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The map label for this gene is pepT [H]

Identifier: 146341118

GI number: 146341118

Start: 4389412

End: 4390668

Strand: Direct

Name: pepT [H]

Synonym: BRADO4189

Alternate gene names: 146341118

Gene position: 4389412-4390668 (Clockwise)

Preceding gene: 146341117

Following gene: 146341121

Centisome position: 58.87

GC content: 64.12

Gene sequence:

>1257_bases
ATGTCAGACCTGCTGCCCAGCTATACGCACTCCGTGACTGAGCGCTTTCTTCGCTACGTCGTGATCGATACCCAGTCTGA
CCCGACGTCCCCCACCTGTCCCTCCACCCTCAAGCAGAAGAACCTAGGGCGGCTGCTTGCCGCCGAACTGCAAGCCATGG
GCTTGCCGGACGCGCATATGGACGAGCACGGCTATGTCTATGCGACGATCCCAGCGACGACCGCGAAAGCCGTTCCGGTC
ATCTGCTTCTGTTCGCACATGGACACCTCGCCTGACTGCTCCGGGACCGACGTGAAGCCGCAGATCGTGCGCAACTATCA
GGGCGGCGACATCGTGCTGCCTGGTGATCCGAGCCAGGTGATCCGCCCGGCCGAGCATCCGGCTCTGGCCGACCAGATCG
GCTGCGACATCGTCACATCTGACGGCACCACCCTGCTGGGCGCCGATAACAAGGCCGGCATCGCCGAGATCATGGACGCG
GCGCAGTTCCTCGTGAATCATCCAGAAATCAAGCACGGCACGATCAAGATCCTGTTCACCCCCGACGAGGAGATCGGGCG
CGGCGTCGACAAGGTCGACCTGCAGAAGCTCGGCGCCGATTTCGGCTACACCATGGACGGCGAGACCGCGGGCAATATCG
AGGACGAGACCTTCTCGGCGGACGGCGCGACGATCATCATCAATGGCGTCAGCGCGCATCCCGGCTTTGCCAAGGGCAAG
ATGGAGCACGCCATCAAGATCGCCTCGGCGATCATCGATCGCCTGCCGAAGGACACCTGCTCGCCGGAGACGACCGAGGG
CAAGGAAGGCTTCCTGCATCCGGTCGGCATCTCCGGCGCGCTCGAAGAGGCCCGGCTGGACTTCATCGTCCGCGACTTCA
GCGAGCAGGGCCTGCGCCAGAAGGAGGCGCTGCTCGAGGACATCGTCAAGGAGGTGATGCAGGACTACCCGCGCTCCAGC
TACGAGATGCAGGTCAAGACGCAGTACCGCAACATGAAGGAGGTCATCGACCACCATCCGGAGATCGTGACCTACGCCGA
GGACGCCATCCGCCGCGCGGGGCTGACGCCGGTGCGGACCTCGATCCGCGGCGGCACCGACGGCTCGCGACTGTCCTTCA
TGGGGCTGCCCTGCCCCAACATCTTCGCCGGCGAGCACGCCTTCCACTCGCGCCTGGAATGGGTCAGCGTCCGTGACATG
GAGAAGGCGGTGCAGACCATCGTGCATCTCGCGATGATCTGGGAGGAGCGCTCCTGA

Upstream 100 bases:

>100_bases
CTAAGGCTTGCGCTAACCTTGGCGTGTGGCATGCCAATCTGATATACTAGTATATGTCAGACGTATGGTAATATAATCCT
CCATTCTCCTCGGGGTGCTC

Downstream 100 bases:

>100_bases
CACCGGTCCGGCGCGGGCCTCAGGCGCGCGCCGTCACCAGCCAGATCGAGCCGGGCAGCGTGACGCTGCCGCCATTCTGA
TATTGCGCCAGCAGTTCGCG

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MSDLLPSYTHSVTERFLRYVVIDTQSDPTSPTCPSTLKQKNLGRLLAAELQAMGLPDAHMDEHGYVYATIPATTAKAVPV
ICFCSHMDTSPDCSGTDVKPQIVRNYQGGDIVLPGDPSQVIRPAEHPALADQIGCDIVTSDGTTLLGADNKAGIAEIMDA
AQFLVNHPEIKHGTIKILFTPDEEIGRGVDKVDLQKLGADFGYTMDGETAGNIEDETFSADGATIIINGVSAHPGFAKGK
MEHAIKIASAIIDRLPKDTCSPETTEGKEGFLHPVGISGALEEARLDFIVRDFSEQGLRQKEALLEDIVKEVMQDYPRSS
YEMQVKTQYRNMKEVIDHHPEIVTYAEDAIRRAGLTPVRTSIRGGTDGSRLSFMGLPCPNIFAGEHAFHSRLEWVSVRDM
EKAVQTIVHLAMIWEERS

Sequences:

>Translated_418_residues
MSDLLPSYTHSVTERFLRYVVIDTQSDPTSPTCPSTLKQKNLGRLLAAELQAMGLPDAHMDEHGYVYATIPATTAKAVPV
ICFCSHMDTSPDCSGTDVKPQIVRNYQGGDIVLPGDPSQVIRPAEHPALADQIGCDIVTSDGTTLLGADNKAGIAEIMDA
AQFLVNHPEIKHGTIKILFTPDEEIGRGVDKVDLQKLGADFGYTMDGETAGNIEDETFSADGATIIINGVSAHPGFAKGK
MEHAIKIASAIIDRLPKDTCSPETTEGKEGFLHPVGISGALEEARLDFIVRDFSEQGLRQKEALLEDIVKEVMQDYPRSS
YEMQVKTQYRNMKEVIDHHPEIVTYAEDAIRRAGLTPVRTSIRGGTDGSRLSFMGLPCPNIFAGEHAFHSRLEWVSVRDM
EKAVQTIVHLAMIWEERS
>Mature_417_residues
SDLLPSYTHSVTERFLRYVVIDTQSDPTSPTCPSTLKQKNLGRLLAAELQAMGLPDAHMDEHGYVYATIPATTAKAVPVI
CFCSHMDTSPDCSGTDVKPQIVRNYQGGDIVLPGDPSQVIRPAEHPALADQIGCDIVTSDGTTLLGADNKAGIAEIMDAA
QFLVNHPEIKHGTIKILFTPDEEIGRGVDKVDLQKLGADFGYTMDGETAGNIEDETFSADGATIIINGVSAHPGFAKGKM
EHAIKIASAIIDRLPKDTCSPETTEGKEGFLHPVGISGALEEARLDFIVRDFSEQGLRQKEALLEDIVKEVMQDYPRSSY
EMQVKTQYRNMKEVIDHHPEIVTYAEDAIRRAGLTPVRTSIRGGTDGSRLSFMGLPCPNIFAGEHAFHSRLEWVSVRDME
KAVQTIVHLAMIWEERS

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=409, Percent_Identity=44.0097799511002, Blast_Score=339, Evalue=2e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 45760; Mature: 45629

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLPSYTHSVTERFLRYVVIDTQSDPTSPTCPSTLKQKNLGRLLAAELQAMGLPDAHM
CCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
DEHGYVYATIPATTAKAVPVICFCSHMDTSPDCSGTDVKPQIVRNYQGGDIVLPGDPSQV
CCCCEEEEEECCCCCHHCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCHHHH
IRPAEHPALADQIGCDIVTSDGTTLLGADNKAGIAEIMDAAQFLVNHPEIKHGTIKILFT
CCCCCCCCHHHHHCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEC
PDEEIGRGVDKVDLQKLGADFGYTMDGETAGNIEDETFSADGATIIINGVSAHPGFAKGK
CCHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCCCCCCCHHH
MEHAIKIASAIIDRLPKDTCSPETTEGKEGFLHPVGISGALEEARLDFIVRDFSEQGLRQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHH
KEALLEDIVKEVMQDYPRSSYEMQVKTQYRNMKEVIDHHPEIVTYAEDAIRRAGLTPVRT
HHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCHHHH
SIRGGTDGSRLSFMGLPCPNIFAGEHAFHSRLEWVSVRDMEKAVQTIVHLAMIWEERS
HCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDLLPSYTHSVTERFLRYVVIDTQSDPTSPTCPSTLKQKNLGRLLAAELQAMGLPDAHM
CCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
DEHGYVYATIPATTAKAVPVICFCSHMDTSPDCSGTDVKPQIVRNYQGGDIVLPGDPSQV
CCCCEEEEEECCCCCHHCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCHHHH
IRPAEHPALADQIGCDIVTSDGTTLLGADNKAGIAEIMDAAQFLVNHPEIKHGTIKILFT
CCCCCCCCHHHHHCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEC
PDEEIGRGVDKVDLQKLGADFGYTMDGETAGNIEDETFSADGATIIINGVSAHPGFAKGK
CCHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCCCCCCCHHH
MEHAIKIASAIIDRLPKDTCSPETTEGKEGFLHPVGISGALEEARLDFIVRDFSEQGLRQ
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHH
KEALLEDIVKEVMQDYPRSSYEMQVKTQYRNMKEVIDHHPEIVTYAEDAIRRAGLTPVRT
HHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCHHHH
SIRGGTDGSRLSFMGLPCPNIFAGEHAFHSRLEWVSVRDMEKAVQTIVHLAMIWEERS
HCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA