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The map label for this gene is nfrA1 [H]

Identifier: 146341205

GI number: 146341205

Start: 4480544

End: 4481398

Strand: Reverse

Name: nfrA1 [H]

Synonym: BRADO4279

Alternate gene names: 146341205

Gene position: 4481398-4480544 (Counterclockwise)

Preceding gene: 146341206

Following gene: 146341202

Centisome position: 60.1

GC content: 69.36

Gene sequence:

>855_bases
ATGACGATCATCGAAACGAGGGCACCGACCACTCCAAGCCACGACATCACGGCGGCGCTGCGCAAGCGCTACGGCACTGA
CATCCCCGCCGTAGCGGCCGGCGCCGATGACATCCTCGGCCATCTCCTGGCGCACCGCTCGATCCGCAGCTACCGCCCCG
ATCCGGTGCCGCCGCACACCGTGGAAACGCTGGTGGCCGCGGCGCAGTCGGCGGCGTCGTCGTCGAACCTGCAGACCTGG
AGCGTCGTCGCGGTCGAGGACCCCGCGCGCAAGCAGCGCCTGTCGGAATTCGTGGGCAATCAGAAGCACGTCCGCGAGGC
ACCGCTTTTCCTGGTCTGGCTCGCCGATCTGTCGCGCGCCGAGCGGCTGGCGCGGCGGGAAGGGCGCCCCGATGACGGCA
TCCATTTCCTGGAGACGCTGTTCGTCGCGATCATCGACGCAGCGCTCGCTGCGCAGAATGCCGTCGTCGCGCTCGAGGCG
CTCGGCCTCGGCTCGGTCTATATCGGCGCGATCCGCAACCGGCCGCAGGCGGTGGCTGAGGAGCTCGGCCTGCCGCCGAA
CGTGCTCGCCGTGTTCGGCCTCTGCGTCGGCTATGCCGATCTCGCGGCCGGCGAGGACGTCAAACCGCGCCTGCGCCAGA
GCGTGGTTCTGCATCGCGAGCGCTATGCGCCGACCGATGAGATCCACGGCATCGCCGCCTATGACGAGACTTTGCGCGCG
TTCCAGACCAGCCAGGGCGTGGCGCCGGTCGGCTGGCGGGACACGGTGCTCAACCGGCTCGGCTCGGCGAAGGCGCTCAA
TGGCCGCGACAAACTGGCTGAGGCGCTGCGCGCGCTCGGCTTCGGATTGAAGTAA

Upstream 100 bases:

>100_bases
TTGCCGTTGTCCTCGGGGTCGGCTTTGCTAGGCGGCGACGCGCACGCACTTATCGAATATCGGCCGTCACAATGACGTAC
GGGCCGGCATGGGGACAGGA

Downstream 100 bases:

>100_bases
CTGCGGACCCGGAGAGATTCGCGGCGACCCGGGACGGACCGCCGCGATGGCTCACTTCATGTAGTCGGGCAGGGTGAAGC
CGTCATAGAAGCGATCCGCG

Product: putative nitroreductase family protein oxygen-insensitive NADPH nitroreductase (NfsA-like)

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTIIETRAPTTPSHDITAALRKRYGTDIPAVAAGADDILGHLLAHRSIRSYRPDPVPPHTVETLVAAAQSAASSSNLQTW
SVVAVEDPARKQRLSEFVGNQKHVREAPLFLVWLADLSRAERLARREGRPDDGIHFLETLFVAIIDAALAAQNAVVALEA
LGLGSVYIGAIRNRPQAVAEELGLPPNVLAVFGLCVGYADLAAGEDVKPRLRQSVVLHRERYAPTDEIHGIAAYDETLRA
FQTSQGVAPVGWRDTVLNRLGSAKALNGRDKLAEALRALGFGLK

Sequences:

>Translated_284_residues
MTIIETRAPTTPSHDITAALRKRYGTDIPAVAAGADDILGHLLAHRSIRSYRPDPVPPHTVETLVAAAQSAASSSNLQTW
SVVAVEDPARKQRLSEFVGNQKHVREAPLFLVWLADLSRAERLARREGRPDDGIHFLETLFVAIIDAALAAQNAVVALEA
LGLGSVYIGAIRNRPQAVAEELGLPPNVLAVFGLCVGYADLAAGEDVKPRLRQSVVLHRERYAPTDEIHGIAAYDETLRA
FQTSQGVAPVGWRDTVLNRLGSAKALNGRDKLAEALRALGFGLK
>Mature_283_residues
TIIETRAPTTPSHDITAALRKRYGTDIPAVAAGADDILGHLLAHRSIRSYRPDPVPPHTVETLVAAAQSAASSSNLQTWS
VVAVEDPARKQRLSEFVGNQKHVREAPLFLVWLADLSRAERLARREGRPDDGIHFLETLFVAIIDAALAAQNAVVALEAL
GLGSVYIGAIRNRPQAVAEELGLPPNVLAVFGLCVGYADLAAGEDVKPRLRQSVVLHRERYAPTDEIHGIAAYDETLRAF
QTSQGVAPVGWRDTVLNRLGSAKALNGRDKLAEALRALGFGLK

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=205, Percent_Identity=38.5365853658537, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 30620; Mature: 30489

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIETRAPTTPSHDITAALRKRYGTDIPAVAAGADDILGHLLAHRSIRSYRPDPVPPHT
CCEECCCCCCCCCHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHH
VETLVAAAQSAASSSNLQTWSVVAVEDPARKQRLSEFVGNQKHVREAPLFLVWLADLSRA
HHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHH
ERLARREGRPDDGIHFLETLFVAIIDAALAAQNAVVALEALGLGSVYIGAIRNRPQAVAE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHH
ELGLPPNVLAVFGLCVGYADLAAGEDVKPRLRQSVVLHRERYAPTDEIHGIAAYDETLRA
HCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHH
FQTSQGVAPVGWRDTVLNRLGSAKALNGRDKLAEALRALGFGLK
HHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TIIETRAPTTPSHDITAALRKRYGTDIPAVAAGADDILGHLLAHRSIRSYRPDPVPPHT
CEECCCCCCCCCHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHH
VETLVAAAQSAASSSNLQTWSVVAVEDPARKQRLSEFVGNQKHVREAPLFLVWLADLSRA
HHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHH
ERLARREGRPDDGIHFLETLFVAIIDAALAAQNAVVALEALGLGSVYIGAIRNRPQAVAE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHH
ELGLPPNVLAVFGLCVGYADLAAGEDVKPRLRQSVVLHRERYAPTDEIHGIAAYDETLRA
HCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHH
FQTSQGVAPVGWRDTVLNRLGSAKALNGRDKLAEALRALGFGLK
HHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]