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The map label for this gene is nisX1 [H]

Identifier: 146341193

GI number: 146341193

Start: 4469419

End: 4470216

Strand: Reverse

Name: nisX1 [H]

Synonym: BRADO4267

Alternate gene names: 146341193

Gene position: 4470216-4469419 (Counterclockwise)

Preceding gene: 146341194

Following gene: 146341191

Centisome position: 59.95

GC content: 61.65

Gene sequence:

>798_bases
TTGCCTCGAGCGACTTATTACCGGCATCTCAATCGACGCAGCCGCGCCGAGGCCGAGTGCGAGTTGCGCGACCAGCTCCA
GCGCATCTGCCTGAAGCATCCGTTCTACGGCTATCGACGGGTGACCGCCGCTCTTCGGCGTCTGGGGATGGCCGTCAATG
CCAAGAAAGTCCTGCGGCTCATGCGGCAGGATAATCTTCTCGCGCAGCGCAAGACGCCGTTCCTGAAGCCGCCGACTGAG
AGGCCGACTGACGTGATCGTCGTCCCCAACCTGATCCGTGGCCTGGCGCCATCGGCTCCGGATCAGATCTGGGTCGCTGA
CATCACCTACGTCCATCTCGCCAAGACGTTCGCCTATCTCGCCGTCATTCTCGACGGCTTCTCCCGCAAGGCGGTCGGCT
GGGCATTTGACAACACCCTCGACGCCTCGCTCGCCATCGCGGCGCTCGACAAAGCGCTCAAATCCCGGAATCCCAAGCCG
GGCAGCCTGATCCACCACTCCGACCGCGGCGTGCAATACGCCTCGATCGCTTATCGCCAGCGCCTCGCCGATCGCGAGAT
CAAGATCAGCATGAGCAGTCCAGGCAACCCCTTCGACAACGCCAAGGCCGAAAGCTTCATGAAGACGCTCAAGGCCGAGG
AGGTCAACGGCAAGACCTTTGCCGACGTCAACGACGCCCGGCGCCGGATCAACAGCTTCATCGCCGAGATCTACAACAAG
GAGCGCTTGCATTCGGCCCTCGGCTATCGATCTCCCCTTGAGTTCGAAGCCGCGTTCAAGCAAAACACAATGCGATGA

Upstream 100 bases:

>100_bases
AAGGCAAACCCGTATCCGCATCATCGAAGTCATCAAAAGCATGACCGCGCAGACAGCCGACTCCAGCGCTCATGTGCAGC
GCCTCTGCGCTTTGGCAGGG

Downstream 100 bases:

>100_bases
CTTCACCCACTGTCACTGAGATTATCGTGTCTCACTTGAGGGGTGCAGTCCAGTGCATCAAACTGACGTCGCATCGCTCA
TACCGCCCAGTCCAGGACCT

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MPRATYYRHLNRRSRAEAECELRDQLQRICLKHPFYGYRRVTAALRRLGMAVNAKKVLRLMRQDNLLAQRKTPFLKPPTE
RPTDVIVVPNLIRGLAPSAPDQIWVADITYVHLAKTFAYLAVILDGFSRKAVGWAFDNTLDASLAIAALDKALKSRNPKP
GSLIHHSDRGVQYASIAYRQRLADREIKISMSSPGNPFDNAKAESFMKTLKAEEVNGKTFADVNDARRRINSFIAEIYNK
ERLHSALGYRSPLEFEAAFKQNTMR

Sequences:

>Translated_265_residues
MPRATYYRHLNRRSRAEAECELRDQLQRICLKHPFYGYRRVTAALRRLGMAVNAKKVLRLMRQDNLLAQRKTPFLKPPTE
RPTDVIVVPNLIRGLAPSAPDQIWVADITYVHLAKTFAYLAVILDGFSRKAVGWAFDNTLDASLAIAALDKALKSRNPKP
GSLIHHSDRGVQYASIAYRQRLADREIKISMSSPGNPFDNAKAESFMKTLKAEEVNGKTFADVNDARRRINSFIAEIYNK
ERLHSALGYRSPLEFEAAFKQNTMR
>Mature_264_residues
PRATYYRHLNRRSRAEAECELRDQLQRICLKHPFYGYRRVTAALRRLGMAVNAKKVLRLMRQDNLLAQRKTPFLKPPTER
PTDVIVVPNLIRGLAPSAPDQIWVADITYVHLAKTFAYLAVILDGFSRKAVGWAFDNTLDASLAIAALDKALKSRNPKPG
SLIHHSDRGVQYASIAYRQRLADREIKISMSSPGNPFDNAKAESFMKTLKAEEVNGKTFADVNDARRRINSFIAEIYNKE
RLHSALGYRSPLEFEAAFKQNTMR

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=225, Percent_Identity=32.4444444444444, Blast_Score=112, Evalue=2e-25,
Organism=Escherichia coli, GI1788405, Length=262, Percent_Identity=33.969465648855, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1787263, Length=262, Percent_Identity=33.969465648855, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1786753, Length=262, Percent_Identity=33.969465648855, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1786570, Length=262, Percent_Identity=33.969465648855, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1786490, Length=262, Percent_Identity=33.969465648855, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1789981, Length=257, Percent_Identity=30.7392996108949, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790724, Length=259, Percent_Identity=31.2741312741313, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789424, Length=259, Percent_Identity=31.2741312741313, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789224, Length=259, Percent_Identity=31.2741312741313, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788305, Length=259, Percent_Identity=31.2741312741313, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787669, Length=259, Percent_Identity=31.2741312741313, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786558, Length=259, Percent_Identity=31.2741312741313, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 30188; Mature: 30057

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRATYYRHLNRRSRAEAECELRDQLQRICLKHPFYGYRRVTAALRRLGMAVNAKKVLRL
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHH
MRQDNLLAQRKTPFLKPPTERPTDVIVVPNLIRGLAPSAPDQIWVADITYVHLAKTFAYL
HHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHH
AVILDGFSRKAVGWAFDNTLDASLAIAALDKALKSRNPKPGSLIHHSDRGVQYASIAYRQ
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHH
RLADREIKISMSSPGNPFDNAKAESFMKTLKAEEVNGKTFADVNDARRRINSFIAEIYNK
HHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ERLHSALGYRSPLEFEAAFKQNTMR
HHHHHHHCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
PRATYYRHLNRRSRAEAECELRDQLQRICLKHPFYGYRRVTAALRRLGMAVNAKKVLRL
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHH
MRQDNLLAQRKTPFLKPPTERPTDVIVVPNLIRGLAPSAPDQIWVADITYVHLAKTFAYL
HHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHH
AVILDGFSRKAVGWAFDNTLDASLAIAALDKALKSRNPKPGSLIHHSDRGVQYASIAYRQ
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHH
RLADREIKISMSSPGNPFDNAKAESFMKTLKAEEVNGKTFADVNDARRRINSFIAEIYNK
HHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ERLHSALGYRSPLEFEAAFKQNTMR
HHHHHHHCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1482189; 11337471; 1482192 [H]