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The map label for this gene is gltX

Identifier: 146341054

GI number: 146341054

Start: 4318689

End: 4320125

Strand: Direct

Name: gltX

Synonym: BRADO4125

Alternate gene names: 146341054

Gene position: 4318689-4320125 (Clockwise)

Preceding gene: 146341052

Following gene: 146341055

Centisome position: 57.92

GC content: 66.25

Gene sequence:

>1437_bases
ATGACCTCATCGACCACCTCATCGATCGTCACGCGCTTTGCCCCCTCCCCGACCGGCTTTCTCCACATCGGAGGCGCCCG
GACGGCCCTGTTCAACTGGCTTTATGCGCGCGGCCGGGGCGGCAAGATGCTGCTCCGGATCGAGGATACCGACCGCGAGC
GCTCGACGACCGCGGCGATCGACGCCATCCTTGATGGGCTGAAATGGCTCGAGCTCGATTGGGACGGCGAGGTCATCTAT
CAATATAGCCGCGCGGCGCGGCATCGCGAGGTCGCCGAGCAGTTGCTCGCGACCGGCATGGCGTATCGCTGTTATGCCAC
CGCCGAGGAGCTGGTCGCCATGCGCGAGAAGGCGCGCGCCGAGGGCCGTACACGCCTGTACGACGGCATGTGGCGCGACC
GCGATCCCAAGGAGGCGCCCGAGGGCGTGAAGCCGACCATCCGCCTGCGCGCGCCGCTGACCGGCGAGACCATGGTCGAG
GACCAGGTGCAGGGCCGCGTGGTCTGGCAGAACGAGAACCTCGACGACCTTGTGCTGCTGCGAGGCGATGGCAATCCGAC
CTACATGCTGGCGGTGGTCGTCGACGACCACGACATGGGCGTCACCCACGTCATCCGCGGTGACGATCATTTGATCAACG
CCGCGCGCCAGAAGCAGATCTACGATGCGCTGGGCTGGACGGTGCCGTCGATGTCGCACATCCCGTTGATTCACGGGCCT
GACGGCTCGAAGCTGTCGAAGCGTCACGGCGCGCTCGGCGTCGATGCGTACCGCGCGATGGGATATTTGCCGTCCGCGCT
GCGCAATTATCTGGTGCGGCTCGGCTGGAGCCATGGCGATCAGGAGATCTTCTCGACCGAGGAGATGATCAAAGCCTTTG
ACCTGCCGGCGATCGGCCGCTCGGCGGCGCGTTTCGATTTCGCCAAGCTCGAGAACCTCAACGGCCATTACATCCGCCAC
AGCGACGATGCCGAACTGGTACGCCAGTTCGAGGATGTGCTGAACTACGTGCCAAACGGCGCGGCTCTGAAGGCCAAGCT
GAATGATGCCACCCGCGCGCAGCTTACGCAGGCGATGCCGAGCCTCAAGGAGCGCGCCAAGACGCTGCTCGAGCTGATCG
ACGGCGCCGCCTTCATCTTCGCCGATCGGCCGCTGCCGATCGACGCCAAGGCGGCCGCCCTGCTGACACCGGAGAGCCGC
GCTTTGATCGGCCGGCTGCACGCGGCACTTTCCGCCGTCGAGCCCTGGAGTGGGCCGGCCACCGAGGCCGCGCTGCGTGC
GTTTGCCGAGGCCAACAATCTCAAGCTGGGCGCAGTGGCCCAGCCGCTGCGCGCCGCGCTGACCGGGCGCACGACCTCGC
CCGGCATTTTCGACGTCCTGGCCATTTTGGGACGTGATGAATCCCTGGGCCGCCTGAAGGACCAGGCCATCGCATAA

Upstream 100 bases:

>100_bases
GCCAAATCCGCCTCCCCCAAGGAAGGCTTTAGTTATGCAATAGGCCTGCTATACACCCCCCGCCCTTTACAGCCCCTGCC
TGGATTTCGTGCTGCCGCCC

Downstream 100 bases:

>100_bases
GGTCTCATTAACCGCCATCTTGCAGCGCACATGGCAATACGGTACGCATTTGTGCGGCACGAATTGGTGTCCCGGGCTTT
GACAATCGTCACACGGTGCG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTSSTTSSIVTRFAPSPTGFLHIGGARTALFNWLYARGRGGKMLLRIEDTDRERSTTAAIDAILDGLKWLELDWDGEVIY
QYSRAARHREVAEQLLATGMAYRCYATAEELVAMREKARAEGRTRLYDGMWRDRDPKEAPEGVKPTIRLRAPLTGETMVE
DQVQGRVVWQNENLDDLVLLRGDGNPTYMLAVVVDDHDMGVTHVIRGDDHLINAARQKQIYDALGWTVPSMSHIPLIHGP
DGSKLSKRHGALGVDAYRAMGYLPSALRNYLVRLGWSHGDQEIFSTEEMIKAFDLPAIGRSAARFDFAKLENLNGHYIRH
SDDAELVRQFEDVLNYVPNGAALKAKLNDATRAQLTQAMPSLKERAKTLLELIDGAAFIFADRPLPIDAKAAALLTPESR
ALIGRLHAALSAVEPWSGPATEAALRAFAEANNLKLGAVAQPLRAALTGRTTSPGIFDVLAILGRDESLGRLKDQAIA

Sequences:

>Translated_478_residues
MTSSTTSSIVTRFAPSPTGFLHIGGARTALFNWLYARGRGGKMLLRIEDTDRERSTTAAIDAILDGLKWLELDWDGEVIY
QYSRAARHREVAEQLLATGMAYRCYATAEELVAMREKARAEGRTRLYDGMWRDRDPKEAPEGVKPTIRLRAPLTGETMVE
DQVQGRVVWQNENLDDLVLLRGDGNPTYMLAVVVDDHDMGVTHVIRGDDHLINAARQKQIYDALGWTVPSMSHIPLIHGP
DGSKLSKRHGALGVDAYRAMGYLPSALRNYLVRLGWSHGDQEIFSTEEMIKAFDLPAIGRSAARFDFAKLENLNGHYIRH
SDDAELVRQFEDVLNYVPNGAALKAKLNDATRAQLTQAMPSLKERAKTLLELIDGAAFIFADRPLPIDAKAAALLTPESR
ALIGRLHAALSAVEPWSGPATEAALRAFAEANNLKLGAVAQPLRAALTGRTTSPGIFDVLAILGRDESLGRLKDQAIA
>Mature_477_residues
TSSTTSSIVTRFAPSPTGFLHIGGARTALFNWLYARGRGGKMLLRIEDTDRERSTTAAIDAILDGLKWLELDWDGEVIYQ
YSRAARHREVAEQLLATGMAYRCYATAEELVAMREKARAEGRTRLYDGMWRDRDPKEAPEGVKPTIRLRAPLTGETMVED
QVQGRVVWQNENLDDLVLLRGDGNPTYMLAVVVDDHDMGVTHVIRGDDHLINAARQKQIYDALGWTVPSMSHIPLIHGPD
GSKLSKRHGALGVDAYRAMGYLPSALRNYLVRLGWSHGDQEIFSTEEMIKAFDLPAIGRSAARFDFAKLENLNGHYIRHS
DDAELVRQFEDVLNYVPNGAALKAKLNDATRAQLTQAMPSLKERAKTLLELIDGAAFIFADRPLPIDAKAAALLTPESRA
LIGRLHAALSAVEPWSGPATEAALRAFAEANNLKLGAVAQPLRAALTGRTTSPGIFDVLAILGRDESLGRLKDQAIA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=327, Percent_Identity=36.085626911315, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI4826960, Length=139, Percent_Identity=33.0935251798561, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788743, Length=468, Percent_Identity=43.8034188034188, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI226510925, Length=304, Percent_Identity=35.8552631578947, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1786895, Length=138, Percent_Identity=28.9855072463768, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=495, Percent_Identity=30.7070707070707, Blast_Score=194, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324540, Length=489, Percent_Identity=31.0838445807771, Blast_Score=227, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6321192, Length=212, Percent_Identity=28.3018867924528, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324742, Length=108, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24665321, Length=488, Percent_Identity=32.3770491803279, Blast_Score=238, Evalue=8e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_BRASO (A4YVF1)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001206102.1
- ProteinModelPortal:   A4YVF1
- SMR:   A4YVF1
- STRING:   A4YVF1
- GeneID:   5121004
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO4125
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   BSP376:BRADO4125-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52643; Mature: 52511

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 247-247

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSTTSSIVTRFAPSPTGFLHIGGARTALFNWLYARGRGGKMLLRIEDTDRERSTTAAI
CCCCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHH
DAILDGLKWLELDWDGEVIYQYSRAARHREVAEQLLATGMAYRCYATAEELVAMREKARA
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
EGRTRLYDGMWRDRDPKEAPEGVKPTIRLRAPLTGETMVEDQVQGRVVWQNENLDDLVLL
CCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCEEEEECCCCCEEEEE
RGDGNPTYMLAVVVDDHDMGVTHVIRGDDHLINAARQKQIYDALGWTVPSMSHIPLIHGP
EECCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
DGSKLSKRHGALGVDAYRAMGYLPSALRNYLVRLGWSHGDQEIFSTEEMIKAFDLPAIGR
CCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
SAARFDFAKLENLNGHYIRHSDDAELVRQFEDVLNYVPNGAALKAKLNDATRAQLTQAMP
HHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHH
SLKERAKTLLELIDGAAFIFADRPLPIDAKAAALLTPESRALIGRLHAALSAVEPWSGPA
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCH
TEAALRAFAEANNLKLGAVAQPLRAALTGRTTSPGIFDVLAILGRDESLGRLKDQAIA
HHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TSSTTSSIVTRFAPSPTGFLHIGGARTALFNWLYARGRGGKMLLRIEDTDRERSTTAAI
CCCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHH
DAILDGLKWLELDWDGEVIYQYSRAARHREVAEQLLATGMAYRCYATAEELVAMREKARA
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
EGRTRLYDGMWRDRDPKEAPEGVKPTIRLRAPLTGETMVEDQVQGRVVWQNENLDDLVLL
CCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCEEEEECCCCCEEEEE
RGDGNPTYMLAVVVDDHDMGVTHVIRGDDHLINAARQKQIYDALGWTVPSMSHIPLIHGP
EECCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
DGSKLSKRHGALGVDAYRAMGYLPSALRNYLVRLGWSHGDQEIFSTEEMIKAFDLPAIGR
CCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
SAARFDFAKLENLNGHYIRHSDDAELVRQFEDVLNYVPNGAALKAKLNDATRAQLTQAMP
HHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHH
SLKERAKTLLELIDGAAFIFADRPLPIDAKAAALLTPESRALIGRLHAALSAVEPWSGPA
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCH
TEAALRAFAEANNLKLGAVAQPLRAALTGRTTSPGIFDVLAILGRDESLGRLKDQAIA
HHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA