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The map label for this gene is bam [H]

Identifier: 146342624

GI number: 146342624

Start: 5990513

End: 5991907

Strand: Reverse

Name: bam [H]

Synonym: BRADO5789

Alternate gene names: 146342624

Gene position: 5991907-5990513 (Counterclockwise)

Preceding gene: 146342627

Following gene: 146342623

Centisome position: 80.36

GC content: 71.61

Gene sequence:

>1395_bases
ATGACCGATCTCTGGCGCCTGCCGGCTGCCGACCTCGCCGCCCTCATCAAGTCGAAACAGGTCTCCGCCCGCGACGCCGC
CACGGCCGCGCTGGCGCGGCTCGATGCGGTGAACCCCGCGATCAACGCGGTCATCGACCATCGTCCCGAGGACGTGCTGC
AGCAGGCCGCCGCGATCGATGCGCGTCTGGCGCGCGGCGAGGATCCCGGGCCGCTTGCCGGCGTGCCGGTGACGATCAAG
GTCAATGTCGACCAGCAGGGCTACGCCACCACCAACGGCCTCAATCTGCAGAAGGACGCGATCGCGACCGCGCACAACCC
CGTCGTGAACAATTTCGTCAAGGCCGGCGCCGTGCTGCTCGGCCGCACCAACACCCCGGCGGTGTCCTATCGCTGGTTCA
CCAGCAACCTCATCCACGGCCACACCAGGAATCCGCGCGACCCCGGCATCACCCCGGGCGGCTCCTCCGGCGGCGCCGGC
GCGGCCACCGCCGCCGGCATCGGCCATATCGGCCACGGCACCGACATCGCCGGCTCCGTCCGCTATCCGGCCTATGCCTG
CGGCATCCACGGCCTGCGCCCGACGGTCGGCCGCATCCCCGCCTTCAACACCTGCCTGCCCGAGCGTCCGATCGGCCCGC
AGATCAGCGCCGTCTCCGGCCCGCTCGCCCGCACGGTCAACGACGTCAGGATCGCGCTGGCCGCGATGTCGGCGCGCGAC
GTCCGCGATGTCTGGTGGATGCCGGTGCCGCTGGAGGGCCCGCCCTTGCCGAAGCGCGTCACGCTCTGCGTCAATCCCGA
CGGCCTCAACCCGGTGCCCGAAGTGGTCGCCGCGCTGCGCGATGCCGCCAGGCGGCTCGAAGCCGCCGGCTGGACCGTCG
AGGAGATCCAGGACACGCCGCCGATGCAGGAGGCCGCGCTGCTGCAGACGCGGCTCTGGCTCGGCGACGGCTACGAGGCG
CAGCTCGCCGCGGCCGAGAAGGAGGGCGATCCCGGCGCGCTCGCCTGCCTGCGCGGAAACCGTGAGAAGGTGCACCCGTT
CGACCTCTCGGCGACGCTCGCACGCCGCGCCGCGCTGACCCGGCAATGGCTCCTGTTCATGGAGCAGCATCCGCTGCTGC
TGCTGCCCGTCTCCGGCATGCTGCCCTTCGCCGACCAGCTCGACCGCAAGGACGAGGAGTCGTTCAAGCAGGTCTGGCAC
GCCCAGCTCACGCAGATCGCGATTCCGTTCTTCAGCCTGCCGGGCCTCGTCGTCTCCACCGGCATGGTCGGCCGCGTCCC
CGTCGGCGTCCAGCTCGTCGGCCAGCGGTTTCGCGAGGACATGTGCCTTGCCGCCGGCGAAGCCATCGAGGCCGGCGGCA
CCCCGCCGTCGCCGATCGATCCGGTGCTAGGCTGA

Upstream 100 bases:

>100_bases
CATGCGTTTTGCTGCATAGGCCCGTCAGAGAGCCGCGTGCGCGCCGCTTTCTCCGTCCTGTCCGCCGCGCTAAGAACGCG
GGACCACTACGGAGCCCCCC

Downstream 100 bases:

>100_bases
TGGCGAGCATCTACGATTTCACCGCATCCTCGCTGACCGGCGAGGAGGTGCCGCTGAACCGCTTCGAGGGCCAGGTGCTC
TTGATCGTCAACACCGCCAG

Product: amidase

Products: NA

Alternate protein names: IAH; Indole-3-acetamide hydrolase [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTDLWRLPAADLAALIKSKQVSARDAATAALARLDAVNPAINAVIDHRPEDVLQQAAAIDARLARGEDPGPLAGVPVTIK
VNVDQQGYATTNGLNLQKDAIATAHNPVVNNFVKAGAVLLGRTNTPAVSYRWFTSNLIHGHTRNPRDPGITPGGSSGGAG
AATAAGIGHIGHGTDIAGSVRYPAYACGIHGLRPTVGRIPAFNTCLPERPIGPQISAVSGPLARTVNDVRIALAAMSARD
VRDVWWMPVPLEGPPLPKRVTLCVNPDGLNPVPEVVAALRDAARRLEAAGWTVEEIQDTPPMQEAALLQTRLWLGDGYEA
QLAAAEKEGDPGALACLRGNREKVHPFDLSATLARRAALTRQWLLFMEQHPLLLLPVSGMLPFADQLDRKDEESFKQVWH
AQLTQIAIPFFSLPGLVVSTGMVGRVPVGVQLVGQRFREDMCLAAGEAIEAGGTPPSPIDPVLG

Sequences:

>Translated_464_residues
MTDLWRLPAADLAALIKSKQVSARDAATAALARLDAVNPAINAVIDHRPEDVLQQAAAIDARLARGEDPGPLAGVPVTIK
VNVDQQGYATTNGLNLQKDAIATAHNPVVNNFVKAGAVLLGRTNTPAVSYRWFTSNLIHGHTRNPRDPGITPGGSSGGAG
AATAAGIGHIGHGTDIAGSVRYPAYACGIHGLRPTVGRIPAFNTCLPERPIGPQISAVSGPLARTVNDVRIALAAMSARD
VRDVWWMPVPLEGPPLPKRVTLCVNPDGLNPVPEVVAALRDAARRLEAAGWTVEEIQDTPPMQEAALLQTRLWLGDGYEA
QLAAAEKEGDPGALACLRGNREKVHPFDLSATLARRAALTRQWLLFMEQHPLLLLPVSGMLPFADQLDRKDEESFKQVWH
AQLTQIAIPFFSLPGLVVSTGMVGRVPVGVQLVGQRFREDMCLAAGEAIEAGGTPPSPIDPVLG
>Mature_463_residues
TDLWRLPAADLAALIKSKQVSARDAATAALARLDAVNPAINAVIDHRPEDVLQQAAAIDARLARGEDPGPLAGVPVTIKV
NVDQQGYATTNGLNLQKDAIATAHNPVVNNFVKAGAVLLGRTNTPAVSYRWFTSNLIHGHTRNPRDPGITPGGSSGGAGA
ATAAGIGHIGHGTDIAGSVRYPAYACGIHGLRPTVGRIPAFNTCLPERPIGPQISAVSGPLARTVNDVRIALAAMSARDV
RDVWWMPVPLEGPPLPKRVTLCVNPDGLNPVPEVVAALRDAARRLEAAGWTVEEIQDTPPMQEAALLQTRLWLGDGYEAQ
LAAAEKEGDPGALACLRGNREKVHPFDLSATLARRAALTRQWLLFMEQHPLLLLPVSGMLPFADQLDRKDEESFKQVWHA
QLTQIAIPFFSLPGLVVSTGMVGRVPVGVQLVGQRFREDMCLAAGEAIEAGGTPPSPIDPVLG

Specific function: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid (IAA) [H]

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=247, Percent_Identity=35.2226720647773, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=23.8, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI166795287, Length=271, Percent_Identity=31.7343173431734, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=510, Percent_Identity=25.8823529411765, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17538252, Length=253, Percent_Identity=33.201581027668, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17556278, Length=379, Percent_Identity=30.0791556728232, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17556276, Length=379, Percent_Identity=30.0791556728232, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17556264, Length=191, Percent_Identity=34.5549738219895, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=212, Percent_Identity=31.6037735849057, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17538254, Length=227, Percent_Identity=28.1938325991189, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320448, Length=225, Percent_Identity=27.5555555555556, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550774, Length=201, Percent_Identity=37.8109452736318, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24648435, Length=201, Percent_Identity=37.8109452736318, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24648437, Length=201, Percent_Identity=37.8109452736318, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24652985, Length=503, Percent_Identity=26.441351888668, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI19922090, Length=503, Percent_Identity=26.441351888668, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24652981, Length=503, Percent_Identity=26.441351888668, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24652983, Length=503, Percent_Identity=26.441351888668, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI21356731, Length=228, Percent_Identity=33.3333333333333, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24644968, Length=507, Percent_Identity=22.879684418146, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078093, Length=311, Percent_Identity=27.6527331189711, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648441, Length=129, Percent_Identity=40.3100775193798, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648439, Length=129, Percent_Identity=40.3100775193798, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 49186; Mature: 49055

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLWRLPAADLAALIKSKQVSARDAATAALARLDAVNPAINAVIDHRPEDVLQQAAAID
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHH
ARLARGEDPGPLAGVPVTIKVNVDQQGYATTNGLNLQKDAIATAHNPVVNNFVKAGAVLL
HHHHCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCHHHHHHCCCHHHHHHHHHCEEEE
GRTNTPAVSYRWFTSNLIHGHTRNPRDPGITPGGSSGGAGAATAAGIGHIGHGTDIAGSV
ECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCC
RYPAYACGIHGLRPTVGRIPAFNTCLPERPIGPQISAVSGPLARTVNDVRIALAAMSARD
CCCHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCC
VRDVWWMPVPLEGPPLPKRVTLCVNPDGLNPVPEVVAALRDAARRLEAAGWTVEEIQDTP
HHHHCEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
PMQEAALLQTRLWLGDGYEAQLAAAEKEGDPGALACLRGNREKVHPFDLSATLARRAALT
CHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
RQWLLFMEQHPLLLLPVSGMLPFADQLDRKDEESFKQVWHAQLTQIAIPFFSLPGLVVST
HHHHHHHHCCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
GMVGRVPVGVQLVGQRFREDMCLAAGEAIEAGGTPPSPIDPVLG
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDLWRLPAADLAALIKSKQVSARDAATAALARLDAVNPAINAVIDHRPEDVLQQAAAID
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHH
ARLARGEDPGPLAGVPVTIKVNVDQQGYATTNGLNLQKDAIATAHNPVVNNFVKAGAVLL
HHHHCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCHHHHHHCCCHHHHHHHHHCEEEE
GRTNTPAVSYRWFTSNLIHGHTRNPRDPGITPGGSSGGAGAATAAGIGHIGHGTDIAGSV
ECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCC
RYPAYACGIHGLRPTVGRIPAFNTCLPERPIGPQISAVSGPLARTVNDVRIALAAMSARD
CCCHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCC
VRDVWWMPVPLEGPPLPKRVTLCVNPDGLNPVPEVVAALRDAARRLEAAGWTVEEIQDTP
HHHHCEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
PMQEAALLQTRLWLGDGYEAQLAAAEKEGDPGALACLRGNREKVHPFDLSATLARRAALT
CHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
RQWLLFMEQHPLLLLPVSGMLPFADQLDRKDEESFKQVWHAQLTQIAIPFFSLPGLVVST
HHHHHHHHCCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
GMVGRVPVGVQLVGQRFREDMCLAAGEAIEAGGTPPSPIDPVLG
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]