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The map label for this gene is gltA [H]

Identifier: 146341055

GI number: 146341055

Start: 4320458

End: 4321762

Strand: Direct

Name: gltA [H]

Synonym: BRADO4126

Alternate gene names: 146341055

Gene position: 4320458-4321762 (Clockwise)

Preceding gene: 146341054

Following gene: 146341077

Centisome position: 57.94

GC content: 62.07

Gene sequence:

>1305_bases
ATGGACGCAAAATCCGAAAGCAAGACCGCGACGCTCACCGTCGGCAACAAGAACTACGATCTTCCGATCCTGAGCGGCAC
CGTGGGTCCCGACGTCATCGACATCGGCAAGCTCTACGCCCAGGCCGGGATGTTCACCTATGACCCCGGCTTCACCTCGA
CCGGCAGCTGCCAGTCCAAGATCACCTATATCGACGGCGACGCCGGCGTCCTGGAATACCGCGGCTATCCGATCGAGCAG
CTCGCCGAAAAGGGCGACTTCCTCGAGACCTGCTATCTGCTGCTGTACGGGGAACTCCCGACGCCGGCGCAGAAGAAGGA
TTTCGACTATCGCGTGACCCATCATACGATGGTGCACGAGCAGATGGCCCGCTTCTTCCAGGGCTTCCGCCGCGACGCCC
ATCCGATGGCCGTCATGGTCGCCTCGGTCGGCGCGCTCGCCGCGTTCTATCACGACTCGACCGACATCAACGATCCCAAG
CAGCGGATGATCGCCTCGATCCGGATGATCGCGAAGATCCCGACCCTGGCTGCGATGGCCTACAAATATTCGATCGGCCA
GCCTTTCGTTTATCCGAAGAACGCGCTGTCCTTCTCCGAGAACTTCCTCAACATGTGCTTCGCGGTGCCGTGCGAGGAGT
ACAAGCCAAACCCGGTGCTCGCCAACGCGCTCGACAAGATCTTCATCCTGCACGCCGACCACGAGCAGAACGCCTCGACC
TCCACGGTGCGCATCGCCGGCTCCTCGGGCGCCAACCCGTTCGCCTGCATCGCCGCCGGCATCGCCTGCCTGTGGGGACC
GGCGCATGGCGGCGCCAACGAGGCGGCGCTGAACATGCTGGCCGAGATCGGCACCGTCGACAAGATTCCCGAGTTCATCG
CCAAGGTGAAGGACAAGAACAGCGACGTGCGCCTGATGGGCTTCGGCCATCGCGTCTACAAGAACTATGACCCGCGCGCC
AAGATCATGCAGAAGATGTGTCACGCGGTGCTCAAGGAGACGGGCCACGCCGACGATCAGATGCTCAAGGTCGCGATGGA
GCTCGAGAAGATCGCGCTCAGCGATCAGTACTTCATCGACCGCAAGCTCTACCCGAACGTCGACTTCTACTCGGGCATCA
CCTTGAAGGCGATGGGCTTCCCGGTCTCGATGTTCACCGTGCTGTTCGCGGTCGCCCGCACCGTCGGCTGGATCAGCCAA
TGGAGCGAGATGATCGAGGACCCGCAGCAGAAGATCGGCCGTCCACGCCAGCTCTACACCGGCGTCACCACGCGCCAGTA
CATCGATATCGACAAGCGCAGCTGA

Upstream 100 bases:

>100_bases
GCCACCGCACTAGATAAGGTCCACCCGCCCTGCGATCTCTCGCACATGACAAGGGCCGAAGCCTAACGATCACGCCTAAC
GATCTCATCGGGGACTAACG

Downstream 100 bases:

>100_bases
TCGCGGCGAAGCGATCAATGATGAAAGGCCCGTTCAGCACGCTGAACGGGCCTTTTTTCTTTGCGCTGGCATGTCGGTCA
TCTCAGATCGGATGCGACTT

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MDAKSESKTATLTVGNKNYDLPILSGTVGPDVIDIGKLYAQAGMFTYDPGFTSTGSCQSKITYIDGDAGVLEYRGYPIEQ
LAEKGDFLETCYLLLYGELPTPAQKKDFDYRVTHHTMVHEQMARFFQGFRRDAHPMAVMVASVGALAAFYHDSTDINDPK
QRMIASIRMIAKIPTLAAMAYKYSIGQPFVYPKNALSFSENFLNMCFAVPCEEYKPNPVLANALDKIFILHADHEQNAST
STVRIAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLAEIGTVDKIPEFIAKVKDKNSDVRLMGFGHRVYKNYDPRA
KIMQKMCHAVLKETGHADDQMLKVAMELEKIALSDQYFIDRKLYPNVDFYSGITLKAMGFPVSMFTVLFAVARTVGWISQ
WSEMIEDPQQKIGRPRQLYTGVTTRQYIDIDKRS

Sequences:

>Translated_434_residues
MDAKSESKTATLTVGNKNYDLPILSGTVGPDVIDIGKLYAQAGMFTYDPGFTSTGSCQSKITYIDGDAGVLEYRGYPIEQ
LAEKGDFLETCYLLLYGELPTPAQKKDFDYRVTHHTMVHEQMARFFQGFRRDAHPMAVMVASVGALAAFYHDSTDINDPK
QRMIASIRMIAKIPTLAAMAYKYSIGQPFVYPKNALSFSENFLNMCFAVPCEEYKPNPVLANALDKIFILHADHEQNAST
STVRIAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLAEIGTVDKIPEFIAKVKDKNSDVRLMGFGHRVYKNYDPRA
KIMQKMCHAVLKETGHADDQMLKVAMELEKIALSDQYFIDRKLYPNVDFYSGITLKAMGFPVSMFTVLFAVARTVGWISQ
WSEMIEDPQQKIGRPRQLYTGVTTRQYIDIDKRS
>Mature_434_residues
MDAKSESKTATLTVGNKNYDLPILSGTVGPDVIDIGKLYAQAGMFTYDPGFTSTGSCQSKITYIDGDAGVLEYRGYPIEQ
LAEKGDFLETCYLLLYGELPTPAQKKDFDYRVTHHTMVHEQMARFFQGFRRDAHPMAVMVASVGALAAFYHDSTDINDPK
QRMIASIRMIAKIPTLAAMAYKYSIGQPFVYPKNALSFSENFLNMCFAVPCEEYKPNPVLANALDKIFILHADHEQNAST
STVRIAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLAEIGTVDKIPEFIAKVKDKNSDVRLMGFGHRVYKNYDPRA
KIMQKMCHAVLKETGHADDQMLKVAMELEKIALSDQYFIDRKLYPNVDFYSGITLKAMGFPVSMFTVLFAVARTVGWISQ
WSEMIEDPQQKIGRPRQLYTGVTTRQYIDIDKRS

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=396, Percent_Identity=27.5252525252525, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786939, Length=425, Percent_Identity=66.1176470588235, Blast_Score=593, Evalue=1e-170,
Organism=Escherichia coli, GI1786527, Length=364, Percent_Identity=30.4945054945055, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17555174, Length=397, Percent_Identity=26.1964735516373, Blast_Score=111, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324328, Length=360, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319850, Length=331, Percent_Identity=26.2839879154079, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325257, Length=405, Percent_Identity=24.9382716049383, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24640126, Length=413, Percent_Identity=25.4237288135593, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24640124, Length=413, Percent_Identity=25.4237288135593, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21356863, Length=363, Percent_Identity=26.7217630853994, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48258; Mature: 48258

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAKSESKTATLTVGNKNYDLPILSGTVGPDVIDIGKLYAQAGMFTYDPGFTSTGSCQSK
CCCCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCE
ITYIDGDAGVLEYRGYPIEQLAEKGDFLETCYLLLYGELPTPAQKKDFDYRVTHHTMVHE
EEEEECCCCEEEECCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHH
QMARFFQGFRRDAHPMAVMVASVGALAAFYHDSTDINDPKQRMIASIRMIAKIPTLAAMA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YKYSIGQPFVYPKNALSFSENFLNMCFAVPCEEYKPNPVLANALDKIFILHADHEQNAST
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHCEEEEEECCCCCCCCC
STVRIAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLAEIGTVDKIPEFIAKVKDKN
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCC
SDVRLMGFGHRVYKNYDPRAKIMQKMCHAVLKETGHADDQMLKVAMELEKIALSDQYFID
CCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEC
RKLYPNVDFYSGITLKAMGFPVSMFTVLFAVARTVGWISQWSEMIEDPQQKIGRPRQLYT
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHC
GVTTRQYIDIDKRS
CCCCCEEEECCCCC
>Mature Secondary Structure
MDAKSESKTATLTVGNKNYDLPILSGTVGPDVIDIGKLYAQAGMFTYDPGFTSTGSCQSK
CCCCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCE
ITYIDGDAGVLEYRGYPIEQLAEKGDFLETCYLLLYGELPTPAQKKDFDYRVTHHTMVHE
EEEEECCCCEEEECCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHH
QMARFFQGFRRDAHPMAVMVASVGALAAFYHDSTDINDPKQRMIASIRMIAKIPTLAAMA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YKYSIGQPFVYPKNALSFSENFLNMCFAVPCEEYKPNPVLANALDKIFILHADHEQNAST
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHCEEEEEECCCCCCCCC
STVRIAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLAEIGTVDKIPEFIAKVKDKN
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCC
SDVRLMGFGHRVYKNYDPRAKIMQKMCHAVLKETGHADDQMLKVAMELEKIALSDQYFID
CCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEC
RKLYPNVDFYSGITLKAMGFPVSMFTVLFAVARTVGWISQWSEMIEDPQQKIGRPRQLYT
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHC
GVTTRQYIDIDKRS
CCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]