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The map label for this gene is groEL [H]

Identifier: 146341267

GI number: 146341267

Start: 4534807

End: 4536435

Strand: Reverse

Name: groEL [H]

Synonym: BRADO4344

Alternate gene names: 146341267

Gene position: 4536435-4534807 (Counterclockwise)

Preceding gene: 146341268

Following gene: 146341262

Centisome position: 60.84

GC content: 67.03

Gene sequence:

>1629_bases
ATGCCGAAGATCATGCTGCATGACGAAGCGGCGCGAGCCGCTTTGGGACGGGGCGTTGCCAAGCTCGCCAAGGCGGTGCG
CGGCACCCTGGGTCCGAAGGGAATGAACGCGATCATGGACCGCCCGATCGGGACGCCGATCGTGTCGCGCGACGGCGTCA
GCATCGCCGCCGAAATCGAGCTGGAATGTCCGTTCGAGAACATGGGCGCGCAGGTGCTGCGCGAGGTCTCGAAGCAAACC
AATGACACCGCCGGCGACGGTACCACGACGGCCACGGTGCTGGCCGACGTGCTGGTGCAGGACGGGCTGAAATGCCTCGC
GGCAGGGGCCAATCCGGTGGAACTGGTCGAGGGACTCGAGCTTGCGGTCGCCGAGACGATCAAGCACCTGAAGCGCTCCG
CGATCGCGGTGCAGGGCTCGGCGGGACTGCGCGCGGTCGCGAGCATCGCGGCCAACGATGCCGCGCTCGGCGACATGGTC
GCGGAAGCCTTCGAGCGCGCGGGGACGCATGGCATCGTCGCGGTCGAATATGGCAGCACGGTCCAGACGACGCTCGAAGT
GATCGAAGGCATGGCGTTCGAGCGCGGCTATCTCTCGCATCACATGGTAACGGATGTGGAGCGCATGCAGGTCGTGCTCG
ACAATCCGTTCATCCTGATGACGGACCACAAGATCCAGAGCGGCGAGCAGCTCGCCGGCGTGATCTCGCTGGTCGAGAAG
AGCGGCAGGCCGCTCTTGATCATCGCCGAGGAGGTCGCGCCGGTCGTGATCATGCAGCTCCTGGCGCGCCGCGAGAAGAA
CAACTTCAAGGTCGCCGCGATCCATCCGCCAGAGTTCGGCCATTGGCGCAAGGCCATGCTCGAGGATATCGCGATCACCA
CGGGCGGCCGCGTGATCTCCGTCGATCTCGGCGGCCGGCTGGAAAAGGCCGAGCTGCAGGATCTCGGCGCCGCGCGCCAG
GTGCGGATCTCCGCTTCCAAGACCTTGATCACGGCAGGCGCCGGCGATCCCGATCGGATTGCCGCGCGCCGCGAGCAGGT
GATGCGGCAATATGAAGCCGCGCCGGAAAACATCGAGCGCGACAAGTTCCAGGAGCGCATTGCAAAGCTCTCGGGCGGCA
CGGCGATGATCCTCGCCGGCGGCGCAACGCCGGTCGAGCAGAAGCGCCGCACCCAGCTGATCGAGGACGCGATCAATGCG
ACGCGGGCGGCGATCGAGGAGGGCATCGTCCCCGGCGGCGGTTTCGCGCTGCTGAAGGCGGCGCCGAAGCTCGATGAGCT
GATCAGTGGATTGGACGGCAGCGTGCGGCAGGGGGCCGAGCTGCTGCAGCGCTCGCTGAGCCGGCCGTTGTTCCACATCG
CATCGAATGCGGGCCTCGACGCCGAGGCGGAGGTCAGGAAGGTGGCGAAGGGCGCCAATGGCCACGGCCTCGACGCGCGC
AACGGCGCCTCGGTCGATCTCATCAAGGCCGGCATCATCGATCCCGTCAAGGTCTGCTATAGCGCGGTGCGCAACGCCGC
CTCCGTCGCCGGGCTGATCCTGACGACGCAGACTTTGATCGCGAAGAAGCCGGACGATTACGACCCGACCGCGGGTCCCG
CCTTGGGCGGCGGCGCCGAGCTGTTGTGA

Upstream 100 bases:

>100_bases
TACGTTCCGAAAGCACGCGCCTCGGCCTGAACGGCCGGGGGACAACAAAAGTCGCGCCGGCCTGAACGGCGAACGACCAG
GCGAGAGGGAGGTTGAGACC

Downstream 100 bases:

>100_bases
GCGCGCAGGGCTTTCGCTGATGACGGACCGGGCGTTCGCGCCCGGCTGCGGCGGACTGATCGTGCGTCTCTCCGTTCGAG
CCTCGTCACGGCGACGGGCG

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 4; Protein Cpn60 4 [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MPKIMLHDEAARAALGRGVAKLAKAVRGTLGPKGMNAIMDRPIGTPIVSRDGVSIAAEIELECPFENMGAQVLREVSKQT
NDTAGDGTTTATVLADVLVQDGLKCLAAGANPVELVEGLELAVAETIKHLKRSAIAVQGSAGLRAVASIAANDAALGDMV
AEAFERAGTHGIVAVEYGSTVQTTLEVIEGMAFERGYLSHHMVTDVERMQVVLDNPFILMTDHKIQSGEQLAGVISLVEK
SGRPLLIIAEEVAPVVIMQLLARREKNNFKVAAIHPPEFGHWRKAMLEDIAITTGGRVISVDLGGRLEKAELQDLGAARQ
VRISASKTLITAGAGDPDRIAARREQVMRQYEAAPENIERDKFQERIAKLSGGTAMILAGGATPVEQKRRTQLIEDAINA
TRAAIEEGIVPGGGFALLKAAPKLDELISGLDGSVRQGAELLQRSLSRPLFHIASNAGLDAEAEVRKVAKGANGHGLDAR
NGASVDLIKAGIIDPVKVCYSAVRNAASVAGLILTTQTLIAKKPDDYDPTAGPALGGGAELL

Sequences:

>Translated_542_residues
MPKIMLHDEAARAALGRGVAKLAKAVRGTLGPKGMNAIMDRPIGTPIVSRDGVSIAAEIELECPFENMGAQVLREVSKQT
NDTAGDGTTTATVLADVLVQDGLKCLAAGANPVELVEGLELAVAETIKHLKRSAIAVQGSAGLRAVASIAANDAALGDMV
AEAFERAGTHGIVAVEYGSTVQTTLEVIEGMAFERGYLSHHMVTDVERMQVVLDNPFILMTDHKIQSGEQLAGVISLVEK
SGRPLLIIAEEVAPVVIMQLLARREKNNFKVAAIHPPEFGHWRKAMLEDIAITTGGRVISVDLGGRLEKAELQDLGAARQ
VRISASKTLITAGAGDPDRIAARREQVMRQYEAAPENIERDKFQERIAKLSGGTAMILAGGATPVEQKRRTQLIEDAINA
TRAAIEEGIVPGGGFALLKAAPKLDELISGLDGSVRQGAELLQRSLSRPLFHIASNAGLDAEAEVRKVAKGANGHGLDAR
NGASVDLIKAGIIDPVKVCYSAVRNAASVAGLILTTQTLIAKKPDDYDPTAGPALGGGAELL
>Mature_541_residues
PKIMLHDEAARAALGRGVAKLAKAVRGTLGPKGMNAIMDRPIGTPIVSRDGVSIAAEIELECPFENMGAQVLREVSKQTN
DTAGDGTTTATVLADVLVQDGLKCLAAGANPVELVEGLELAVAETIKHLKRSAIAVQGSAGLRAVASIAANDAALGDMVA
EAFERAGTHGIVAVEYGSTVQTTLEVIEGMAFERGYLSHHMVTDVERMQVVLDNPFILMTDHKIQSGEQLAGVISLVEKS
GRPLLIIAEEVAPVVIMQLLARREKNNFKVAAIHPPEFGHWRKAMLEDIAITTGGRVISVDLGGRLEKAELQDLGAARQV
RISASKTLITAGAGDPDRIAARREQVMRQYEAAPENIERDKFQERIAKLSGGTAMILAGGATPVEQKRRTQLIEDAINAT
RAAIEEGIVPGGGFALLKAAPKLDELISGLDGSVRQGAELLQRSLSRPLFHIASNAGLDAEAEVRKVAKGANGHGLDARN
GASVDLIKAGIIDPVKVCYSAVRNAASVAGLILTTQTLIAKKPDDYDPTAGPALGGGAELL

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=522, Percent_Identity=37.1647509578544, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI31542947, Length=522, Percent_Identity=37.1647509578544, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI63162572, Length=546, Percent_Identity=23.992673992674, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1790586, Length=517, Percent_Identity=44.294003868472, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17555558, Length=516, Percent_Identity=36.6279069767442, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI193210679, Length=218, Percent_Identity=37.1559633027523, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25144674, Length=546, Percent_Identity=23.992673992674, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323288, Length=515, Percent_Identity=38.4466019417476, Blast_Score=353, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6322524, Length=183, Percent_Identity=25.6830601092896, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24641193, Length=516, Percent_Identity=39.7286821705426, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24641191, Length=516, Percent_Identity=39.7286821705426, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI45550936, Length=516, Percent_Identity=37.984496124031, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI45550132, Length=516, Percent_Identity=37.984496124031, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI45550935, Length=516, Percent_Identity=37.984496124031, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI17864606, Length=534, Percent_Identity=35.2059925093633, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24584129, Length=534, Percent_Identity=31.4606741573034, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI19921262, Length=534, Percent_Identity=31.4606741573034, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24652903, Length=543, Percent_Identity=25.414364640884, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17647245, Length=522, Percent_Identity=25.095785440613, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57024; Mature: 56893

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKIMLHDEAARAALGRGVAKLAKAVRGTLGPKGMNAIMDRPIGTPIVSRDGVSIAAEIE
CCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEE
LECPFENMGAQVLREVSKQTNDTAGDGTTTATVLADVLVQDGLKCLAAGANPVELVEGLE
EECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHH
LAVAETIKHLKRSAIAVQGSAGLRAVASIAANDAALGDMVAEAFERAGTHGIVAVEYGST
HHHHHHHHHHHHHHEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEECCCH
VQTTLEVIEGMAFERGYLSHHMVTDVERMQVVLDNPFILMTDHKIQSGEQLAGVISLVEK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHH
SGRPLLIIAEEVAPVVIMQLLARREKNNFKVAAIHPPEFGHWRKAMLEDIAITTGGRVIS
CCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHEECCCEEEE
VDLGGRLEKAELQDLGAARQVRISASKTLITAGAGDPDRIAARREQVMRQYEAAPENIER
EECCCCCCHHHHHHCCCHHEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHH
DKFQERIAKLSGGTAMILAGGATPVEQKRRTQLIEDAINATRAAIEEGIVPGGGFALLKA
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHC
APKLDELISGLDGSVRQGAELLQRSLSRPLFHIASNAGLDAEAEVRKVAKGANGHGLDAR
CCCHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCC
NGASVDLIKAGIIDPVKVCYSAVRNAASVAGLILTTQTLIAKKPDDYDPTAGPALGGGAE
CCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LL
CC
>Mature Secondary Structure 
PKIMLHDEAARAALGRGVAKLAKAVRGTLGPKGMNAIMDRPIGTPIVSRDGVSIAAEIE
CCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEE
LECPFENMGAQVLREVSKQTNDTAGDGTTTATVLADVLVQDGLKCLAAGANPVELVEGLE
EECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHH
LAVAETIKHLKRSAIAVQGSAGLRAVASIAANDAALGDMVAEAFERAGTHGIVAVEYGST
HHHHHHHHHHHHHHEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEECCCH
VQTTLEVIEGMAFERGYLSHHMVTDVERMQVVLDNPFILMTDHKIQSGEQLAGVISLVEK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHH
SGRPLLIIAEEVAPVVIMQLLARREKNNFKVAAIHPPEFGHWRKAMLEDIAITTGGRVIS
CCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHEECCCEEEE
VDLGGRLEKAELQDLGAARQVRISASKTLITAGAGDPDRIAARREQVMRQYEAAPENIER
EECCCCCCHHHHHHCCCHHEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHH
DKFQERIAKLSGGTAMILAGGATPVEQKRRTQLIEDAINATRAAIEEGIVPGGGFALLKA
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHC
APKLDELISGLDGSVRQGAELLQRSLSRPLFHIASNAGLDAEAEVRKVAKGANGHGLDAR
CCCHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCC
NGASVDLIKAGIIDPVKVCYSAVRNAASVAGLILTTQTLIAKKPDDYDPTAGPALGGGAE
CCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]