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The map label for this gene is cysK [H]

Identifier: 146340917

GI number: 146340917

Start: 4179251

End: 4180219

Strand: Reverse

Name: cysK [H]

Synonym: BRADO3987

Alternate gene names: 146340917

Gene position: 4180219-4179251 (Counterclockwise)

Preceding gene: 146340919

Following gene: 146340916

Centisome position: 56.06

GC content: 65.12

Gene sequence:

>969_bases
ATGGAGTCGTCGGCTGCGACACAGCGTCCCGGACGCGGGCGCGTATTTGATTCAATTATCGAGGCATTCGGCAATACGCC
GATTGTGCGGCTGCGAAAGTTGCCGGAGCAGCATGGCGTCAAGGCGACCATCCTCGCCAAGCTGGAATACTTCAATCCTG
CCGCGAGCGTGAAGGACCGCATTGGCGTCGGCATGATCCTGGCGATGGAGCAGGCCGGTGTGCTCAAGCCGGACTCCGTT
CTGATCGAGCCGACCTCGGGCAATACCGGCATTGCGCTCGCCTTCGTCGCGGCGGCGCGCGGCTACAAGCTGAAGCTTGT
CATGCCGGAGTCGATGTCGGTGGAGCGGCGCAAGATGCTTGCCTTCCTCGGCGCCGAGATCGTGCTGACACCGGCGGCGC
AGGGCATGAAGGGCGCGATCGCGACCGCCGAGGAGCTGTTGCGAACCACGCCGAACTCGGTGATGCCGCAGCAGTTCAAG
AACCTCGCCAATCCGGAGATCCATCGCCGCACGACGGCGGAGGAGATCTGGAACGACACCGCGGGGAATATCGACCTGTT
CGTCGCCGGCGTCGGTACCGGAGGCACCATTACCGGCGTCGGCCAGGTGCTGAAGTCGCGCAAACCCGGACTCAAGGTGG
TTGCTGTCGAGCCGGAGGAGAGCCCGGTCCTGTCGGGCGGCCAGCATTCGCCGCACAAGATCCAGGGCATCGGCGCCGGC
TTCGTCCCGGATATTCTCGACCGGTCCGTGATCGACGAGATCGTCAAGATCAACAGCGCCACGGCCATCGAGACCTCGCG
CGCGCTGGCGCGCAACGAGGGCATTCCCGGCGGCATTTCCTCGGGCGCGGCGATCGCGGCGGCGATCGAGCTCGGCAAGC
GGCCGGAGAATGCCGGCAAGACGATCCTGGCGATCGTCCCGTCGTTCTCCGAGCGCTATCTCTCCACGGCGCTGTTCGAA
GGGATCTGA

Upstream 100 bases:

>100_bases
CGATGATCATGCAGACGCGTAATCGTGCTTGCGCAGCGCAATAAGCTCGCTGAAAATGGTCAGGAACATTGTCGCGCAAA
AGTGCGCAAGGAGTGATCTC

Downstream 100 bases:

>100_bases
TCATGGCCGATCAGCCGCGACGTCCCCGCACGCTCGCCGACGCTCGCACCGAGGCGGAGGCTGCATTCAAGAAGGTGACC
ACCAAGCCGGTGGAGGCGCC

Product: O-acetylserine sulfhydrolase A

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MESSAATQRPGRGRVFDSIIEAFGNTPIVRLRKLPEQHGVKATILAKLEYFNPAASVKDRIGVGMILAMEQAGVLKPDSV
LIEPTSGNTGIALAFVAAARGYKLKLVMPESMSVERRKMLAFLGAEIVLTPAAQGMKGAIATAEELLRTTPNSVMPQQFK
NLANPEIHRRTTAEEIWNDTAGNIDLFVAGVGTGGTITGVGQVLKSRKPGLKVVAVEPEESPVLSGGQHSPHKIQGIGAG
FVPDILDRSVIDEIVKINSATAIETSRALARNEGIPGGISSGAAIAAAIELGKRPENAGKTILAIVPSFSERYLSTALFE
GI

Sequences:

>Translated_322_residues
MESSAATQRPGRGRVFDSIIEAFGNTPIVRLRKLPEQHGVKATILAKLEYFNPAASVKDRIGVGMILAMEQAGVLKPDSV
LIEPTSGNTGIALAFVAAARGYKLKLVMPESMSVERRKMLAFLGAEIVLTPAAQGMKGAIATAEELLRTTPNSVMPQQFK
NLANPEIHRRTTAEEIWNDTAGNIDLFVAGVGTGGTITGVGQVLKSRKPGLKVVAVEPEESPVLSGGQHSPHKIQGIGAG
FVPDILDRSVIDEIVKINSATAIETSRALARNEGIPGGISSGAAIAAAIELGKRPENAGKTILAIVPSFSERYLSTALFE
GI
>Mature_322_residues
MESSAATQRPGRGRVFDSIIEAFGNTPIVRLRKLPEQHGVKATILAKLEYFNPAASVKDRIGVGMILAMEQAGVLKPDSV
LIEPTSGNTGIALAFVAAARGYKLKLVMPESMSVERRKMLAFLGAEIVLTPAAQGMKGAIATAEELLRTTPNSVMPQQFK
NLANPEIHRRTTAEEIWNDTAGNIDLFVAGVGTGGTITGVGQVLKSRKPGLKVVAVEPEESPVLSGGQHSPHKIQGIGAG
FVPDILDRSVIDEIVKINSATAIETSRALARNEGIPGGISSGAAIAAAIELGKRPENAGKTILAIVPSFSERYLSTALFE
GI

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=313, Percent_Identity=39.9361022364217, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI295821200, Length=313, Percent_Identity=39.9361022364217, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI4557415, Length=313, Percent_Identity=39.9361022364217, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1788754, Length=315, Percent_Identity=53.6507936507937, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI2367138, Length=304, Percent_Identity=40.4605263157895, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17535051, Length=300, Percent_Identity=53.3333333333333, Blast_Score=317, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI17562970, Length=303, Percent_Identity=50.4950495049505, Blast_Score=294, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI32566674, Length=295, Percent_Identity=51.5254237288136, Blast_Score=289, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI115535073, Length=301, Percent_Identity=53.156146179402, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17561720, Length=300, Percent_Identity=49, Blast_Score=256, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI32566672, Length=215, Percent_Identity=50.6976744186047, Blast_Score=217, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI17534315, Length=330, Percent_Identity=39.3939393939394, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI25147552, Length=323, Percent_Identity=38.390092879257, Blast_Score=178, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17561716, Length=118, Percent_Identity=50, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=38.7301587301587, Blast_Score=205, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6321449, Length=338, Percent_Identity=35.207100591716, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24643623, Length=312, Percent_Identity=38.4615384615385, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI20129101, Length=312, Percent_Identity=38.4615384615385, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33922; Mature: 33922

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESSAATQRPGRGRVFDSIIEAFGNTPIVRLRKLPEQHGVKATILAKLEYFNPAASVKDR
CCCCCCCCCCCCCHHHHHHHHHHCCCCEEHHHHCCHHHCCEEEEEEEEHHCCCHHHHHHH
IGVGMILAMEQAGVLKPDSVLIEPTSGNTGIALAFVAAARGYKLKLVMPESMSVERRKML
CCCEEEEEECCCCCCCCCCEEEEECCCCCCCHHHEEHHCCCCEEEEECCCCCCHHHHHHH
AFLGAEIVLTPAAQGMKGAIATAEELLRTTPNSVMPQQFKNLANPEIHRRTTAEEIWNDT
HHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCC
AGNIDLFVAGVGTGGTITGVGQVLKSRKPGLKVVAVEPEESPVLSGGQHSPHKIQGIGAG
CCCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCC
FVPDILDRSVIDEIVKINSATAIETSRALARNEGIPGGISSGAAIAAAIELGKRPENAGK
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
TILAIVPSFSERYLSTALFEGI
EEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure
MESSAATQRPGRGRVFDSIIEAFGNTPIVRLRKLPEQHGVKATILAKLEYFNPAASVKDR
CCCCCCCCCCCCCHHHHHHHHHHCCCCEEHHHHCCHHHCCEEEEEEEEHHCCCHHHHHHH
IGVGMILAMEQAGVLKPDSVLIEPTSGNTGIALAFVAAARGYKLKLVMPESMSVERRKML
CCCEEEEEECCCCCCCCCCEEEEECCCCCCCHHHEEHHCCCCEEEEECCCCCCHHHHHHH
AFLGAEIVLTPAAQGMKGAIATAEELLRTTPNSVMPQQFKNLANPEIHRRTTAEEIWNDT
HHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCC
AGNIDLFVAGVGTGGTITGVGQVLKSRKPGLKVVAVEPEESPVLSGGQHSPHKIQGIGAG
CCCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCC
FVPDILDRSVIDEIVKINSATAIETSRALARNEGIPGGISSGAAIAAAIELGKRPENAGK
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
TILAIVPSFSERYLSTALFEGI
EEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]