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The map label for this gene is phnE [H]

Identifier: 146342211

GI number: 146342211

Start: 5569902

End: 5570750

Strand: Reverse

Name: phnE [H]

Synonym: BRADO5363

Alternate gene names: 146342211

Gene position: 5570750-5569902 (Counterclockwise)

Preceding gene: 146342212

Following gene: 146342210

Centisome position: 74.71

GC content: 67.37

Gene sequence:

>849_bases
ATGAGCCTTCCGGACACGCCGGATCCGACGCAGCTGCGCGAGCGCTATCCGCACATCTTCAACCGGCCGATGTCGGCACG
GCTCGCGACCCCCTTGGTGCTGCTGCTGGCGCTCGCCGTCTTCGTCTTCGGTCTCGTCGACCTGGATTTCTCGCCGTCGC
GCATTCTGTCCGGCCTTGGCCAGCTCGGCTGGATCGCGCGTTTGATGCTGCCGCCCGACCCGGGCGGCTCGCTGCCGGTC
TATCTCAAGGCGCTCGGCGAGACGCTGTCGATCGCGGTGCTCGGCACCACGCTCGCCGCATGCCTTGCGTTCCCGGTCAG
CCTGCTCGCCGCCCGCAACGTCATTCCCTCGGCGATCTTCCGCTTCCCGGTGCGGCGGCTGTTCGACACGATCCGCGGCG
TCGACACGCTGATCTGGGCGCTGGTCTGGATCAATGTCGTCGGGCTCGGCCCCTTCGCTGGCGTGCTCGCGATCGCGGTC
TCGGACTTCGGCGCGCTCGGCAAGCTGTTCTCGGAGGCGATGGAGAGCGCCGACCGCAAGCAGGTCGAAGGCGTGCGCGC
CGCCGGCGGCAACGCGCTGCATGAGATCCGATTCGGCCTGCTGCCGCAGGTGCTTCCGGTCATCGCGGGGCAGGTGCTGT
ACTTCATCGAGTCCAACACCCGTTCGGCGACCATCATCGGCATCGTTGGTGCCGGCGGCATCGGCCTGCAGCTCGCCGAA
CAGATTCGCGTGCTGGAATGGCAGAAGGTGTCGTTCCTGATCCTGATGATTCTGGTGGCGGTGGCCGCGATCGACTGGAT
CTCGAACAGGCTGCGCTTCGCCATCATCGGACGTCGCGCCGTCGCCTGA

Upstream 100 bases:

>100_bases
ACGTCAGCGCCATCCTCGTGATGATCGTGGTGACGGTGTTCCTGATCGATATCGGCACCGGCTGGCTGCGCGGGCGGCTG
TTCGGCGCGGAGGCGCGGAC

Downstream 100 bases:

>100_bases
TCGCGGCGGCATCTGAAGGTTGCGATTGTTCGTAGGCGAACGATCCAGCTGTGCTCGCGCGTGTGTGCACTTCACGCGCT
GTCGCAAATCTTTCATCTGC

Product: putative PhnE protein, phosphonate ABC transporter membrane protein

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSLPDTPDPTQLRERYPHIFNRPMSARLATPLVLLLALAVFVFGLVDLDFSPSRILSGLGQLGWIARLMLPPDPGGSLPV
YLKALGETLSIAVLGTTLAACLAFPVSLLAARNVIPSAIFRFPVRRLFDTIRGVDTLIWALVWINVVGLGPFAGVLAIAV
SDFGALGKLFSEAMESADRKQVEGVRAAGGNALHEIRFGLLPQVLPVIAGQVLYFIESNTRSATIIGIVGAGGIGLQLAE
QIRVLEWQKVSFLILMILVAVAAIDWISNRLRFAIIGRRAVA

Sequences:

>Translated_282_residues
MSLPDTPDPTQLRERYPHIFNRPMSARLATPLVLLLALAVFVFGLVDLDFSPSRILSGLGQLGWIARLMLPPDPGGSLPV
YLKALGETLSIAVLGTTLAACLAFPVSLLAARNVIPSAIFRFPVRRLFDTIRGVDTLIWALVWINVVGLGPFAGVLAIAV
SDFGALGKLFSEAMESADRKQVEGVRAAGGNALHEIRFGLLPQVLPVIAGQVLYFIESNTRSATIIGIVGAGGIGLQLAE
QIRVLEWQKVSFLILMILVAVAAIDWISNRLRFAIIGRRAVA
>Mature_281_residues
SLPDTPDPTQLRERYPHIFNRPMSARLATPLVLLLALAVFVFGLVDLDFSPSRILSGLGQLGWIARLMLPPDPGGSLPVY
LKALGETLSIAVLGTTLAACLAFPVSLLAARNVIPSAIFRFPVRRLFDTIRGVDTLIWALVWINVVGLGPFAGVLAIAVS
DFGALGKLFSEAMESADRKQVEGVRAAGGNALHEIRFGLLPQVLPVIAGQVLYFIESNTRSATIIGIVGAGGIGLQLAEQ
IRVLEWQKVSFLILMILVAVAAIDWISNRLRFAIIGRRAVA

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30325; Mature: 30194

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPDTPDPTQLRERYPHIFNRPMSARLATPLVLLLALAVFVFGLVDLDFSPSRILSGLG
CCCCCCCCHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QLGWIARLMLPPDPGGSLPVYLKALGETLSIAVLGTTLAACLAFPVSLLAARNVIPSAIF
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFPVRRLFDTIRGVDTLIWALVWINVVGLGPFAGVLAIAVSDFGALGKLFSEAMESADRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVEGVRAAGGNALHEIRFGLLPQVLPVIAGQVLYFIESNTRSATIIGIVGAGGIGLQLAE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCCHHHHHHH
QIRVLEWQKVSFLILMILVAVAAIDWISNRLRFAIIGRRAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHCC
>Mature Secondary Structure 
SLPDTPDPTQLRERYPHIFNRPMSARLATPLVLLLALAVFVFGLVDLDFSPSRILSGLG
CCCCCCCHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QLGWIARLMLPPDPGGSLPVYLKALGETLSIAVLGTTLAACLAFPVSLLAARNVIPSAIF
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFPVRRLFDTIRGVDTLIWALVWINVVGLGPFAGVLAIAVSDFGALGKLFSEAMESADRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVEGVRAAGGNALHEIRFGLLPQVLPVIAGQVLYFIESNTRSATIIGIVGAGGIGLQLAE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCCHHHHHHH
QIRVLEWQKVSFLILMILVAVAAIDWISNRLRFAIIGRRAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]