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The map label for this gene is puuD [H]

Identifier: 146341138

GI number: 146341138

Start: 4409896

End: 4410666

Strand: Reverse

Name: puuD [H]

Synonym: BRADO4209

Alternate gene names: 146341138

Gene position: 4410666-4409896 (Counterclockwise)

Preceding gene: 146341140

Following gene: 146341137

Centisome position: 59.15

GC content: 66.15

Gene sequence:

>771_bases
ATGAAGCGTCCGGTCGTCGGCGTGATCGGCAACAGCCATCGCGTCGAGAACAGATTTACGGTGCAGATGGTGGGCGAGCG
CAATCTGAGCGCCGTGGCGGAGGTCGCGGGGGCGCTGCCTTTGATGTTTGCCGGCGTCCCGGAGATCACCGACATCGCCG
CGCTGCTCGACGTTGTCGACGGCGTCGTGCTGACCGGCGCTCGCGCCAACGTGCATCCGACCCGGTTCAACACCGAGCCG
AGCGTCAAGCATGAGCCCTACGACATCCACCGCGACGAGGTCGCCCTGCAGCTGTCCGAGGCCTGCGTTGCCCGGGGCAT
TCCGCTGTTCGGGATCTGCCGCGGGCTGCAGGAGATGAACGTCGCCTTCGGCGGCTCGCTGCATCCGGAGATCCGCGAGA
TACCCGGCCGCATGAACCACCGGATGCCCAGGCTGGAGAACGGCGAGATCCATCCCGACCCCAACGTCGTGTTCGCCGAC
CGCCATGAGGTCAAGCTGACACCAGGCGGCGCCTTTGCCTCCATCCTCGGCTGCGACTGCATCAAGGTGAACTCGCTGCA
CGGGCAGGGCATCCTCAATCCCGGCGACCGCGTGATCATCGAGGGCATCGCCGAGGACGGCACCATCGAGGCCATCCGCA
TCGCCGAAGCGCCGAGCTTTGCGCTCGGCGTGCAATGGCACGCCGAATACGACCCGCAGAAGAACCCGATCAACCGCAAA
CTGTTCGAGGCGTTTGGCGAGGCTGTGAGAAGGCGGCGGCTCGCGGCCTAA

Upstream 100 bases:

>100_bases
GACCCGCGCGCTCATCTGTTGATCCGGCTGTACGACACGGGCATGATTTGCGAAAGGCACGATTTGTGCCTTCCGCGGGA
GAGAGACGGAAAAGAGGGTC

Downstream 100 bases:

>100_bases
CGTCGGGCTGAACCTGCCGTGCGGCGAAGCAGACGTAAGGCTGATTGCGTTGTCTCGTCGTCTCACAAAGGTTCATTGAT
GCGCCATCTCCTGACCGTCG

Product: putative gamma-glutamyl hydrolase family protein

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKRPVVGVIGNSHRVENRFTVQMVGERNLSAVAEVAGALPLMFAGVPEITDIAALLDVVDGVVLTGARANVHPTRFNTEP
SVKHEPYDIHRDEVALQLSEACVARGIPLFGICRGLQEMNVAFGGSLHPEIREIPGRMNHRMPRLENGEIHPDPNVVFAD
RHEVKLTPGGAFASILGCDCIKVNSLHGQGILNPGDRVIIEGIAEDGTIEAIRIAEAPSFALGVQWHAEYDPQKNPINRK
LFEAFGEAVRRRRLAA

Sequences:

>Translated_256_residues
MKRPVVGVIGNSHRVENRFTVQMVGERNLSAVAEVAGALPLMFAGVPEITDIAALLDVVDGVVLTGARANVHPTRFNTEP
SVKHEPYDIHRDEVALQLSEACVARGIPLFGICRGLQEMNVAFGGSLHPEIREIPGRMNHRMPRLENGEIHPDPNVVFAD
RHEVKLTPGGAFASILGCDCIKVNSLHGQGILNPGDRVIIEGIAEDGTIEAIRIAEAPSFALGVQWHAEYDPQKNPINRK
LFEAFGEAVRRRRLAA
>Mature_256_residues
MKRPVVGVIGNSHRVENRFTVQMVGERNLSAVAEVAGALPLMFAGVPEITDIAALLDVVDGVVLTGARANVHPTRFNTEP
SVKHEPYDIHRDEVALQLSEACVARGIPLFGICRGLQEMNVAFGGSLHPEIREIPGRMNHRMPRLENGEIHPDPNVVFAD
RHEVKLTPGGAFASILGCDCIKVNSLHGQGILNPGDRVIIEGIAEDGTIEAIRIAEAPSFALGVQWHAEYDPQKNPINRK
LFEAFGEAVRRRRLAA

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=255, Percent_Identity=36.4705882352941, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 27903; Mature: 27903

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPVVGVIGNSHRVENRFTVQMVGERNLSAVAEVAGALPLMFAGVPEITDIAALLDVVD
CCCCEEEECCCCEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
GVVLTGARANVHPTRFNTEPSVKHEPYDIHRDEVALQLSEACVARGIPLFGICRGLQEMN
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VAFGGSLHPEIREIPGRMNHRMPRLENGEIHPDPNVVFADRHEVKLTPGGAFASILGCDC
HHCCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEECCCEEEECCCCHHHHHHCCCE
IKVNSLHGQGILNPGDRVIIEGIAEDGTIEAIRIAEAPSFALGVQWHAEYDPQKNPINRK
EEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHH
LFEAFGEAVRRRRLAA
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRPVVGVIGNSHRVENRFTVQMVGERNLSAVAEVAGALPLMFAGVPEITDIAALLDVVD
CCCCEEEECCCCEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
GVVLTGARANVHPTRFNTEPSVKHEPYDIHRDEVALQLSEACVARGIPLFGICRGLQEMN
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VAFGGSLHPEIREIPGRMNHRMPRLENGEIHPDPNVVFADRHEVKLTPGGAFASILGCDC
HHCCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEECCCEEEECCCCHHHHHHCCCE
IKVNSLHGQGILNPGDRVIIEGIAEDGTIEAIRIAEAPSFALGVQWHAEYDPQKNPINRK
EEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHH
LFEAFGEAVRRRRLAA
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]