Click here to switch to the map view.

The map label for this gene is pcm [H]

Identifier: 146340969

GI number: 146340969

Start: 4229256

End: 4229903

Strand: Reverse

Name: pcm [H]

Synonym: BRADO4040

Alternate gene names: 146340969

Gene position: 4229903-4229256 (Counterclockwise)

Preceding gene: 146340971

Following gene: 146340968

Centisome position: 56.73

GC content: 65.12

Gene sequence:

>648_bases
ATGCCCCCGACCGCTCCGCCCGAAAAGATGATGTTTCAGCTGATCTTGCGTCGGCGGGGTATCAGCGATCAGGCGGTGCT
GCGCGCCATGGAGGAGGTCCCCCGGGAAGAGTTCGTGCTGCCGGCCGACCGCGCCGACGCCTATCGTGACAGTGCAATGG
CGATCGCCTGCGGCCAGACCATCAGCCAACCCTTCGTGGTCGCCTACATGACTGAGCAGCTCAAGCTGCAGAAGAGCCAT
CGCGTGCTCGAAATCGGGACCGGCTCAGGCTACCAGGCCGCGGTCCTGGCTCGGCTGACCGACCATGTGCTGACAGTCGA
GCGTTTCCGGACGCTTGCCGATCGGGCGAGAGAGCGACTGGAGAAGCTGAACTGCTTCAACGTCGAGGTGATGCTCGGCG
ACGGCTACGCGTTACCGGCCGGGGCCGGCCAATTCGACCGCATCATTGTGACCGCGGCCATGGAAGACATCCCGCAGAGC
CTGCTCGACCGGCTGGAGCCGGACGGTATCCTGATCGCGCCTGTCGGACCTCACCACGGTGTCCAGCGGCTCGTGCGGGT
GACCCGGACGGGCGATCGCTTCGACCGCAAGGAGCTCGTCGACGTGCGATTTGTGCCCGCAATTCCGGGGATTGCGCGCG
AACTTTGA

Upstream 100 bases:

>100_bases
CTGCCATCAACGGGACGGCACCGAGGTTTGCTGATGAAAAGTCGGTGCGCAACGCTGGGGCGCCGTTTACATTGGCGCCA
CGCCAACCACCGACATCGCG

Downstream 100 bases:

>100_bases
ACGAGCGGATCAGCAAGTCTCCCAACGCTTTATTCGGGGCTTAAGCGCTTATTTACCGCGGCTGTGTTTACTCGAATTCG
TTGTTTGTTGCGTACGAGTG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MPPTAPPEKMMFQLILRRRGISDQAVLRAMEEVPREEFVLPADRADAYRDSAMAIACGQTISQPFVVAYMTEQLKLQKSH
RVLEIGTGSGYQAAVLARLTDHVLTVERFRTLADRARERLEKLNCFNVEVMLGDGYALPAGAGQFDRIIVTAAMEDIPQS
LLDRLEPDGILIAPVGPHHGVQRLVRVTRTGDRFDRKELVDVRFVPAIPGIAREL

Sequences:

>Translated_215_residues
MPPTAPPEKMMFQLILRRRGISDQAVLRAMEEVPREEFVLPADRADAYRDSAMAIACGQTISQPFVVAYMTEQLKLQKSH
RVLEIGTGSGYQAAVLARLTDHVLTVERFRTLADRARERLEKLNCFNVEVMLGDGYALPAGAGQFDRIIVTAAMEDIPQS
LLDRLEPDGILIAPVGPHHGVQRLVRVTRTGDRFDRKELVDVRFVPAIPGIAREL
>Mature_214_residues
PPTAPPEKMMFQLILRRRGISDQAVLRAMEEVPREEFVLPADRADAYRDSAMAIACGQTISQPFVVAYMTEQLKLQKSHR
VLEIGTGSGYQAAVLARLTDHVLTVERFRTLADRARERLEKLNCFNVEVMLGDGYALPAGAGQFDRIIVTAAMEDIPQSL
LDRLEPDGILIAPVGPHHGVQRLVRVTRTGDRFDRKELVDVRFVPAIPGIAREL

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=203, Percent_Identity=32.512315270936, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1789100, Length=195, Percent_Identity=49.2307692307692, Blast_Score=167, Evalue=3e-43,
Organism=Drosophila melanogaster, GI17981723, Length=202, Percent_Identity=28.2178217821782, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24001; Mature: 23870

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPTAPPEKMMFQLILRRRGISDQAVLRAMEEVPREEFVLPADRADAYRDSAMAIACGQT
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHEECCCCHHHHHHCCHHEEECCCC
ISQPFVVAYMTEQLKLQKSHRVLEIGTGSGYQAAVLARLTDHVLTVERFRTLADRARERL
CCCCEEEEHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKLNCFNVEVMLGDGYALPAGAGQFDRIIVTAAMEDIPQSLLDRLEPDGILIAPVGPHHG
HHCCCEEEEEEEECCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
VQRLVRVTRTGDRFDRKELVDVRFVPAIPGIAREL
HHHHHHHHHCCCCCCHHHHHCEEEECCCCHHHHCC
>Mature Secondary Structure 
PPTAPPEKMMFQLILRRRGISDQAVLRAMEEVPREEFVLPADRADAYRDSAMAIACGQT
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHEECCCCHHHHHHCCHHEEECCCC
ISQPFVVAYMTEQLKLQKSHRVLEIGTGSGYQAAVLARLTDHVLTVERFRTLADRARERL
CCCCEEEEHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKLNCFNVEVMLGDGYALPAGAGQFDRIIVTAAMEDIPQSLLDRLEPDGILIAPVGPHHG
HHCCCEEEEEEEECCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
VQRLVRVTRTGDRFDRKELVDVRFVPAIPGIAREL
HHHHHHHHHCCCCCCHHHHHCEEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA