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The map label for this gene is purA [H]

Identifier: 146342761

GI number: 146342761

Start: 6151604

End: 6152896

Strand: Reverse

Name: purA [H]

Synonym: BRADO5935

Alternate gene names: 146342761

Gene position: 6152896-6151604 (Counterclockwise)

Preceding gene: 146342762

Following gene: 146342760

Centisome position: 82.52

GC content: 65.97

Gene sequence:

>1293_bases
ATGGCTAACGTGGTCGTCGTCGGCGCCCAATGGGGCGACGAGGGGAAGGGCAAGATCGTCGATTGGCTCTCGGAGCAGGC
CGACATCGTCGTGCGCTTCCAGGGCGGTCACAACGCCGGTCATACGCTCGTCATCAACGGCGCGACCTATAAGCTGGCGC
TGCTCCCGTCCGGCGTGCTGCGCTCCGGCAAGCTGTCGGTGATCGGCAATGGCGTGGTGTTCGATCCGCAGGCGTTCCTC
GACGAGGTCGGCAAGCTGCAGGCGCAGGGCCTGATCATCAGCCCGGACAATCTGCGCGTTGCCGAGAACGTCACGCTGAT
CCTGCCGCTGCATCGTGAGCTCGATGCGCTGAGGGAATCGTCCAACACCGGGACTGCCATCGGCACGACCCGCCGCGGCA
TCGGTCCGGCCTATGAGGACAAGGTCGGCCGCCGCGCGATCCGGCTGATGGATCTCGCCGATCTCGATACGCTGCCGCAC
AAGATCGATCGCCTGCTCGCTCATCACAACGCGCTGCGCCGGGGGCTCGGGCTGGAGCTGATCGACGGCGCCGAGATCCT
GCGCGAATTGACGGCGGTGGCGCCAAAGCTGTTGCCGTATGCGGAGACGGTTTGGCGGCTGCTCGACATCAAGCGCCGCG
AGGGCAAGCGCCTGCTGTTCGAGGGCGCCCAGGGCGCGCTGCTCGACGTCGATCACGGCACCTATCCTTATGTGACCTCG
TCCAACACGGTTGCGGCGCAAGCCGCGACCGGTGCCGGCCTGGGCCCGGGCGCGATCGGCTACGTGCTCGGCCTGTGCAA
GGCCTATACGACTCGCGTCGGCCAGGGACCTTTTCCCACCGAGCAGGATAACGAGACCGGCCGCAAGATCGGCGAGCGCG
GCCGTGAGTTCGGAACGAATACCGGCCGGCCGCGTCGTTGCGGCTGGTTCGACGCCGTCCTGGTCCGCCAGGCGGTGCGG
ACCTGTGGCATTAACGGTCTTGCGCTGACCAAGCTGGACATTCTCGACGGTTTCGACACCATCGAGGTCTGCACCGGCTA
CAGGCTGGACGGCAAGGAGATCGATCATTTCCCGGCAGGCGAAGGTGCCCAGGCCCGGGTCGAGCCGATCTACGAGACCA
TCGAAGGCTGGAAGCAGCCGACGGCGAACGCGCGCTCCTGGGCCGATCTGCCGGCCCAGGCCATCAAATACGTCCGGCGC
ATCGAGGAACTGGTGGGTTGCCCAATTGCGCTGCTTTCCACCAGTCCCGAACGCGAGGATACTATCCTGGTCCAGAATCC
CTTCGAGGCGTGA

Upstream 100 bases:

>100_bases
TGGGCTGGGCGCATGGTTGGCCAGGCTGGCCCCTCATCGGAAGCGATATCGACCGAGCCGATCGTGCTGTTTTGGATCTT
GGCAGAGGAAAGAGCTGCGA

Downstream 100 bases:

>100_bases
GGGGACCCGACAGCGCAGCCCGCGAATTGAGTTGATATGGCCGACTACTACCCGCTGATAGCCCGCGCCATTGCCGGATT
GGACCCCAACGCTCCCGGCG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MANVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLVINGATYKLALLPSGVLRSGKLSVIGNGVVFDPQAFL
DEVGKLQAQGLIISPDNLRVAENVTLILPLHRELDALRESSNTGTAIGTTRRGIGPAYEDKVGRRAIRLMDLADLDTLPH
KIDRLLAHHNALRRGLGLELIDGAEILRELTAVAPKLLPYAETVWRLLDIKRREGKRLLFEGAQGALLDVDHGTYPYVTS
SNTVAAQAATGAGLGPGAIGYVLGLCKAYTTRVGQGPFPTEQDNETGRKIGERGREFGTNTGRPRRCGWFDAVLVRQAVR
TCGINGLALTKLDILDGFDTIEVCTGYRLDGKEIDHFPAGEGAQARVEPIYETIEGWKQPTANARSWADLPAQAIKYVRR
IEELVGCPIALLSTSPEREDTILVQNPFEA

Sequences:

>Translated_430_residues
MANVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLVINGATYKLALLPSGVLRSGKLSVIGNGVVFDPQAFL
DEVGKLQAQGLIISPDNLRVAENVTLILPLHRELDALRESSNTGTAIGTTRRGIGPAYEDKVGRRAIRLMDLADLDTLPH
KIDRLLAHHNALRRGLGLELIDGAEILRELTAVAPKLLPYAETVWRLLDIKRREGKRLLFEGAQGALLDVDHGTYPYVTS
SNTVAAQAATGAGLGPGAIGYVLGLCKAYTTRVGQGPFPTEQDNETGRKIGERGREFGTNTGRPRRCGWFDAVLVRQAVR
TCGINGLALTKLDILDGFDTIEVCTGYRLDGKEIDHFPAGEGAQARVEPIYETIEGWKQPTANARSWADLPAQAIKYVRR
IEELVGCPIALLSTSPEREDTILVQNPFEA
>Mature_429_residues
ANVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLVINGATYKLALLPSGVLRSGKLSVIGNGVVFDPQAFLD
EVGKLQAQGLIISPDNLRVAENVTLILPLHRELDALRESSNTGTAIGTTRRGIGPAYEDKVGRRAIRLMDLADLDTLPHK
IDRLLAHHNALRRGLGLELIDGAEILRELTAVAPKLLPYAETVWRLLDIKRREGKRLLFEGAQGALLDVDHGTYPYVTSS
NTVAAQAATGAGLGPGAIGYVLGLCKAYTTRVGQGPFPTEQDNETGRKIGERGREFGTNTGRPRRCGWFDAVLVRQAVRT
CGINGLALTKLDILDGFDTIEVCTGYRLDGKEIDHFPAGEGAQARVEPIYETIEGWKQPTANARSWADLPAQAIKYVRRI
EELVGCPIALLSTSPEREDTILVQNPFEA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=423, Percent_Identity=43.4988179669031, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=42.5882352941177, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=41.3705583756345, Blast_Score=295, Evalue=6e-80,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=50.9302325581395, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25146553, Length=428, Percent_Identity=42.0560747663551, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=428, Percent_Identity=42.0560747663551, Blast_Score=329, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=40.6032482598608, Blast_Score=313, Evalue=3e-86,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=42.5531914893617, Blast_Score=329, Evalue=2e-90,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46604; Mature: 46473

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLVINGATYKLALLPSGVL
CCCEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEEECCEEEEEECCCCHH
RSGKLSVIGNGVVFDPQAFLDEVGKLQAQGLIISPDNLRVAENVTLILPLHRELDALRES
CCCCEEEEECCEEECHHHHHHHHHHHHHCEEEECCCCEEEECCEEEEEECHHHHHHHHHC
SNTGTAIGTTRRGIGPAYEDKVGRRAIRLMDLADLDTLPHKIDRLLAHHNALRRGLGLEL
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IDGAEILRELTAVAPKLLPYAETVWRLLDIKRREGKRLLFEGAQGALLDVDHGTYPYVTS
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNTVAAQAATGAGLGPGAIGYVLGLCKAYTTRVGQGPFPTEQDNETGRKIGERGREFGTN
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
TGRPRRCGWFDAVLVRQAVRTCGINGLALTKLDILDGFDTIEVCTGYRLDGKEIDHFPAG
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCHHHHHCCCCCCCCCCCCCCCC
EGAQARVEPIYETIEGWKQPTANARSWADLPAQAIKYVRRIEELVGCPIALLSTSPERED
CCCCCHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
TILVQNPFEA
EEEECCCCCC
>Mature Secondary Structure 
ANVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLVINGATYKLALLPSGVL
CCEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEEECCEEEEEECCCCHH
RSGKLSVIGNGVVFDPQAFLDEVGKLQAQGLIISPDNLRVAENVTLILPLHRELDALRES
CCCCEEEEECCEEECHHHHHHHHHHHHHCEEEECCCCEEEECCEEEEEECHHHHHHHHHC
SNTGTAIGTTRRGIGPAYEDKVGRRAIRLMDLADLDTLPHKIDRLLAHHNALRRGLGLEL
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IDGAEILRELTAVAPKLLPYAETVWRLLDIKRREGKRLLFEGAQGALLDVDHGTYPYVTS
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNTVAAQAATGAGLGPGAIGYVLGLCKAYTTRVGQGPFPTEQDNETGRKIGERGREFGTN
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
TGRPRRCGWFDAVLVRQAVRTCGINGLALTKLDILDGFDTIEVCTGYRLDGKEIDHFPAG
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCHHHHHCCCCCCCCCCCCCCCC
EGAQARVEPIYETIEGWKQPTANARSWADLPAQAIKYVRRIEELVGCPIALLSTSPERED
CCCCCHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
TILVQNPFEA
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]