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The map label for this gene is yeaT [C]

Identifier: 146340987

GI number: 146340987

Start: 4247430

End: 4248329

Strand: Reverse

Name: yeaT [C]

Synonym: BRADO4058

Alternate gene names: 146340987

Gene position: 4248329-4247430 (Counterclockwise)

Preceding gene: 146340990

Following gene: 146340986

Centisome position: 56.97

GC content: 66.78

Gene sequence:

>900_bases
ATGGACCGGATCGACGCTTTGCAGACCTTCATCCGGGTCATGGAGGCCGGCAGCTTCACCCAAGCCGCGGCCGATCTCGG
CCTCGGTCAGCCCGCGATCACCAAGCGCATCGCGCTCCTGGAGGAGCAGTTCGGCTGCCGCCTGTTCGTCCGCACCACCC
GCAAGCTGCGCCCGACGCCTGAGGCCGATCGCGTTCTCAAGCTCGCCAAGGACATCGTGACCTTGTTCGAGAGCGCCGGC
GTGCCGTCGCCAAGGCGCGTCGGTGCCCCCTCCGGCACCTTGCGCATGACGGTGCCGACCTCGTTCGGCCGCTATTTCTT
CGCCGACATCTTTGCGGAGTACGGCCGCCGCTATCCCGATGTGCGGCTCGACATCCGCTTCACCGAGCAGGTCGTCGATC
TCGTCGAGACCGGCACCGAGCTTGCCATCCGCATCGGCGTACTGTCATCGAGCTCGCTGGTCGCGCGCCGTGTCGGCACC
GTCAAGCGGTTGCTGGCCGCCGCGCCATCGCTGCTCGCGCGGCATCGGCCGCCGCGTCTGCCGAACGATCTGCGCCAGCT
ACCCTGCGTGACCTATTCGCGGCTGTCGCCGAAGAATGAATGGAGCTTCGAGTCGGAGACCGGCCGACACGTCGTCGGCA
TCACGCCCACGCTCACCTGTGACGACGCAGACATCATGACTTTGGCCGCTCTCGAGGGCCTCGGCGCCGCCGTGCTGCCG
GACTGGTGCGCCGTCGAGCACATCGCGTCGGGCAGGCTCATCCATCTGCTGCCCGACTACGACGTCCCCTCGCTGCCGCT
TCACGTCGTTTATCCAGATCCACAATGGACGTCGCACCGGGCGCGCTTGTTTCGTGACCTGATCATCGAGCGCGCGGACG
TCTTCGCGATCGGCGGATAA

Upstream 100 bases:

>100_bases
CATGGGTCTCCCTCCGTGTGATTTTTTTGAGAGGCTAGACCACTCCTCCCGATCACGTATACGGAATGACAAGAATAAAA
CTCATTCCGGGGAGGAATGG

Downstream 100 bases:

>100_bases
CCAGGAGGGTGGGCAAGGCGCGCAGCGACGCGACAGCGTCGCGAGCACCGTGCCCACCGTCTCACCACGAACGTGCTGAA
CGATGGTGGGCACGGCGCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDRIDALQTFIRVMEAGSFTQAAADLGLGQPAITKRIALLEEQFGCRLFVRTTRKLRPTPEADRVLKLAKDIVTLFESAG
VPSPRRVGAPSGTLRMTVPTSFGRYFFADIFAEYGRRYPDVRLDIRFTEQVVDLVETGTELAIRIGVLSSSSLVARRVGT
VKRLLAAAPSLLARHRPPRLPNDLRQLPCVTYSRLSPKNEWSFESETGRHVVGITPTLTCDDADIMTLAALEGLGAAVLP
DWCAVEHIASGRLIHLLPDYDVPSLPLHVVYPDPQWTSHRARLFRDLIIERADVFAIGG

Sequences:

>Translated_299_residues
MDRIDALQTFIRVMEAGSFTQAAADLGLGQPAITKRIALLEEQFGCRLFVRTTRKLRPTPEADRVLKLAKDIVTLFESAG
VPSPRRVGAPSGTLRMTVPTSFGRYFFADIFAEYGRRYPDVRLDIRFTEQVVDLVETGTELAIRIGVLSSSSLVARRVGT
VKRLLAAAPSLLARHRPPRLPNDLRQLPCVTYSRLSPKNEWSFESETGRHVVGITPTLTCDDADIMTLAALEGLGAAVLP
DWCAVEHIASGRLIHLLPDYDVPSLPLHVVYPDPQWTSHRARLFRDLIIERADVFAIGG
>Mature_299_residues
MDRIDALQTFIRVMEAGSFTQAAADLGLGQPAITKRIALLEEQFGCRLFVRTTRKLRPTPEADRVLKLAKDIVTLFESAG
VPSPRRVGAPSGTLRMTVPTSFGRYFFADIFAEYGRRYPDVRLDIRFTEQVVDLVETGTELAIRIGVLSSSSLVARRVGT
VKRLLAAAPSLLARHRPPRLPNDLRQLPCVTYSRLSPKNEWSFESETGRHVVGITPTLTCDDADIMTLAALEGLGAAVLP
DWCAVEHIASGRLIHLLPDYDVPSLPLHVVYPDPQWTSHRARLFRDLIIERADVFAIGG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=285, Percent_Identity=32.280701754386, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786401, Length=287, Percent_Identity=29.616724738676, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI145693193, Length=286, Percent_Identity=30.4195804195804, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789440, Length=278, Percent_Identity=28.0575539568345, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787128, Length=285, Percent_Identity=29.4736842105263, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789639, Length=280, Percent_Identity=27.8571428571429, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=25.8620689655172, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788706, Length=285, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787879, Length=299, Percent_Identity=28.4280936454849, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=23.972602739726, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI157672245, Length=124, Percent_Identity=30.6451612903226, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787530, Length=264, Percent_Identity=23.1060606060606, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33141; Mature: 33141

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRIDALQTFIRVMEAGSFTQAAADLGLGQPAITKRIALLEEQFGCRLFVRTTRKLRPTP
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHCCCCC
EADRVLKLAKDIVTLFESAGVPSPRRVGAPSGTLRMTVPTSFGRYFFADIFAEYGRRYPD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCC
VRLDIRFTEQVVDLVETGTELAIRIGVLSSSSLVARRVGTVKRLLAAAPSLLARHRPPRL
EEEEEEHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PNDLRQLPCVTYSRLSPKNEWSFESETGRHVVGITPTLTCDDADIMTLAALEGLGAAVLP
CHHHHHCCCCHHHHCCCCCCCCCCCCCCCEEEEECCEEEECCCHHHHHHHHHCCCCHHCC
DWCAVEHIASGRLIHLLPDYDVPSLPLHVVYPDPQWTSHRARLFRDLIIERADVFAIGG
HHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCEEEECC
>Mature Secondary Structure
MDRIDALQTFIRVMEAGSFTQAAADLGLGQPAITKRIALLEEQFGCRLFVRTTRKLRPTP
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHCCCCC
EADRVLKLAKDIVTLFESAGVPSPRRVGAPSGTLRMTVPTSFGRYFFADIFAEYGRRYPD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCC
VRLDIRFTEQVVDLVETGTELAIRIGVLSSSSLVARRVGTVKRLLAAAPSLLARHRPPRL
EEEEEEHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PNDLRQLPCVTYSRLSPKNEWSFESETGRHVVGITPTLTCDDADIMTLAALEGLGAAVLP
CHHHHHCCCCHHHHCCCCCCCCCCCCCCCEEEEECCEEEECCCHHHHHHHHHCCCCHHCC
DWCAVEHIASGRLIHLLPDYDVPSLPLHVVYPDPQWTSHRARLFRDLIIERADVFAIGG
HHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]