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The map label for this gene is ureF1 [H]

Identifier: 146342698

GI number: 146342698

Start: 6084244

End: 6084915

Strand: Reverse

Name: ureF1 [H]

Synonym: BRADO5865

Alternate gene names: 146342698

Gene position: 6084915-6084244 (Counterclockwise)

Preceding gene: 146342699

Following gene: 146342697

Centisome position: 81.6

GC content: 69.49

Gene sequence:

>672_bases
ATGCTGCCGATGTTGCGCGCGATCTGGCAGGCCGACGCCGCGTTTCCAAGCGGCGCCTTCGCATTCTCCTACGGGATCGA
AGGCGCGTTGTCGCTGCGCCCCGACATGACCCTGCATGATTTCGAGCGGCTTTGTGCCGCGGTGCTGCGGCAGCGCTGGG
CGAGCTATGACCGGGTGGCTCTGCTGCGCGCGTTCCGGACCGGTCCCGGGCTGGACGCACTCGCGACGATCGACCGGCAG
GTCGAAGCGTCGACGGTGATCGAGACGCTGCGCGCCGGCTCGCGCCGCAACGGCGCATCGTTCCTGGCGGCGCATGCGCG
GCTCGGTCAGCCCCTGGCGGTCGATCTGCGGGAGGCCGTCCGGGCAGGGAAGTGTCTCGGGCACATCGCCGTGATGCAGG
GCGCGCTGTGGGCGGCGCTCGGACTCGATCAGCGTCATGCCGAATTGTGCTCGGCCTATACGGTCGCGTCCGGACTGATC
ACGGCCGCGGTGCGGCTCGGCGCCGTCGGCGCGCTGCAGGGACAGGGTGTCTTGCAGGCGCTGCTGCCGCTGATCGACGA
TCTGCTCGCGACGCCGGTCGACGACGAGGCCGAGCTCGAAAGCTTCATCCCCTTTCTCGACGTCGCGACGGCGCGACACG
TCCACGCCGAGCTGCGCCTGTTTGCCAATTGA

Upstream 100 bases:

>100_bases
TCACCGTGCTCGACGAGGAGTCCGCCGATAGCCCGGCGCAGGCCGACGCTGCACCCGGAAGCGGACATGGTCACCATCAT
CACCACGGCGATCACTGACC

Downstream 100 bases:

>100_bases
GAGCGAGACCGTATGCTGCTGACACCCACCGAACTCGAGCGCCTCACCATCTTCAACGCCGCCGAGCTGTCGCGCCGCCG
CCGCGGCCGCGGACTCAAGC

Product: putative urease accessory protein UreF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MLPMLRAIWQADAAFPSGAFAFSYGIEGALSLRPDMTLHDFERLCAAVLRQRWASYDRVALLRAFRTGPGLDALATIDRQ
VEASTVIETLRAGSRRNGASFLAAHARLGQPLAVDLREAVRAGKCLGHIAVMQGALWAALGLDQRHAELCSAYTVASGLI
TAAVRLGAVGALQGQGVLQALLPLIDDLLATPVDDEAELESFIPFLDVATARHVHAELRLFAN

Sequences:

>Translated_223_residues
MLPMLRAIWQADAAFPSGAFAFSYGIEGALSLRPDMTLHDFERLCAAVLRQRWASYDRVALLRAFRTGPGLDALATIDRQ
VEASTVIETLRAGSRRNGASFLAAHARLGQPLAVDLREAVRAGKCLGHIAVMQGALWAALGLDQRHAELCSAYTVASGLI
TAAVRLGAVGALQGQGVLQALLPLIDDLLATPVDDEAELESFIPFLDVATARHVHAELRLFAN
>Mature_223_residues
MLPMLRAIWQADAAFPSGAFAFSYGIEGALSLRPDMTLHDFERLCAAVLRQRWASYDRVALLRAFRTGPGLDALATIDRQ
VEASTVIETLRAGSRRNGASFLAAHARLGQPLAVDLREAVRAGKCLGHIAVMQGALWAALGLDQRHAELCSAYTVASGLI
TAAVRLGAVGALQGQGVLQALLPLIDDLLATPVDDEAELESFIPFLDVATARHVHAELRLFAN

Specific function: Required for maturation of urease via the functional incorporation of the urease nickel metallocenter [H]

COG id: COG0830

COG function: function code O; Urease accessory protein UreF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ureF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002639 [H]

Pfam domain/function: PF01730 UreF [H]

EC number: NA

Molecular weight: Translated: 23895; Mature: 23895

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPMLRAIWQADAAFPSGAFAFSYGIEGALSLRPDMTLHDFERLCAAVLRQRWASYDRVA
CCHHHHHHHHHHCCCCCCCCEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
LLRAFRTGPGLDALATIDRQVEASTVIETLRAGSRRNGASFLAAHARLGQPLAVDLREAV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
RAGKCLGHIAVMQGALWAALGLDQRHAELCSAYTVASGLITAAVRLGAVGALQGQGVLQA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LLPLIDDLLATPVDDEAELESFIPFLDVATARHVHAELRLFAN
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPMLRAIWQADAAFPSGAFAFSYGIEGALSLRPDMTLHDFERLCAAVLRQRWASYDRVA
CCHHHHHHHHHHCCCCCCCCEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
LLRAFRTGPGLDALATIDRQVEASTVIETLRAGSRRNGASFLAAHARLGQPLAVDLREAV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
RAGKCLGHIAVMQGALWAALGLDQRHAELCSAYTVASGLITAAVRLGAVGALQGQGVLQA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LLPLIDDLLATPVDDEAELESFIPFLDVATARHVHAELRLFAN
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]