACTCAAGCCCCTCCGTGAGATCGCCAAGTACACCACCAAGCCCGGCACGCTGGTCTACTGGGACAACGGCATGCCGTACG
CGGTCGGCCGGCAACGGCCTGAGCTGTATCGCAAAGCCAAGGGCGCGCCGCTAGAGCCGATGTTCAACGTGCCGGTGATG
GCGCTGGCAGACGCCGTCAAAGGCCGCCGCCTGGTCGCTCTGTCGCGCAATCTCGAAACCGACGAAGAGCTGGATTTCAC
GGACGACCCTCGCGGCGACGAGCTTGATCAGACCACCGAAGATCTCGGCGCCTTCATCTGCACCGAGTTCTACGAGTGGC
GCGGTCGCGGGCGGCACGGCGATTACTTTCTCGTCGGTCGCTCGTTCGACGAGCCCGGTCAACACAAACGCAAGGAGGGA
TTCGCAATGGGGCCGTGA

Upstream 100 bases:

>100_bases
CGGCCAGCTGATCCTGCACAAGACATTCCCTGTCCTGAACAAGGATTACGAGAAACGAGAATCAAGGAGCGCACGGGCCG
CCAGATAGGGGAGCCGAGCG

Downstream 100 bases:

>100_bases
GCATGGATCTCGAGTTCGGTTGCGTTAGTTGCGTTGCGTTTCCCTCTCACCATCCACATGGAGACTGACATGTCTGACTT
TACTGATGCTGATATCGTCG

Product: replication protein

Products: NA

Alternate protein names: Replication protein [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MLSEPSIDPLQHEWMGAASSQVLLGLDARPDFICEQIDPPVDLRDRDNLPDVGKRLGKVGRRDFQRLQQEKLAIAESLKE
AGRDEDAGLLRNCCTNLIIDKYEKGRRVRGPNECKHPLCPTAQLARQRRTYRRVAQALERYLRRKEGMVGIMLTTTMESC
PSHELRYRTGQLIAACSRLMTFAPVKRAVAAYVRVIEFTRNAVTQLWHPHAHWLLLVPKSYFAKGSKTFIDHDRWSKLFR
KALRCAYTPIVDVRVAKGVSSPLSEAGLKPLREIAKYTTKPGTLVYWDNGMPYAVGRQRPELYRKAKGAPLEPMFNVPVM
ALADAVKGRRLVALSRNLETDEELDFTDDPRGDELDQTTEDLGAFICTEFYEWRGRGRHGDYFLVGRSFDEPGQHKRKEG
FAMGP

Sequences:

>Translated_405_residues
MLSEPSIDPLQHEWMGAASSQVLLGLDARPDFICEQIDPPVDLRDRDNLPDVGKRLGKVGRRDFQRLQQEKLAIAESLKE
AGRDEDAGLLRNCCTNLIIDKYEKGRRVRGPNECKHPLCPTAQLARQRRTYRRVAQALERYLRRKEGMVGIMLTTTMESC
PSHELRYRTGQLIAACSRLMTFAPVKRAVAAYVRVIEFTRNAVTQLWHPHAHWLLLVPKSYFAKGSKTFIDHDRWSKLFR
KALRCAYTPIVDVRVAKGVSSPLSEAGLKPLREIAKYTTKPGTLVYWDNGMPYAVGRQRPELYRKAKGAPLEPMFNVPVM
ALADAVKGRRLVALSRNLETDEELDFTDDPRGDELDQTTEDLGAFICTEFYEWRGRGRHGDYFLVGRSFDEPGQHKRKEG
FAMGP
>Mature_405_residues
MLSEPSIDPLQHEWMGAASSQVLLGLDARPDFICEQIDPPVDLRDRDNLPDVGKRLGKVGRRDFQRLQQEKLAIAESLKE
AGRDEDAGLLRNCCTNLIIDKYEKGRRVRGPNECKHPLCPTAQLARQRRTYRRVAQALERYLRRKEGMVGIMLTTTMESC
PSHELRYRTGQLIAACSRLMTFAPVKRAVAAYVRVIEFTRNAVTQLWHPHAHWLLLVPKSYFAKGSKTFIDHDRWSKLFR
KALRCAYTPIVDVRVAKGVSSPLSEAGLKPLREIAKYTTKPGTLVYWDNGMPYAVGRQRPELYRKAKGAPLEPMFNVPVM
ALADAVKGRRLVALSRNLETDEELDFTDDPRGDELDQTTEDLGAFICTEFYEWRGRGRHGDYFLVGRSFDEPGQHKRKEG
FAMGP

Specific function: Produces a single-strand nick in a specific site of the plasmid, and this nick results in single-strand replication by rolling circle mechanism [H]

COG id: COG5655

COG function: function code L; Plasmid rolling circle replication initiator protein and truncated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-positive plasmids replication protein type 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000989 [H]

Pfam domain/function: PF01446 Rep_1 [H]

EC number: NA

Molecular weight: Translated: 46159; Mature: 46159

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9887308 [H]