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The map label for this gene is ribF [H]

Identifier: 146342886

GI number: 146342886

Start: 6290182

End: 6291162

Strand: Direct

Name: ribF [H]

Synonym: BRADO6066

Alternate gene names: 146342886

Gene position: 6290182-6291162 (Clockwise)

Preceding gene: 146342884

Following gene: 146342887

Centisome position: 84.36

GC content: 68.91

Gene sequence:

>981_bases
ATGCCAGCTTTCCAGATCATCCGCGATGCCACCCCGCCACAGCACATCCTCAAGGGAGCGGTGGTGGCGATGGGAAATTT
CGACGGTGTCCATCTTGGTCACCGGGCCGTGATCGAGGCCGCCATCGAGATGGGCAGGGCCAAGGGCCGCCCGGCCCTGG
CGCTGACCTTCGAGCCGCATCCGCGGCGCTTCTTCAGCCCCAACACCCCGCAATTCCGGCTGACCGACGAGGCGGCCAAG
CTGCGGCTGCTGGCCGGAGCCGGGCTGGCCGGCGCCGTGGTCATGACCTTCGACAAGGGCCGCGCCCAGACCTCGGCGCA
GGATTTCATTCACCACGATCTCATCGGCCGGCTGGGCGTGTCCGGGATCGCCGTCGGCTACGACTTTCATTTTGGCAAGG
GCCGGGTCGGCTCGCCCAGCCTGCTCGCCAGCGAGGCGCCGAAGCTCGGCATCACGGTCGAGGTCCAGCCGCATGTCGAC
ATCGACGAACGGCCGGTGTCCTCCACCGCGATCCGCGAGGCGCTGGCCGAAGGCCAAGTGGCCGAGGCCACCGCCATGCT
CGGCGGCCCCTGGTTCGTGACGGCGGAGGTCATCCACGGCGAGAAGCGCGGCCGCGACCTCGGCTATCCCACCGCCAACA
TGCGGCTCGACCGCGATTGCGGCCTGCGCCACGGCATCTATGCCGTCCGGGTCGGCCGCGGCCGCGGTGCGAACCGGGTC
CTGATCGATGGCGTCGCCAATTTTGGCCGCCGCCCGACCTTCGACAACGGCGCGCCGCTGCTGGAGACCTTCCTGTTCGA
CTTCAAGGACAGCCTCTATGGCGAGGTGCTGGACGTCGCCTTCATCGGCTTCATCCGCGAGGAGGCCAGATTCACCTCCA
TCGAGGCCCTGATCCGGCAGATGGACGACGACAGCGCGAGGGCACGCGCCGTGCTGGCGGCAAACGCCGGCCTCTTCCCC
CGGCTCGGCGAGATCGGCTGA

Upstream 100 bases:

>100_bases
TCGGAACCCCTGCGGCTTGACTTCGGCAGGCCGGCCGCCCACGGTCGAGGCCAGCTTCGCCGCACCGCGGCTTGATGAAT
TCGCGAGATATTTCCGTCAG

Downstream 100 bases:

>100_bases
TTCTCCACCGCGTCCGTCATACCGGGGCAGCCCGCGAGGGCTGAACCCGGAATCTCGAGATTCCGGGCTCGATGCTTCGC
ATCGCCCCGGAATGACGCCG

Product: riboflavin biosynthesis protein ribF/bifunctional flavokinase/FAD synthetase

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MPAFQIIRDATPPQHILKGAVVAMGNFDGVHLGHRAVIEAAIEMGRAKGRPALALTFEPHPRRFFSPNTPQFRLTDEAAK
LRLLAGAGLAGAVVMTFDKGRAQTSAQDFIHHDLIGRLGVSGIAVGYDFHFGKGRVGSPSLLASEAPKLGITVEVQPHVD
IDERPVSSTAIREALAEGQVAEATAMLGGPWFVTAEVIHGEKRGRDLGYPTANMRLDRDCGLRHGIYAVRVGRGRGANRV
LIDGVANFGRRPTFDNGAPLLETFLFDFKDSLYGEVLDVAFIGFIREEARFTSIEALIRQMDDDSARARAVLAANAGLFP
RLGEIG

Sequences:

>Translated_326_residues
MPAFQIIRDATPPQHILKGAVVAMGNFDGVHLGHRAVIEAAIEMGRAKGRPALALTFEPHPRRFFSPNTPQFRLTDEAAK
LRLLAGAGLAGAVVMTFDKGRAQTSAQDFIHHDLIGRLGVSGIAVGYDFHFGKGRVGSPSLLASEAPKLGITVEVQPHVD
IDERPVSSTAIREALAEGQVAEATAMLGGPWFVTAEVIHGEKRGRDLGYPTANMRLDRDCGLRHGIYAVRVGRGRGANRV
LIDGVANFGRRPTFDNGAPLLETFLFDFKDSLYGEVLDVAFIGFIREEARFTSIEALIRQMDDDSARARAVLAANAGLFP
RLGEIG
>Mature_325_residues
PAFQIIRDATPPQHILKGAVVAMGNFDGVHLGHRAVIEAAIEMGRAKGRPALALTFEPHPRRFFSPNTPQFRLTDEAAKL
RLLAGAGLAGAVVMTFDKGRAQTSAQDFIHHDLIGRLGVSGIAVGYDFHFGKGRVGSPSLLASEAPKLGITVEVQPHVDI
DERPVSSTAIREALAEGQVAEATAMLGGPWFVTAEVIHGEKRGRDLGYPTANMRLDRDCGLRHGIYAVRVGRGRGANRVL
IDGVANFGRRPTFDNGAPLLETFLFDFKDSLYGEVLDVAFIGFIREEARFTSIEALIRQMDDDSARARAVLAANAGLFPR
LGEIG

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=293, Percent_Identity=38.9078498293515, Blast_Score=183, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004821
- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 35187; Mature: 35056

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAFQIIRDATPPQHILKGAVVAMGNFDGVHLGHRAVIEAAIEMGRAKGRPALALTFEPH
CCHHHHHHCCCCHHHHHCCCEEEECCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEECCC
PRRFFSPNTPQFRLTDEAAKLRLLAGAGLAGAVVMTFDKGRAQTSAQDFIHHDLIGRLGV
CCCCCCCCCCCEEEECHHHEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCC
SGIAVGYDFHFGKGRVGSPSLLASEAPKLGITVEVQPHVDIDERPVSSTAIREALAEGQV
CEEEEEEEEECCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCH
AEATAMLGGPWFVTAEVIHGEKRGRDLGYPTANMRLDRDCGLRHGIYAVRVGRGRGANRV
HHHHHHCCCCEEEEEEEECCHHCCCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCCCEE
LIDGVANFGRRPTFDNGAPLLETFLFDFKDSLYGEVLDVAFIGFIREEARFTSIEALIRQ
EEECHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDDDSARARAVLAANAGLFPRLGEIG
CCCCHHHHEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
PAFQIIRDATPPQHILKGAVVAMGNFDGVHLGHRAVIEAAIEMGRAKGRPALALTFEPH
CHHHHHHCCCCHHHHHCCCEEEECCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEECCC
PRRFFSPNTPQFRLTDEAAKLRLLAGAGLAGAVVMTFDKGRAQTSAQDFIHHDLIGRLGV
CCCCCCCCCCCEEEECHHHEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCC
SGIAVGYDFHFGKGRVGSPSLLASEAPKLGITVEVQPHVDIDERPVSSTAIREALAEGQV
CEEEEEEEEECCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCH
AEATAMLGGPWFVTAEVIHGEKRGRDLGYPTANMRLDRDCGLRHGIYAVRVGRGRGANRV
HHHHHHCCCCEEEEEEEECCHHCCCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCCCEE
LIDGVANFGRRPTFDNGAPLLETFLFDFKDSLYGEVLDVAFIGFIREEARFTSIEALIRQ
EEECHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDDDSARARAVLAANAGLFPRLGEIG
CCCCHHHHEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]