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The map label for this gene is trmFO [H]

Identifier: 146340957

GI number: 146340957

Start: 4217475

End: 4218872

Strand: Direct

Name: trmFO [H]

Synonym: BRADO4028

Alternate gene names: 146340957

Gene position: 4217475-4218872 (Clockwise)

Preceding gene: 146340956

Following gene: 146340958

Centisome position: 56.56

GC content: 67.95

Gene sequence:

>1398_bases
GTGATCGGCGCCGGCCTCGCCGGCTCCGAGGCTGCGTGGCAACTCGCCGAGGCCGGCATCGACGTGGTGCTGCACGAGAT
GCGGCCGGACCGCATGACCGAGGCGCATCGCACGGCGACGCCCGCCGAGCTCGTGTGCTCCAATTCCTTCCGCTCCGACG
ATGCCGCCAACAATGCCGTCGGCCTGCTGCACGCCGAAATGCGCCGGCTCGGCTCGCTGATCATGCGGGCCGCCGATGCC
AACCAGGTCCCGGCCGGTGGCGCGCTGGCCGTCGACCGCGACAGCTTCGCGGCGGCCGTCGCAACGGCTCTGCAGGGCCA
TCCACGCATCGAGCTGCGCCGCGGCGAGATCACCGGCCTGCCTCCCGCCGAGTGGGCGAACGTGATAATCGCGACCGGCC
CCCTGACCTCCCAGCCGCTGGCCGACGCGATTCGCGCCCTGACCGACGAGAGCGCGCTCGCCTTCTTCGACGCCATTGCG
CCGATCGTGCACAAGGACACGATCGACATGTCGAAGGCCTGGTTCCAGTCGCGCTACGACAAGGTCGGTCCCGGTGGCAC
CGGCGCCGACTACATCAACTGCCCGATGACGCGGGAGCAGTACGACGCCTTCGTTGCCGCACTGCTCGCCGGCGAGAAGA
CCGATTTCAAGGACTGGGAGACGAATACGCCCTATTTCGACGGCTGCCTACCGATCGAAGTGATGGCCGAGCGCGGCCAC
GAGACCTTGCGCCACGGCCCGATGAAGCCAGTCGGCCTGACCAATCCGCACGATCCGACCGTGAAGCCGTATGCGATCGT
GCAGCTGCGCCAGGACAACAAGCTCGGCACGCTCTACAACATGGTCGGCTTCCAGACGAAGCTGAAATACGGCCCGCAGC
AGCAGATCTTCCGCACTATTCCCGGGCTTGAAAATGCGGAGTTTGCCCGGCTCGGCGGCCTGCACCGCAACACCTTCCTG
AATTCGCCGAAGCTGCTCGACCAGCAGCTACGCCTGCGGGCACAGCCGCGGCTGCGCTTCGCCGGCCAGATGACGGGCTG
CGAGGGCTATGTGGAGTCGGCGAGCATCGGTCTGATCGCCGGCCTCTACGCCGCTGCCGAGGCGCGTGGCGCCAGCCTCG
CCGCGCCGCCGCCGACCACCGCCCTCGGCGCCCTGCTCGGCCATATCACCGGCGGCCACATCGAAACGATCGATGGCGCG
ACGCGCTCGTTCCAGCCGATGAACATCAATTTCGGCCTGTTCCCGCCCTTGGCGGCGGCTCCGACCAAAAAGCCCGATGG
CACGCGACTGAAGGGCAACGAGAAGACGGTGGCCAAGAAGCAGGCGATGAGTGCGCGTGCGCTGGCGGATCTCGACCGCT
GGATCGCCGAGCATCTGCGCGTCGCAGCGGCTGCGTGA

Upstream 100 bases:

>100_bases
GTTTCTCGCCCTGACACTCACTCGCGCATTTTCACCGCCCGGGAAAAGCGCTACCTGTCCCGCGATGACAGGGACCAAAT
CACCTACGAATACCGTTCAC

Downstream 100 bases:

>100_bases
GATCGCGATGAGCCTGCCGAAGTCCGCCTCCGTCCTGCTGGCATCGCGCTGGAGCGAGGGCGTGCTGCTCAAGCGCGACG
TGTTCTCGACCGTCGAGCGC

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MIGAGLAGSEAAWQLAEAGIDVVLHEMRPDRMTEAHRTATPAELVCSNSFRSDDAANNAVGLLHAEMRRLGSLIMRAADA
NQVPAGGALAVDRDSFAAAVATALQGHPRIELRRGEITGLPPAEWANVIIATGPLTSQPLADAIRALTDESALAFFDAIA
PIVHKDTIDMSKAWFQSRYDKVGPGGTGADYINCPMTREQYDAFVAALLAGEKTDFKDWETNTPYFDGCLPIEVMAERGH
ETLRHGPMKPVGLTNPHDPTVKPYAIVQLRQDNKLGTLYNMVGFQTKLKYGPQQQIFRTIPGLENAEFARLGGLHRNTFL
NSPKLLDQQLRLRAQPRLRFAGQMTGCEGYVESASIGLIAGLYAAAEARGASLAAPPPTTALGALLGHITGGHIETIDGA
TRSFQPMNINFGLFPPLAAAPTKKPDGTRLKGNEKTVAKKQAMSARALADLDRWIAEHLRVAAAA

Sequences:

>Translated_465_residues
MIGAGLAGSEAAWQLAEAGIDVVLHEMRPDRMTEAHRTATPAELVCSNSFRSDDAANNAVGLLHAEMRRLGSLIMRAADA
NQVPAGGALAVDRDSFAAAVATALQGHPRIELRRGEITGLPPAEWANVIIATGPLTSQPLADAIRALTDESALAFFDAIA
PIVHKDTIDMSKAWFQSRYDKVGPGGTGADYINCPMTREQYDAFVAALLAGEKTDFKDWETNTPYFDGCLPIEVMAERGH
ETLRHGPMKPVGLTNPHDPTVKPYAIVQLRQDNKLGTLYNMVGFQTKLKYGPQQQIFRTIPGLENAEFARLGGLHRNTFL
NSPKLLDQQLRLRAQPRLRFAGQMTGCEGYVESASIGLIAGLYAAAEARGASLAAPPPTTALGALLGHITGGHIETIDGA
TRSFQPMNINFGLFPPLAAAPTKKPDGTRLKGNEKTVAKKQAMSARALADLDRWIAEHLRVAAAA
>Mature_465_residues
MIGAGLAGSEAAWQLAEAGIDVVLHEMRPDRMTEAHRTATPAELVCSNSFRSDDAANNAVGLLHAEMRRLGSLIMRAADA
NQVPAGGALAVDRDSFAAAVATALQGHPRIELRRGEITGLPPAEWANVIIATGPLTSQPLADAIRALTDESALAFFDAIA
PIVHKDTIDMSKAWFQSRYDKVGPGGTGADYINCPMTREQYDAFVAALLAGEKTDFKDWETNTPYFDGCLPIEVMAERGH
ETLRHGPMKPVGLTNPHDPTVKPYAIVQLRQDNKLGTLYNMVGFQTKLKYGPQQQIFRTIPGLENAEFARLGGLHRNTFL
NSPKLLDQQLRLRAQPRLRFAGQMTGCEGYVESASIGLIAGLYAAAEARGASLAAPPPTTALGALLGHITGGHIETIDGA
TRSFQPMNINFGLFPPLAAAPTKKPDGTRLKGNEKTVAKKQAMSARALADLDRWIAEHLRVAAAA

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 49927; Mature: 49927

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGAGLAGSEAAWQLAEAGIDVVLHEMRPDRMTEAHRTATPAELVCSNSFRSDDAANNAV
CCCCCCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHH
GLLHAEMRRLGSLIMRAADANQVPAGGALAVDRDSFAAAVATALQGHPRIELRRGEITGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEECCCCCCC
PPAEWANVIIATGPLTSQPLADAIRALTDESALAFFDAIAPIVHKDTIDMSKAWFQSRYD
CCHHHCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KVGPGGTGADYINCPMTREQYDAFVAALLAGEKTDFKDWETNTPYFDGCLPIEVMAERGH
CCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCH
ETLRHGPMKPVGLTNPHDPTVKPYAIVQLRQDNKLGTLYNMVGFQTKLKYGPQQQIFRTI
HHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHC
PGLENAEFARLGGLHRNTFLNSPKLLDQQLRLRAQPRLRFAGQMTGCEGYVESASIGLIA
CCCCCCCHHHHCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCHHHHHH
GLYAAAEARGASLAAPPPTTALGALLGHITGGHIETIDGATRSFQPMNINFGLFPPLAAA
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEECCCCCCCCCCCC
PTKKPDGTRLKGNEKTVAKKQAMSARALADLDRWIAEHLRVAAAA
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIGAGLAGSEAAWQLAEAGIDVVLHEMRPDRMTEAHRTATPAELVCSNSFRSDDAANNAV
CCCCCCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHH
GLLHAEMRRLGSLIMRAADANQVPAGGALAVDRDSFAAAVATALQGHPRIELRRGEITGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEECCCCCCC
PPAEWANVIIATGPLTSQPLADAIRALTDESALAFFDAIAPIVHKDTIDMSKAWFQSRYD
CCHHHCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KVGPGGTGADYINCPMTREQYDAFVAALLAGEKTDFKDWETNTPYFDGCLPIEVMAERGH
CCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCH
ETLRHGPMKPVGLTNPHDPTVKPYAIVQLRQDNKLGTLYNMVGFQTKLKYGPQQQIFRTI
HHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHC
PGLENAEFARLGGLHRNTFLNSPKLLDQQLRLRAQPRLRFAGQMTGCEGYVESASIGLIA
CCCCCCCHHHHCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCHHHHHH
GLYAAAEARGASLAAPPPTTALGALLGHITGGHIETIDGATRSFQPMNINFGLFPPLAAA
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEECCCCCCCCCCCC
PTKKPDGTRLKGNEKTVAKKQAMSARALADLDRWIAEHLRVAAAA
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA