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The map label for this gene is pdhB [H]

Identifier: 146341015

GI number: 146341015

Start: 4275783

End: 4277180

Strand: Reverse

Name: pdhB [H]

Synonym: BRADO4086

Alternate gene names: 146341015

Gene position: 4277180-4275783 (Counterclockwise)

Preceding gene: 146341016

Following gene: 146341014

Centisome position: 57.36

GC content: 66.88

Gene sequence:

>1398_bases
ATGCCTATTCAAGTTTTGATGCCTGCGCTGTCGCCCACGATGGAGAAGGGCAACCTCGCCAAATGGCTGAAGAAGGAAGG
CGAGGCGATCAAGTCGGGTGACGTGATCGCCGAGATCGAGACCGACAAGGCGACGATGGAGGTCGAGGCGACCGACGAGG
GCACGCTCGGCAAGATCCTGATCCCCGAAGGCACAGCCGACGTTGCGGTCAACACGCCGATCGCCACGATTCTCGCGGAT
GGCGAAACGGCGGCCGACCTCGGCAAGGCCTCGGCGCCCGCCGCCGAGATGAAGGCCGCGCAGTCGGCGCCGCCGGCTGA
CGCTGGTGTCTCCGTGCAGGCCTCGCCCGCGCCGACCGGCGTAGCGGCGCCGCAGAGCGTCGCCGAGCCCGATCCGGAAG
TCCCCGCCGGCACCGAGATGGTGACGCAGACCATCCGCGAGGCGCTGCGCGACGCGATGGCCGAGGAGATGCGCCGCGAC
GGCGACGTCTTCATCTTGGGCGAGGAAGTCGCCGAATATCAGGGCGCCTACAAGGTGACCCAGGGCCTGTTGCAGGAGTT
CGGCGCCCGCCGCGTGATGGACACGCCGATCACCGAGCACGGCTTCGCCGGCATCGGTGTCGGCGCGGCGATGGCCGGCC
TGAAGCCGATCGTCGAGTTCATGACCTTCAACTTCGCCATGCAGGCGATCGACCAGATCATCAACTCCGCGGCCAAAACC
TTGTACATGTCCGGCGGCCAGATGGGCTGCTCGATCGTGTTCCGCGGGCCTAACGGCGCCGCCGCCCGCGTCGCCGCGCA
GCACAGCCAGGACTACTCGTCGTGGTATTCGCACATCCCCGGCCTCAAGGTCGTCGCGCCGTATTCCGCGGCCGATGCCA
AAGGCTTGTTGAAGGCCGCGATCCGCGATCCCAACCCGGTGATCTTCCTCGAGAACGAGGTGCTGTATGGTCACTCCGGC
GAGGTGCCGAAGCTCGACGACTACATCATCCCGATCGGCAAGGCGCGTATCGCCCGCACCGGCAAGGACGTCACGATCAT
CTCCTGGTCGAACGGCATGACCTACGCCTTGAAGGCCGCCGATGAGCTCGCCAAGGAGGGCATCGAGGCCGAGGTGATCG
ACCTGCGCACCCTGCGTCCGATGGACACCGACACCATCATCGCCTCGGTCAAGAAGACCGGGCGCGCGGTGACGGTGGAA
GAGGGCTGGGCGCAGAGCGGCGTCGGCGCCGAGATCGCCGCGCGCATCATGGAGCACGCCTTCGACTATCTCGATGCGCC
GGTCACGCGCGTGTCGGGCAAGGACGTGCCGATGCCGTATGCCGCGAACCTCGAGAAGCTCGCGCTGCCGTCGGCTGCGG
AGGTCGTGCAGGCCGCCAAGTCCGTCTGCTACCGCTAA

Upstream 100 bases:

>100_bases
CGCCGATTTCGCGCAGGCCGATCCCGAGCCGGATCCCGCCGAGCTCTACACCGACGTCTATCGCTAATCGCGCGCGCAAG
CTGATTTCCCTGGAGCTGAT

Downstream 100 bases:

>100_bases
GGAAATCCCAGATGGCCGGACCCAAGGAGCAGCCGCTGCCGCCCGACGTCGTCGGCCGCGAGGACGCCACCGAAGTGTTG
CGCGCCTTCGTGCTCGACGG

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MPIQVLMPALSPTMEKGNLAKWLKKEGEAIKSGDVIAEIETDKATMEVEATDEGTLGKILIPEGTADVAVNTPIATILAD
GETAADLGKASAPAAEMKAAQSAPPADAGVSVQASPAPTGVAAPQSVAEPDPEVPAGTEMVTQTIREALRDAMAEEMRRD
GDVFILGEEVAEYQGAYKVTQGLLQEFGARRVMDTPITEHGFAGIGVGAAMAGLKPIVEFMTFNFAMQAIDQIINSAAKT
LYMSGGQMGCSIVFRGPNGAAARVAAQHSQDYSSWYSHIPGLKVVAPYSAADAKGLLKAAIRDPNPVIFLENEVLYGHSG
EVPKLDDYIIPIGKARIARTGKDVTIISWSNGMTYALKAADELAKEGIEAEVIDLRTLRPMDTDTIIASVKKTGRAVTVE
EGWAQSGVGAEIAARIMEHAFDYLDAPVTRVSGKDVPMPYAANLEKLALPSAAEVVQAAKSVCYR

Sequences:

>Translated_465_residues
MPIQVLMPALSPTMEKGNLAKWLKKEGEAIKSGDVIAEIETDKATMEVEATDEGTLGKILIPEGTADVAVNTPIATILAD
GETAADLGKASAPAAEMKAAQSAPPADAGVSVQASPAPTGVAAPQSVAEPDPEVPAGTEMVTQTIREALRDAMAEEMRRD
GDVFILGEEVAEYQGAYKVTQGLLQEFGARRVMDTPITEHGFAGIGVGAAMAGLKPIVEFMTFNFAMQAIDQIINSAAKT
LYMSGGQMGCSIVFRGPNGAAARVAAQHSQDYSSWYSHIPGLKVVAPYSAADAKGLLKAAIRDPNPVIFLENEVLYGHSG
EVPKLDDYIIPIGKARIARTGKDVTIISWSNGMTYALKAADELAKEGIEAEVIDLRTLRPMDTDTIIASVKKTGRAVTVE
EGWAQSGVGAEIAARIMEHAFDYLDAPVTRVSGKDVPMPYAANLEKLALPSAAEVVQAAKSVCYR
>Mature_464_residues
PIQVLMPALSPTMEKGNLAKWLKKEGEAIKSGDVIAEIETDKATMEVEATDEGTLGKILIPEGTADVAVNTPIATILADG
ETAADLGKASAPAAEMKAAQSAPPADAGVSVQASPAPTGVAAPQSVAEPDPEVPAGTEMVTQTIREALRDAMAEEMRRDG
DVFILGEEVAEYQGAYKVTQGLLQEFGARRVMDTPITEHGFAGIGVGAAMAGLKPIVEFMTFNFAMQAIDQIINSAAKTL
YMSGGQMGCSIVFRGPNGAAARVAAQHSQDYSSWYSHIPGLKVVAPYSAADAKGLLKAAIRDPNPVIFLENEVLYGHSGE
VPKLDDYIIPIGKARIARTGKDVTIISWSNGMTYALKAADELAKEGIEAEVIDLRTLRPMDTDTIIASVKKTGRAVTVEE
GWAQSGVGAEIAARIMEHAFDYLDAPVTRVSGKDVPMPYAANLEKLALPSAAEVVQAAKSVCYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=321, Percent_Identity=60.7476635514019, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI291084858, Length=321, Percent_Identity=57.3208722741433, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI4557353, Length=337, Percent_Identity=35.9050445103858, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI34101272, Length=337, Percent_Identity=35.9050445103858, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI203098753, Length=81, Percent_Identity=53.0864197530864, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI203098816, Length=81, Percent_Identity=53.0864197530864, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI31711992, Length=85, Percent_Identity=54.1176470588235, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI260898739, Length=81, Percent_Identity=51.8518518518518, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI133778974, Length=154, Percent_Identity=33.7662337662338, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI225637459, Length=242, Percent_Identity=29.7520661157025, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI225637461, Length=242, Percent_Identity=29.7520661157025, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI225637463, Length=242, Percent_Identity=30.1652892561983, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=323, Percent_Identity=63.4674922600619, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17506935, Length=343, Percent_Identity=40.2332361516035, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17560088, Length=133, Percent_Identity=45.1127819548872, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17539652, Length=258, Percent_Identity=30.6201550387597, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=60.5504587155963, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324258, Length=131, Percent_Identity=48.8549618320611, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321632, Length=113, Percent_Identity=45.1327433628319, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21358145, Length=325, Percent_Identity=61.8461538461539, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24650940, Length=325, Percent_Identity=61.8461538461539, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI160714832, Length=319, Percent_Identity=38.2445141065831, Blast_Score=217, Evalue=2e-56,
Organism=Drosophila melanogaster, GI160714828, Length=319, Percent_Identity=38.2445141065831, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24650943, Length=89, Percent_Identity=66.2921348314607, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24650945, Length=89, Percent_Identity=66.2921348314607, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI20129315, Length=84, Percent_Identity=53.5714285714286, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45551847, Length=241, Percent_Identity=28.2157676348548, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550715, Length=241, Percent_Identity=28.2157676348548, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24645119, Length=241, Percent_Identity=28.2157676348548, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24582497, Length=73, Percent_Identity=50.6849315068493, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 49043; Mature: 48912

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIQVLMPALSPTMEKGNLAKWLKKEGEAIKSGDVIAEIETDKATMEVEATDEGTLGKIL
CCCEEEEHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCEEE
IPEGTADVAVNTPIATILADGETAADLGKASAPAAEMKAAQSAPPADAGVSVQASPAPTG
ECCCCCCEEECCCEEEEEECCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEECCCCCCC
VAAPQSVAEPDPEVPAGTEMVTQTIREALRDAMAEEMRRDGDVFILGEEVAEYQGAYKVT
CCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHH
QGLLQEFGARRVMDTPITEHGFAGIGVGAAMAGLKPIVEFMTFNFAMQAIDQIINSAAKT
HHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYMSGGQMGCSIVFRGPNGAAARVAAQHSQDYSSWYSHIPGLKVVAPYSAADAKGLLKAA
EEECCCCCCEEEEEECCCCCHHHHHHHHCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
IRDPNPVIFLENEVLYGHSGEVPKLDDYIIPIGKARIARTGKDVTIISWSNGMTYALKAA
HCCCCCEEEEECEEEECCCCCCCCCCCEEEECCCHHHHCCCCCEEEEEECCCCEEEHHHH
DELAKEGIEAEVIDLRTLRPMDTDTIIASVKKTGRAVTVEEGWAQSGVGAEIAARIMEHA
HHHHHCCCCEEEEEHECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHH
FDYLDAPVTRVSGKDVPMPYAANLEKLALPSAAEVVQAAKSVCYR
HHHHCCCHHHCCCCCCCCCHHCCHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PIQVLMPALSPTMEKGNLAKWLKKEGEAIKSGDVIAEIETDKATMEVEATDEGTLGKIL
CCEEEEHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCEEE
IPEGTADVAVNTPIATILADGETAADLGKASAPAAEMKAAQSAPPADAGVSVQASPAPTG
ECCCCCCEEECCCEEEEEECCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEECCCCCCC
VAAPQSVAEPDPEVPAGTEMVTQTIREALRDAMAEEMRRDGDVFILGEEVAEYQGAYKVT
CCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHH
QGLLQEFGARRVMDTPITEHGFAGIGVGAAMAGLKPIVEFMTFNFAMQAIDQIINSAAKT
HHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYMSGGQMGCSIVFRGPNGAAARVAAQHSQDYSSWYSHIPGLKVVAPYSAADAKGLLKAA
EEECCCCCCEEEEEECCCCCHHHHHHHHCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
IRDPNPVIFLENEVLYGHSGEVPKLDDYIIPIGKARIARTGKDVTIISWSNGMTYALKAA
HCCCCCEEEEECEEEECCCCCCCCCCCEEEECCCHHHHCCCCCEEEEEECCCCEEEHHHH
DELAKEGIEAEVIDLRTLRPMDTDTIIASVKKTGRAVTVEEGWAQSGVGAEIAARIMEHA
HHHHHCCCCEEEEEHECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHH
FDYLDAPVTRVSGKDVPMPYAANLEKLALPSAAEVVQAAKSVCYR
HHHHCCCHHHCCCCCCCCCHHCCHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]