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The map label for this gene is livK [C]

Identifier: 146342001

GI number: 146342001

Start: 5336758

End: 5337918

Strand: Direct

Name: livK [C]

Synonym: BRADO5137

Alternate gene names: 146342001

Gene position: 5336758-5337918 (Clockwise)

Preceding gene: 146341999

Following gene: 146342009

Centisome position: 71.57

GC content: 66.15

Gene sequence:

>1161_bases
ATGAACTGGTTCTCAAGCTTCCGGACATCCCTTGTCCTCATCCTGCTCGCGGGACTGCTTCCGCTCGGGTCTGCGCGCGC
CGACGAGCTCGGCGTCACCAATGATTCGATCCTGTTCGGGCAGGTTGCGGCGCTGGAGGGTCCGTCGGCTGCGCTCGGTC
AGGCGGCGCGCCAGGGGCTGCTGGCGGCGTTCAGCGAGCTCAACGCCAAGGGCGGCGTCTATGGCCGTCGGCTGAAGCTC
GCGAGCCGCAACGACGGCTACGATCCCGACCGCTCCGTGGTCGAGACCATCAAGCTGATCTACGAGGACAAGGTGTTCGC
ATTGATCGGCGCCGTCGGCACGCCGACCGCGATGGCCACCGCGCCGATCGCGGCCTCGAACGACGTGCCGTTCATCGGTC
CGGTGTCCGGCGCCGAATTCCTGCGCACGCCTGATTTTCAGAACATCGTCAACATTCGCGCGAGCTACGCGGCCGAGGCG
GAAGCTTCGATCAAGCACCTCACCGAAGACCTGCGTCTGACGCGCATCGCCATCTTCTATCAGGATGACGCGTTTGGCCG
CGACGTGCTGGCGGGCGTGAAGACCCATCTCGACAAACGCGGCCTGGAGCTGGCCGCCGAAGGCACCTTCGAGCGCAATA
CGCGCGCCGTCGGCGCGGCGCTCAAGGTGATCCGCCGTGCCGAGCCGGAGGCCGTCATCCTCGTCGGCACCTACGGGCCA
TGCGCCGAGTTCATCAAGATGGCGCACCGGAGCGGCTTCAACCCGACCTTCTCGGCCGTGTCCTTCGTCGGCGGCAACGC
GCTCGCCAAGGAGCTCGGCTCGGAAGGGCGTGGCGTCATCGTCTCCGAGGTGGTGCCCTTCCCGTGGGACACCGATCGCC
GGATCGTCGCCGACTATCAGGCGGCGATGAAGGAGCTCGATCCGAGCCAGACACCCGACTTCATCGGGCTCGAAGGCTAC
ATTACCGGGCGTCTTGTGGCGCGCGCGCTGGCGATGACCGGGCCCAATCCGACCCGCGCCGAGCTGCTGCGGATCATCAA
CGATGTGGGACAGTTCGACATCGGTGGTCTCGTCGTCAGCTTCGGCAGCGCACGCAAGGATAATCCGCCGCCGGTGTCGC
TGGTGGTGATTCAGAGCGACGGCAGCTTCAAGCCGATCTGA

Upstream 100 bases:

>100_bases
CGTAAAACGCGATCAAGCCGGCGGTACGTCTTTAGCGAGGTCTTCATTCAACGCGTGTAGGCTCGATCTCATCAAGCGCA
CCCGTGCATCGAAGATCGCA

Downstream 100 bases:

>100_bases
CCACGCCTCAGCTCGCAGGTTTAGCTCGAAGGCTCGCTCGAAGATTTAGCTAAGATTTCTTGGGCTTCGGCGGGCCTTCG
ACCGGCGGCAGCGATTTCAG

Product: putative extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MNWFSSFRTSLVLILLAGLLPLGSARADELGVTNDSILFGQVAALEGPSAALGQAARQGLLAAFSELNAKGGVYGRRLKL
ASRNDGYDPDRSVVETIKLIYEDKVFALIGAVGTPTAMATAPIAASNDVPFIGPVSGAEFLRTPDFQNIVNIRASYAAEA
EASIKHLTEDLRLTRIAIFYQDDAFGRDVLAGVKTHLDKRGLELAAEGTFERNTRAVGAALKVIRRAEPEAVILVGTYGP
CAEFIKMAHRSGFNPTFSAVSFVGGNALAKELGSEGRGVIVSEVVPFPWDTDRRIVADYQAAMKELDPSQTPDFIGLEGY
ITGRLVARALAMTGPNPTRAELLRIINDVGQFDIGGLVVSFGSARKDNPPPVSLVVIQSDGSFKPI

Sequences:

>Translated_386_residues
MNWFSSFRTSLVLILLAGLLPLGSARADELGVTNDSILFGQVAALEGPSAALGQAARQGLLAAFSELNAKGGVYGRRLKL
ASRNDGYDPDRSVVETIKLIYEDKVFALIGAVGTPTAMATAPIAASNDVPFIGPVSGAEFLRTPDFQNIVNIRASYAAEA
EASIKHLTEDLRLTRIAIFYQDDAFGRDVLAGVKTHLDKRGLELAAEGTFERNTRAVGAALKVIRRAEPEAVILVGTYGP
CAEFIKMAHRSGFNPTFSAVSFVGGNALAKELGSEGRGVIVSEVVPFPWDTDRRIVADYQAAMKELDPSQTPDFIGLEGY
ITGRLVARALAMTGPNPTRAELLRIINDVGQFDIGGLVVSFGSARKDNPPPVSLVVIQSDGSFKPI
>Mature_386_residues
MNWFSSFRTSLVLILLAGLLPLGSARADELGVTNDSILFGQVAALEGPSAALGQAARQGLLAAFSELNAKGGVYGRRLKL
ASRNDGYDPDRSVVETIKLIYEDKVFALIGAVGTPTAMATAPIAASNDVPFIGPVSGAEFLRTPDFQNIVNIRASYAAEA
EASIKHLTEDLRLTRIAIFYQDDAFGRDVLAGVKTHLDKRGLELAAEGTFERNTRAVGAALKVIRRAEPEAVILVGTYGP
CAEFIKMAHRSGFNPTFSAVSFVGGNALAKELGSEGRGVIVSEVVPFPWDTDRRIVADYQAAMKELDPSQTPDFIGLEGY
ITGRLVARALAMTGPNPTRAELLRIINDVGQFDIGGLVVSFGSARKDNPPPVSLVVIQSDGSFKPI

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=335, Percent_Identity=21.7910447761194, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI48994941, Length=301, Percent_Identity=23.9202657807309, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41150; Mature: 41150

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWFSSFRTSLVLILLAGLLPLGSARADELGVTNDSILFGQVAALEGPSAALGQAARQGL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHCCCCCHHHHHHHHHHH
LAAFSELNAKGGVYGRRLKLASRNDGYDPDRSVVETIKLIYEDKVFALIGAVGTPTAMAT
HHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHC
APIAASNDVPFIGPVSGAEFLRTPDFQNIVNIRASYAAEAEASIKHLTEDLRLTRIAIFY
CCCCCCCCCCEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEE
QDDAFGRDVLAGVKTHLDKRGLELAAEGTFERNTRAVGAALKVIRRAEPEAVILVGTYGP
ECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCH
CAEFIKMAHRSGFNPTFSAVSFVGGNALAKELGSEGRGVIVSEVVPFPWDTDRRIVADYQ
HHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHH
AAMKELDPSQTPDFIGLEGYITGRLVARALAMTGPNPTRAELLRIINDVGQFDIGGLVVS
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHEEE
FGSARKDNPPPVSLVVIQSDGSFKPI
CCCCCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MNWFSSFRTSLVLILLAGLLPLGSARADELGVTNDSILFGQVAALEGPSAALGQAARQGL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHCCCCCHHHHHHHHHHH
LAAFSELNAKGGVYGRRLKLASRNDGYDPDRSVVETIKLIYEDKVFALIGAVGTPTAMAT
HHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHC
APIAASNDVPFIGPVSGAEFLRTPDFQNIVNIRASYAAEAEASIKHLTEDLRLTRIAIFY
CCCCCCCCCCEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEE
QDDAFGRDVLAGVKTHLDKRGLELAAEGTFERNTRAVGAALKVIRRAEPEAVILVGTYGP
ECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCH
CAEFIKMAHRSGFNPTFSAVSFVGGNALAKELGSEGRGVIVSEVVPFPWDTDRRIVADYQ
HHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHH
AAMKELDPSQTPDFIGLEGYITGRLVARALAMTGPNPTRAELLRIINDVGQFDIGGLVVS
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHEEE
FGSARKDNPPPVSLVVIQSDGSFKPI
CCCCCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA