Click here to switch to the map view.

The map label for this gene is mltB [H]

Identifier: 146342573

GI number: 146342573

Start: 5932649

End: 5933887

Strand: Reverse

Name: mltB [H]

Synonym: BRADO5736

Alternate gene names: 146342573

Gene position: 5933887-5932649 (Counterclockwise)

Preceding gene: 146342574

Following gene: 146342570

Centisome position: 79.58

GC content: 67.23

Gene sequence:

>1239_bases
GTGCCGCGTCTGCTTCGCCAGTGCCTCGCGGTTTTCCTGTTCGGCGTCCTCCTGTGCCATGCCGGCTTGGCGACCGCCGC
CGACGCCGCCTTCACCCAATTCGTCGCGTCGCTCTGGCCCGAAGCCAAGGACCAGGGCGTTTCGCGCGCCACCTTCGAGT
CCGAGACGCGCGGGCTTGAGCCCGACTATAAGCTTCCCGATCTGCTGCTGCCCGGCCGGCCTGCAACCGGCGCACCGGCG
CAAGCCGAGTTCGTGCAGGTGCCGGCCGACTACATCAAGGAAGCGTCGATCGCACGGCTGGCCGCGCATGGCCAGACGCT
GATCCTGCAATACCGGCCGGCGCTGGCCGAGATCGAGCGCCGCTTCGGCGTGCCTGGTCCGATCGTGCTCGCGATCTGGG
GCCGCGAGACCGATTTCGGCCGCTACGCGTTGCCCTATGACGCGCTGCGCGTGCTGGCGACCCAGGCCTATGTCGGACGG
CGCAAGGAGCAGTACCGCACCGAGTTCATCATGGCGCTGAAGATGCTGAGCGATGGCGTGGTGTCGCGCCGCGATCTCAG
GGCGTCATGGGGCGGCGCGGTCGGCCTGACCCAGTTCCTTCCCTCGGAGTACTACAAGCACGGCGTCGATCTCGACGGCG
ACGGCAAGGTCGATCTCTGGCACTCGGTGCCGGATGCGCTGGGCTCGGCGGCCAAGCAACTCGCCAACAAGGGCTGGCAG
AGCGGCGTGCGCTGGGCCTACGAGGTCAAGCCGCCCGCCAACGTCGATTGCACGATGGGCGTGCCTGAGTTGAAGAAGCC
GATCGCGGAGTGGCTGCGGGCCGGATTCGCGCCGGTGCGCGGCCAGCGGCTGAGCGCCGCCGAGCAGCAGCAGCCGGCGT
CCCTGCTGCAGCCCGAAGGCATCTATGGCCCGTCATTCCTGACGACCGCAAACTACTTCGTCATCAAGGAATACAATTTC
TCGGATCTCTACGTGCTGTTCGTTGGCCATCTCGCCGACCGGATGACCAGCCCGGCCGGCTTCGCGACGCCGTGGTCGGC
TTCCCAGCAATTGCGGAGCAAGGATGTGGAGTCGATGCAGAAGGGTCTCACGCGCCTCGGGCTCTACGCGGACAAGCTCG
ACGGCAAGGCGGGGATGCAGACGCGTGCCGCGCTCGGCACGTTTCAGAAGAACGCCGGCTTGAAGGTGGATTGCTGGCCG
AGCGAAGCCGTGCTGCAGGCGATCAACGCCTCGCGCTGA

Upstream 100 bases:

>100_bases
CTCGAAAATCAGTACGCCAGCATGTCCAATCGGCTGGACCGGATGGACCTGAGAATAGAGCACATCGAACGGCGGCTTGG
CCTTCAGGATGCCTGATCAG

Downstream 100 bases:

>100_bases
CGGCCTGTGCGGCCATCGGACGCAGCCAGCAAAAAGCCCGGCCGCAGGGCCGGGCTCTGCCGCATGGAGATGCGAGGTTT
CGATCAGTAGCAGACGCGAA

Product: putative transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPRLLRQCLAVFLFGVLLCHAGLATAADAAFTQFVASLWPEAKDQGVSRATFESETRGLEPDYKLPDLLLPGRPATGAPA
QAEFVQVPADYIKEASIARLAAHGQTLILQYRPALAEIERRFGVPGPIVLAIWGRETDFGRYALPYDALRVLATQAYVGR
RKEQYRTEFIMALKMLSDGVVSRRDLRASWGGAVGLTQFLPSEYYKHGVDLDGDGKVDLWHSVPDALGSAAKQLANKGWQ
SGVRWAYEVKPPANVDCTMGVPELKKPIAEWLRAGFAPVRGQRLSAAEQQQPASLLQPEGIYGPSFLTTANYFVIKEYNF
SDLYVLFVGHLADRMTSPAGFATPWSASQQLRSKDVESMQKGLTRLGLYADKLDGKAGMQTRAALGTFQKNAGLKVDCWP
SEAVLQAINASR

Sequences:

>Translated_412_residues
MPRLLRQCLAVFLFGVLLCHAGLATAADAAFTQFVASLWPEAKDQGVSRATFESETRGLEPDYKLPDLLLPGRPATGAPA
QAEFVQVPADYIKEASIARLAAHGQTLILQYRPALAEIERRFGVPGPIVLAIWGRETDFGRYALPYDALRVLATQAYVGR
RKEQYRTEFIMALKMLSDGVVSRRDLRASWGGAVGLTQFLPSEYYKHGVDLDGDGKVDLWHSVPDALGSAAKQLANKGWQ
SGVRWAYEVKPPANVDCTMGVPELKKPIAEWLRAGFAPVRGQRLSAAEQQQPASLLQPEGIYGPSFLTTANYFVIKEYNF
SDLYVLFVGHLADRMTSPAGFATPWSASQQLRSKDVESMQKGLTRLGLYADKLDGKAGMQTRAALGTFQKNAGLKVDCWP
SEAVLQAINASR
>Mature_411_residues
PRLLRQCLAVFLFGVLLCHAGLATAADAAFTQFVASLWPEAKDQGVSRATFESETRGLEPDYKLPDLLLPGRPATGAPAQ
AEFVQVPADYIKEASIARLAAHGQTLILQYRPALAEIERRFGVPGPIVLAIWGRETDFGRYALPYDALRVLATQAYVGRR
KEQYRTEFIMALKMLSDGVVSRRDLRASWGGAVGLTQFLPSEYYKHGVDLDGDGKVDLWHSVPDALGSAAKQLANKGWQS
GVRWAYEVKPPANVDCTMGVPELKKPIAEWLRAGFAPVRGQRLSAAEQQQPASLLQPEGIYGPSFLTTANYFVIKEYNFS
DLYVLFVGHLADRMTSPAGFATPWSASQQLRSKDVESMQKGLTRLGLYADKLDGKAGMQTRAALGTFQKNAGLKVDCWPS
EAVLQAINASR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=235, Percent_Identity=32.7659574468085, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 45182; Mature: 45051

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLLRQCLAVFLFGVLLCHAGLATAADAAFTQFVASLWPEAKDQGVSRATFESETRGLE
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHCCCC
PDYKLPDLLLPGRPATGAPAQAEFVQVPADYIKEASIARLAAHGQTLILQYRPALAEIER
CCCCCCCCCCCCCCCCCCCCCHHHEECCHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHH
RFGVPGPIVLAIWGRETDFGRYALPYDALRVLATQAYVGRRKEQYRTEFIMALKMLSDGV
HHCCCCCEEEEEECCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VSRRDLRASWGGAVGLTQFLPSEYYKHGVDLDGDGKVDLWHSVPDALGSAAKQLANKGWQ
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
SGVRWAYEVKPPANVDCTMGVPELKKPIAEWLRAGFAPVRGQRLSAAEQQQPASLLQPEG
HCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHCCCCC
IYGPSFLTTANYFVIKEYNFSDLYVLFVGHLADRMTSPAGFATPWSASQQLRSKDVESMQ
CCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
KGLTRLGLYADKLDGKAGMQTRAALGTFQKNAGLKVDCWPSEAVLQAINASR
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCC
>Mature Secondary Structure 
PRLLRQCLAVFLFGVLLCHAGLATAADAAFTQFVASLWPEAKDQGVSRATFESETRGLE
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHCCCC
PDYKLPDLLLPGRPATGAPAQAEFVQVPADYIKEASIARLAAHGQTLILQYRPALAEIER
CCCCCCCCCCCCCCCCCCCCCHHHEECCHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHH
RFGVPGPIVLAIWGRETDFGRYALPYDALRVLATQAYVGRRKEQYRTEFIMALKMLSDGV
HHCCCCCEEEEEECCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VSRRDLRASWGGAVGLTQFLPSEYYKHGVDLDGDGKVDLWHSVPDALGSAAKQLANKGWQ
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
SGVRWAYEVKPPANVDCTMGVPELKKPIAEWLRAGFAPVRGQRLSAAEQQQPASLLQPEG
HCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHCCCCC
IYGPSFLTTANYFVIKEYNFSDLYVLFVGHLADRMTSPAGFATPWSASQQLRSKDVESMQ
CCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
KGLTRLGLYADKLDGKAGMQTRAALGTFQKNAGLKVDCWPSEAVLQAINASR
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]