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The map label for this gene is phnD [H]

Identifier: 146342213

GI number: 146342213

Start: 5571728

End: 5572660

Strand: Reverse

Name: phnD [H]

Synonym: BRADO5365

Alternate gene names: 146342213

Gene position: 5572660-5571728 (Counterclockwise)

Preceding gene: 146342214

Following gene: 146342212

Centisome position: 74.73

GC content: 63.13

Gene sequence:

>933_bases
ATGATCAATCGTCGCATCGTTCTGGCGGGCGTCGCTGCGCTGGCTTTCACGGCCCAGGCGTCGGCCGAGGATTGGAAGGC
GAAGTATCCTGAACTGACCTTCGCTGTGGTCCCGGCGGAGAATGCCTCGGGCGTGACCGAGCGCTGGACGCCGTTCGTCA
ACTATCTGTCCAAGGAGCTGGGCGTGAAGGTCACGCTCCGCATCGCCAACGACTACGCCGCCGTCATCGAGGGCCAGCGC
GCCGGCAACATCCACATCGCGAGCTACGGCTCGGCCTCGTTCGCCCGCGCCCGTTTGACGGGCGTCAAGACGGACGCCTT
CGCCAACGACATCAACGCCGACGGCAGCACCGGCTACTACTCGGTGTTCTTCGTCAAGGCCAACAGCCCCTACAAGAAGG
TCGAGGATCTCAAGGGCAAAAACCTCGGCCTGGTCGATCCGAACTCGACCTCCGGCAACAACGTGCCGCGCTTCGAGCTC
AACAAGATGGGCGTGACCGACGCCGAGGCCTATTTCGGCAAGGTCGTGTTCACCGGCAGCCACGAGAACGCGGTTCTCGC
CCTCAGCCAGGGCACCGTCGACGTCGCCGCCAACCAGTGGACCAGCGACAACGACTCGACGCTCGCGCAGATGCTCACCA
AGGGCATGCTGAAGAACGCAGACGGCTCGCCGATGAAGAAGGACGACTTCCGCATCATCCACAAGTCGGCCCCGATCATC
AACGGCCCCTATGCCTACAACACCGATCTGCCTGAAGACGCCAAACAGGCGATCGCCAAGGCCTTCTTCGACGCGCCGAC
CAAGGACAAGGCGGCGTTCGACCGTCTCTCCGACGGCCAGAAGAAGGGCTTCCATCCGGCCACGACCAAGGACTGGGACG
GCACGATCGATCTGATCAAGTTCGTCGACAGCCTGCGCAAGAAGGCGTCCTGA

Upstream 100 bases:

>100_bases
TCGGCGAAGCCGCCGTGGCCTGAGATCTTGGTTTCTACCGTCACGGGCTCCGCATCGCGGGGCGCGAGACTCGTTACTTA
CTGCAACAAGAGGGAATGTT

Downstream 100 bases:

>100_bases
TCGACGTCCCATCCAAGCGGGAGCCGGGTCGGGCATGACCCGGCTCTCGCCTTGACCAGAGCCAGATCGTTTCACAGAGA
GAGCAGCACGCATCCGATGG

Product: putative PhnD protein, phosphonate ABC transporter exported protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MINRRIVLAGVAALAFTAQASAEDWKAKYPELTFAVVPAENASGVTERWTPFVNYLSKELGVKVTLRIANDYAAVIEGQR
AGNIHIASYGSASFARARLTGVKTDAFANDINADGSTGYYSVFFVKANSPYKKVEDLKGKNLGLVDPNSTSGNNVPRFEL
NKMGVTDAEAYFGKVVFTGSHENAVLALSQGTVDVAANQWTSDNDSTLAQMLTKGMLKNADGSPMKKDDFRIIHKSAPII
NGPYAYNTDLPEDAKQAIAKAFFDAPTKDKAAFDRLSDGQKKGFHPATTKDWDGTIDLIKFVDSLRKKAS

Sequences:

>Translated_310_residues
MINRRIVLAGVAALAFTAQASAEDWKAKYPELTFAVVPAENASGVTERWTPFVNYLSKELGVKVTLRIANDYAAVIEGQR
AGNIHIASYGSASFARARLTGVKTDAFANDINADGSTGYYSVFFVKANSPYKKVEDLKGKNLGLVDPNSTSGNNVPRFEL
NKMGVTDAEAYFGKVVFTGSHENAVLALSQGTVDVAANQWTSDNDSTLAQMLTKGMLKNADGSPMKKDDFRIIHKSAPII
NGPYAYNTDLPEDAKQAIAKAFFDAPTKDKAAFDRLSDGQKKGFHPATTKDWDGTIDLIKFVDSLRKKAS
>Mature_310_residues
MINRRIVLAGVAALAFTAQASAEDWKAKYPELTFAVVPAENASGVTERWTPFVNYLSKELGVKVTLRIANDYAAVIEGQR
AGNIHIASYGSASFARARLTGVKTDAFANDINADGSTGYYSVFFVKANSPYKKVEDLKGKNLGLVDPNSTSGNNVPRFEL
NKMGVTDAEAYFGKVVFTGSHENAVLALSQGTVDVAANQWTSDNDSTLAQMLTKGMLKNADGSPMKKDDFRIIHKSAPII
NGPYAYNTDLPEDAKQAIAKAFFDAPTKDKAAFDRLSDGQKKGFHPATTKDWDGTIDLIKFVDSLRKKAS

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=286, Percent_Identity=33.2167832167832, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017797
- InterPro:   IPR005770 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 33578; Mature: 33578

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINRRIVLAGVAALAFTAQASAEDWKAKYPELTFAVVPAENASGVTERWTPFVNYLSKEL
CCCCEEEEHHHHHHHHHHCCCCHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHC
GVKVTLRIANDYAAVIEGQRAGNIHIASYGSASFARARLTGVKTDAFANDINADGSTGYY
CEEEEEEECCCEEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCHHHHCCCCCCCCCCEE
SVFFVKANSPYKKVEDLKGKNLGLVDPNSTSGNNVPRFELNKMGVTDAEAYFGKVVFTGS
EEEEEECCCCHHHHHHCCCCCCEEECCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEECC
HENAVLALSQGTVDVAANQWTSDNDSTLAQMLTKGMLKNADGSPMKKDDFRIIHKSAPII
CCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEE
NGPYAYNTDLPEDAKQAIAKAFFDAPTKDKAAFDRLSDGQKKGFHPATTKDWDGTIDLIK
CCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
FVDSLRKKAS
HHHHHHHHCC
>Mature Secondary Structure
MINRRIVLAGVAALAFTAQASAEDWKAKYPELTFAVVPAENASGVTERWTPFVNYLSKEL
CCCCEEEEHHHHHHHHHHCCCCHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHC
GVKVTLRIANDYAAVIEGQRAGNIHIASYGSASFARARLTGVKTDAFANDINADGSTGYY
CEEEEEEECCCEEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCHHHHCCCCCCCCCCEE
SVFFVKANSPYKKVEDLKGKNLGLVDPNSTSGNNVPRFELNKMGVTDAEAYFGKVVFTGS
EEEEEECCCCHHHHHHCCCCCCEEECCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEECC
HENAVLALSQGTVDVAANQWTSDNDSTLAQMLTKGMLKNADGSPMKKDDFRIIHKSAPII
CCCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEE
NGPYAYNTDLPEDAKQAIAKAFFDAPTKDKAAFDRLSDGQKKGFHPATTKDWDGTIDLIK
CCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
FVDSLRKKAS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]