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The map label for this gene is glnA [H]

Identifier: 146341134

GI number: 146341134

Start: 4407019

End: 4408428

Strand: Direct

Name: glnA [H]

Synonym: BRADO4205

Alternate gene names: 146341134

Gene position: 4407019-4408428 (Clockwise)

Preceding gene: 146341133

Following gene: 146341139

Centisome position: 59.1

GC content: 62.98

Gene sequence:

>1410_bases
ATGAAGACCGCCAAGGACGTCTTGAAAGCAATCAAAGACAACGACGTCAAATACGTCGACCTCCGCTTCACCGACCCGCG
GGGCAAGTGGCAGCACGTCACCTTCGACGTCAGCATGATCGATGAAGACATTTTCGCTGAAGGCACCATGTTCGACGGCT
CCTCGATCGCCGGCTGGAAGGCGATCAACGAATCCGACATGTGCCTGATGCCCGACCCGGTGACCGCGACGATCGATCCG
TTCTTCGCCGAAACCACCATGATCATCACCTGCGACGTGCTCGAGCCGACCACCGGCGAGCCGTACAACCGCGACCCCCG
CGGCATCGCCAAGAAGGCCGAGGCGATGGTGAAGTCGATGGGCGTGGGCGATTCCGTGTTCGTCGGCCCCGAGGCCGAGT
TTTTCGTGTTCGACGACGTGCGCTACAGCTCCTCGCCGTACAACACCGGCTTCCGCCTCGACTCCTCGGAGCTGCCGACC
AACTCCGATACCGAGTATGAGGGCGGCAACCTCGGCCACCGCATCCGCACCAAGGCCGGCTACTTCCCGGTGCCGCCGCA
GGACTCGGTGCAGGACATGCGCTCGGAGATGCTCGGCGCAATGGCTCGCATGGGCGTCAAGGTCGAGAAGCACCATCACG
AGGTCGCCTCCGCCCAGCACGAGCTCGGCATGAAGTTCGACACGCTGACCCTGATGGCCGACCAGATGCAGATCTACAAG
TACTGCATCCAGCAGGTCGCGCACATCTACGGCAAGACCGCCACCTTCATGCCGAAGCCGGTGTACGGCGACAACGGCTC
GGGCATGCACGTCCACCAGTCGATCTGGAAGGACGGCAAGCCGGTGTTCGCGGGCAACAAATATTCCGACCTGTCGGAGA
CCTGCCTGTCCTACATCGCCGGCATCATCAAGCACGCCAAGGCCATCAACGCCTTCACCAACCCGTCGACCAACTCCTAC
AAGCGTCTGGTGCCGGGCTATGAGGCGCCGGTGCTGCTCGCCTACTCCGCGCGCAACCGCTCGGCCTCGTGCCGCATCCC
CTACACCTCCTCGCCGAAGGCCAAGCGCGTCGAGGTCCGCTTCCCCGATCCGCTCGCCAACCCGTATCTCGGCTTCGCCG
CGATGCTGATGGCCGGCCTCGACGGCATCAAGAACAAGATGGATCCGGGTCCGGCGATGGACAAGGACCTCTACGACCTG
CCGAAGGAGGAGCTGAAGCAGATCCCGACCGTTTGCGGTTCGCTCCGCGAGGCGCTCGAGAACCTCGACAAGGACCGCGC
CTTCCTGAAGGCCGGCGGCGTGTTCGACGACGACTTCATCGACAGCTACATCGAACTGAAGATGACCGAGGTCGCGCGCT
TCGAAATGACCCCGCACCCGGTCGAGTTCGACATGTACTACTCGGGCTGA

Upstream 100 bases:

>100_bases
CGTTTCCGATGTTGTCTTGGCCCCCGGGCGAGGCTATGGAGCATGACCCGCGGACGCCGTCCCGCGCCGGGCCGTGACGT
TTCAGCCAAGAGGGGTATTC

Downstream 100 bases:

>100_bases
CAAGCCCAATTGATCGGCGGATCACGAAGGCGCCCCTCGGGGCGCCTTTTTGTTTGCCGGGGCGAACTGTCCGGTGGCGA
GAGGTCACGGGCTCTCGAGA

Product: glutamine synthetase I

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKTAKDVLKAIKDNDVKYVDLRFTDPRGKWQHVTFDVSMIDEDIFAEGTMFDGSSIAGWKAINESDMCLMPDPVTATIDP
FFAETTMIITCDVLEPTTGEPYNRDPRGIAKKAEAMVKSMGVGDSVFVGPEAEFFVFDDVRYSSSPYNTGFRLDSSELPT
NSDTEYEGGNLGHRIRTKAGYFPVPPQDSVQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTLMADQMQIYK
YCIQQVAHIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPVFAGNKYSDLSETCLSYIAGIIKHAKAINAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPYTSSPKAKRVEVRFPDPLANPYLGFAAMLMAGLDGIKNKMDPGPAMDKDLYDL
PKEELKQIPTVCGSLREALENLDKDRAFLKAGGVFDDDFIDSYIELKMTEVARFEMTPHPVEFDMYYSG

Sequences:

>Translated_469_residues
MKTAKDVLKAIKDNDVKYVDLRFTDPRGKWQHVTFDVSMIDEDIFAEGTMFDGSSIAGWKAINESDMCLMPDPVTATIDP
FFAETTMIITCDVLEPTTGEPYNRDPRGIAKKAEAMVKSMGVGDSVFVGPEAEFFVFDDVRYSSSPYNTGFRLDSSELPT
NSDTEYEGGNLGHRIRTKAGYFPVPPQDSVQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTLMADQMQIYK
YCIQQVAHIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPVFAGNKYSDLSETCLSYIAGIIKHAKAINAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPYTSSPKAKRVEVRFPDPLANPYLGFAAMLMAGLDGIKNKMDPGPAMDKDLYDL
PKEELKQIPTVCGSLREALENLDKDRAFLKAGGVFDDDFIDSYIELKMTEVARFEMTPHPVEFDMYYSG
>Mature_469_residues
MKTAKDVLKAIKDNDVKYVDLRFTDPRGKWQHVTFDVSMIDEDIFAEGTMFDGSSIAGWKAINESDMCLMPDPVTATIDP
FFAETTMIITCDVLEPTTGEPYNRDPRGIAKKAEAMVKSMGVGDSVFVGPEAEFFVFDDVRYSSSPYNTGFRLDSSELPT
NSDTEYEGGNLGHRIRTKAGYFPVPPQDSVQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTLMADQMQIYK
YCIQQVAHIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPVFAGNKYSDLSETCLSYIAGIIKHAKAINAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPYTSSPKAKRVEVRFPDPLANPYLGFAAMLMAGLDGIKNKMDPGPAMDKDLYDL
PKEELKQIPTVCGSLREALENLDKDRAFLKAGGVFDDDFIDSYIELKMTEVARFEMTPHPVEFDMYYSG

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=486, Percent_Identity=22.8395061728395, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1790301, Length=467, Percent_Identity=64.2398286937901, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=397, Percent_Identity=25.1889168765743, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52313; Mature: 52313

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAKDVLKAIKDNDVKYVDLRFTDPRGKWQHVTFDVSMIDEDIFAEGTMFDGSSIAGWK
CCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHCCCCEECCCCCCCCE
AINESDMCLMPDPVTATIDPFFAETTMIITCDVLEPTTGEPYNRDPRGIAKKAEAMVKSM
ECCCCCCEECCCCCCEECCCHHCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHC
GVGDSVFVGPEAEFFVFDDVRYSSSPYNTGFRLDSSELPTNSDTEYEGGNLGHRIRTKAG
CCCCCEEECCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEECCCC
YFPVPPQDSVQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTLMADQMQIYK
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YCIQQVAHIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPVFAGNKYSDLSETCLSYIA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEECCCCCCHHHHHHHHHHH
GIIKHAKAINAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYTSSPKAKRVEVR
HHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPLANPYLGFAAMLMAGLDGIKNKMDPGPAMDKDLYDLPKEELKQIPTVCGSLREALE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
NLDKDRAFLKAGGVFDDDFIDSYIELKMTEVARFEMTPHPVEFDMYYSG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEEEEECC
>Mature Secondary Structure
MKTAKDVLKAIKDNDVKYVDLRFTDPRGKWQHVTFDVSMIDEDIFAEGTMFDGSSIAGWK
CCCHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEHHHHHHHHHCCCCEECCCCCCCCE
AINESDMCLMPDPVTATIDPFFAETTMIITCDVLEPTTGEPYNRDPRGIAKKAEAMVKSM
ECCCCCCEECCCCCCEECCCHHCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHC
GVGDSVFVGPEAEFFVFDDVRYSSSPYNTGFRLDSSELPTNSDTEYEGGNLGHRIRTKAG
CCCCCEEECCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEECCCC
YFPVPPQDSVQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTLMADQMQIYK
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YCIQQVAHIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPVFAGNKYSDLSETCLSYIA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEECCCCCCHHHHHHHHHHH
GIIKHAKAINAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYTSSPKAKRVEVR
HHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPLANPYLGFAAMLMAGLDGIKNKMDPGPAMDKDLYDLPKEELKQIPTVCGSLREALE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
NLDKDRAFLKAGGVFDDDFIDSYIELKMTEVARFEMTPHPVEFDMYYSG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275; 2859270; 2793830 [H]