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The map label for this gene is insF1 [C]

Identifier: 146341026

GI number: 146341026

Start: 4286627

End: 4287484

Strand: Reverse

Name: insF1 [C]

Synonym: BRADO4097

Alternate gene names: 146341026

Gene position: 4287484-4286627 (Counterclockwise)

Preceding gene: 146341027

Following gene: 146341023

Centisome position: 57.5

GC content: 62.0

Gene sequence:

>858_bases
ATGACCGCGCAGACAGCCGACTCCAGCGCTCATGTGCAGCGCCTCTGCGCTTTGGCAGGGTTGCCTCGAGCGACTTATTA
CCGGCATCTCAATCGACGCAGCCGCGCCGAGGCCGAGTGCGAGTTGCGCGACCAGCTCCAGCGCATCTGCCTGAAGCATC
CGTTCTACGGCTATCGACGGGTGACCGCCGCTCTTCGGCGTCTGGGGATGGCCGTCAATGCCAAGAAAGTCCTGCGGCTC
ATGCGGCAGGATAATCTTCTCGCGCAGCGCAAGACGCCGTTCCTGAAGCCGCCGACTGAGAGGCCGACTGACGTGATCGT
CGTCCCCAACCTGATCCGTGGCCTGGCGCCATCGGCTCCGGATCAGATCTGGGTCGCTGACATCACCTACGTCCATCTCG
CCAAGACGTTCGCCTATCTCGCCGTCATTCTCGACGGCTTCTCCCGCAAGGCGGTCGGCTGGGCATTTGACAACACCCTC
GACGCCTCGCTCGCCATCGCGGCGCTCGACAAAGCGCTCAAATCCCGGAATCCCAAGCCGGGCAGCCTGATCCACCACTC
CGACCGCGGCGTGCAATACGCCTCGATCGCTTATCGCCAGCGCCTCGCCGATCGCGAGATCAAGATCAGCATGAGCAGTC
CAGGCAACCCCTTCGACAACGCCAAGGCCGAAAGCTTCATGAAGACGCTCAAGGCCGAGGAGGTCAACGGCAAGACCTTT
GCCGACGTCAACGACGCCCGGCGCCGGATCAACAGCTTCATCGCCGAGATCTACAACAAGGAGCGCTTGCATTCGGCCCT
CGGCTATCGATCTCCCCTTGAGTTCGAAGCCGCGTTCAAGCAAAACACAATGCGATGA

Upstream 100 bases:

>100_bases
AGCAGATGGACCTCGATTTTTTTCGGCAAGCCTTGCGCGCACTGGAGCGGCCGGCAGCGCAAGGCAAACCCGTATCCGCA
TCATCGAAGTCATCAAAAGC

Downstream 100 bases:

>100_bases
CTTCACCCACTGTCACTGAGATTATCGTGTCTCACTTGAGGGGTGCAGTCCAGCACTGTCACCGTATTCTCAGTGATCTC
GCGTTTAAAGGCGATAGCGC

Product: transposase

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTAQTADSSAHVQRLCALAGLPRATYYRHLNRRSRAEAECELRDQLQRICLKHPFYGYRRVTAALRRLGMAVNAKKVLRL
MRQDNLLAQRKTPFLKPPTERPTDVIVVPNLIRGLAPSAPDQIWVADITYVHLAKTFAYLAVILDGFSRKAVGWAFDNTL
DASLAIAALDKALKSRNPKPGSLIHHSDRGVQYASIAYRQRLADREIKISMSSPGNPFDNAKAESFMKTLKAEEVNGKTF
ADVNDARRRINSFIAEIYNKERLHSALGYRSPLEFEAAFKQNTMR

Sequences:

>Translated_285_residues
MTAQTADSSAHVQRLCALAGLPRATYYRHLNRRSRAEAECELRDQLQRICLKHPFYGYRRVTAALRRLGMAVNAKKVLRL
MRQDNLLAQRKTPFLKPPTERPTDVIVVPNLIRGLAPSAPDQIWVADITYVHLAKTFAYLAVILDGFSRKAVGWAFDNTL
DASLAIAALDKALKSRNPKPGSLIHHSDRGVQYASIAYRQRLADREIKISMSSPGNPFDNAKAESFMKTLKAEEVNGKTF
ADVNDARRRINSFIAEIYNKERLHSALGYRSPLEFEAAFKQNTMR
>Mature_284_residues
TAQTADSSAHVQRLCALAGLPRATYYRHLNRRSRAEAECELRDQLQRICLKHPFYGYRRVTAALRRLGMAVNAKKVLRLM
RQDNLLAQRKTPFLKPPTERPTDVIVVPNLIRGLAPSAPDQIWVADITYVHLAKTFAYLAVILDGFSRKAVGWAFDNTLD
ASLAIAALDKALKSRNPKPGSLIHHSDRGVQYASIAYRQRLADREIKISMSSPGNPFDNAKAESFMKTLKAEEVNGKTFA
DVNDARRRINSFIAEIYNKERLHSALGYRSPLEFEAAFKQNTMR

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=237, Percent_Identity=31.6455696202532, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1788405, Length=271, Percent_Identity=33.210332103321, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787263, Length=271, Percent_Identity=33.210332103321, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786753, Length=271, Percent_Identity=33.210332103321, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786570, Length=271, Percent_Identity=33.210332103321, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786490, Length=271, Percent_Identity=33.210332103321, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1789981, Length=259, Percent_Identity=30.8880308880309, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790724, Length=259, Percent_Identity=31.2741312741313, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789424, Length=259, Percent_Identity=31.2741312741313, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789224, Length=259, Percent_Identity=31.2741312741313, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788305, Length=259, Percent_Identity=31.2741312741313, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787669, Length=259, Percent_Identity=31.2741312741313, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786558, Length=259, Percent_Identity=31.2741312741313, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32183; Mature: 32052

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQTADSSAHVQRLCALAGLPRATYYRHLNRRSRAEAECELRDQLQRICLKHPFYGYRR
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHH
VTAALRRLGMAVNAKKVLRLMRQDNLLAQRKTPFLKPPTERPTDVIVVPNLIRGLAPSAP
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHCCCCCCC
DQIWVADITYVHLAKTFAYLAVILDGFSRKAVGWAFDNTLDASLAIAALDKALKSRNPKP
CCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
GSLIHHSDRGVQYASIAYRQRLADREIKISMSSPGNPFDNAKAESFMKTLKAEEVNGKTF
CCEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC
ADVNDARRRINSFIAEIYNKERLHSALGYRSPLEFEAAFKQNTMR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TAQTADSSAHVQRLCALAGLPRATYYRHLNRRSRAEAECELRDQLQRICLKHPFYGYRR
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHH
VTAALRRLGMAVNAKKVLRLMRQDNLLAQRKTPFLKPPTERPTDVIVVPNLIRGLAPSAP
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHCCCCCCC
DQIWVADITYVHLAKTFAYLAVILDGFSRKAVGWAFDNTLDASLAIAALDKALKSRNPKP
CCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
GSLIHHSDRGVQYASIAYRQRLADREIKISMSSPGNPFDNAKAESFMKTLKAEEVNGKTF
CCEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC
ADVNDARRRINSFIAEIYNKERLHSALGYRSPLEFEAAFKQNTMR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]