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The map label for this gene is dppA [H]

Identifier: 146340891

GI number: 146340891

Start: 4139094

End: 4140710

Strand: Direct

Name: dppA [H]

Synonym: BRADO3957

Alternate gene names: 146340891

Gene position: 4139094-4140710 (Clockwise)

Preceding gene: 146340890

Following gene: 146340892

Centisome position: 55.51

GC content: 64.19

Gene sequence:

>1617_bases
ATGAATTCCAGCCGTTTCGAGATCACGCGCCGCACCGCCCTTCTGACCTCGGCCGCGATCGCCGCCAATGTCGCCAATCC
GCTGCGCGCTTTCGCGCAAGAGACGCCGCGCAAGGGCGGCGTGTTCAATGTGCATTACGGCGCCGAGCAGCGGCAGCTGA
ATCCGAGCCTGCAGGCCTCGACCGGCGTCTACATCATCGGCGGCAAGATCCAGGAGAACCTCGTCGACCTCGATGCCCAG
GGACAGCCGGTCGGCGTACTCGCCGAGAGCTGGGAGGCGACGCCCGACGGCAAGACCGTCACCTTCAAGCTGCGCAAGGG
GATCAGCTGGCACGACGGCAAGCCGTTCACGTCCGAAGACGTCGCCTTCACTGCCATGAACATGTGGAAGAAGATCCTGA
ACTATGGATCGACGCTGCAGCTGTTCCTCACGGCGGTCGATACGCCGGATCCGCAGACCGCGATCTTCCGCTACGAACGC
CCGATGCCGCTCAACCTGCTGCTGCGTGCTTTGCCCGATCTCGGCTACGTCTCGGCCAAGCACATCTACGAGTCCGGGGA
CATCAGGCAGAATCCGGCCAATCTCGCACCGGTCGGCACCGGTCCCTTCAAGTTCGTCAAGTACGAGCGCGGGCAATACA
TCATCGCCGAGCGCAACGCCGACTACTGGCGTCCGAACGCGCCATATCTCGATCGCATCGTCTGGAAGGTGATCACCGAC
CGTGCGGCCGCGGCCGCCCAGATGGAGGCCGGTGAGCTGCACTACAGCCCGTTCTCCGGACTGACCATCTCCGACATGGC
GCGTCTCGGCAAGGACAAGCGCTTCATCGTCTCGACCAAGGGCAATGAAGGCAACGCGCGCACCAATACGCTGGAATTCA
ACGTCCGCCGCAAGGAGCTGTCGGACGTGAAGGTCCGGCGCGCCATCGCGCATGCGATCAACGTGCCGTTCTTCATCGAG
AATTTCCTCGGTGACTTCGCCAAGCTCGGCACCGGGCCGATCCCGTCGACGTCGACCGACTTCTATCCCGGCGCCAACAC
GCCGCAATATCCCTACGATAAGGCCAAGGCCGCCGCGCTGCTCGACGAGGCCGGCTACAAGCCGGGTGCCGGCGGCGCGC
GCTTCTCGCTGAAGCTCCTGCCCGCGCCATGGGGCGAGGACATCTCGCTGTGGTCGACCTTCATCCAGCAGTCGCTCGGA
GAGGTCGGCATTGCGGTCGAGATCGTGCGCAACGATGGCGGCGGCTTCCTGAAGCAGGTCTATGACGAGCACGCCTTCGA
TCTCGCCACCGGCTGGCACCAGTACCGCAACGATCCTGCGGTCTCGACGACCGTGTGGTACCGCTCCGGCCAGCCGAAGG
GCGCGCCGTGGACCAACCAATGGGGCTGGGACGACGCCAAGATCGACAAGATCATCGATGATGCCGCGACCGAGGTCGAT
CCGGCCAAGCGCAAGGCGCTTTATGCCGACTTCGTCCGCGAGGCCAATACCGAGCTGCCGGTATGGATGCCGATCGAGCA
GATCTTCGTCACCGTGATCACCGCGAAGGCGCGCAACCATTCCAACACGCCGCGCTGGGGATCGTCGAGCTGGCACGATC
TCTGGCTGTCCGCCTGA

Upstream 100 bases:

>100_bases
TCCGGCGCGCAATCGGGAATGACTCGACGGCTCGATCCGCGTAAGCTTGACGCCCATTGCACCATCGGCCGGCTGTTGCC
TCGGCCTTCGGGAGACCTTG

Downstream 100 bases:

>100_bases
GCCGAACGCCGTCAGGATGAGGCCATGCGCATCTTGAGCCTTGCGGGGCGGCGGCTCGCCGCCTCGATTCCTACCCTGTT
CCTGATCCTGATCGGCGTGT

Product: putative ABC transporter extracellular substrate binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MNSSRFEITRRTALLTSAAIAANVANPLRAFAQETPRKGGVFNVHYGAEQRQLNPSLQASTGVYIIGGKIQENLVDLDAQ
GQPVGVLAESWEATPDGKTVTFKLRKGISWHDGKPFTSEDVAFTAMNMWKKILNYGSTLQLFLTAVDTPDPQTAIFRYER
PMPLNLLLRALPDLGYVSAKHIYESGDIRQNPANLAPVGTGPFKFVKYERGQYIIAERNADYWRPNAPYLDRIVWKVITD
RAAAAAQMEAGELHYSPFSGLTISDMARLGKDKRFIVSTKGNEGNARTNTLEFNVRRKELSDVKVRRAIAHAINVPFFIE
NFLGDFAKLGTGPIPSTSTDFYPGANTPQYPYDKAKAAALLDEAGYKPGAGGARFSLKLLPAPWGEDISLWSTFIQQSLG
EVGIAVEIVRNDGGGFLKQVYDEHAFDLATGWHQYRNDPAVSTTVWYRSGQPKGAPWTNQWGWDDAKIDKIIDDAATEVD
PAKRKALYADFVREANTELPVWMPIEQIFVTVITAKARNHSNTPRWGSSSWHDLWLSA

Sequences:

>Translated_538_residues
MNSSRFEITRRTALLTSAAIAANVANPLRAFAQETPRKGGVFNVHYGAEQRQLNPSLQASTGVYIIGGKIQENLVDLDAQ
GQPVGVLAESWEATPDGKTVTFKLRKGISWHDGKPFTSEDVAFTAMNMWKKILNYGSTLQLFLTAVDTPDPQTAIFRYER
PMPLNLLLRALPDLGYVSAKHIYESGDIRQNPANLAPVGTGPFKFVKYERGQYIIAERNADYWRPNAPYLDRIVWKVITD
RAAAAAQMEAGELHYSPFSGLTISDMARLGKDKRFIVSTKGNEGNARTNTLEFNVRRKELSDVKVRRAIAHAINVPFFIE
NFLGDFAKLGTGPIPSTSTDFYPGANTPQYPYDKAKAAALLDEAGYKPGAGGARFSLKLLPAPWGEDISLWSTFIQQSLG
EVGIAVEIVRNDGGGFLKQVYDEHAFDLATGWHQYRNDPAVSTTVWYRSGQPKGAPWTNQWGWDDAKIDKIIDDAATEVD
PAKRKALYADFVREANTELPVWMPIEQIFVTVITAKARNHSNTPRWGSSSWHDLWLSA
>Mature_538_residues
MNSSRFEITRRTALLTSAAIAANVANPLRAFAQETPRKGGVFNVHYGAEQRQLNPSLQASTGVYIIGGKIQENLVDLDAQ
GQPVGVLAESWEATPDGKTVTFKLRKGISWHDGKPFTSEDVAFTAMNMWKKILNYGSTLQLFLTAVDTPDPQTAIFRYER
PMPLNLLLRALPDLGYVSAKHIYESGDIRQNPANLAPVGTGPFKFVKYERGQYIIAERNADYWRPNAPYLDRIVWKVITD
RAAAAAQMEAGELHYSPFSGLTISDMARLGKDKRFIVSTKGNEGNARTNTLEFNVRRKELSDVKVRRAIAHAINVPFFIE
NFLGDFAKLGTGPIPSTSTDFYPGANTPQYPYDKAKAAALLDEAGYKPGAGGARFSLKLLPAPWGEDISLWSTFIQQSLG
EVGIAVEIVRNDGGGFLKQVYDEHAFDLATGWHQYRNDPAVSTTVWYRSGQPKGAPWTNQWGWDDAKIDKIIDDAATEVD
PAKRKALYADFVREANTELPVWMPIEQIFVTVITAKARNHSNTPRWGSSSWHDLWLSA

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=428, Percent_Identity=28.5046728971963, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1787052, Length=497, Percent_Identity=24.748490945674, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1787762, Length=414, Percent_Identity=25.3623188405797, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789887, Length=495, Percent_Identity=25.6565656565657, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787551, Length=521, Percent_Identity=24.7600767754319, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787495, Length=518, Percent_Identity=22.7799227799228, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59790; Mature: 59790

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSRFEITRRTALLTSAAIAANVANPLRAFAQETPRKGGVFNVHYGAEQRQLNPSLQAS
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCC
TGVYIIGGKIQENLVDLDAQGQPVGVLAESWEATPDGKTVTFKLRKGISWHDGKPFTSED
CCEEEECCCHHCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCC
VAFTAMNMWKKILNYGSTLQLFLTAVDTPDPQTAIFRYERPMPLNLLLRALPDLGYVSAK
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHH
HIYESGDIRQNPANLAPVGTGPFKFVKYERGQYIIAERNADYWRPNAPYLDRIVWKVITD
HHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
RAAAAAQMEAGELHYSPFSGLTISDMARLGKDKRFIVSTKGNEGNARTNTLEFNVRRKEL
HHHHHHHCCCCCEEECCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEEEHHHH
SDVKVRRAIAHAINVPFFIENFLGDFAKLGTGPIPSTSTDFYPGANTPQYPYDKAKAAAL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LDEAGYKPGAGGARFSLKLLPAPWGEDISLWSTFIQQSLGEVGIAVEIVRNDGGGFLKQV
HHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHH
YDEHAFDLATGWHQYRNDPAVSTTVWYRSGQPKGAPWTNQWGWDDAKIDKIIDDAATEVD
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
PAKRKALYADFVREANTELPVWMPIEQIFVTVITAKARNHSNTPRWGSSSWHDLWLSA
HHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MNSSRFEITRRTALLTSAAIAANVANPLRAFAQETPRKGGVFNVHYGAEQRQLNPSLQAS
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCC
TGVYIIGGKIQENLVDLDAQGQPVGVLAESWEATPDGKTVTFKLRKGISWHDGKPFTSED
CCEEEECCCHHCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCC
VAFTAMNMWKKILNYGSTLQLFLTAVDTPDPQTAIFRYERPMPLNLLLRALPDLGYVSAK
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHH
HIYESGDIRQNPANLAPVGTGPFKFVKYERGQYIIAERNADYWRPNAPYLDRIVWKVITD
HHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
RAAAAAQMEAGELHYSPFSGLTISDMARLGKDKRFIVSTKGNEGNARTNTLEFNVRRKEL
HHHHHHHCCCCCEEECCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEEEHHHH
SDVKVRRAIAHAINVPFFIENFLGDFAKLGTGPIPSTSTDFYPGANTPQYPYDKAKAAAL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LDEAGYKPGAGGARFSLKLLPAPWGEDISLWSTFIQQSLGEVGIAVEIVRNDGGGFLKQV
HHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHH
YDEHAFDLATGWHQYRNDPAVSTTVWYRSGQPKGAPWTNQWGWDDAKIDKIIDDAATEVD
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
PAKRKALYADFVREANTELPVWMPIEQIFVTVITAKARNHSNTPRWGSSSWHDLWLSA
HHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]