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The map label for this gene is metK [H]

Identifier: 146342096

GI number: 146342096

Start: 5451792

End: 5452991

Strand: Reverse

Name: metK [H]

Synonym: BRADO5239

Alternate gene names: 146342096

Gene position: 5452991-5451792 (Counterclockwise)

Preceding gene: 146342097

Following gene: 146342095

Centisome position: 73.13

GC content: 66.67

Gene sequence:

>1200_bases
TTGATGCGCGCGTCATATCTGTTCACCTCGGAATCGGTGTCCGAGGGCCATCCGGACAAGGTCTGTGACCGGATCTCGGA
CGAGATCGTCGACCTGTTCTACCGTGAAGGGCCGAAGGCCGGCATCGACCCCTGGCAGATCCGCGCCGCCTGCGAGACGC
TCGCCACCACCAACAAGGTCGTGATCGCCGGCGAGACCCGCGGCCCGTCCTCGGTGACGAATGAGCACATCGAGCACGTC
GTCCGCGAGGCGATCAAGGATATCGGCTATGAGCAGGAGGGCTTCCACTGGAAGACCTGCGACATCGAGATCCTCCTGCA
TCCGCAGTCGGCCGACATCGCGCAGGGCGTCGACGCGCTGCAGCCGGGCGAAGTCAAGGAAGAGGGCGCCGGCGACCAGG
GCATCATGTTCGGCTACGCCTGCAACGAGACGCCCGACCTCATGCCGGCGCCGATCTTCTATGCCCACAAGATCCTGCGC
ATGATCGCCGAAGCCCGGCATTCCGGCGTCGAGAAGGTGCTGGGCCCGGATTCCAAGAGCCAGGTCACCGTGCGCTATGA
GAATGGCAAGCCGGTCGGCGTGCGCGAGATCGTCGTCTCGCACCAGCACCTGATCCCGGATATGACCTCGGCGCAGGTGC
GCGAGCGGGTCGAGCCCTATGTGCGCGAGGCGCTGCCGAAGGACTGGATCACCAAGGACACGATCTGGCACATCAACCCG
ACCGGCAAGTTCTACATCGGCGGCCCCGATGGCGACACCGGCCTCACGGGCCGCAAGATCATCGTCGACACGTATGGTGG
CGCGGCCCCGCATGGCGGCGGCGCGTTCTCCGGCAAGGACCCGACCAAGGTCGACCGCTCGGCCGCCTATGCGGCGCGCT
ACCTCGCCAAGAACATCGTGGCCGCCGGCCTCGCCGATCGCTGCACGCTGCAGCTCGCCTACGCCATCGGCGTGGCGCGG
CCGCTGTCGATCTACATCGACACCCACGGCACCGGTAAGGTCTCCGAGGACAAGCTCGAGAAGGCGGCGGCCGAGGTGAT
GAACCTCACCCCGCGCGGCATCCGCTCGCATCTCGACCTCAACCGTCCGATCTACGCGCGCACCGCGGCGTATGGCCATT
TCGGCCGCACGCCCGACAACGAGGGCGGCTTCTCCTGGGAGAAGACCGACCTCGCCGAGGCGTTCAAGCGCGCGGTGTAG

Upstream 100 bases:

>100_bases
CGAGTCCTACGTCATCTCGCGGCGTCGCGACTGACATCCTGGGATCAGGACATTTGGGATCAATACGAACGACCGCCGTG
GGCGGCAAAGAAGGGATTGC

Downstream 100 bases:

>100_bases
TTGCTACTTCACCTCTCCCCGCCAGGGCGAAGCGAAGCTTCGCGGGGGCGGGGAAAGGTCGGATTGCACCGAAGATGCAA
TCCGGGTGAGGGGACTCTCC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MMRASYLFTSESVSEGHPDKVCDRISDEIVDLFYREGPKAGIDPWQIRAACETLATTNKVVIAGETRGPSSVTNEHIEHV
VREAIKDIGYEQEGFHWKTCDIEILLHPQSADIAQGVDALQPGEVKEEGAGDQGIMFGYACNETPDLMPAPIFYAHKILR
MIAEARHSGVEKVLGPDSKSQVTVRYENGKPVGVREIVVSHQHLIPDMTSAQVRERVEPYVREALPKDWITKDTIWHINP
TGKFYIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNIVAAGLADRCTLQLAYAIGVAR
PLSIYIDTHGTGKVSEDKLEKAAAEVMNLTPRGIRSHLDLNRPIYARTAAYGHFGRTPDNEGGFSWEKTDLAEAFKRAV

Sequences:

