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The map label for this gene is eno [H]

Identifier: 146341023

GI number: 146341023

Start: 4284247

End: 4285530

Strand: Reverse

Name: eno [H]

Synonym: BRADO4094

Alternate gene names: 146341023

Gene position: 4285530-4284247 (Counterclockwise)

Preceding gene: 146341026

Following gene: 146341022

Centisome position: 57.47

GC content: 65.5

Gene sequence:

>1284_bases
ATGACCGCCATCGTCGACATCATCGGCCGCGAAATCCTGGACAGCCGCGGCAATCCCACGGTCGAGGTCGACGTCGTGCT
GGAGGACGGCTCGGTCGGTCGCGCCGCCGTGCCCTCGGGCGCCTCCACCGGCGCCCATGAGGCGGTCGAGCTGCGCGACG
GCGACAAGCGCCGCTATCTCGGCAAGGGTGTGCAGAAGGCGGTCGAGGCAATCAACGACGAGATCTATGAGGCGCTCAGC
GACATGTCGGTGCAGGACCAGGTCCAGATCGACCAGATCCTGATCGAGCTCGACGGCACCGAGAACAAGAGCCGGCTCGG
CGCCAATGCCATCCTCGGCGTGTCGCTGGCCTGCGCCAAGGCGGCGGCGATCTCCTATGACATGCCGCTGTATCGCTATG
TCGGCGGCACATCGGCGCGCACCCTGCCGGTGCCGATGATGAACATCGTCAATGGCGGCGTCCACGCCGACAACCCGATC
GACTTCCAGGAATTCATGATCATGCCAGTGGGCGCGCCGAGCTTCGCGGAAGCGCTGCGCTGCGGCTCGGAGATCTTCCA
TACGCTCAAGGGCGAGCTGAAGAAGGCCGGCCACAACACCAATGTCGGCGATGAAGGCGGTTTCGCGCCGAACCTGCCGT
CGGCGGACGCGGCCCTCGACTTCGTCATGGCCGCGATCGGCAAGGCCGGCTACACCGCGGGCGAAGACGTGATGCTGGCG
CTCGACTGCGCCGCGACCGAGTTCTTCAAGGACGGCAAGTATGTCTACGAGGGCGAGAACAAGAGCCGCTCGCGCTCCGA
GCAGGCGAAGTATCTCGCCGACCTCGTCGCGCGCTATCCGATCTGCTCGATCGAGGACGGCATGTCCGAGGACGACATGG
ACGGCTGGAAGGAACTCACCGACCTGATCGGCCACAAGTGCCAGCTGGTCGGCGACGATCTGTTCGTGACCAATGTGACC
CGTCTCGAGGACGGCATCCGCAACGGTCGCGCCAACTCGATCCTGATCAAGGTCAACCAGATCGGCACGCTGACCGAGAC
CTTGGCCGCGGTCGAGATGGCCTACAAGGCCGGCTACACGGCCGTGATGTCGCATCGCTCGGGCGAGACCGAGGATTCGA
CCATCGCCGACCTCGCGGTCGCGACCAATTGCGGACAGATCAAGACCGGCTCGCTGGCGCGCTCCGACCGCACCGCCAAG
TACAACCAGCTGTTGCGCATCGAGCAGGAGCTGGACGCGCAGGCCAAATATGCCGGTCGGGCGGCGCTGAAGGCGCTGGC
CTGA

Upstream 100 bases:

>100_bases
TTCCAGGCGACTTCCGCGCGTCTTGGCCTTTTCGGACCCCCGGGGTTGCGGTAGAAGCGGCGCAGCAACGCCCACCCGAC
AAGCTCGCAAGAGGACCCCC

Downstream 100 bases:

>100_bases
GCCGACGGCAAGACAAGCAACAGGGGAGGCACGCATGAGCGTCGAGACACAAGGCCTGCAGCTGCGGACGCTGATCAAGC
GCAGCGGCGAGCTTGAGCTG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTAIVDIIGREILDSRGNPTVEVDVVLEDGSVGRAAVPSGASTGAHEAVELRDGDKRRYLGKGVQKAVEAINDEIYEALS
DMSVQDQVQIDQILIELDGTENKSRLGANAILGVSLACAKAAAISYDMPLYRYVGGTSARTLPVPMMNIVNGGVHADNPI
DFQEFMIMPVGAPSFAEALRCGSEIFHTLKGELKKAGHNTNVGDEGGFAPNLPSADAALDFVMAAIGKAGYTAGEDVMLA
LDCAATEFFKDGKYVYEGENKSRSRSEQAKYLADLVARYPICSIEDGMSEDDMDGWKELTDLIGHKCQLVGDDLFVTNVT
RLEDGIRNGRANSILIKVNQIGTLTETLAAVEMAYKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAK
YNQLLRIEQELDAQAKYAGRAALKALA

Sequences:

>Translated_427_residues
MTAIVDIIGREILDSRGNPTVEVDVVLEDGSVGRAAVPSGASTGAHEAVELRDGDKRRYLGKGVQKAVEAINDEIYEALS
DMSVQDQVQIDQILIELDGTENKSRLGANAILGVSLACAKAAAISYDMPLYRYVGGTSARTLPVPMMNIVNGGVHADNPI
DFQEFMIMPVGAPSFAEALRCGSEIFHTLKGELKKAGHNTNVGDEGGFAPNLPSADAALDFVMAAIGKAGYTAGEDVMLA
LDCAATEFFKDGKYVYEGENKSRSRSEQAKYLADLVARYPICSIEDGMSEDDMDGWKELTDLIGHKCQLVGDDLFVTNVT
RLEDGIRNGRANSILIKVNQIGTLTETLAAVEMAYKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAK
YNQLLRIEQELDAQAKYAGRAALKALA
>Mature_426_residues
TAIVDIIGREILDSRGNPTVEVDVVLEDGSVGRAAVPSGASTGAHEAVELRDGDKRRYLGKGVQKAVEAINDEIYEALSD
MSVQDQVQIDQILIELDGTENKSRLGANAILGVSLACAKAAAISYDMPLYRYVGGTSARTLPVPMMNIVNGGVHADNPID
FQEFMIMPVGAPSFAEALRCGSEIFHTLKGELKKAGHNTNVGDEGGFAPNLPSADAALDFVMAAIGKAGYTAGEDVMLAL
DCAATEFFKDGKYVYEGENKSRSRSEQAKYLADLVARYPICSIEDGMSEDDMDGWKELTDLIGHKCQLVGDDLFVTNVTR
LEDGIRNGRANSILIKVNQIGTLTETLAAVEMAYKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKY
NQLLRIEQELDAQAKYAGRAALKALA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=434, Percent_Identity=52.7649769585253, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI4503571, Length=437, Percent_Identity=52.6315789473684, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=51.8604651162791, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=51.8604651162791, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=46.2616822429907, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=25.8160237388724, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=25.8160237388724, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=25.8160237388724, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1789141, Length=432, Percent_Identity=62.2685185185185, Blast_Score=513, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71995829, Length=433, Percent_Identity=52.6558891454965, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=52.6558891454965, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=193, Percent_Identity=46.1139896373057, Blast_Score=170, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=50.6944444444444, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.4226327944573, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.1916859122402, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.1916859122402, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=49.7685185185185, Blast_Score=379, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=51.2761020881671, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=51.2761020881671, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=51.2761020881671, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=51.2761020881671, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=51.2761020881671, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=51.2761020881671, Blast_Score=391, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45702; Mature: 45571

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIVDIIGREILDSRGNPTVEVDVVLEDGSVGRAAVPSGASTGAHEAVELRDGDKRRYL
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
GKGVQKAVEAINDEIYEALSDMSVQDQVQIDQILIELDGTENKSRLGANAILGVSLACAK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEEEECCCCCHHHHCCHHHHHHHHHHHH
AAAISYDMPLYRYVGGTSARTLPVPMMNIVNGGVHADNPIDFQEFMIMPVGAPSFAEALR
HHHHCCCCCHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
CGSEIFHTLKGELKKAGHNTNVGDEGGFAPNLPSADAALDFVMAAIGKAGYTAGEDVMLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAATEFFKDGKYVYEGENKSRSRSEQAKYLADLVARYPICSIEDGMSEDDMDGWKELT
EEHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
DLIGHKCQLVGDDLFVTNVTRLEDGIRNGRANSILIKVNQIGTLTETLAAVEMAYKAGYT
HHHCCEEEEECCCEEEEHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCH
AVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKYNQLLRIEQELDAQAKYAGR
HHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AALKALA
HHHHHCC
>Mature Secondary Structure 
TAIVDIIGREILDSRGNPTVEVDVVLEDGSVGRAAVPSGASTGAHEAVELRDGDKRRYL
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
GKGVQKAVEAINDEIYEALSDMSVQDQVQIDQILIELDGTENKSRLGANAILGVSLACAK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEEEECCCCCHHHHCCHHHHHHHHHHHH
AAAISYDMPLYRYVGGTSARTLPVPMMNIVNGGVHADNPIDFQEFMIMPVGAPSFAEALR
HHHHCCCCCHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
CGSEIFHTLKGELKKAGHNTNVGDEGGFAPNLPSADAALDFVMAAIGKAGYTAGEDVMLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAATEFFKDGKYVYEGENKSRSRSEQAKYLADLVARYPICSIEDGMSEDDMDGWKELT
EEHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
DLIGHKCQLVGDDLFVTNVTRLEDGIRNGRANSILIKVNQIGTLTETLAAVEMAYKAGYT
HHHCCEEEEECCCEEEEHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCH
AVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKYNQLLRIEQELDAQAKYAGR
HHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AALKALA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA