Click here to switch to the map view.

The map label for this gene is pmbA [H]

Identifier: 146342919

GI number: 146342919

Start: 6327405

End: 6328799

Strand: Reverse

Name: pmbA [H]

Synonym: BRADO6100

Alternate gene names: 146342919

Gene position: 6328799-6327405 (Counterclockwise)

Preceding gene: 146342921

Following gene: 146342918

Centisome position: 84.88

GC content: 68.46

Gene sequence:

>1395_bases
GTGACCTCCTCCCCAACAGCTGCTGCATCATCGACCTCCGCCCATTCAGATCTGCTCGATCAGTCCGCCCTGAGAACGCT
TGCCGAGCGGCTGGTCGAGGCCGCGAAGAAGGCCGGCGCCGATGCCGCCGATGCGATCGCCGTGCGCGGCATCTCGCAGG
GCGTGGAGGTGCGTGACGGGCGCGTCGAGGAATCGGAACGCTCCGAAGGCGACGATGTCGGCTTGCGCGTGCTGGTCGGC
AAGCGGCAGGCGGTGGTGTCGACCAACGATGTCAGCGGCGATGGCGTGGCGCGGCTCGCCGAGCGCGCCGTGGCCATGGC
GCGCGTGGCGCCCGACGACAAATATGTCGGCCTCGCCGATCCCGAGCTTCTGGCGCGCGACATTCCCGAGCTCGATCTGC
TCGACGCGAACATCCCTGGGACGACCGAGCTGGAGCGGCGCGCCCAGGAGGCGGAGGCCGCCGCCCTCGCCGTGAAGGGC
GTCACCAAGTCCGGCGGCGCGTCGGCCTCGGCCGGTATCGGCGGCATGGTGCTCGTGACGAGCAGCGGCTTTCACGGCGC
CTATCTGCGCTCGAGCCAGAGCATCTCGACGACCGCGATTTCCGGCGAAGGCACCGGCATGGAGCGCGACTACGACTACA
CGGCCGCGCCGCATGGCGCCGACCTGCTCGCGCCGGCCGCCGTCGGACGCAGCGCCGGCGAGCGCGCGGTGGCGCGCGCC
AACCCCCGCAAGGTCGCAACCTGCCAGGTCCCGGTCGTGTTCGATCCCCGTGTGTCGGGCTCGCTGGTCGGCCATCTCGT
CGGCGCTATCAACGGCGCCTCGATCGCGCGCAAGACCAGCTTCCTGAAGGACAAGCTCGGCGCGCAATTGTTCGCCAAGG
GCATCCGCATCATCGACGATCCGTTGCGTCCGCGGGGCCTGCGCTCGCAGCCGTTCGATGCCGAGGGCGTCAAGGTGAAG
AAGACGGCGCTGGTCGATGACGGCGTGCTCACCACCTGGCTGTTGGACTCCGCGACGGCACGCGAGCTTGGCCTCGTCAC
CACCGGCCACGCCCATCGCGGCGTCTCGTCCTCGCCGTCGCCGGGCCCGTTCAATCTGCATCTCGAGGCCGGCGTCGTGA
CGCCGAAGGAGCTGATCTCCGACATCAAGCAGGGCTTCTATGTCACCGACCTGATCGGTTCGGGCGTCAACGGCGTCACC
GGTGATTACAGCCGCGGCGCCTCGGGCTTCTGGATTGAGAATGGCGAGATCGGCTACGCCGTGAGCGAGGTGACGATCGC
AGGCCATCTGTTCGAGATCTTCAAGTCGATGACCCCCGCCAGCAATCTGGAATTCCGCTATGGCGTCAATGCGCCGACGG
TGCGCATCGAAGGGTTGACGCTTGGCGGACGGTGA

Upstream 100 bases:

>100_bases
TGTGGTGCTTGACCTGCTGCTTGAAGTCGGCGTGATCAGGCCACATTTCGTCTGGATTGAGCCGTCTTCGCCGCCACCTG
ACATCGATCGGATCGTGTTC

Downstream 100 bases:

>100_bases
GATCGAGACATCCAGTGAGACCGGCTTGGCGGCTGATGCCGGGCTGCTGAAGGAGGCGGTGCGCGAGGCCGGCGAACTGG
CGCACGCGATGTTCGGCACC

Product: putative Zn-dependent protease, pmbA-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTSSPTAAASSTSAHSDLLDQSALRTLAERLVEAAKKAGADAADAIAVRGISQGVEVRDGRVEESERSEGDDVGLRVLVG
KRQAVVSTNDVSGDGVARLAERAVAMARVAPDDKYVGLADPELLARDIPELDLLDANIPGTTELERRAQEAEAAALAVKG
VTKSGGASASAGIGGMVLVTSSGFHGAYLRSSQSISTTAISGEGTGMERDYDYTAAPHGADLLAPAAVGRSAGERAVARA
NPRKVATCQVPVVFDPRVSGSLVGHLVGAINGASIARKTSFLKDKLGAQLFAKGIRIIDDPLRPRGLRSQPFDAEGVKVK
KTALVDDGVLTTWLLDSATARELGLVTTGHAHRGVSSSPSPGPFNLHLEAGVVTPKELISDIKQGFYVTDLIGSGVNGVT
GDYSRGASGFWIENGEIGYAVSEVTIAGHLFEIFKSMTPASNLEFRYGVNAPTVRIEGLTLGGR

Sequences:

>Translated_464_residues
MTSSPTAAASSTSAHSDLLDQSALRTLAERLVEAAKKAGADAADAIAVRGISQGVEVRDGRVEESERSEGDDVGLRVLVG
KRQAVVSTNDVSGDGVARLAERAVAMARVAPDDKYVGLADPELLARDIPELDLLDANIPGTTELERRAQEAEAAALAVKG
VTKSGGASASAGIGGMVLVTSSGFHGAYLRSSQSISTTAISGEGTGMERDYDYTAAPHGADLLAPAAVGRSAGERAVARA
NPRKVATCQVPVVFDPRVSGSLVGHLVGAINGASIARKTSFLKDKLGAQLFAKGIRIIDDPLRPRGLRSQPFDAEGVKVK
KTALVDDGVLTTWLLDSATARELGLVTTGHAHRGVSSSPSPGPFNLHLEAGVVTPKELISDIKQGFYVTDLIGSGVNGVT
GDYSRGASGFWIENGEIGYAVSEVTIAGHLFEIFKSMTPASNLEFRYGVNAPTVRIEGLTLGGR
>Mature_463_residues
TSSPTAAASSTSAHSDLLDQSALRTLAERLVEAAKKAGADAADAIAVRGISQGVEVRDGRVEESERSEGDDVGLRVLVGK
RQAVVSTNDVSGDGVARLAERAVAMARVAPDDKYVGLADPELLARDIPELDLLDANIPGTTELERRAQEAEAAALAVKGV
TKSGGASASAGIGGMVLVTSSGFHGAYLRSSQSISTTAISGEGTGMERDYDYTAAPHGADLLAPAAVGRSAGERAVARAN
PRKVATCQVPVVFDPRVSGSLVGHLVGAINGASIARKTSFLKDKLGAQLFAKGIRIIDDPLRPRGLRSQPFDAEGVKVKK
TALVDDGVLTTWLLDSATARELGLVTTGHAHRGVSSSPSPGPFNLHLEAGVVTPKELISDIKQGFYVTDLIGSGVNGVTG
DYSRGASGFWIENGEIGYAVSEVTIAGHLFEIFKSMTPASNLEFRYGVNAPTVRIEGLTLGGR

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=449, Percent_Identity=36.9710467706013, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1789640, Length=232, Percent_Identity=25.4310344827586, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48108; Mature: 47976

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSPTAAASSTSAHSDLLDQSALRTLAERLVEAAKKAGADAADAIAVRGISQGVEVRDG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEECCC
RVEESERSEGDDVGLRVLVGKRQAVVSTNDVSGDGVARLAERAVAMARVAPDDKYVGLAD
CCCCHHCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCC
PELLARDIPELDLLDANIPGTTELERRAQEAEAAALAVKGVTKSGGASASAGIGGMVLVT
HHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEE
SSGFHGAYLRSSQSISTTAISGEGTGMERDYDYTAAPHGADLLAPAAVGRSAGERAVARA
ECCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHCC
NPRKVATCQVPVVFDPRVSGSLVGHLVGAINGASIARKTSFLKDKLGAQLFAKGIRIIDD
CCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEECC
PLRPRGLRSQPFDAEGVKVKKTALVDDGVLTTWLLDSATARELGLVTTGHAHRGVSSSPS
CCCCCCCCCCCCCCCCEEEEEEEEECCCCEEHHHHCCCCHHHHCEEECCCCCCCCCCCCC
PGPFNLHLEAGVVTPKELISDIKQGFYVTDLIGSGVNGVTGDYSRGASGFWIENGEIGYA
CCCEEEEEECCCCCHHHHHHHHHCCEEEEHHHCCCCCCCCCCCCCCCCCEEEECCCCCEE
VSEVTIAGHLFEIFKSMTPASNLEFRYGVNAPTVRIEGLTLGGR
EHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEECCC
>Mature Secondary Structure 
TSSPTAAASSTSAHSDLLDQSALRTLAERLVEAAKKAGADAADAIAVRGISQGVEVRDG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEECCC
RVEESERSEGDDVGLRVLVGKRQAVVSTNDVSGDGVARLAERAVAMARVAPDDKYVGLAD
CCCCHHCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCC
PELLARDIPELDLLDANIPGTTELERRAQEAEAAALAVKGVTKSGGASASAGIGGMVLVT
HHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEE
SSGFHGAYLRSSQSISTTAISGEGTGMERDYDYTAAPHGADLLAPAAVGRSAGERAVARA
ECCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHCC
NPRKVATCQVPVVFDPRVSGSLVGHLVGAINGASIARKTSFLKDKLGAQLFAKGIRIIDD
CCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEECC
PLRPRGLRSQPFDAEGVKVKKTALVDDGVLTTWLLDSATARELGLVTTGHAHRGVSSSPS
CCCCCCCCCCCCCCCCEEEEEEEEECCCCEEHHHHCCCCHHHHCEEECCCCCCCCCCCCC
PGPFNLHLEAGVVTPKELISDIKQGFYVTDLIGSGVNGVTGDYSRGASGFWIENGEIGYA
CCCEEEEEECCCCCHHHHHHHHHCCEEEEHHHCCCCCCCCCCCCCCCCCEEEECCCCCEE
VSEVTIAGHLFEIFKSMTPASNLEFRYGVNAPTVRIEGLTLGGR
EHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]