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The map label for this gene is glcA [H]

Identifier: 146342937

GI number: 146342937

Start: 6345278

End: 6346942

Strand: Reverse

Name: glcA [H]

Synonym: BRADO6118

Alternate gene names: 146342937

Gene position: 6346942-6345278 (Counterclockwise)

Preceding gene: 146342939

Following gene: 146342936

Centisome position: 85.12

GC content: 66.01

Gene sequence:

>1665_bases
ATGTGGAATCAAGTCTACGACCCGTTGGGGAGCCCGGCGCTGTCGACGCTGGCCGCCGCCGTCCCGGTGGTGACGCTGCT
GGTGTTGATCGCCAGCGGCAAGGTGAAGGCGCATATCGCCGCGATCATCGCGGTGGTGCTGACCAACATCATCACGATCT
TCGTCTTCACCATGCCGGCCAACATGTCGCTGCGCGCAACCGTGCTCGGCGTCGTCACCGGCTTCTTCCCGATCGGCTGG
ATCGTGCTGAACGTCATCTTCCTCTACCAGGTGACGGTCTCGACCGGCCGCTTCGAGCTGCTCAAGCGCGCGATCGGCGG
TGTGACGGAAGATCGCCGGCTGCAGCTGCTGCTGGTCGCGTTCTCGTTCGGCGCGTTCTTCGAGGGCGCGTCCGGCTTCG
GCACGCCGGTGGCGATCACCGGTGCCGTGCTGATCGGCCTCGGCTTCTCGCCGCTCGCCGCCTCCGGCCTGTCGTTGATC
GCCAACACCGCGCCGGTCGCCTATGGCGCGCTGGGCACGCCGATCCAGGGCCTCGCCTCGGTCACCGGCCTCGATCCCTA
CATCCTCGGCGCGATGGTCGGCCGGCAGCTGCCGGTCTTCTCGCTGATCGTGCCGTTCTGGGTGATCTGGGCGTTCGCCG
GCTGGAAGGGCATGAAGGACATCTGGCCCGCGATCCTGGTCACCGGCGTGTCCTTCGCCGTCCCGCAGTTCCTGATCTCG
AACTACATCAATCCGTGGATCGTCGACATCGGCGCCTCGCTGATCTCGATGGGCGCGCTGATCCTGTTCCTGAGGGTCTG
GCAGCCGAAACAGCTGTGGCTGTCGCCGGCGCTGCGCGGCCGCGACGAATCGGCCGCCACCATGGCGGCCGCCAAGCCGC
TCGACCGTACGCCGCTGACGCAGGCCGAGCTGTGGGGCGCGCTGCTGCCCTGGATCATCGTCTGCATCATCATGCTGATC
TGGGGTAACGGCGCCTTCAAGAGCTGGGCGAACTCGATCTTCACCTGGAGCTATCCGGTGCCGGAGCTCGACAAGCTGAT
CAACAAGATGCCGCCGGTGGCCGCGAAGCCGACGCCCGAGGGCGCGGTGTTCTCGTTCACCTATCTGACCTTCACCGGCA
CCGGCATGCTGATCGCGGCGATTATCTCCGGCTTCCTGATGGGCGTCGGACCGGGCAAGCTGATCGCCGAATATGGCCGC
ACCATCCGTCTCTGCGCGATCTCGCTGATCACGATCTCGGCGATGCTCGCGATCGGCACGCTCACGCGCCTGTCCGGCGT
CGACGCGACGCTGGGCCTCGCCTTCGCCGCGACCGGCGTGCTCTATCCCTTCTTCGGCACTTTGCTCGGCTGGCTGGGCG
TGGCGCTGACGGGATCGGACACCGCCTCCAACGTGCTGTTCGGCAATCTGCAGAAGATCACCTCCGAGCAGCTCGGCCTG
TCGCCGATCCTGATGGGGGCGGCCAACTCCTCGGGCGGCGTGATGGGCAAGATGATCGACGCGCAATCGATCGTCGTCGC
CTCGACCGCCACCAATTGGTACGGCCATGAGAGCTCGATCCTGCGCTACGTCTTCCTGCACTCGATCGTGCTGGCCTGTC
TGGTCGGCCTGTTCGTGACCCTGCAGGCCTATGTCTACCCGTTCACCGCGATGGTGCTGAAGTAG

Upstream 100 bases:

>100_bases
CGGGTTTCGTGACCGTCCCCGTACGGTCGTGGCACCTGCAGACAGACCTGATCCGCCCCGCCGGACCGGGTCCAACAACA
AGAATTGGGGGGAAGTCACG

Downstream 100 bases:

>100_bases
CACCACGCCTGCGACAAAAACCCCGCGCCGCAACCGCGGCGCGGGGTTTGCTATGTTTGGACCACATTTCTGCGGGAGCC
GTGCTGGCGGCCACTCAGCC

Product: LctP family glycolate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MWNQVYDPLGSPALSTLAAAVPVVTLLVLIASGKVKAHIAAIIAVVLTNIITIFVFTMPANMSLRATVLGVVTGFFPIGW
IVLNVIFLYQVTVSTGRFELLKRAIGGVTEDRRLQLLLVAFSFGAFFEGASGFGTPVAITGAVLIGLGFSPLAASGLSLI
ANTAPVAYGALGTPIQGLASVTGLDPYILGAMVGRQLPVFSLIVPFWVIWAFAGWKGMKDIWPAILVTGVSFAVPQFLIS
NYINPWIVDIGASLISMGALILFLRVWQPKQLWLSPALRGRDESAATMAAAKPLDRTPLTQAELWGALLPWIIVCIIMLI
WGNGAFKSWANSIFTWSYPVPELDKLINKMPPVAAKPTPEGAVFSFTYLTFTGTGMLIAAIISGFLMGVGPGKLIAEYGR
TIRLCAISLITISAMLAIGTLTRLSGVDATLGLAFAATGVLYPFFGTLLGWLGVALTGSDTASNVLFGNLQKITSEQLGL
SPILMGAANSSGGVMGKMIDAQSIVVASTATNWYGHESSILRYVFLHSIVLACLVGLFVTLQAYVYPFTAMVLK

Sequences:

>Translated_554_residues
MWNQVYDPLGSPALSTLAAAVPVVTLLVLIASGKVKAHIAAIIAVVLTNIITIFVFTMPANMSLRATVLGVVTGFFPIGW
IVLNVIFLYQVTVSTGRFELLKRAIGGVTEDRRLQLLLVAFSFGAFFEGASGFGTPVAITGAVLIGLGFSPLAASGLSLI
ANTAPVAYGALGTPIQGLASVTGLDPYILGAMVGRQLPVFSLIVPFWVIWAFAGWKGMKDIWPAILVTGVSFAVPQFLIS
NYINPWIVDIGASLISMGALILFLRVWQPKQLWLSPALRGRDESAATMAAAKPLDRTPLTQAELWGALLPWIIVCIIMLI
WGNGAFKSWANSIFTWSYPVPELDKLINKMPPVAAKPTPEGAVFSFTYLTFTGTGMLIAAIISGFLMGVGPGKLIAEYGR
TIRLCAISLITISAMLAIGTLTRLSGVDATLGLAFAATGVLYPFFGTLLGWLGVALTGSDTASNVLFGNLQKITSEQLGL
SPILMGAANSSGGVMGKMIDAQSIVVASTATNWYGHESSILRYVFLHSIVLACLVGLFVTLQAYVYPFTAMVLK
>Mature_554_residues
MWNQVYDPLGSPALSTLAAAVPVVTLLVLIASGKVKAHIAAIIAVVLTNIITIFVFTMPANMSLRATVLGVVTGFFPIGW
IVLNVIFLYQVTVSTGRFELLKRAIGGVTEDRRLQLLLVAFSFGAFFEGASGFGTPVAITGAVLIGLGFSPLAASGLSLI
ANTAPVAYGALGTPIQGLASVTGLDPYILGAMVGRQLPVFSLIVPFWVIWAFAGWKGMKDIWPAILVTGVSFAVPQFLIS
NYINPWIVDIGASLISMGALILFLRVWQPKQLWLSPALRGRDESAATMAAAKPLDRTPLTQAELWGALLPWIIVCIIMLI
WGNGAFKSWANSIFTWSYPVPELDKLINKMPPVAAKPTPEGAVFSFTYLTFTGTGMLIAAIISGFLMGVGPGKLIAEYGR
TIRLCAISLITISAMLAIGTLTRLSGVDATLGLAFAATGVLYPFFGTLLGWLGVALTGSDTASNVLFGNLQKITSEQLGL
SPILMGAANSSGGVMGKMIDAQSIVVASTATNWYGHESSILRYVFLHSIVLACLVGLFVTLQAYVYPFTAMVLK

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=562, Percent_Identity=45.5516014234875, Blast_Score=460, Evalue=1e-130,
Organism=Escherichia coli, GI1790031, Length=550, Percent_Identity=42.3636363636364, Blast_Score=419, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 58747; Mature: 58747

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNQVYDPLGSPALSTLAAAVPVVTLLVLIASGKVKAHIAAIIAVVLTNIITIFVFTMPA
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NMSLRATVLGVVTGFFPIGWIVLNVIFLYQVTVSTGRFELLKRAIGGVTEDRRLQLLLVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHH
FSFGAFFEGASGFGTPVAITGAVLIGLGFSPLAASGLSLIANTAPVAYGALGTPIQGLAS
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHH
VTGLDPYILGAMVGRQLPVFSLIVPFWVIWAFAGWKGMKDIWPAILVTGVSFAVPQFLIS
HCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
NYINPWIVDIGASLISMGALILFLRVWQPKQLWLSPALRGRDESAATMAAAKPLDRTPLT
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHCCCCCHHHHHHHCCCCCCCCCH
QAELWGALLPWIIVCIIMLIWGNGAFKSWANSIFTWSYPVPELDKLINKMPPVAAKPTPE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCCCCCC
GAVFSFTYLTFTGTGMLIAAIISGFLMGVGPGKLIAEYGRTIRLCAISLITISAMLAIGT
CCEEEEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LTRLSGVDATLGLAFAATGVLYPFFGTLLGWLGVALTGSDTASNVLFGNLQKITSEQLGL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCC
SPILMGAANSSGGVMGKMIDAQSIVVASTATNWYGHESSILRYVFLHSIVLACLVGLFVT
CCEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LQAYVYPFTAMVLK
HHHHHHHHHHHHCC
>Mature Secondary Structure
MWNQVYDPLGSPALSTLAAAVPVVTLLVLIASGKVKAHIAAIIAVVLTNIITIFVFTMPA
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NMSLRATVLGVVTGFFPIGWIVLNVIFLYQVTVSTGRFELLKRAIGGVTEDRRLQLLLVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHH
FSFGAFFEGASGFGTPVAITGAVLIGLGFSPLAASGLSLIANTAPVAYGALGTPIQGLAS
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHH
VTGLDPYILGAMVGRQLPVFSLIVPFWVIWAFAGWKGMKDIWPAILVTGVSFAVPQFLIS
HCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
NYINPWIVDIGASLISMGALILFLRVWQPKQLWLSPALRGRDESAATMAAAKPLDRTPLT
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHCCCCCHHHHHHHCCCCCCCCCH
QAELWGALLPWIIVCIIMLIWGNGAFKSWANSIFTWSYPVPELDKLINKMPPVAAKPTPE
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCCCCCC
GAVFSFTYLTFTGTGMLIAAIISGFLMGVGPGKLIAEYGRTIRLCAISLITISAMLAIGT
CCEEEEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LTRLSGVDATLGLAFAATGVLYPFFGTLLGWLGVALTGSDTASNVLFGNLQKITSEQLGL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCC
SPILMGAANSSGGVMGKMIDAQSIVVASTATNWYGHESSILRYVFLHSIVLACLVGLFVT
CCEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LQAYVYPFTAMVLK
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]