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The map label for this gene is asnB [H]

Identifier: 146341717

GI number: 146341717

Start: 5024849

End: 5026789

Strand: Reverse

Name: asnB [H]

Synonym: BRADO4822

Alternate gene names: 146341717

Gene position: 5026789-5024849 (Counterclockwise)

Preceding gene: 146341718

Following gene: 146341711

Centisome position: 67.41

GC content: 68.06

Gene sequence:

>1941_bases
ATGTGCGGTATTGCTGGAATCATCGGACGGCTGGACGACGATAACCGCGCAGCGCTGCAGCGCATGAACGGTGCCATGCT
CCATCGCGGTCCGGATGCCGGCGGCACGTGGGCCTCGGAGCCGGACGAGCGCGGCTGGGGGGCGTTGCTGGGACATCGCC
GGCTGGCGATCCTGGACCTGTCGCCGGCCGGCGTGCAACCGATGACGGATCCGGTGACCGGGCACGTCATCGTCTTCAAC
GGCGAGATCTACAATTTCGCTGATCTGCGCGAGCGCCTCGTCGCCGGCGGACAGCGGTTCACATCGACCGGTGACACCGC
CGTGATGTTGCGCGCCCTCGGCCTGCATGGGCCGGAGGCCGTGGCCTGGCTGCGCGGCATGTTTGCCTTTGCCTGCTGGG
ATCCGGGCCAGCGCCAGTTGCTGCTGGCGCGCGACCCGCTCGGCATCAAGCCGCTCTATGTGGCTCGCGCTGTGAACCAC
GACGCCGGCTGGTCGGTGGCCTTCGCATCGGAACTGCGCGCGCTGCTGGCGTCAGGGCTTCTCGGCACGCCAAGGCTCGA
CCCCGAGGCGGTCGCGAGCGGCGTTTGGAACGGCTTCGTGGTTGGTCCCGGCACGGCGGTGAAGGGCGTCGAACTGCTTC
CGCCCGGTCATCTGACGGCTTTCGACGGCGTCGGCAACACCGTGCGCGACGAGGATTTCTGGACCATCCCTCCGCTCGCG
CCAGAGCAGGGGATGGACGAGGAGCAACTCACGGACATCCTTGAGGAGACGCTGCGACTGCATCTCGCCAGCGACGTGCC
GCTGGCCGTCTTTCTCTCCGGCGGCATCGATTCCTCCGTCATGGCCAATCTGGCCCAGCGCGCAGCCCCAAATCCGATCC
ATACCTTCACGCTGGCCTTCGAGGAGCAGGAGCTCAACGAAGGGCCGATCGCGCGGCGCATCGCCACGGCGCTCGGCACC
GAGCATCACGAGGTGGTGCTGACCGAGCAGCACTTCGTCGACAATCTCGACGCCGCGCTCGACAGCCTGGATCAGCCGAC
CTTCGACGGTCTGAACGCCTACTACATGTCTCACGCCATCCGCAGCGCCGGGTTCACGGTGGCCTTGTCGGGCACGGGGG
GCGACGAACTGTTCGGCGGCTATACGTCATTCCGCGACCTGCCGGTCTTGCAGCAATGGTCGCAGCGCACATCTTGGATG
CCGGGCGGCCTGAAGGTCGCGGCCGCAACCCTGGCCTCACGGATGCTGTCGCCGTCCGGCGGGGCCGTTGCCGCGCAGAC
GCGCTGGGCCAAGCTGCCGGACATGGTCCGGCGCGGCGATGATCTGCTGGCGCTCTATCAGATGGCCTATGCGCTGTTCC
TCCCGGGCTTCCAGCGCGAGCTGCTCGCGCCCGACGTCGCCGCGACGGTGAGCGATGGCCTGCCGGCGGCGATGAAGGCG
AAGATCACCGCTGAGACGCGCTCTCGCACGCCGCTGTCGGCGATCAGCGTCATGGAGCAGCGCCTGTTCCTCGGCGAGCG
GCTGCTTCGCGACAATGACGTGGCCAGCATGGCTGCCTCGCTGGAGCAGCGCGTGCCGCTGGTCGATCAGGTGCTGCTCG
AAGCGGTCGACCGGCTGCCCGATCAGTCGCGCTACGAGCCGTTGCGCAGCAAGGCGATGTTGAGGCGCATCGGCCTCCGT
GGGCTCGATCTCTCGATGTTCGACCGGCCGAAGAGCGGCTTCGTGCTGCCGATGGATCGCTGGATCCGACGCGGCCTGCA
GGATGCGATGGACCAGACCCTGCGCGACCCGCAGGCCACGGCGCCCGTCGGTCTCGACCCCGCAGCGGTGGAACGCCTCT
GGCGCGCATTCCTCGACGGTGCGCCGGGACTGTATTGGTCGCGCGTGTGGTCGATCTATGTGTTCGTCCGGTGGTGCCAT
CGGCACCGCGTGTTCCGCTGA

Upstream 100 bases:

>100_bases
GGCGCGCGCGTCGGCATCATCGAGCTCGAGAAGCTCGCTCTCGACTTCGTTCTGCATCCTTTGTCGTTCTGATCTCGCGT
GTTGCAAACGGATTGGTTTG

Downstream 100 bases:

>100_bases
CCGGCGGCTCTGCCGTCGTGCTCAGCGCCAATCCGGCGTGGCGCTCAGGAAGGCCTGGCGCAGCGAGCCCACATTTTCCG
GGTCGAACAGCGTGTGATGC

Product: putative asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MCGIAGIIGRLDDDNRAALQRMNGAMLHRGPDAGGTWASEPDERGWGALLGHRRLAILDLSPAGVQPMTDPVTGHVIVFN
GEIYNFADLRERLVAGGQRFTSTGDTAVMLRALGLHGPEAVAWLRGMFAFACWDPGQRQLLLARDPLGIKPLYVARAVNH
DAGWSVAFASELRALLASGLLGTPRLDPEAVASGVWNGFVVGPGTAVKGVELLPPGHLTAFDGVGNTVRDEDFWTIPPLA
PEQGMDEEQLTDILEETLRLHLASDVPLAVFLSGGIDSSVMANLAQRAAPNPIHTFTLAFEEQELNEGPIARRIATALGT
EHHEVVLTEQHFVDNLDAALDSLDQPTFDGLNAYYMSHAIRSAGFTVALSGTGGDELFGGYTSFRDLPVLQQWSQRTSWM
PGGLKVAAATLASRMLSPSGGAVAAQTRWAKLPDMVRRGDDLLALYQMAYALFLPGFQRELLAPDVAATVSDGLPAAMKA
KITAETRSRTPLSAISVMEQRLFLGERLLRDNDVASMAASLEQRVPLVDQVLLEAVDRLPDQSRYEPLRSKAMLRRIGLR
GLDLSMFDRPKSGFVLPMDRWIRRGLQDAMDQTLRDPQATAPVGLDPAAVERLWRAFLDGAPGLYWSRVWSIYVFVRWCH
RHRVFR

Sequences:

>Translated_646_residues
MCGIAGIIGRLDDDNRAALQRMNGAMLHRGPDAGGTWASEPDERGWGALLGHRRLAILDLSPAGVQPMTDPVTGHVIVFN
GEIYNFADLRERLVAGGQRFTSTGDTAVMLRALGLHGPEAVAWLRGMFAFACWDPGQRQLLLARDPLGIKPLYVARAVNH
DAGWSVAFASELRALLASGLLGTPRLDPEAVASGVWNGFVVGPGTAVKGVELLPPGHLTAFDGVGNTVRDEDFWTIPPLA
PEQGMDEEQLTDILEETLRLHLASDVPLAVFLSGGIDSSVMANLAQRAAPNPIHTFTLAFEEQELNEGPIARRIATALGT
EHHEVVLTEQHFVDNLDAALDSLDQPTFDGLNAYYMSHAIRSAGFTVALSGTGGDELFGGYTSFRDLPVLQQWSQRTSWM
PGGLKVAAATLASRMLSPSGGAVAAQTRWAKLPDMVRRGDDLLALYQMAYALFLPGFQRELLAPDVAATVSDGLPAAMKA
KITAETRSRTPLSAISVMEQRLFLGERLLRDNDVASMAASLEQRVPLVDQVLLEAVDRLPDQSRYEPLRSKAMLRRIGLR
GLDLSMFDRPKSGFVLPMDRWIRRGLQDAMDQTLRDPQATAPVGLDPAAVERLWRAFLDGAPGLYWSRVWSIYVFVRWCH
RHRVFR
>Mature_646_residues
MCGIAGIIGRLDDDNRAALQRMNGAMLHRGPDAGGTWASEPDERGWGALLGHRRLAILDLSPAGVQPMTDPVTGHVIVFN
GEIYNFADLRERLVAGGQRFTSTGDTAVMLRALGLHGPEAVAWLRGMFAFACWDPGQRQLLLARDPLGIKPLYVARAVNH
DAGWSVAFASELRALLASGLLGTPRLDPEAVASGVWNGFVVGPGTAVKGVELLPPGHLTAFDGVGNTVRDEDFWTIPPLA
PEQGMDEEQLTDILEETLRLHLASDVPLAVFLSGGIDSSVMANLAQRAAPNPIHTFTLAFEEQELNEGPIARRIATALGT
EHHEVVLTEQHFVDNLDAALDSLDQPTFDGLNAYYMSHAIRSAGFTVALSGTGGDELFGGYTSFRDLPVLQQWSQRTSWM
PGGLKVAAATLASRMLSPSGGAVAAQTRWAKLPDMVRRGDDLLALYQMAYALFLPGFQRELLAPDVAATVSDGLPAAMKA
KITAETRSRTPLSAISVMEQRLFLGERLLRDNDVASMAASLEQRVPLVDQVLLEAVDRLPDQSRYEPLRSKAMLRRIGLR
GLDLSMFDRPKSGFVLPMDRWIRRGLQDAMDQTLRDPQATAPVGLDPAAVERLWRAFLDGAPGLYWSRVWSIYVFVRWCH
RHRVFR

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=408, Percent_Identity=26.9607843137255, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI168229252, Length=408, Percent_Identity=26.9607843137255, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI168229250, Length=408, Percent_Identity=26.9607843137255, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI296010848, Length=381, Percent_Identity=26.509186351706, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI296010852, Length=288, Percent_Identity=26.3888888888889, Blast_Score=93, Evalue=9e-19,
Organism=Homo sapiens, GI296010850, Length=288, Percent_Identity=26.3888888888889, Blast_Score=93, Evalue=9e-19,
Organism=Escherichia coli, GI1786889, Length=445, Percent_Identity=29.2134831460674, Blast_Score=168, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI71993933, Length=413, Percent_Identity=27.8450363196126, Blast_Score=139, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI25147557, Length=413, Percent_Identity=27.8450363196126, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17560178, Length=410, Percent_Identity=27.0731707317073, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321563, Length=453, Percent_Identity=26.2693156732892, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325403, Length=451, Percent_Identity=27.2727272727273, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45553209, Length=419, Percent_Identity=27.6849642004773, Blast_Score=143, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70586; Mature: 70586

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGIIGRLDDDNRAALQRMNGAMLHRGPDAGGTWASEPDERGWGALLGHRRLAILDL
CCCHHHHHHCCCCCHHHHHHHHCCHHEECCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEC
SPAGVQPMTDPVTGHVIVFNGEIYNFADLRERLVAGGQRFTSTGDTAVMLRALGLHGPEA
CCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHH
VAWLRGMFAFACWDPGQRQLLLARDPLGIKPLYVARAVNHDAGWSVAFASELRALLASGL
HHHHHHHHHHEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
LGTPRLDPEAVASGVWNGFVVGPGTAVKGVELLPPGHLTAFDGVGNTVRDEDFWTIPPLA
CCCCCCCHHHHHHHCCCCEEECCCCCCCCEEECCCCCCEEECCCCCCCCCCCCEECCCCC
PEQGMDEEQLTDILEETLRLHLASDVPLAVFLSGGIDSSVMANLAQRAAPNPIHTFTLAF
CCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEE
EEQELNEGPIARRIATALGTEHHEVVLTEQHFVDNLDAALDSLDQPTFDGLNAYYMSHAI
EHHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RSAGFTVALSGTGGDELFGGYTSFRDLPVLQQWSQRTSWMPGGLKVAAATLASRMLSPSG
HHCCCEEEEECCCCHHHHCCHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
GAVAAQTRWAKLPDMVRRGDDLLALYQMAYALFLPGFQRELLAPDVAATVSDGLPAAMKA
CEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHCCCCCHHHHE
KITAETRSRTPLSAISVMEQRLFLGERLLRDNDVASMAASLEQRVPLVDQVLLEAVDRLP
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC
DQSRYEPLRSKAMLRRIGLRGLDLSMFDRPKSGFVLPMDRWIRRGLQDAMDQTLRDPQAT
CCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCC
APVGLDPAAVERLWRAFLDGAPGLYWSRVWSIYVFVRWCHRHRVFR
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCGIAGIIGRLDDDNRAALQRMNGAMLHRGPDAGGTWASEPDERGWGALLGHRRLAILDL
CCCHHHHHHCCCCCHHHHHHHHCCHHEECCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEC
SPAGVQPMTDPVTGHVIVFNGEIYNFADLRERLVAGGQRFTSTGDTAVMLRALGLHGPEA
CCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHH
VAWLRGMFAFACWDPGQRQLLLARDPLGIKPLYVARAVNHDAGWSVAFASELRALLASGL
HHHHHHHHHHEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
LGTPRLDPEAVASGVWNGFVVGPGTAVKGVELLPPGHLTAFDGVGNTVRDEDFWTIPPLA
CCCCCCCHHHHHHHCCCCEEECCCCCCCCEEECCCCCCEEECCCCCCCCCCCCEECCCCC
PEQGMDEEQLTDILEETLRLHLASDVPLAVFLSGGIDSSVMANLAQRAAPNPIHTFTLAF
CCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEE
EEQELNEGPIARRIATALGTEHHEVVLTEQHFVDNLDAALDSLDQPTFDGLNAYYMSHAI
EHHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RSAGFTVALSGTGGDELFGGYTSFRDLPVLQQWSQRTSWMPGGLKVAAATLASRMLSPSG
HHCCCEEEEECCCCHHHHCCHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
GAVAAQTRWAKLPDMVRRGDDLLALYQMAYALFLPGFQRELLAPDVAATVSDGLPAAMKA
CEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHCCCCCHHHHE
KITAETRSRTPLSAISVMEQRLFLGERLLRDNDVASMAASLEQRVPLVDQVLLEAVDRLP
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC
DQSRYEPLRSKAMLRRIGLRGLDLSMFDRPKSGFVLPMDRWIRRGLQDAMDQTLRDPQAT
CCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCC
APVGLDPAAVERLWRAFLDGAPGLYWSRVWSIYVFVRWCHRHRVFR
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]