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The map label for this gene is kdsA

Identifier: 146341030

GI number: 146341030

Start: 4291193

End: 4292056

Strand: Reverse

Name: kdsA

Synonym: BRADO4101

Alternate gene names: 146341030

Gene position: 4292056-4291193 (Counterclockwise)

Preceding gene: 146341031

Following gene: 146341029

Centisome position: 57.56

GC content: 68.63

Gene sequence:

>864_bases
GTGAACGCTCCGATCTCCGCCGCACCGGTCGTGACTGCCGGCTCCGTCCGCTTCGGCAATCAACTGCCGCTGGCGGTGAT
CGCAGGTCCCTGCCAGCTCGAAAGCCGCGGCCACGCGCTCGAGGTCGCCTCGGCGCTGAAGGAGATCGCGACGCGGCTGA
ACATCAGCCTCGTCTACAAGACCTCGTTCGACAAGGCCAACCGCACCTCTGGCTCCGCCGCGCGTGGGCTCGGGCTTGCG
CAATCGCTGCCGATCTTCGCCGAGATCCGGTCGTCGCTCGGTCTTCCCGTGCTGACCGACGTGCATGATGCCAGCCAATG
CGCCGAGGTGGCGCAGGCGGTCGACATCCTGCAGATCCCGGCCTTCCTGTGCCGGCAGACGGATCTGCTGCTCGCAGCGG
CCGCGACCGGCAAGGTCGTCAACGTCAAGAAGGGCCAGTTCCTGGCGCCCTGGGACATGGCGAACGTCGTCGCCAAGATC
ACCGGCGCCGGCAATCCCAACGTGCTGGCGACCGAGCGCGGCGTCTCGTTCGGCTACAACACGCTCATCTCGGACATGCG
GTCGCTGCCGATCATGGCGAAGACGACCGGCGCGCCGGTCATCTTCGATGCGACGCATTCGGTGCAGCAGCCGGGCGGGC
AGGGCACCTCGTCGGGCGGCCAGCGCGAATTCGTCCCCGTGCTCGCACGCGCGGCCGTGGCGGTCGGCGTGGCCGGCGTG
TTCATCGAGACCCATCCCGATCCCGATCATGCGCCCTCCGACGGGCCGAACATGGTGCCGCTGGCGCAGTTCGAGGGACT
GTTGCGGACGCTGATGGCGTTCGATGCGCTCGCCAAGGGGCAGGCTGCGGACGCCATCCGCTGA

Upstream 100 bases:

>100_bases
GGATGACCGTCCCCGCAACCCTGATATTCGCGGGGTTCTGCCGGGTTGATCCCACGGGCCGGATTGGCCATGGTCGCGCG
CTCACAACGAGGATGATGCC

Downstream 100 bases:

>100_bases
TGCACCAGCCCGTCGCCAAGCCGTCTGATCAAAAGTCCGCTGACAACAAGTTTCCGCCGGCCGTCAACATCATCGCCTTG
GCTGGCTTCTCCGCGGCGCT

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNAPISAAPVVTAGSVRFGNQLPLAVIAGPCQLESRGHALEVASALKEIATRLNISLVYKTSFDKANRTSGSAARGLGLA
QSLPIFAEIRSSLGLPVLTDVHDASQCAEVAQAVDILQIPAFLCRQTDLLLAAAATGKVVNVKKGQFLAPWDMANVVAKI
TGAGNPNVLATERGVSFGYNTLISDMRSLPIMAKTTGAPVIFDATHSVQQPGGQGTSSGGQREFVPVLARAAVAVGVAGV
FIETHPDPDHAPSDGPNMVPLAQFEGLLRTLMAFDALAKGQAADAIR

Sequences:

>Translated_287_residues
MNAPISAAPVVTAGSVRFGNQLPLAVIAGPCQLESRGHALEVASALKEIATRLNISLVYKTSFDKANRTSGSAARGLGLA
QSLPIFAEIRSSLGLPVLTDVHDASQCAEVAQAVDILQIPAFLCRQTDLLLAAAATGKVVNVKKGQFLAPWDMANVVAKI
TGAGNPNVLATERGVSFGYNTLISDMRSLPIMAKTTGAPVIFDATHSVQQPGGQGTSSGGQREFVPVLARAAVAVGVAGV
FIETHPDPDHAPSDGPNMVPLAQFEGLLRTLMAFDALAKGQAADAIR
>Mature_287_residues
MNAPISAAPVVTAGSVRFGNQLPLAVIAGPCQLESRGHALEVASALKEIATRLNISLVYKTSFDKANRTSGSAARGLGLA
QSLPIFAEIRSSLGLPVLTDVHDASQCAEVAQAVDILQIPAFLCRQTDLLLAAAATGKVVNVKKGQFLAPWDMANVVAKI
TGAGNPNVLATERGVSFGYNTLISDMRSLPIMAKTTGAPVIFDATHSVQQPGGQGTSSGGQREFVPVLARAAVAVGVAGV
FIETHPDPDHAPSDGPNMVPLAQFEGLLRTLMAFDALAKGQAADAIR

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=272, Percent_Identity=47.0588235294118, Blast_Score=258, Evalue=3e-70,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_BRASO (A4YVC7)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001206078.1
- ProteinModelPortal:   A4YVC7
- SMR:   A4YVC7
- STRING:   A4YVC7
- GeneID:   5121237
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO4101
- eggNOG:   COG2877
- HOGENOM:   HBG575990
- OMA:   RSLPIMA
- ProtClustDB:   PRK05198
- BioCyc:   BSP376:BRADO4101-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 29684; Mature: 29684

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPISAAPVVTAGSVRFGNQLPLAVIAGPCQLESRGHALEVASALKEIATRLNISLVYK
CCCCCCCCCEEEECCEECCCCCCEEEEECCEEECCCCCHHHHHHHHHHHHHHCCEEEEEE
TSFDKANRTSGSAARGLGLAQSLPIFAEIRSSLGLPVLTDVHDASQCAEVAQAVDILQIP
ECCCCCCCCCCCHHHCCCCHHCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHH
AFLCRQTDLLLAAAATGKVVNVKKGQFLAPWDMANVVAKITGAGNPNVLATERGVSFGYN
HHHHCCHHHEEEEECCCCEEEECCCCEECCHHHHHHHHHHCCCCCCCEEEECCCCCCCHH
TLISDMRSLPIMAKTTGAPVIFDATHSVQQPGGQGTSSGGQREFVPVLARAAVAVGVAGV
HHHHHHHHCCEEEECCCCCEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEE
FIETHPDPDHAPSDGPNMVPLAQFEGLLRTLMAFDALAKGQAADAIR
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNAPISAAPVVTAGSVRFGNQLPLAVIAGPCQLESRGHALEVASALKEIATRLNISLVYK
CCCCCCCCCEEEECCEECCCCCCEEEEECCEEECCCCCHHHHHHHHHHHHHHCCEEEEEE
TSFDKANRTSGSAARGLGLAQSLPIFAEIRSSLGLPVLTDVHDASQCAEVAQAVDILQIP
ECCCCCCCCCCCHHHCCCCHHCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHH
AFLCRQTDLLLAAAATGKVVNVKKGQFLAPWDMANVVAKITGAGNPNVLATERGVSFGYN
HHHHCCHHHEEEEECCCCEEEECCCCEECCHHHHHHHHHHCCCCCCCEEEECCCCCCCHH
TLISDMRSLPIMAKTTGAPVIFDATHSVQQPGGQGTSSGGQREFVPVLARAAVAVGVAGV
HHHHHHHHCCEEEECCCCCEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEE
FIETHPDPDHAPSDGPNMVPLAQFEGLLRTLMAFDALAKGQAADAIR
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA