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The map label for this gene is xthA2 [H]

Identifier: 146340991

GI number: 146340991

Start: 4251966

End: 4252757

Strand: Direct

Name: xthA2 [H]

Synonym: BRADO4062

Alternate gene names: 146340991

Gene position: 4251966-4252757 (Clockwise)

Preceding gene: 146340989

Following gene: 146340992

Centisome position: 57.02

GC content: 63.76

Gene sequence:

>792_bases
ATGCGCATCGCCACCTGGAACGTCAATTCGATCCGGCAACGGATCGAGCACCTCGTGACCTGGCTCAAGGAATGCTCGCC
GGACATCGTCTGCCTGCAGGAGATCAAGTGCACCGACGACGCGTTTCCCAGACTGGAGATCGAAGCGCTCGGCTACAACG
TCGTCACCCACGGGCAGAAGACGTTCAACGGCGTCGCGCTGCTGTCGAAGCTGCCGTTCGACGAGACCAAGTCCGGGCTC
GACGGCGACGCCGAGGACGTGCATGCGCGGTTCCTCGAAGGCGTGGTCAGCCTCAAGAGCGGCGTGGTGCGCATCGCCTG
CCTCTATCTGCCCAACGGCAACCCGCCAAACACCGAGAAATATCCCTACAAGCTCAAATGGATGTCGCGGCTTCGTGACT
ATGCGCGTGAACGGCTGAAGACCGAGGAGCCGCTGATCCTCGCGGGCGACTTCAACGTCATCCCCGCGGCGGCCGATGTC
TCCAATCCCGAGGCCTGGGTCGAGGACGCGCTGTTCCGGCCCGAGACACGCGAAAGCTTTCAGTCGCTGCTCGGCCTCGG
GCTGACCGACGCGCTGCGCGCCGTCAATGACGCGCCGGGGCAGTACACGTTCTGGGACTACCAGGCCGGCGCCTGGCAGA
AGAACGCAGGGATTCGCATCGATCACCTGTTGTTGTCGCCGCAGGCCAGCGACAGGCTGGTCGAGGTGGGCATCGACAGC
TATGTCAGGGCTTGGGAGAAGCCGTCCGATCACGTCCCGGTGTGGGCCGATTTCGATCTGGAGACGGCGTAA

Upstream 100 bases:

>100_bases
TGTTGCGCGCGGCTGTGAGTCCCCCCGCCAACCGCCTTGACCCGGAGCGCCGCATCTTGATCTAACCGGACGAACCCTCC
GAGCTTCCTGGAACCATCCG

Downstream 100 bases:

>100_bases
CGTTCAAGGCAGGTCCGATGAGCTTCGTGACACGGCGGCAGGTGTTTCCGCTCGCGCTCGCACCTCTGTTCGTGAAGCCG
GCGTTGGCTGCCGACTTCCA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MRIATWNVNSIRQRIEHLVTWLKECSPDIVCLQEIKCTDDAFPRLEIEALGYNVVTHGQKTFNGVALLSKLPFDETKSGL
DGDAEDVHARFLEGVVSLKSGVVRIACLYLPNGNPPNTEKYPYKLKWMSRLRDYARERLKTEEPLILAGDFNVIPAAADV
SNPEAWVEDALFRPETRESFQSLLGLGLTDALRAVNDAPGQYTFWDYQAGAWQKNAGIRIDHLLLSPQASDRLVEVGIDS
YVRAWEKPSDHVPVWADFDLETA

Sequences:

>Translated_263_residues
MRIATWNVNSIRQRIEHLVTWLKECSPDIVCLQEIKCTDDAFPRLEIEALGYNVVTHGQKTFNGVALLSKLPFDETKSGL
DGDAEDVHARFLEGVVSLKSGVVRIACLYLPNGNPPNTEKYPYKLKWMSRLRDYARERLKTEEPLILAGDFNVIPAAADV
SNPEAWVEDALFRPETRESFQSLLGLGLTDALRAVNDAPGQYTFWDYQAGAWQKNAGIRIDHLLLSPQASDRLVEVGIDS
YVRAWEKPSDHVPVWADFDLETA
>Mature_263_residues
MRIATWNVNSIRQRIEHLVTWLKECSPDIVCLQEIKCTDDAFPRLEIEALGYNVVTHGQKTFNGVALLSKLPFDETKSGL
DGDAEDVHARFLEGVVSLKSGVVRIACLYLPNGNPPNTEKYPYKLKWMSRLRDYARERLKTEEPLILAGDFNVIPAAADV
SNPEAWVEDALFRPETRESFQSLLGLGLTDALRAVNDAPGQYTFWDYQAGAWQKNAGIRIDHLLLSPQASDRLVEVGIDS
YVRAWEKPSDHVPVWADFDLETA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=261, Percent_Identity=32.183908045977, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI18375503, Length=261, Percent_Identity=32.183908045977, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI18375501, Length=261, Percent_Identity=32.183908045977, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=35.7142857142857, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71989536, Length=271, Percent_Identity=28.7822878228782, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI221330655, Length=272, Percent_Identity=29.0441176470588, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17136678, Length=272, Percent_Identity=29.0441176470588, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29644; Mature: 29644

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATWNVNSIRQRIEHLVTWLKECSPDIVCLQEIKCTDDAFPRLEIEALGYNVVTHGQK
CEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCEEEECCCH
TFNGVALLSKLPFDETKSGLDGDAEDVHARFLEGVVSLKSGVVRIACLYLPNGNPPNTEK
HHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCC
YPYKLKWMSRLRDYARERLKTEEPLILAGDFNVIPAAADVSNPEAWVEDALFRPETRESF
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCHHHHHHHHCCCHHHHHH
QSLLGLGLTDALRAVNDAPGQYTFWDYQAGAWQKNAGIRIDHLLLSPQASDRLVEVGIDS
HHHHCCCHHHHHHHHCCCCCCEEEEECCCCCEECCCCCEEEEEEECCCCCCCEEECCHHH
YVRAWEKPSDHVPVWADFDLETA
HHHHHCCCCCCCCEEECCCCCCC
>Mature Secondary Structure
MRIATWNVNSIRQRIEHLVTWLKECSPDIVCLQEIKCTDDAFPRLEIEALGYNVVTHGQK
CEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCEEEECCCH
TFNGVALLSKLPFDETKSGLDGDAEDVHARFLEGVVSLKSGVVRIACLYLPNGNPPNTEK
HHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCC
YPYKLKWMSRLRDYARERLKTEEPLILAGDFNVIPAAADVSNPEAWVEDALFRPETRESF
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCHHHHHHHHCCCHHHHHH
QSLLGLGLTDALRAVNDAPGQYTFWDYQAGAWQKNAGIRIDHLLLSPQASDRLVEVGIDS
HHHHCCCHHHHHHHHCCCCCCEEEEECCCCCEECCCCCEEEEEEECCCCCCCEEECCHHH
YVRAWEKPSDHVPVWADFDLETA
HHHHHCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]