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The map label for this gene is tgt [H]

Identifier: 146340919

GI number: 146340919

Start: 4181238

End: 4182392

Strand: Reverse

Name: tgt [H]

Synonym: BRADO3990

Alternate gene names: 146340919

Gene position: 4182392-4181238 (Counterclockwise)

Preceding gene: 146340920

Following gene: 146340917

Centisome position: 56.09

GC content: 69.7

Gene sequence:

>1155_bases
ATGAGCTCCCCGGCTAACGACCTTCCCAACCATTTCGAGCTACTCGGCACCGATGGTGCGGCCCGCACCGGCCGGCTGAC
CACACCGCATGGCGTGGTCCGCACCCCGGCCTTCATGCCGGTCGGCACCGCCGGCGCCATGAAGGGCATCCACTGGCGCG
ACGTGCGCGAGGCCGGCGCCGACATCGTGCTCGGCAACACCTATCACCTGATGCTGCGGCCGACCGCGGAGCGGATCGCC
GCGCTCGGCGGCCTGCAGCGCTTCACTGGCTGGGGCGGCCCGATGCTGACCGATTCCGGCGGATTCCAGGTGATGTCGCT
GTCGGGCTTGCGGAAGCTGACGGAGCAGGGGGTGACGTTCCGCTCGCATATCGACGGTGCCAAGATCGAGCTGACGCCGG
AGCGGGCGATCGAGGTGCAGCGCCTGTTCAACTCCGACATCGCGATGCAACTCGACGAATGCGTGCGGCTGCCGGCCGAG
CCCGCCGATGTCGAGCGCGCGATGCGGCTGTCGCTACGCTGGGCCGAGCGCTGCCGCCGCGCCTTCGAGAGTGCGCCCGA
CGGCTATATGCTGTTCGGCATCGTGCAGGGCGGCGACGTCCCCGCGCTGCGGGTGGCGAGCGCCGAGGCGCTGGTCGATA
TCGGCTTCCATGGCTATGCGATCGGCGGTCTCGCCGTCGGCGAGCCGCAGGCGGTGATGCTGGAGATGATCGAGGCCACC
GTGCCGGCGCTGCCGGCCGACCGGCCGCGTTATCTGATGGGCGTCGGCACGCCCGACGATCTGCTGGAGTCGGTGAAGCG
CGGCGTCGACATGTTCGACTGCGTGCTGCCGACCCGCAACGGCCGCCATGGCGTCGCCTATACGCGCTTCGGGCCGGTCA
ATCTGCGTAACGCGCGCCACGCCGACGACGCCAGGCCGCTGGACGAGGAAAGTCCGTGGGCGCCAGCGCGGACCTATGCA
CGCGCCTATCTGCACCATCTGATCAAGGCGGGCGAGGCGCTCGGCGCCACGCTGCTGTCGGAGATCAACATCGCCTATTT
CCAGCAGCTGATGGCCGGAATGCGCGAAGCCATCGCGCAGGGCCGCTTCACGGAGTTCTACGCGCGCACCAAGGCCGATT
GGGCCCGCGGCGACATCGCTCCGCGGGCCGCCTGA

Upstream 100 bases:

>100_bases
GGACCATAGGGACGGCCGGTTCCGCCGTCAACAGCCGATCACGGCGAAATCCCGCCGATCGCGGTTGAATCGGGCGCCAT
CCTGACGCAATAAGCCGGCC

Downstream 100 bases:

>100_bases
CGGCGCGCAAGACTCTGCGCGCGTTCTTTGCGGCGCATGATGCTGGGAATGTCGGGGAGATCGTCTGACGCCTTGCGGAT
CGTTCCGACGCGATGACAGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSSPANDLPNHFELLGTDGAARTGRLTTPHGVVRTPAFMPVGTAGAMKGIHWRDVREAGADIVLGNTYHLMLRPTAERIA
ALGGLQRFTGWGGPMLTDSGGFQVMSLSGLRKLTEQGVTFRSHIDGAKIELTPERAIEVQRLFNSDIAMQLDECVRLPAE
PADVERAMRLSLRWAERCRRAFESAPDGYMLFGIVQGGDVPALRVASAEALVDIGFHGYAIGGLAVGEPQAVMLEMIEAT
VPALPADRPRYLMGVGTPDDLLESVKRGVDMFDCVLPTRNGRHGVAYTRFGPVNLRNARHADDARPLDEESPWAPARTYA
RAYLHHLIKAGEALGATLLSEINIAYFQQLMAGMREAIAQGRFTEFYARTKADWARGDIAPRAA

Sequences:

>Translated_384_residues
MSSPANDLPNHFELLGTDGAARTGRLTTPHGVVRTPAFMPVGTAGAMKGIHWRDVREAGADIVLGNTYHLMLRPTAERIA
ALGGLQRFTGWGGPMLTDSGGFQVMSLSGLRKLTEQGVTFRSHIDGAKIELTPERAIEVQRLFNSDIAMQLDECVRLPAE
PADVERAMRLSLRWAERCRRAFESAPDGYMLFGIVQGGDVPALRVASAEALVDIGFHGYAIGGLAVGEPQAVMLEMIEAT
VPALPADRPRYLMGVGTPDDLLESVKRGVDMFDCVLPTRNGRHGVAYTRFGPVNLRNARHADDARPLDEESPWAPARTYA
RAYLHHLIKAGEALGATLLSEINIAYFQQLMAGMREAIAQGRFTEFYARTKADWARGDIAPRAA
>Mature_383_residues
SSPANDLPNHFELLGTDGAARTGRLTTPHGVVRTPAFMPVGTAGAMKGIHWRDVREAGADIVLGNTYHLMLRPTAERIAA
LGGLQRFTGWGGPMLTDSGGFQVMSLSGLRKLTEQGVTFRSHIDGAKIELTPERAIEVQRLFNSDIAMQLDECVRLPAEP
ADVERAMRLSLRWAERCRRAFESAPDGYMLFGIVQGGDVPALRVASAEALVDIGFHGYAIGGLAVGEPQAVMLEMIEATV
PALPADRPRYLMGVGTPDDLLESVKRGVDMFDCVLPTRNGRHGVAYTRFGPVNLRNARHADDARPLDEESPWAPARTYAR
AYLHHLIKAGEALGATLLSEINIAYFQQLMAGMREAIAQGRFTEFYARTKADWARGDIAPRAA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=346, Percent_Identity=43.9306358381503, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI13375872, Length=287, Percent_Identity=22.6480836236934, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1786607, Length=362, Percent_Identity=53.0386740331492, Blast_Score=381, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17544680, Length=364, Percent_Identity=40.1098901098901, Blast_Score=258, Evalue=3e-69,
Organism=Drosophila melanogaster, GI19920510, Length=362, Percent_Identity=44.475138121547, Blast_Score=295, Evalue=5e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41831; Mature: 41700

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPANDLPNHFELLGTDGAARTGRLTTPHGVVRTPAFMPVGTAGAMKGIHWRDVREAGA
CCCCCCCCCCHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCC
DIVLGNTYHLMLRPTAERIAALGGLQRFTGWGGPMLTDSGGFQVMSLSGLRKLTEQGVTF
CEEECCEEEEEECCHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEHHHHHHHHHCCCEE
RSHIDGAKIELTPERAIEVQRLFNSDIAMQLDECVRLPAEPADVERAMRLSLRWAERCRR
ECCCCCCEEEECHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AFESAPDGYMLFGIVQGGDVPALRVASAEALVDIGFHGYAIGGLAVGEPQAVMLEMIEAT
HHHCCCCCEEEEEEEECCCCCEEEECCHHHHEEECCCCEEECCEECCCCHHHHHHHHHHH
VPALPADRPRYLMGVGTPDDLLESVKRGVDMFDCVLPTRNGRHGVAYTRFGPVNLRNARH
CCCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEHHCCCCCCCCCEEEEEECCCCCCCCCC
ADDARPLDEESPWAPARTYARAYLHHLIKAGEALGATLLSEINIAYFQQLMAGMREAIAQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRFTEFYARTKADWARGDIAPRAA
CCHHHHHHHHCCCHHCCCCCCCCC
>Mature Secondary Structure 
SSPANDLPNHFELLGTDGAARTGRLTTPHGVVRTPAFMPVGTAGAMKGIHWRDVREAGA
CCCCCCCCCHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCC
DIVLGNTYHLMLRPTAERIAALGGLQRFTGWGGPMLTDSGGFQVMSLSGLRKLTEQGVTF
CEEECCEEEEEECCHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEHHHHHHHHHCCCEE
RSHIDGAKIELTPERAIEVQRLFNSDIAMQLDECVRLPAEPADVERAMRLSLRWAERCRR
ECCCCCCEEEECHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AFESAPDGYMLFGIVQGGDVPALRVASAEALVDIGFHGYAIGGLAVGEPQAVMLEMIEAT
HHHCCCCCEEEEEEEECCCCCEEEECCHHHHEEECCCCEEECCEECCCCHHHHHHHHHHH
VPALPADRPRYLMGVGTPDDLLESVKRGVDMFDCVLPTRNGRHGVAYTRFGPVNLRNARH
CCCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEHHCCCCCCCCCEEEEEECCCCCCCCCC
ADDARPLDEESPWAPARTYARAYLHHLIKAGEALGATLLSEINIAYFQQLMAGMREAIAQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRFTEFYARTKADWARGDIAPRAA
CCHHHHHHHHCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]