Click here to switch to the map view.

The map label for this gene is glgX [H]

Identifier: 146341960

GI number: 146341960

Start: 5296083

End: 5298317

Strand: Reverse

Name: glgX [H]

Synonym: BRADO5096

Alternate gene names: 146341960

Gene position: 5298317-5296083 (Counterclockwise)

Preceding gene: 146341961

Following gene: 146341958

Centisome position: 71.06

GC content: 64.12

Gene sequence:

>2235_bases
ATGGATCAACTCACTGACAGGGCCGATGCCCTGACGCAGGCGGCTTTCAGTCTGCGCAAGGCCAAGATCAGCGAAGGCCG
GCCGTTTCCGCTCGGCGCCACCTGGGACGGGCTCGGCGTCAATTTCGCGATCTTTTCCGCGCACGCCACCAAGGTCGAGC
TGTGTTTGTTCGACGAGACCGGCGAGACCGAGCTCGAGCGCATCGAACTGCCGGAATATACCGACGAGGTCTGGCACGGC
TATCTGCCGACCGCGCGTCCGGGCACCGTCTATGGCTATCGCGTGCATGGCCCCTACGAGCCCGATGCCGGCCATCGCTT
CAATCCGAACAAGCTCGTCATCGACCCCTACGCCAAGCAGCTCGTCGGCCAGCTGCGCTGGGGTCCCGAGCTGTTCGGCT
ATCAGCTGGATCACGCCGACAAGGATCTCTCCTTCGACGACCGCGACAGCGCGCCCCTGATGCTGAAGTGCCGGGTGATC
GATCCCGCCTTCACCTGGGGCACCGCCCGCAAGCCGGAGATCCCGTGGGAGCGGACCATCTTCTATGAGATGCACGTCAA
GGGCTTCACCAAGCTGCATCCGCTGGTGCCGGAGGCCGACCGCGGCACTTTCGCCGGCCTCGCGCATCAGGATATTCCGG
CCTACCTGCGCTCGCTCGGCATCACCTCGGCCGAGCTGCTGCCGATCCATGCCTTCATCGATGACAGCTATCTGGTCGAC
AAGGGCCTGCGCAACTATTGGGGCTACAATTCGATCGGCTTCTTCGCGCCCGAGCCGCGCTACCTCAAGACGCCGCTCGC
GACCGAATTCAAGACCATGGTCAACCAGTTCCACGCAAACGGCATCGAGGTGATCCTCGACGTGGTCTATAACCACACCG
CCGAGGGCAACGAGCTCGGCCCGACCCTGTCTTTCAAGGGCATCGACAACGCCAGCTACTACCGGCTGCTGCCGGACAAG
AAGCGCTATTACATCAACGATACCGGCACCGGTAACACTGTGAACCTGTCGCATCAGCGCGTGCTCCAGCTGGTCGCTGA
CTCCTTGCGCTATTGGGCGACGGAGATGCGCGTCGACGGTTTCCGTTTCGATCTCGCCACCATTCTCGCGCGCGAGCCCT
ATGGCTTCGACGAGGGCGGCGGCTTTCTCGATGCCTGCCGCCAGGATCCGGTGCTGTCGGGCGTCAAGCTGATCGCAGAG
CCCTGGGACATCGGTCCGGGCGGCTATCAGGTCGGTCAGTTTCCGCCGGGCTGGGCGGAGTGGAACGACAAGTTTCGCGA
CACCACGCGCGCGTTCTGGAAGGGCGACGGCGGCACCCTCGCCGATTTCGCCAAGCGCATCTCCGGCTCCGGTGATCTTT
TCAACAAGCGCGGTCGCCGGCCGTGGGCGAGCGTCAATTTCATCACCGCCCATGACGGCTTCAACCTCAACGACCTCGTC
TCCTATAACGATAAGCATAATGAGGCGAATGGCGAGGACAATCGCGACGGCCACAGCAACAATCATTCGTGGAATTGCGG
CGTCGAAGGGCCGACCGACGATGCGGAGATCATCGCGTTGCGCGAACGGCAGAAGCGCAACATGCTGGCGACGATGCTGC
TGTCGCACGGCACGCCGATGCTGCTCGCCGGCGACGAGTTCGGCCACACCCAGGACGGCAACAACAACGCCTATGCCCAG
GACAATGAGATCAGCTGGCTCGACTGGATGGGGATATCAGCGCCCGGGCGGCAGCTGCGCGAGTTCACGCGCAAGCTGAT
CGCGATGCGCAAGGCGTTCCCGATCCTGTATCGCTCGCGCTTCCTGGTCGGCTCGCTCAACGAGGAGCTCGACGTCAAGG
ACGTGACCTGGCTGGCGCCATCAGGCGAGGAGATGGCGACGGAGCAGTGGACCGACGGCAACGCACGCTGCTTCGGCATG
CTGCTCGACGGCCGCGCCCAGGAGACCGGCGTCAAGCGGCGCGGCTCGGATGCGACGCTGCTCTTGATCTACAACGCGCA
TTTCGACGTCGTGAACTTCACTTTGCCGTCGGTGCCCGAGGGGCACAATTGGCTGGCGCTGATCGATACCAATCAGCCGG
ACGCCCAGGTGCAGTCGCTGCCGTTCGGCCATGTCTACGAGGTCACCGGCCGCTCCATGCTGGCGCTCGGCCTGTCCTCG
GAGCAGCAGCCGATGCGCGGTCTCCGCCACGGCCTCGGCGCCCTGCTCGACATCGCGGAGACGCCGCTGGGGTAG

Upstream 100 bases:

>100_bases
ATCGCCTCCGTTACGGCGACCGAGACGGTCGTCGCTGATGCCGTCGCAACCACCGAAACACGTTAGTTCAGTCGGCTCAG
AAGCCGGGCAGGGGGCATTG

Downstream 100 bases:

>100_bases
GGGTGTGGGGGGAGAGCTTGTTAAACGCGTCGGGGCAGGGGAGCTCGTGGCCGTCCTTCGAGGCATGCGCCGAGTCGGAG
CCGCTCCCGCGTAGCCCGGA

Product: glycosyl hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 744; Mature: 744

Protein sequence:

>744_residues
MDQLTDRADALTQAAFSLRKAKISEGRPFPLGATWDGLGVNFAIFSAHATKVELCLFDETGETELERIELPEYTDEVWHG
YLPTARPGTVYGYRVHGPYEPDAGHRFNPNKLVIDPYAKQLVGQLRWGPELFGYQLDHADKDLSFDDRDSAPLMLKCRVI
DPAFTWGTARKPEIPWERTIFYEMHVKGFTKLHPLVPEADRGTFAGLAHQDIPAYLRSLGITSAELLPIHAFIDDSYLVD
KGLRNYWGYNSIGFFAPEPRYLKTPLATEFKTMVNQFHANGIEVILDVVYNHTAEGNELGPTLSFKGIDNASYYRLLPDK
KRYYINDTGTGNTVNLSHQRVLQLVADSLRYWATEMRVDGFRFDLATILAREPYGFDEGGGFLDACRQDPVLSGVKLIAE
PWDIGPGGYQVGQFPPGWAEWNDKFRDTTRAFWKGDGGTLADFAKRISGSGDLFNKRGRRPWASVNFITAHDGFNLNDLV
SYNDKHNEANGEDNRDGHSNNHSWNCGVEGPTDDAEIIALRERQKRNMLATMLLSHGTPMLLAGDEFGHTQDGNNNAYAQ
DNEISWLDWMGISAPGRQLREFTRKLIAMRKAFPILYRSRFLVGSLNEELDVKDVTWLAPSGEEMATEQWTDGNARCFGM
LLDGRAQETGVKRRGSDATLLLIYNAHFDVVNFTLPSVPEGHNWLALIDTNQPDAQVQSLPFGHVYEVTGRSMLALGLSS
EQQPMRGLRHGLGALLDIAETPLG

Sequences:

>Translated_744_residues
MDQLTDRADALTQAAFSLRKAKISEGRPFPLGATWDGLGVNFAIFSAHATKVELCLFDETGETELERIELPEYTDEVWHG
YLPTARPGTVYGYRVHGPYEPDAGHRFNPNKLVIDPYAKQLVGQLRWGPELFGYQLDHADKDLSFDDRDSAPLMLKCRVI
DPAFTWGTARKPEIPWERTIFYEMHVKGFTKLHPLVPEADRGTFAGLAHQDIPAYLRSLGITSAELLPIHAFIDDSYLVD
KGLRNYWGYNSIGFFAPEPRYLKTPLATEFKTMVNQFHANGIEVILDVVYNHTAEGNELGPTLSFKGIDNASYYRLLPDK
KRYYINDTGTGNTVNLSHQRVLQLVADSLRYWATEMRVDGFRFDLATILAREPYGFDEGGGFLDACRQDPVLSGVKLIAE
PWDIGPGGYQVGQFPPGWAEWNDKFRDTTRAFWKGDGGTLADFAKRISGSGDLFNKRGRRPWASVNFITAHDGFNLNDLV
SYNDKHNEANGEDNRDGHSNNHSWNCGVEGPTDDAEIIALRERQKRNMLATMLLSHGTPMLLAGDEFGHTQDGNNNAYAQ
DNEISWLDWMGISAPGRQLREFTRKLIAMRKAFPILYRSRFLVGSLNEELDVKDVTWLAPSGEEMATEQWTDGNARCFGM
LLDGRAQETGVKRRGSDATLLLIYNAHFDVVNFTLPSVPEGHNWLALIDTNQPDAQVQSLPFGHVYEVTGRSMLALGLSS
EQQPMRGLRHGLGALLDIAETPLG
>Mature_744_residues
MDQLTDRADALTQAAFSLRKAKISEGRPFPLGATWDGLGVNFAIFSAHATKVELCLFDETGETELERIELPEYTDEVWHG
YLPTARPGTVYGYRVHGPYEPDAGHRFNPNKLVIDPYAKQLVGQLRWGPELFGYQLDHADKDLSFDDRDSAPLMLKCRVI
DPAFTWGTARKPEIPWERTIFYEMHVKGFTKLHPLVPEADRGTFAGLAHQDIPAYLRSLGITSAELLPIHAFIDDSYLVD
KGLRNYWGYNSIGFFAPEPRYLKTPLATEFKTMVNQFHANGIEVILDVVYNHTAEGNELGPTLSFKGIDNASYYRLLPDK
KRYYINDTGTGNTVNLSHQRVLQLVADSLRYWATEMRVDGFRFDLATILAREPYGFDEGGGFLDACRQDPVLSGVKLIAE
PWDIGPGGYQVGQFPPGWAEWNDKFRDTTRAFWKGDGGTLADFAKRISGSGDLFNKRGRRPWASVNFITAHDGFNLNDLV
SYNDKHNEANGEDNRDGHSNNHSWNCGVEGPTDDAEIIALRERQKRNMLATMLLSHGTPMLLAGDEFGHTQDGNNNAYAQ
DNEISWLDWMGISAPGRQLREFTRKLIAMRKAFPILYRSRFLVGSLNEELDVKDVTWLAPSGEEMATEQWTDGNARCFGM
LLDGRAQETGVKRRGSDATLLLIYNAHFDVVNFTLPSVPEGHNWLALIDTNQPDAQVQSLPFGHVYEVTGRSMLALGLSS
EQQPMRGLRHGLGALLDIAETPLG

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=658, Percent_Identity=47.112462006079, Blast_Score=570, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 83476; Mature: 83476

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQLTDRADALTQAAFSLRKAKISEGRPFPLGATWDGLGVNFAIFSAHATKVELCLFDET
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCC
GETELERIELPEYTDEVWHGYLPTARPGTVYGYRVHGPYEPDAGHRFNPNKLVIDPYAKQ
CCCCHHEECCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECHHHHH
LVGQLRWGPELFGYQLDHADKDLSFDDRDSAPLMLKCRVIDPAFTWGTARKPEIPWERTI
HHHHHCCCHHHHCEEECCCCCCCCCCCCCCCCEEEEEEEECCHHCCCCCCCCCCCCCEEE
FYEMHVKGFTKLHPLVPEADRGTFAGLAHQDIPAYLRSLGITSAELLPIHAFIDDSYLVD
EEEEECCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHEEEEEEEECCCHHHH
KGLRNYWGYNSIGFFAPEPRYLKTPLATEFKTMVNQFHANGIEVILDVVYNHTAEGNELG
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
PTLSFKGIDNASYYRLLPDKKRYYINDTGTGNTVNLSHQRVLQLVADSLRYWATEMRVDG
CEEEECCCCCCCEEEECCCCCEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCC
FRFDLATILAREPYGFDEGGGFLDACRQDPVLSGVKLIAEPWDIGPGGYQVGQFPPGWAE
EEEHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHCHHHEECCCCCCCCCCCCCCCCCCHHH
WNDKFRDTTRAFWKGDGGTLADFAKRISGSGDLFNKRGRRPWASVNFITAHDGFNLNDLV
HCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCEEEEEEECCCCCHHHHC
SYNDKHNEANGEDNRDGHSNNHSWNCGVEGPTDDAEIIALRERQKRNMLATMLLSHGTPM
CCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHEEEEHHHHHHHHHHHHHHHCCCCE
LLAGDEFGHTQDGNNNAYAQDNEISWLDWMGISAPGRQLREFTRKLIAMRKAFPILYRSR
EEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
FLVGSLNEELDVKDVTWLAPSGEEMATEQWTDGNARCFGMLLDGRAQETGVKRRGSDATL
CEEECCCCCCCCCEEEEECCCCCHHHHHCCCCCCCEEEEEEECCCCHHCCCCCCCCCEEE
LLIYNAHFDVVNFTLPSVPEGHNWLALIDTNQPDAQVQSLPFGHVYEVTGRSMLALGLSS
EEEEECCEEEEEEECCCCCCCCCEEEEEECCCCCCHHHCCCCCCEEEECCCEEEEECCCC
EQQPMRGLRHGLGALLDIAETPLG
CCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDQLTDRADALTQAAFSLRKAKISEGRPFPLGATWDGLGVNFAIFSAHATKVELCLFDET
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCC
GETELERIELPEYTDEVWHGYLPTARPGTVYGYRVHGPYEPDAGHRFNPNKLVIDPYAKQ
CCCCHHEECCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECHHHHH
LVGQLRWGPELFGYQLDHADKDLSFDDRDSAPLMLKCRVIDPAFTWGTARKPEIPWERTI
HHHHHCCCHHHHCEEECCCCCCCCCCCCCCCCEEEEEEEECCHHCCCCCCCCCCCCCEEE
FYEMHVKGFTKLHPLVPEADRGTFAGLAHQDIPAYLRSLGITSAELLPIHAFIDDSYLVD
EEEEECCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHEEEEEEEECCCHHHH
KGLRNYWGYNSIGFFAPEPRYLKTPLATEFKTMVNQFHANGIEVILDVVYNHTAEGNELG
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
PTLSFKGIDNASYYRLLPDKKRYYINDTGTGNTVNLSHQRVLQLVADSLRYWATEMRVDG
CEEEECCCCCCCEEEECCCCCEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCC
FRFDLATILAREPYGFDEGGGFLDACRQDPVLSGVKLIAEPWDIGPGGYQVGQFPPGWAE
EEEHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHCHHHEECCCCCCCCCCCCCCCCCCHHH
WNDKFRDTTRAFWKGDGGTLADFAKRISGSGDLFNKRGRRPWASVNFITAHDGFNLNDLV
HCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCEEEEEEECCCCCHHHHC
SYNDKHNEANGEDNRDGHSNNHSWNCGVEGPTDDAEIIALRERQKRNMLATMLLSHGTPM
CCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHEEEEHHHHHHHHHHHHHHHCCCCE
LLAGDEFGHTQDGNNNAYAQDNEISWLDWMGISAPGRQLREFTRKLIAMRKAFPILYRSR
EEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
FLVGSLNEELDVKDVTWLAPSGEEMATEQWTDGNARCFGMLLDGRAQETGVKRRGSDATL
CEEECCCCCCCCCEEEEECCCCCHHHHHCCCCCCCEEEEEEECCCCHHCCCCCCCCCEEE
LLIYNAHFDVVNFTLPSVPEGHNWLALIDTNQPDAQVQSLPFGHVYEVTGRSMLALGLSS
EEEEECCEEEEEEECCCCCCCCCEEEEEECCCCCCHHHCCCCCCEEEECCCEEEEECCCC
EQQPMRGLRHGLGALLDIAETPLG
CCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]