Click here to switch to the map view.

The map label for this gene is mdlA [H]

Identifier: 146341131

GI number: 146341131

Start: 4403147

End: 4404229

Strand: Direct

Name: mdlA [H]

Synonym: BRADO4202

Alternate gene names: 146341131

Gene position: 4403147-4404229 (Clockwise)

Preceding gene: 146341130

Following gene: 146341133

Centisome position: 59.05

GC content: 68.14

Gene sequence:

>1083_bases
ATGACCACCGCCGCCATCACCGGCGTCACCGCGCGCGCCGTCATCACGCCGATGAAGCGCCCGCTGCGCAACGCCTTCGG
CGTGATCGATTCAGGCCCGCTGGTGCTGATCGACGTCACGACAGATCAGGGCGTGACCGGCCATTCCTATCTCTTCGCCT
ACACCAGGCTGGCTCTGAAGCCGCTCGTGCATCTGGTCGAGGATATCGGCCGCGAGCTCGCCGGCAAGGCGCTGGTGCCG
GTCGATCTGATGAAGGCGATGGACGCGAAATTCCGGCTGCTCGGCTGGCAGGGGCTGGTCGGCATGGCCGTCTCGGGGCT
CGACATGGCGTTCTGGGATGCGCTCGGCCAGCTCGCGGGCAAGCCCGTCGTCGAGCTGCTCGGCGGCAGCGCTCGTCCCA
TTCCCGCCTATGACAGCTACGGCGTGCTCGACGCCCGGGACGACGAGCGCACCTTGCGCACCGCGTGCGACGAGCACGGC
TTCCGCGCCATCAAGAGCAAGGGCGGCCATGGCGACCTCGCCACCGACGAGGCGATGATCAAGGGCCTGCGCGCTTTGCT
CGGGCCCGACATCGCGCTGATGCTCGACTTCAACCAGTCGCTCGATCCCGCTGAGGCCACGCGCAGAATCGCGCGTCTTG
CGGATTACGACCTGACCTGGATCGAGGAGCCGGTGCCGCAGGAGAATCTCTCCGGCCACGCCGCGGTGCGCGAGCGCTCG
GAGATCCCGATCCAGGCCGGCGAGAACTGGTGGTTTCCGCGCGGCTTCGCCGAAGCGATCGCCGCCGGCGCCTCCGACTT
CATCATGCCCGACCTGATGAAGGTCGGCGGCATCACCGGCTGGCTCAATGTCGCCGGCCAGGCCGACGCCGCCTCGATCC
CGATGTCGAGCCACATCCTGCCCGAGGCCAGCGCCCATGTGCTGCCGGTGACGCCGACGGCGCATTTCCTCGAAGTGCTC
GACTTCGCCGGGGCCATTCTCACCGAGCCGCTGCGGGTGATCGACGGCAAGGTGACCGCGAAGGGCCCGGGTCTCGGACT
TGCGTGGAACGAGAGCGCGGTGGCGAAGTATCAGGTGACGTGA

Upstream 100 bases:

>100_bases
TGGCCGGTTCCTGGTCGAGAGCATGGCCACCGACTGGGCGGCGCCACGCCTCGTCAGGTGGTATGAGATCGGGTGAGGAA
ACGAACCAGGGAGAACTCCC

Downstream 100 bases:

>100_bases
GCCATACTGGCGTCCGCGAGACGCATCGACGCCGCCGCAAACTCAGCTGGATCGCCCGGCTTGACCGGGCGATCCAGTAT
GCCGAGGCGTCTTTGATTGA

Product: putative mandelate racemase/muconate lactonizing family protein

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTTAAITGVTARAVITPMKRPLRNAFGVIDSGPLVLIDVTTDQGVTGHSYLFAYTRLALKPLVHLVEDIGRELAGKALVP
VDLMKAMDAKFRLLGWQGLVGMAVSGLDMAFWDALGQLAGKPVVELLGGSARPIPAYDSYGVLDARDDERTLRTACDEHG
FRAIKSKGGHGDLATDEAMIKGLRALLGPDIALMLDFNQSLDPAEATRRIARLADYDLTWIEEPVPQENLSGHAAVRERS
EIPIQAGENWWFPRGFAEAIAAGASDFIMPDLMKVGGITGWLNVAGQADAASIPMSSHILPEASAHVLPVTPTAHFLEVL
DFAGAILTEPLRVIDGKVTAKGPGLGLAWNESAVAKYQVT

Sequences:

>Translated_360_residues
MTTAAITGVTARAVITPMKRPLRNAFGVIDSGPLVLIDVTTDQGVTGHSYLFAYTRLALKPLVHLVEDIGRELAGKALVP
VDLMKAMDAKFRLLGWQGLVGMAVSGLDMAFWDALGQLAGKPVVELLGGSARPIPAYDSYGVLDARDDERTLRTACDEHG
FRAIKSKGGHGDLATDEAMIKGLRALLGPDIALMLDFNQSLDPAEATRRIARLADYDLTWIEEPVPQENLSGHAAVRERS
EIPIQAGENWWFPRGFAEAIAAGASDFIMPDLMKVGGITGWLNVAGQADAASIPMSSHILPEASAHVLPVTPTAHFLEVL
DFAGAILTEPLRVIDGKVTAKGPGLGLAWNESAVAKYQVT
>Mature_359_residues
TTAAITGVTARAVITPMKRPLRNAFGVIDSGPLVLIDVTTDQGVTGHSYLFAYTRLALKPLVHLVEDIGRELAGKALVPV
DLMKAMDAKFRLLGWQGLVGMAVSGLDMAFWDALGQLAGKPVVELLGGSARPIPAYDSYGVLDARDDERTLRTACDEHGF
RAIKSKGGHGDLATDEAMIKGLRALLGPDIALMLDFNQSLDPAEATRRIARLADYDLTWIEEPVPQENLSGHAAVRERSE
IPIQAGENWWFPRGFAEAIAAGASDFIMPDLMKVGGITGWLNVAGQADAASIPMSSHILPEASAHVLPVTPTAHFLEVLD
FAGAILTEPLRVIDGKVTAKGPGLGLAWNESAVAKYQVT

Specific function: Unknown

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=334, Percent_Identity=25.1497005988024, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI186972148, Length=248, Percent_Identity=27.4193548387097, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI226510960, Length=372, Percent_Identity=26.0752688172043, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994953, Length=355, Percent_Identity=27.3239436619718, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787864, Length=300, Percent_Identity=25.3333333333333, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 38436; Mature: 38304

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAAITGVTARAVITPMKRPLRNAFGVIDSGPLVLIDVTTDQGVTGHSYLFAYTRLALK
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
PLVHLVEDIGRELAGKALVPVDLMKAMDAKFRLLGWQGLVGMAVSGLDMAFWDALGQLAG
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHCC
KPVVELLGGSARPIPAYDSYGVLDARDDERTLRTACDEHGFRAIKSKGGHGDLATDEAMI
CHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
KGLRALLGPDIALMLDFNQSLDPAEATRRIARLADYDLTWIEEPVPQENLSGHAAVRERS
HHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHHCC
EIPIQAGENWWFPRGFAEAIAAGASDFIMPDLMKVGGITGWLNVAGQADAASIPMSSHIL
CCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEHCCCCCCCCCCCCCCCCCC
PEASAHVLPVTPTAHFLEVLDFAGAILTEPLRVIDGKVTAKGPGLGLAWNESAVAKYQVT
CCCCCCEEECCCHHHHHHHHHHHHHHHHCCHHHHCCEEEECCCCCCEEECCCCCEEEECC
>Mature Secondary Structure 
TTAAITGVTARAVITPMKRPLRNAFGVIDSGPLVLIDVTTDQGVTGHSYLFAYTRLALK
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
PLVHLVEDIGRELAGKALVPVDLMKAMDAKFRLLGWQGLVGMAVSGLDMAFWDALGQLAG
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHCC
KPVVELLGGSARPIPAYDSYGVLDARDDERTLRTACDEHGFRAIKSKGGHGDLATDEAMI
CHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
KGLRALLGPDIALMLDFNQSLDPAEATRRIARLADYDLTWIEEPVPQENLSGHAAVRERS
HHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHHCC
EIPIQAGENWWFPRGFAEAIAAGASDFIMPDLMKVGGITGWLNVAGQADAASIPMSSHIL
CCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEHCCCCCCCCCCCCCCCCCC
PEASAHVLPVTPTAHFLEVLDFAGAILTEPLRVIDGKVTAKGPGLGLAWNESAVAKYQVT
CCCCCCEEECCCHHHHHHHHHHHHHHHHCCHHHHCCEEEECCCCCCEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]