>Translated_399_residues
MMRASYLFTSESVSEGHPDKVCDRISDEIVDLFYREGPKAGIDPWQIRAACETLATTNKVVIAGETRGPSSVTNEHIEHV
VREAIKDIGYEQEGFHWKTCDIEILLHPQSADIAQGVDALQPGEVKEEGAGDQGIMFGYACNETPDLMPAPIFYAHKILR
MIAEARHSGVEKVLGPDSKSQVTVRYENGKPVGVREIVVSHQHLIPDMTSAQVRERVEPYVREALPKDWITKDTIWHINP
TGKFYIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNIVAAGLADRCTLQLAYAIGVAR
PLSIYIDTHGTGKVSEDKLEKAAAEVMNLTPRGIRSHLDLNRPIYARTAAYGHFGRTPDNEGGFSWEKTDLAEAFKRAV
>Mature_399_residues
MMRASYLFTSESVSEGHPDKVCDRISDEIVDLFYREGPKAGIDPWQIRAACETLATTNKVVIAGETRGPSSVTNEHIEHV
VREAIKDIGYEQEGFHWKTCDIEILLHPQSADIAQGVDALQPGEVKEEGAGDQGIMFGYACNETPDLMPAPIFYAHKILR
MIAEARHSGVEKVLGPDSKSQVTVRYENGKPVGVREIVVSHQHLIPDMTSAQVRERVEPYVREALPKDWITKDTIWHINP
TGKFYIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNIVAAGLADRCTLQLAYAIGVAR
PLSIYIDTHGTGKVSEDKLEKAAAEVMNLTPRGIRSHLDLNRPIYARTAAYGHFGRTPDNEGGFSWEKTDLAEAFKRAV

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=392, Percent_Identity=48.9795918367347, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI4557737, Length=389, Percent_Identity=48.8431876606684, Blast_Score=327, Evalue=1e-89,
Organism=Escherichia coli, GI1789311, Length=398, Percent_Identity=52.7638190954774, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17538498, Length=395, Percent_Identity=47.5949367088608, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17538494, Length=395, Percent_Identity=47.5949367088608, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI25145633, Length=395, Percent_Identity=46.8354430379747, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17551082, Length=394, Percent_Identity=46.1928934010152, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI32565905, Length=393, Percent_Identity=44.2748091603053, Blast_Score=283, Evalue=9e-77,
Organism=Caenorhabditis elegans, GI32565911, Length=316, Percent_Identity=49.6835443037975, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI25145635, Length=316, Percent_Identity=48.7341772151899, Blast_Score=268, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI32565903, Length=302, Percent_Identity=49.3377483443709, Blast_Score=255, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI32565909, Length=91, Percent_Identity=41.7582417582418, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=391, Percent_Identity=46.5473145780051, Blast_Score=320, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6323209, Length=394, Percent_Identity=45.6852791878173, Blast_Score=316, Evalue=4e-87,
Organism=Drosophila melanogaster, GI45552159, Length=391, Percent_Identity=49.1048593350384, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24580529, Length=391, Percent_Identity=49.1048593350384, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24580537, Length=391, Percent_Identity=49.1048593350384, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24580533, Length=391, Percent_Identity=49.1048593350384, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24580535, Length=391, Percent_Identity=49.1048593350384, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24580531, Length=391, Percent_Identity=49.1048593350384, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24580527, Length=391, Percent_Identity=48.5933503836317, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24580525, Length=391, Percent_Identity=48.5933503836317, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24580541, Length=390, Percent_Identity=46.4102564102564, Blast_Score=287, Evalue=9e-78,
Organism=Drosophila melanogaster, GI24580539, Length=132, Percent_Identity=42.4242424242424, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43683; Mature: 43683

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRASYLFTSESVSEGHPDKVCDRISDEIVDLFYREGPKAGIDPWQIRAACETLATTNKV
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEE
VIAGETRGPSSVTNEHIEHVVREAIKDIGYEQEGFHWKTCDIEILLHPQSADIAQGVDAL
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCHHHHCCCCC
QPGEVKEEGAGDQGIMFGYACNETPDLMPAPIFYAHKILRMIAEARHSGVEKVLGPDSKS
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCC
QVTVRYENGKPVGVREIVVSHQHLIPDMTSAQVRERVEPYVREALPKDWITKDTIWHINP
EEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC
TGKFYIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNIV
CCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AAGLADRCTLQLAYAIGVARPLSIYIDTHGTGKVSEDKLEKAAAEVMNLTPRGIRSHLDL
HHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCC
NRPIYARTAAYGHFGRTPDNEGGFSWEKTDLAEAFKRAV
CCCEEEHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MMRASYLFTSESVSEGHPDKVCDRISDEIVDLFYREGPKAGIDPWQIRAACETLATTNKV
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEE
VIAGETRGPSSVTNEHIEHVVREAIKDIGYEQEGFHWKTCDIEILLHPQSADIAQGVDAL
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCHHHHCCCCC
QPGEVKEEGAGDQGIMFGYACNETPDLMPAPIFYAHKILRMIAEARHSGVEKVLGPDSKS
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCC
QVTVRYENGKPVGVREIVVSHQHLIPDMTSAQVRERVEPYVREALPKDWITKDTIWHINP
EEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC
TGKFYIGGPDGDTGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYAARYLAKNIV
CCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AAGLADRCTLQLAYAIGVARPLSIYIDTHGTGKVSEDKLEKAAAEVMNLTPRGIRSHLDL
HHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCC
NRPIYARTAAYGHFGRTPDNEGGFSWEKTDLAEAFKRAV
CCCEEEHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA