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The map label for this gene is kdtA [H]

Identifier: 146342915

GI number: 146342915

Start: 6324185

End: 6325492

Strand: Reverse

Name: kdtA [H]

Synonym: BRADO6096

Alternate gene names: 146342915

Gene position: 6325492-6324185 (Counterclockwise)

Preceding gene: 146342916

Following gene: 146342914

Centisome position: 84.83

GC content: 69.34

Gene sequence:

>1308_bases
GTGGCTAGCCCGCTGCCGATGGCCCTGCGTGTCTACCGCGGGTTGTCCTCGGCGGCGGTGCCGCTTGCTCCGGCGCTGAT
CAGGCAGCGGCTCAAGCGCGGCAAGGAGGACCCGGAGCGCAGCGACGAGCGGCGCGGCCTCAGCCACGACACCCGCCCAC
AGGGACCTCTGGTCTGGATTCACGGTGCTAGCGTCGGCGAAGTCCTGGCGGCCGCGGCGCTGATCGAGCGGCTGCGAGAG
CTGAACATCCGTATCCTGATCACCTCGGGCACCGTGACGTCCGCTGCGATCGTCGCCAAGCGTTTCCCGCCCGACGTCAT
CCACCAATACGTCCCCTACGACACGCCGCGTTTCGTCGAGCGCTTCATCGACCATTGGCGGCCATCGCTCGGCCTGTTCA
TCGAATCCGATCTGTGGCCGAACCTGATCCTGGCCGGCGCGTCGCGGCGGGTGCCGATGGTCGTGATCAACGGCCGCATG
TCGCAGCGCTCGTTCCCGCGCTGGCGCCGCATGGCTGGTACGATCTCCGCACTGCTCGGACGCTTCGATCTCTGCCTGAC
GCAGTCGGAAGCCGATGCCGAGCGGTTCTCCGCACTCGGGGCGCGCAACGTCATCACGACAGGCAATCTCAAGCTCGACG
TGCCGGCGCCGCCGGCCGATGCGGCGAAGCTCGACCGGCTCACCGCGATGACGCGCGGCCGTCCGGTAGTGGTCGCCGCC
TCGACGCATCCCGGCGAGGAGGAGATCCTGATTGGCGCCCACAAGGCGTTGGCGGCGACTCACCCGTCTCTGCTCACGGT
GATTGTGCCGCGGCATCCGCATCGCGGTCCGGCGGTTGCCGAGCTCATCGCCGGCGCGGGACTGCACGGCGCGCTACGCT
CGCGTGAGGAGCAGCCGCTCGCCGGCACCGACATCTACGTCGCTGACACGATGGGCGAGCTCGGCCTGTTCTACCGGCTG
GCGCCGATCGTGTTCATGGGTGGCTCGCTGGTCGAGCATGGCGGCCAGAATCCGATCGAGGCGGTGAAGCTCGGCGCCGC
GGTCGTGCATGGGCCGCATGTCTTCAACTTCACCGATGTCTACGAAGCGCTCGACAAGGCGGCCGGCGCGCGTCTCGCCC
ATGACCGGGAGGCGCTGGTGCGGCAACTCCGGCAACTGCTTGCCGCGCCCGAGGCCTGCGCCACGATAGTGGCGGCGGGC
TCGCGCGTCGTCGACCAGCTCGGCGGCGCGCTGGAGCGGACGATGGCCGCGCTCGAGCCTTATCTTTTGCAGGTCAGGCT
CGAGATGGGGGCCGCCGGCGATGCGTGA

Upstream 100 bases:

>100_bases
ATGCGCCGCGCCTATGAGATCGTAGGCCGCCCCGAGGCCTGCCCGCCGCCGGTGAATTCGGGCGCGGACTCTTCAAGAGC
GGAATCTTCGGGAGCGTCCA

Downstream 100 bases:

>100_bases
GCCGGCCTTCTGGCATCGGCCCCCCTCCTGGCAATCGCATCTGTTGTCTCCGCTGTCGATGCTGTACGGCGCCGTCGCTG
CGCGCCGCATGGCGCAGCCT

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MASPLPMALRVYRGLSSAAVPLAPALIRQRLKRGKEDPERSDERRGLSHDTRPQGPLVWIHGASVGEVLAAAALIERLRE
LNIRILITSGTVTSAAIVAKRFPPDVIHQYVPYDTPRFVERFIDHWRPSLGLFIESDLWPNLILAGASRRVPMVVINGRM
SQRSFPRWRRMAGTISALLGRFDLCLTQSEADAERFSALGARNVITTGNLKLDVPAPPADAAKLDRLTAMTRGRPVVVAA
STHPGEEEILIGAHKALAATHPSLLTVIVPRHPHRGPAVAELIAGAGLHGALRSREEQPLAGTDIYVADTMGELGLFYRL
APIVFMGGSLVEHGGQNPIEAVKLGAAVVHGPHVFNFTDVYEALDKAAGARLAHDREALVRQLRQLLAAPEACATIVAAG
SRVVDQLGGALERTMAALEPYLLQVRLEMGAAGDA

Sequences:

>Translated_435_residues
MASPLPMALRVYRGLSSAAVPLAPALIRQRLKRGKEDPERSDERRGLSHDTRPQGPLVWIHGASVGEVLAAAALIERLRE
LNIRILITSGTVTSAAIVAKRFPPDVIHQYVPYDTPRFVERFIDHWRPSLGLFIESDLWPNLILAGASRRVPMVVINGRM
SQRSFPRWRRMAGTISALLGRFDLCLTQSEADAERFSALGARNVITTGNLKLDVPAPPADAAKLDRLTAMTRGRPVVVAA
STHPGEEEILIGAHKALAATHPSLLTVIVPRHPHRGPAVAELIAGAGLHGALRSREEQPLAGTDIYVADTMGELGLFYRL
APIVFMGGSLVEHGGQNPIEAVKLGAAVVHGPHVFNFTDVYEALDKAAGARLAHDREALVRQLRQLLAAPEACATIVAAG
SRVVDQLGGALERTMAALEPYLLQVRLEMGAAGDA
>Mature_434_residues
ASPLPMALRVYRGLSSAAVPLAPALIRQRLKRGKEDPERSDERRGLSHDTRPQGPLVWIHGASVGEVLAAAALIERLREL
NIRILITSGTVTSAAIVAKRFPPDVIHQYVPYDTPRFVERFIDHWRPSLGLFIESDLWPNLILAGASRRVPMVVINGRMS
QRSFPRWRRMAGTISALLGRFDLCLTQSEADAERFSALGARNVITTGNLKLDVPAPPADAAKLDRLTAMTRGRPVVVAAS
THPGEEEILIGAHKALAATHPSLLTVIVPRHPHRGPAVAELIAGAGLHGALRSREEQPLAGTDIYVADTMGELGLFYRLA
PIVFMGGSLVEHGGQNPIEAVKLGAAVVHGPHVFNFTDVYEALDKAAGARLAHDREALVRQLRQLLAAPEACATIVAAGS
RVVDQLGGALERTMAALEPYLLQVRLEMGAAGDA

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=418, Percent_Identity=40.9090909090909, Blast_Score=271, Evalue=8e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 46883; Mature: 46752

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPLPMALRVYRGLSSAAVPLAPALIRQRLKRGKEDPERSDERRGLSHDTRPQGPLVWI
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCEEEE
HGASVGEVLAAAALIERLRELNIRILITSGTVTSAAIVAKRFPPDVIHQYVPYDTPRFVE
ECCCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCHHHHH
RFIDHWRPSLGLFIESDLWPNLILAGASRRVPMVVINGRMSQRSFPRWRRMAGTISALLG
HHHHHCCCCCCEEEECCCCCCCEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
RFDLCLTQSEADAERFSALGARNVITTGNLKLDVPAPPADAAKLDRLTAMTRGRPVVVAA
HHHHHEECCHHHHHHHHHHCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHCCCEEEEEE
STHPGEEEILIGAHKALAATHPSLLTVIVPRHPHRGPAVAELIAGAGLHGALRSREEQPL
CCCCCCCCEEEECHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHHHCCCCCCC
AGTDIYVADTMGELGLFYRLAPIVFMGGSLVEHGGQNPIEAVKLGAAVVHGPHVFNFTDV
CCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHH
YEALDKAAGARLAHDREALVRQLRQLLAAPEACATIVAAGSRVVDQLGGALERTMAALEP
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLLQVRLEMGAAGDA
HHHHEEECCCCCCCC
>Mature Secondary Structure 
ASPLPMALRVYRGLSSAAVPLAPALIRQRLKRGKEDPERSDERRGLSHDTRPQGPLVWI
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCEEEE
HGASVGEVLAAAALIERLRELNIRILITSGTVTSAAIVAKRFPPDVIHQYVPYDTPRFVE
ECCCHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCHHHHH
RFIDHWRPSLGLFIESDLWPNLILAGASRRVPMVVINGRMSQRSFPRWRRMAGTISALLG
HHHHHCCCCCCEEEECCCCCCCEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
RFDLCLTQSEADAERFSALGARNVITTGNLKLDVPAPPADAAKLDRLTAMTRGRPVVVAA
HHHHHEECCHHHHHHHHHHCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHCCCEEEEEE
STHPGEEEILIGAHKALAATHPSLLTVIVPRHPHRGPAVAELIAGAGLHGALRSREEQPL
CCCCCCCCEEEECHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHHHCCCCCCC
AGTDIYVADTMGELGLFYRLAPIVFMGGSLVEHGGQNPIEAVKLGAAVVHGPHVFNFTDV
CCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHH
YEALDKAAGARLAHDREALVRQLRQLLAAPEACATIVAAGSRVVDQLGGALERTMAALEP
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLLQVRLEMGAAGDA
HHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]