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The map label for this gene is hipO [H]

Identifier: 146341140

GI number: 146341140

Start: 4412046

End: 4413215

Strand: Reverse

Name: hipO [H]

Synonym: BRADO4211

Alternate gene names: 146341140

Gene position: 4413215-4412046 (Counterclockwise)

Preceding gene: 146341142

Following gene: 146341138

Centisome position: 59.19

GC content: 68.72

Gene sequence:

>1170_bases
ATGCCGATCATCAACCGCGCCGCCGAGCTCGCCGAGGATGCCAAGGTCTGGCGCCGTGATTTCCACCAGCATCCCGAGCT
GCAATACGAGGTGCATCGGACGGCCGCGCGCGTCGCCGAGCTGCTGACCTCATTCGGTGTCGACGAGGTTGTGACAGGCA
TCGGCCGCACCGGTGTCGTCGGCGTGATCCGCGGCCGCGAGCCGTCATCGCGGGTGATCGGACTGCGCGCCGACATGGAC
GCGCTGCCGCTGCAGGAGACCAGGGACATCCCGCACAAGTCGACCATTCCCGGAAGGATGCACGCCTGCGGGCATGACGG
CCACACCGCGATGCTGCTGGGCGCGGCGCGCTACCTCGCGGAGACGCGCAACTTCGCCGGCACCGCCGTCGTGATCTTTC
AGCCGGCCGAGGAGGGCGGGGCCGGCGGCCGCGCGATGGTCGAGGACGGCCTGATGGAGCGCTTCGGCATCGAGGAGGTC
TACGGCCTGCACAATGCGCCGGGCGTGCCTCTCGGCAACGTCTCGACGCGCGTCGGCGCTGTCATGGCGGCCGCCGATAC
GTTCGAGGTGCGCCTCAAGGGCCTCGGCGGTCACGCCGCGCGGCCGAACAAATGCGTCGACCCGATCATCGCCGGCGCGC
AGATCGTGACCGCGCTGCAGACGATCGTGGCGCGCAACGTCGACCCGGTCGAGAGCGCGGTGCTGTCGATCACGCGCTTC
CATGCAGGCACTTCGGCCGACAACATCATCCCGCAGACCGCGGTCATCGGCGGCACCGTGCGCACGCTCGACGAGGAGGT
CCGGCGTCTGATGGACAAGCGCTTCAAGGATCTCGTCACGGCGATGGCCAGCGGCATGGGTGTCGAGGTCGAAATCGATT
ACGAATGGGGCTATCCCGTCGTCATCAACCACGCGGAGCAGACGGCATTCGCGGCCCAGGTCGCGCGCAGCGTGGTCGGG
CCGGACCAGGTCAGCACCGACATGCCGCCGCGGCTCGGCGGCGAGGACTTCGCCTACATGCTGCAGGCGCGGCCCGGCGC
CTTCGTGTTCATGGGCATCGGCGACGGCGCGGGCGTGCATCACCCCGAATACGATTTCAACGACGACGTGATCCCGCACG
GCATCAGCTATTGGGCCAAGCTCGTTGAGACGGCGATGCCGGCCGGCTGA

Upstream 100 bases:

>100_bases
TCCGAACGCTCGAAGGGCCGCGCGATCTCCGCGCGCGACATGGCGTTCAATTCACACCAACAGTCCGAATCGAGCTGCGC
TCGTCACTGGGAATATCGAC

Downstream 100 bases:

>100_bases
ACGCCCGGCGCTCAGATCGCCGCCGCGGCGTCCTCTGTGCGGCGGTCGCTCTTGAAATAGTCGATCATGACCTTGGCGAT
CGCCATCAGCGGCAGCGCCA

Product: putative hippurate hydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MPIINRAAELAEDAKVWRRDFHQHPELQYEVHRTAARVAELLTSFGVDEVVTGIGRTGVVGVIRGREPSSRVIGLRADMD
ALPLQETRDIPHKSTIPGRMHACGHDGHTAMLLGAARYLAETRNFAGTAVVIFQPAEEGGAGGRAMVEDGLMERFGIEEV
YGLHNAPGVPLGNVSTRVGAVMAAADTFEVRLKGLGGHAARPNKCVDPIIAGAQIVTALQTIVARNVDPVESAVLSITRF
HAGTSADNIIPQTAVIGGTVRTLDEEVRRLMDKRFKDLVTAMASGMGVEVEIDYEWGYPVVINHAEQTAFAAQVARSVVG
PDQVSTDMPPRLGGEDFAYMLQARPGAFVFMGIGDGAGVHHPEYDFNDDVIPHGISYWAKLVETAMPAG

Sequences:

>Translated_389_residues
MPIINRAAELAEDAKVWRRDFHQHPELQYEVHRTAARVAELLTSFGVDEVVTGIGRTGVVGVIRGREPSSRVIGLRADMD
ALPLQETRDIPHKSTIPGRMHACGHDGHTAMLLGAARYLAETRNFAGTAVVIFQPAEEGGAGGRAMVEDGLMERFGIEEV
YGLHNAPGVPLGNVSTRVGAVMAAADTFEVRLKGLGGHAARPNKCVDPIIAGAQIVTALQTIVARNVDPVESAVLSITRF
HAGTSADNIIPQTAVIGGTVRTLDEEVRRLMDKRFKDLVTAMASGMGVEVEIDYEWGYPVVINHAEQTAFAAQVARSVVG
PDQVSTDMPPRLGGEDFAYMLQARPGAFVFMGIGDGAGVHHPEYDFNDDVIPHGISYWAKLVETAMPAG
>Mature_388_residues
PIINRAAELAEDAKVWRRDFHQHPELQYEVHRTAARVAELLTSFGVDEVVTGIGRTGVVGVIRGREPSSRVIGLRADMDA
LPLQETRDIPHKSTIPGRMHACGHDGHTAMLLGAARYLAETRNFAGTAVVIFQPAEEGGAGGRAMVEDGLMERFGIEEVY
GLHNAPGVPLGNVSTRVGAVMAAADTFEVRLKGLGGHAARPNKCVDPIIAGAQIVTALQTIVARNVDPVESAVLSITRFH
AGTSADNIIPQTAVIGGTVRTLDEEVRRLMDKRFKDLVTAMASGMGVEVEIDYEWGYPVVINHAEQTAFAAQVARSVVGP
DQVSTDMPPRLGGEDFAYMLQARPGAFVFMGIGDGAGVHHPEYDFNDDVIPHGISYWAKLVETAMPAG

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=414, Percent_Identity=28.9855072463768, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41743; Mature: 41612

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIINRAAELAEDAKVWRRDFHQHPELQYEVHRTAARVAELLTSFGVDEVVTGIGRTGVV
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEE
GVIRGREPSSRVIGLRADMDALPLQETRDIPHKSTIPGRMHACGHDGHTAMLLGAARYLA
EEEECCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHH
ETRNFAGTAVVIFQPAEEGGAGGRAMVEDGLMERFGIEEVYGLHNAPGVPLGNVSTRVGA
HHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCHHHHHH
VMAAADTFEVRLKGLGGHAARPNKCVDPIIAGAQIVTALQTIVARNVDPVESAVLSITRF
HHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
HAGTSADNIIPQTAVIGGTVRTLDEEVRRLMDKRFKDLVTAMASGMGVEVEIDYEWGYPV
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCE
VINHAEQTAFAAQVARSVVGPDQVSTDMPPRLGGEDFAYMLQARPGAFVFMGIGDGAGVH
EEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHEECCCCEEEEEECCCCCCCC
HPEYDFNDDVIPHGISYWAKLVETAMPAG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PIINRAAELAEDAKVWRRDFHQHPELQYEVHRTAARVAELLTSFGVDEVVTGIGRTGVV
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEE
GVIRGREPSSRVIGLRADMDALPLQETRDIPHKSTIPGRMHACGHDGHTAMLLGAARYLA
EEEECCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHH
ETRNFAGTAVVIFQPAEEGGAGGRAMVEDGLMERFGIEEVYGLHNAPGVPLGNVSTRVGA
HHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCHHHHHH
VMAAADTFEVRLKGLGGHAARPNKCVDPIIAGAQIVTALQTIVARNVDPVESAVLSITRF
HHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
HAGTSADNIIPQTAVIGGTVRTLDEEVRRLMDKRFKDLVTAMASGMGVEVEIDYEWGYPV
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCE
VINHAEQTAFAAQVARSVVGPDQVSTDMPPRLGGEDFAYMLQARPGAFVFMGIGDGAGVH
EEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHEECCCCEEEEEECCCCCCCC
HPEYDFNDDVIPHGISYWAKLVETAMPAG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]