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The map label for this gene is sigW [H]

Identifier: 146341774

GI number: 146341774

Start: 5092552

End: 5093256

Strand: Reverse

Name: sigW [H]

Synonym: BRADO4891

Alternate gene names: 146341774

Gene position: 5093256-5092552 (Counterclockwise)

Preceding gene: 146341777

Following gene: 146341773

Centisome position: 68.31

GC content: 65.53

Gene sequence:

>705_bases
ATGAGCTACGCGCTGGACGTCAGAGCCCCGGCCGATGGCATGTCTTCGAGCGAGCCCGCGTCACCGGAGATCATGACGGT
GCCGTGGAGCGAGTCGGTCAGCACTGTTCCTGATGGCGCGCCGCCAAGCGAGGATGCGGTATTCGACGAGGACTCCGAAC
TGCTCGACCGGCTCGCGACCGGCGACGAGGTTGCGTTCCGCCTCCTCGTCGAGCGTCACATCGACCGGGCCTATGCGATC
GCGTTGCGGATCGTCGGGAGCGCGGCCGATGCCGAAGACGTCGTGCAGGACACGATGTTGAAGGTCTGGACTCATCGGGG
GCGATGGCAGCACGGTCGCGCGAAGTTCTCCACCTGGCTCTATCGCGTCGTCAGCAACCGCTGCATCGATCTGCGCCGCA
AGCCGCGGACGGAAAATGTCGATGTCGTGCCCGAGGTCCCCGACGGCAAGCCCGACGCGGTGAGCGTGATCGAGCGTGCG
CAGATGAGCGACCTGCTCGAGTCGGCGATGCAGCGTCTGCCGGAGCAGCAGCGCGTCGCCGTCATTCTGTCCTATCATGA
GAACATGAGCAACGGCGAGATCGCCGAGGTGATGGAGACAACGGTGACGGCGGTGGAGTCGCTGTTGAAGCGCGGCCGCC
AGCAGCTGCGCGAGATGCTGCGCCGGCACGAGCGGGACATCCGCGGCGCCTTTACTGATTGCTAA

Upstream 100 bases:

>100_bases
ACCACAAAAATATTCCGCCTGTTTATCTCGATGACGGCGTTCAACGATTGGAAGCCGTCTGCGAATTGCGCGTTCGGCAT
TGCGGGTTGGAGAGTCGGAA

Downstream 100 bases:

>100_bases
CCATAAGTTTCCCAGCGGAAGAGAATTCCCGCCTGAATCCCGCACGCGATTCCGTTTGATCGATCAGACGGGGCTGATCC
GCGCCGTCAGTCCTTCCACG

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSYALDVRAPADGMSSSEPASPEIMTVPWSESVSTVPDGAPPSEDAVFDEDSELLDRLATGDEVAFRLLVERHIDRAYAI
ALRIVGSAADAEDVVQDTMLKVWTHRGRWQHGRAKFSTWLYRVVSNRCIDLRRKPRTENVDVVPEVPDGKPDAVSVIERA
QMSDLLESAMQRLPEQQRVAVILSYHENMSNGEIAEVMETTVTAVESLLKRGRQQLREMLRRHERDIRGAFTDC

Sequences:

>Translated_234_residues
MSYALDVRAPADGMSSSEPASPEIMTVPWSESVSTVPDGAPPSEDAVFDEDSELLDRLATGDEVAFRLLVERHIDRAYAI
ALRIVGSAADAEDVVQDTMLKVWTHRGRWQHGRAKFSTWLYRVVSNRCIDLRRKPRTENVDVVPEVPDGKPDAVSVIERA
QMSDLLESAMQRLPEQQRVAVILSYHENMSNGEIAEVMETTVTAVESLLKRGRQQLREMLRRHERDIRGAFTDC
>Mature_233_residues
SYALDVRAPADGMSSSEPASPEIMTVPWSESVSTVPDGAPPSEDAVFDEDSELLDRLATGDEVAFRLLVERHIDRAYAIA
LRIVGSAADAEDVVQDTMLKVWTHRGRWQHGRAKFSTWLYRVVSNRCIDLRRKPRTENVDVVPEVPDGKPDAVSVIERAQ
MSDLLESAMQRLPEQQRVAVILSYHENMSNGEIAEVMETTVTAVESLLKRGRQQLREMLRRHERDIRGAFTDC

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=177, Percent_Identity=30.5084745762712, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 26347; Mature: 26216

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYALDVRAPADGMSSSEPASPEIMTVPWSESVSTVPDGAPPSEDAVFDEDSELLDRLAT
CCCEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
GDEVAFRLLVERHIDRAYAIALRIVGSAADAEDVVQDTMLKVWTHRGRWQHGRAKFSTWL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
YRVVSNRCIDLRRKPRTENVDVVPEVPDGKPDAVSVIERAQMSDLLESAMQRLPEQQRVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VILSYHENMSNGEIAEVMETTVTAVESLLKRGRQQLREMLRRHERDIRGAFTDC
HHEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SYALDVRAPADGMSSSEPASPEIMTVPWSESVSTVPDGAPPSEDAVFDEDSELLDRLAT
CCEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
GDEVAFRLLVERHIDRAYAIALRIVGSAADAEDVVQDTMLKVWTHRGRWQHGRAKFSTWL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
YRVVSNRCIDLRRKPRTENVDVVPEVPDGKPDAVSVIERAQMSDLLESAMQRLPEQQRVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VILSYHENMSNGEIAEVMETTVTAVESLLKRGRQQLREMLRRHERDIRGAFTDC
HHEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]